The Experts below are selected from a list of 123 Experts worldwide ranked by ideXlab platform

Steven J Mentzer - One of the best experts on this subject based on the ideXlab platform.

  • Stochastic Regulation of Cell Migration from the Efferent Lymph to Oxazolone-Stimulated Skin 1
    2008
    Co-Authors: Charles A West, James D Rawn, John B Hay, Scott Swanson, Steven J Mentzer
    Abstract:

    The systemic immune response is a dynamic process involving the trafficking of Lymphocytes from the Ag-stimulated Lymph Node to the peripheral tissue. Studies in sheep have demonstrated several phases of cell output in the efferent Lymph after Ag stimulation. When skin contact sensitizers are used as Ag, the efferent Lymph cell output peaks �96 h after Ag stimulation and is temporally associated with the recruitment of cells into the skin. To investigate the relative contribution of this high-output phase of efferent Lymphocytes to Lymphocytic inflammation in the skin, we used a common contact sensitizer 2-phenyl-4-ethoxymethylene-5-oxazolone (oxazolone) to stimulate the skin and draining Prescapular Lymph Node of adult sheep. The efferent Lymph ducts draining the Ag-stimulated and contralateral control Lymph Nodes were cannulated throughout the experimental period. The Lymphocytes leaving the Lymph Nodes during the 72-h period before maximum infiltration were differentially labeled with fluorescent tracers, reinjected into the arterial circulation, and tracked to the site of Ag stimulation. Quantitative tissue cytometry of the skin at the conclusion of the injection period (96 h after Ag stimulation) demonstrated more migratory cells derived from the Ag-stimulated Lymph Node than the contralateral control (median 18.5 vs 15.5 per field; p < 0.05). However, when corrected for total cell output of the Lymph Node, the Ag-stimulated migratory cells were 3.8-fold more prevalent in the skin than the contralateral control cells. These results suggest that the in situ immune response generally mirrors the frequency of recruitable Lymphocytes in the peripheral blood. The Journal of Immunology, 2001, 166: 1517–1523. The systemic immune response is a dynamic process involvin

  • stochastic regulation of cell migration from the efferent Lymph to oxazolone stimulated skin
    Journal of Immunology, 2001
    Co-Authors: Charles A West, James D Rawn, Scott J Swanson, John B Hay, Steven J Mentzer
    Abstract:

    The systemic immune response is a dynamic process involving the trafficking of Lymphocytes from the Ag-stimulated Lymph Node to the peripheral tissue. Studies in sheep have demonstrated several phases of cell output in the efferent Lymph after Ag stimulation. When skin contact sensitizers are used as Ag, the efferent Lymph cell output peaks ∼96 h after Ag stimulation and is temporally associated with the recruitment of cells into the skin. To investigate the relative contribution of this high-output phase of efferent Lymphocytes to Lymphocytic inflammation in the skin, we used a common contact sensitizer 2-phenyl-4-ethoxymethylene-5-oxazolone (oxazolone) to stimulate the skin and draining Prescapular Lymph Node of adult sheep. The efferent Lymph ducts draining the Ag-stimulated and contralateral control Lymph Nodes were cannulated throughout the experimental period. The Lymphocytes leaving the Lymph Nodes during the 72-h period before maximum infiltration were differentially labeled with fluorescent tracers, reinjected into the arterial circulation, and tracked to the site of Ag stimulation. Quantitative tissue cytometry of the skin at the conclusion of the injection period (96 h after Ag stimulation) demonstrated more migratory cells derived from the Ag-stimulated Lymph Node than the contralateral control (median 18.5 vs 15.5 per field; p

J Pega - One of the best experts on this subject based on the ideXlab platform.

  • systemic foot and mouth disease vaccination in cattle promotes specific antibody secreting cells at the respiratory tract and triggers local anamnestic responses upon aerosol infection
    Journal of Virology, 2015
    Co-Authors: J Pega, S Di Giacomo, Danilo Bucafusco, Juan Manuel Schammas, D A Malacari, F Barrionuevo, Alejandra Victoria Capozzo
    Abstract:

    ABSTRACT Foot-and-mouth disease (FMD) is a highly contagious viral disease affecting biungulate species. Commercial vaccines, formulated with inactivated FMD virus (FMDV), are regularly used worldwide to control the disease. Here, we studied the generation of antibody responses in local Lymphoid tissues along the respiratory system in vaccinated and further aerosol-infected cattle. Animals immunized with a high-payload monovalent FMD vaccine developed high titers of neutralizing antibodies at 7 days postvaccination (dpv), reaching a plateau at 29 dpv. FMDV-specific antibody-secreting cells (ASC), predominantly IgM, were evident at 7 dpv in the Prescapular Lymph Node (LN) draining the vaccination site and in distal LN draining the respiratory mucosa, although in lower numbers. At 29 dpv, a significant switch to IgG1 was clear in Prescapular LN, while FMDV-specific ASC were detected in all Lymphoid tissues draining the respiratory tract, mostly as IgM-secreting cells. None of the animals ( n = 10) exhibited FMD symptoms after oronasal challenge at 30 dpv. Three days postinfection, a large increase in ASC numbers and rapid isotype switches to IgG1 were observed, particularly in LN-draining virus replication sites already described. These results indicate for the first time that systemic FMD vaccination in cattle effectively promotes the presence of anti-FMDV ASC in Lymphoid tissues associated with the respiratory system. Oronasal infection triggered an immune reaction compatible with a local anamnestic response upon contact with the replicating FMDV, suggesting that FMD vaccination induces the circulation of virus-specific B Lymphocytes, including memory B cells that differentiate into ASC soon after contact with the infective virus. IMPORTANCE Over recent decades, world animal health organizations as well as national sanitary authorities have supported the use of vaccination as an essential component of the official FMD control programs in both endemic and disease-free settings. Very few works studied the local immunity induced by FMD vaccines at the respiratory mucosa, and local responses induced in vaccinated animals after aerosol infection have not been described yet. In this work, we demonstrate for the first time that systemic FMD vaccination (i) induced the early presence of active antigen-specific ASC along the respiratory tract and (ii) prompted a rapid local antibody response in the respiratory mucosa, triggered upon oronasal challenge and congruent with a memory B-cell response. This information may help to understand novel aspects of protective responses induced by current FMD vaccines as well as to provide alternative parameters to establish protection efficiency for new vaccine developments.

Alejandra Victoria Capozzo - One of the best experts on this subject based on the ideXlab platform.

  • systemic foot and mouth disease vaccination in cattle promotes specific antibody secreting cells at the respiratory tract and triggers local anamnestic responses upon aerosol infection
    Journal of Virology, 2015
    Co-Authors: J Pega, S Di Giacomo, Danilo Bucafusco, Juan Manuel Schammas, D A Malacari, F Barrionuevo, Alejandra Victoria Capozzo
    Abstract:

    ABSTRACT Foot-and-mouth disease (FMD) is a highly contagious viral disease affecting biungulate species. Commercial vaccines, formulated with inactivated FMD virus (FMDV), are regularly used worldwide to control the disease. Here, we studied the generation of antibody responses in local Lymphoid tissues along the respiratory system in vaccinated and further aerosol-infected cattle. Animals immunized with a high-payload monovalent FMD vaccine developed high titers of neutralizing antibodies at 7 days postvaccination (dpv), reaching a plateau at 29 dpv. FMDV-specific antibody-secreting cells (ASC), predominantly IgM, were evident at 7 dpv in the Prescapular Lymph Node (LN) draining the vaccination site and in distal LN draining the respiratory mucosa, although in lower numbers. At 29 dpv, a significant switch to IgG1 was clear in Prescapular LN, while FMDV-specific ASC were detected in all Lymphoid tissues draining the respiratory tract, mostly as IgM-secreting cells. None of the animals ( n = 10) exhibited FMD symptoms after oronasal challenge at 30 dpv. Three days postinfection, a large increase in ASC numbers and rapid isotype switches to IgG1 were observed, particularly in LN-draining virus replication sites already described. These results indicate for the first time that systemic FMD vaccination in cattle effectively promotes the presence of anti-FMDV ASC in Lymphoid tissues associated with the respiratory system. Oronasal infection triggered an immune reaction compatible with a local anamnestic response upon contact with the replicating FMDV, suggesting that FMD vaccination induces the circulation of virus-specific B Lymphocytes, including memory B cells that differentiate into ASC soon after contact with the infective virus. IMPORTANCE Over recent decades, world animal health organizations as well as national sanitary authorities have supported the use of vaccination as an essential component of the official FMD control programs in both endemic and disease-free settings. Very few works studied the local immunity induced by FMD vaccines at the respiratory mucosa, and local responses induced in vaccinated animals after aerosol infection have not been described yet. In this work, we demonstrate for the first time that systemic FMD vaccination (i) induced the early presence of active antigen-specific ASC along the respiratory tract and (ii) prompted a rapid local antibody response in the respiratory mucosa, triggered upon oronasal challenge and congruent with a memory B-cell response. This information may help to understand novel aspects of protective responses induced by current FMD vaccines as well as to provide alternative parameters to establish protection efficiency for new vaccine developments.

Charles A West - One of the best experts on this subject based on the ideXlab platform.

  • Stochastic Regulation of Cell Migration from the Efferent Lymph to Oxazolone-Stimulated Skin 1
    2008
    Co-Authors: Charles A West, James D Rawn, John B Hay, Scott Swanson, Steven J Mentzer
    Abstract:

    The systemic immune response is a dynamic process involving the trafficking of Lymphocytes from the Ag-stimulated Lymph Node to the peripheral tissue. Studies in sheep have demonstrated several phases of cell output in the efferent Lymph after Ag stimulation. When skin contact sensitizers are used as Ag, the efferent Lymph cell output peaks �96 h after Ag stimulation and is temporally associated with the recruitment of cells into the skin. To investigate the relative contribution of this high-output phase of efferent Lymphocytes to Lymphocytic inflammation in the skin, we used a common contact sensitizer 2-phenyl-4-ethoxymethylene-5-oxazolone (oxazolone) to stimulate the skin and draining Prescapular Lymph Node of adult sheep. The efferent Lymph ducts draining the Ag-stimulated and contralateral control Lymph Nodes were cannulated throughout the experimental period. The Lymphocytes leaving the Lymph Nodes during the 72-h period before maximum infiltration were differentially labeled with fluorescent tracers, reinjected into the arterial circulation, and tracked to the site of Ag stimulation. Quantitative tissue cytometry of the skin at the conclusion of the injection period (96 h after Ag stimulation) demonstrated more migratory cells derived from the Ag-stimulated Lymph Node than the contralateral control (median 18.5 vs 15.5 per field; p < 0.05). However, when corrected for total cell output of the Lymph Node, the Ag-stimulated migratory cells were 3.8-fold more prevalent in the skin than the contralateral control cells. These results suggest that the in situ immune response generally mirrors the frequency of recruitable Lymphocytes in the peripheral blood. The Journal of Immunology, 2001, 166: 1517–1523. The systemic immune response is a dynamic process involvin

  • stochastic regulation of cell migration from the efferent Lymph to oxazolone stimulated skin
    Journal of Immunology, 2001
    Co-Authors: Charles A West, James D Rawn, Scott J Swanson, John B Hay, Steven J Mentzer
    Abstract:

    The systemic immune response is a dynamic process involving the trafficking of Lymphocytes from the Ag-stimulated Lymph Node to the peripheral tissue. Studies in sheep have demonstrated several phases of cell output in the efferent Lymph after Ag stimulation. When skin contact sensitizers are used as Ag, the efferent Lymph cell output peaks ∼96 h after Ag stimulation and is temporally associated with the recruitment of cells into the skin. To investigate the relative contribution of this high-output phase of efferent Lymphocytes to Lymphocytic inflammation in the skin, we used a common contact sensitizer 2-phenyl-4-ethoxymethylene-5-oxazolone (oxazolone) to stimulate the skin and draining Prescapular Lymph Node of adult sheep. The efferent Lymph ducts draining the Ag-stimulated and contralateral control Lymph Nodes were cannulated throughout the experimental period. The Lymphocytes leaving the Lymph Nodes during the 72-h period before maximum infiltration were differentially labeled with fluorescent tracers, reinjected into the arterial circulation, and tracked to the site of Ag stimulation. Quantitative tissue cytometry of the skin at the conclusion of the injection period (96 h after Ag stimulation) demonstrated more migratory cells derived from the Ag-stimulated Lymph Node than the contralateral control (median 18.5 vs 15.5 per field; p

Danilo Bucafusco - One of the best experts on this subject based on the ideXlab platform.

  • systemic foot and mouth disease vaccination in cattle promotes specific antibody secreting cells at the respiratory tract and triggers local anamnestic responses upon aerosol infection
    Journal of Virology, 2015
    Co-Authors: J Pega, S Di Giacomo, Danilo Bucafusco, Juan Manuel Schammas, D A Malacari, F Barrionuevo, Alejandra Victoria Capozzo
    Abstract:

    ABSTRACT Foot-and-mouth disease (FMD) is a highly contagious viral disease affecting biungulate species. Commercial vaccines, formulated with inactivated FMD virus (FMDV), are regularly used worldwide to control the disease. Here, we studied the generation of antibody responses in local Lymphoid tissues along the respiratory system in vaccinated and further aerosol-infected cattle. Animals immunized with a high-payload monovalent FMD vaccine developed high titers of neutralizing antibodies at 7 days postvaccination (dpv), reaching a plateau at 29 dpv. FMDV-specific antibody-secreting cells (ASC), predominantly IgM, were evident at 7 dpv in the Prescapular Lymph Node (LN) draining the vaccination site and in distal LN draining the respiratory mucosa, although in lower numbers. At 29 dpv, a significant switch to IgG1 was clear in Prescapular LN, while FMDV-specific ASC were detected in all Lymphoid tissues draining the respiratory tract, mostly as IgM-secreting cells. None of the animals ( n = 10) exhibited FMD symptoms after oronasal challenge at 30 dpv. Three days postinfection, a large increase in ASC numbers and rapid isotype switches to IgG1 were observed, particularly in LN-draining virus replication sites already described. These results indicate for the first time that systemic FMD vaccination in cattle effectively promotes the presence of anti-FMDV ASC in Lymphoid tissues associated with the respiratory system. Oronasal infection triggered an immune reaction compatible with a local anamnestic response upon contact with the replicating FMDV, suggesting that FMD vaccination induces the circulation of virus-specific B Lymphocytes, including memory B cells that differentiate into ASC soon after contact with the infective virus. IMPORTANCE Over recent decades, world animal health organizations as well as national sanitary authorities have supported the use of vaccination as an essential component of the official FMD control programs in both endemic and disease-free settings. Very few works studied the local immunity induced by FMD vaccines at the respiratory mucosa, and local responses induced in vaccinated animals after aerosol infection have not been described yet. In this work, we demonstrate for the first time that systemic FMD vaccination (i) induced the early presence of active antigen-specific ASC along the respiratory tract and (ii) prompted a rapid local antibody response in the respiratory mucosa, triggered upon oronasal challenge and congruent with a memory B-cell response. This information may help to understand novel aspects of protective responses induced by current FMD vaccines as well as to provide alternative parameters to establish protection efficiency for new vaccine developments.