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Kusuma, Hermawan Andhika - One of the best experts on this subject based on the ideXlab platform.
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PERBEDAAN SENSITIVITAS METRONIDAZOL DAN MEROPENEM TERHADAP BAKTERI ANAEROB Prevotella melaninogenica PADA PASIEN NEKROSIS PULPA DI RSUD DR MOEWARDI SURAKARTA TAHUN 2015
2017Co-Authors: Kusuma, Hermawan AndhikaAbstract:Hermawan Andhika Kusuma, G0011107, 2015. Perbedaan Sensitivitas Metronidazol dan Meropenem terhadap Bakteri Anaerob Prevotella melaninogenica pada Pasien Nekrosis Pulpa di RSUD Dr. Moewardi Surakarta Tahun 2015. Skripsi. Fakultas Kedokteran Universitas Sebelas Maret, Surakarta. Latar Belakang: Di Indonesia, penyakit gigi dan mulut masuk dalam 10 besar penyakit yang banyak dikeluhkan masyarakat. Karies gigi merupakan penyakit gigi dan mulut yang banyak di derita. Karies gigi yang tidak dirawat menyebabkan nekrosis pulpa disertai infeksi bakteri anaerob seperti Prevotella melaninogenica. Salah satu pengobatan yang efektif untuk bakteri anaerob adalah metronidazol, namun beberapa penelitian melaporkan sudah terjadi penurunan resistensi metronidazol sebagai lini pertama terhadap bakteri anaerob. Metode Penelitian: Penelitian ini merupakan penelitian eksperimental dengan rancangan penelitian the post test only yang dilaksanakan pada Januari-Februari 2015. Penelitian dilakukan di Lab. Mikrobiologi RSUD Dr Moewardi dan Lab. Mikrobiologi FK UNS. Sampel diambil dengan metode convenience sampling sebanyak 9 isolat. Variabel bebas penelitian ini adalah antibiotik metronidazol dan meropenem. Variabel terikat penelitian ini diameter zona hambat metronidazol dan meropenem terhadap Prevotella melaninogenica. Hasil yang didapat diuji dengan uji Mann-Whitney. Hasil Penelitian: Hasil penelitian didapatkan nilai diameter zona hambat metronidazol dengan mean sebesar 66,84 mm dan Standar Deviasi 17,47. Sedangkan meropenem didapatkan nilai mean sebesar 65,1 mm dan Standar Deviasi 15,87. Hasil uji Mann-Whitney antara diameter zona hambat metronidazol dan meropenem terhadap bakteri Prevotella melaninogenica menunjukkan nilai p = 0,531. Simpulan Penelitian: Tidak terdapat perbedaan sensitivitas metronidazol dan meropenem terhadap bakteri anaerob Prevotella melaninogenica pada pasien nekrosis pulpa di RSUD Dr. Moewardi Surakarta tahun 2015
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PERBEDAAN SENSITIVITAS METRONIDAZOL DAN MEROPENEM TERHADAP BAKTERI ANAEROB Prevotella melaninogenica PADA PASIEN NEKROSIS PULPA DI RSUD DR MOEWARDI SURAKARTA TAHUN 2015
2015Co-Authors: Kusuma, Hermawan AndhikaAbstract:Latar Belakang: Di Indonesia, penyakit gigi dan mulut masuk dalam 10 besar penyakit yang banyak dikeluhkan masyarakat. Karies gigi merupakan penyakit gigi dan mulut yang banyak di derita. Karies gigi yang tidak dirawat menyebabkan nekrosis pulpa disertai infeksi bakteri anaerob seperti Prevotella melaninogenica. Salah satu pengobatan yang efektif untuk bakteri anaerob adalah metronidazol, namun beberapa penelitian melaporkan sudah terjadi penurunan resistensi metronidazol sebagai lini pertama terhadap bakteri anaerob. Metode Penelitian: Penelitian ini merupakan penelitian eksperimental dengan rancangan penelitian the post test only yang dilaksanakan pada Januari-Februari 2015. Penelitian dilakukan di Lab. Mikrobiologi RSUD Dr Moewardi dan Lab. Mikrobiologi FK UNS. Sampel diambil dengan metode convenience sampling sebanyak 9 isolat. Variabel bebas penelitian ini adalah antibiotik metronidazol dan meropenem. Variabel terikat penelitian ini diameter zona hambat metronidazol dan meropenem terhadap Prevotella melaninogenica. Hasil yang didapat diuji dengan uji Mann-Whitney. Hasil Penelitian: Hasil penelitian didapatkan nilai diameter zona hambat metronidazol dengan mean sebesar 66,84 mm dan Standar Deviasi 17,47. Sedangkan meropenem didapatkan nilai mean sebesar 65,1 mm dan Standar Deviasi 15,87. Hasil uji Mann-Whitney antara diameter zona hambat metronidazol dan meropenem terhadap bakteri Prevotella melaninogenica menunjukkan nilai p = 0,531. Simpulan Penelitian: Tidak terdapat perbedaan sensitivitas metronidazol dan meropenem terhadap bakteri anaerob Prevotella melaninogenica pada pasien nekrosis pulpa di RSUD Dr. Moewardi Surakarta tahun 2015. Kata Kunci: Sensitivitas, Metronidazol, Meropenem, Prevotella melaninogenic
Crystiane Venditi Gomes Do Amorim - One of the best experts on this subject based on the ideXlab platform.
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Susceptibilidade de alguns microrganismos orais frente à clorexidina e ao paramonoclorofenol
Sociedade Brasileira de Pesquisa Odontológica - SBPqO, 2004Co-Authors: Crystiane Venditi Gomes Do Amorim, Aun, Carlos Eduardo, Mayer, Marcia Pinto AlvesAbstract:Since the use of antimicrobial agents is required in endodontic therapies, this study aimed at determining the minimum inhibitory concentrations (MICs) of chlorhexidine digluconate and paramonochlorophenol (PMC) against microorganisms commonly found in endodontic infections. Both agents were tested by agar dilution tests against Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Candida albicans, Prevotella intermedia, Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella denticola and Prevotella melaninogenica. The MIC of chlorhexidine ranged from 2.67 to 80.00 µg/ml, and the MIC of PMC from 46.67 to 213.33 µg/ml. The highest MIC value of PMC was detected for E. faecalis whereas E. coli was the most susceptible microorganism to this agent. The highest MIC values of chlorhexidine were observed for P. aeruginosa whereas E. coli and P. denticola were the most susceptible microorganisms to this agent. Since the MIC values observed are much lower than the concentrations currently used in the endodontic therapy, it is suggested that both agents are effective in reducing the microbiota in the root canal.Tendo em vista a necessidade de se utilizarem agentes antimicrobianos durante a terapia endodôntica, o presente estudo tem por objetivo determinar as concentrações inibitórias mínimas (CIMs) de digluconato de clorexidina e de paramonoclorofenol (PMC) frente a cepas de microrganismos freqüentemente isolados dos canais radiculares infectados. Ambos os agentes foram testados por meio de testes de diluição em meio sólido contra Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Candida albicans, Prevotella intermedia, Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella denticola e Prevotella melaninogenica. As CIMs de clorexidina variaram de 2,67 a 80,00 µg/ml, e as CIMs de PMC variaram de 46,67 a 213,33 µg/ml. A maior CIM do paramonoclorofenol foi frente a E. faecalis, entretanto E. coli foi o microrganismo mais susceptível. A maior CIM do digluconato de clorexidina foi frente a P. aeruginosa, entretanto E. coli e P. denticola foram os microrganismos mais susceptíveis. Como os valores de CIM observados são bem menores do que as concentrações usadas normalmente na terapia endodôntica, sugere-se que ambos os agentes são efetivos na redução microbiana no canal radicular
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Susceptibility of some oral microorganisms to chlorhexidine and paramonochlorophenol
Sociedade Brasileira de Pesquisa Odontológica - SBPqO, 2004Co-Authors: Crystiane Venditi Gomes Do Amorim, Aun, Carlos Eduardo, Mayer, Marcia Pinto AlvesAbstract:Since the use of antimicrobial agents is required in endodontic therapies, this study aimed at determining the minimum inhibitory concentrations (MICs) of chlorhexidine digluconate and paramonochlorophenol (PMC) against microorganisms commonly found in endodontic infections. Both agents were tested by agar dilution tests against Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Candida albicans, Prevotella intermedia, Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella denticola and Prevotella melaninogenica. The MIC of chlorhexidine ranged from 2.67 to 80.00 µg/ml, and the MIC of PMC from 46.67 to 213.33 µg/ml. The highest MIC value of PMC was detected for E. faecalis whereas E. coli was the most susceptible microorganism to this agent. The highest MIC values of chlorhexidine were observed for P. aeruginosa whereas E. coli and P. denticola were the most susceptible microorganisms to this agent. Since the MIC values observed are much lower than the concentrations currently used in the endodontic therapy, it is suggested that both agents are effective in reducing the microbiota in the root canal
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Susceptibility of some oral microorganisms to chlorhexidine and paramonochlorophenol Susceptibilidade de alguns microrganismos orais frente à clorexidina e ao paramonoclorofenol
Sociedade Brasileira de Pesquisa Odontológica, 2004Co-Authors: Crystiane Venditi Gomes Do Amorim, Carlos Eduardo Aun, Marcia Pinto Alves MayerAbstract:Since the use of antimicrobial agents is required in endodontic therapies, this study aimed at determining the minimum inhibitory concentrations (MICs) of chlorhexidine digluconate and paramonochlorophenol (PMC) against microorganisms commonly found in endodontic infections. Both agents were tested by agar dilution tests against Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Candida albicans, Prevotella intermedia, Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella denticola and Prevotella melaninogenica. The MIC of chlorhexidine ranged from 2.67 to 80.00 µg/ml, and the MIC of PMC from 46.67 to 213.33 µg/ml. The highest MIC value of PMC was detected for E. faecalis whereas E. coli was the most susceptible microorganism to this agent. The highest MIC values of chlorhexidine were observed for P. aeruginosa whereas E. coli and P. denticola were the most susceptible microorganisms to this agent. Since the MIC values observed are much lower than the concentrations currently used in the endodontic therapy, it is suggested that both agents are effective in reducing the microbiota in the root canal.Tendo em vista a necessidade de se utilizarem agentes antimicrobianos durante a terapia endodôntica, o presente estudo tem por objetivo determinar as concentrações inibitórias mínimas (CIMs) de digluconato de clorexidina e de paramonoclorofenol (PMC) frente a cepas de microrganismos freqüentemente isolados dos canais radiculares infectados. Ambos os agentes foram testados por meio de testes de diluição em meio sólido contra Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Candida albicans, Prevotella intermedia, Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella denticola e Prevotella melaninogenica. As CIMs de clorexidina variaram de 2,67 a 80,00 µg/ml, e as CIMs de PMC variaram de 46,67 a 213,33 µg/ml. A maior CIM do paramonoclorofenol foi frente a E. faecalis, entretanto E. coli foi o microrganismo mais susceptível. A maior CIM do digluconato de clorexidina foi frente a P. aeruginosa, entretanto E. coli e P. denticola foram os microrganismos mais susceptíveis. Como os valores de CIM observados são bem menores do que as concentrações usadas normalmente na terapia endodôntica, sugere-se que ambos os agentes são efetivos na redução microbiana no canal radicular
Jia Zhou - One of the best experts on this subject based on the ideXlab platform.
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quantitative metagenomics reveals unique gut microbiome biomarkers in ankylosing spondylitis
Genome Biology, 2017Co-Authors: Chengping Wen, Zhijun Zheng, Tiejuan Shao, Lin Liu, Zhijun Xie, Wendi Zhong, Yongsheng Fan, Linshuang Zhang, Jia Zhou, Shunfeng CaiAbstract:The assessment and characterization of the gut microbiome has become a focus of research in the area of human autoimmune diseases. Ankylosing spondylitis is an inflammatory autoimmune disease and evidence showed that ankylosing spondylitis may be a microbiome-driven disease. To investigate the relationship between the gut microbiome and ankylosing spondylitis, a quantitative metagenomics study based on deep shotgun sequencing was performed, using gut microbial DNA from 211 Chinese individuals. A total of 23,709 genes and 12 metagenomic species were shown to be differentially abundant between ankylosing spondylitis patients and healthy controls. Patients were characterized by a form of gut microbial dysbiosis that is more prominent than previously reported cases with inflammatory bowel disease. Specifically, the ankylosing spondylitis patients demonstrated increases in the abundance of Prevotella melaninogenica, Prevotella copri, and Prevotella sp. C561 and decreases in Bacteroides spp. It is noteworthy that the Bifidobacterium genus, which is commonly used in probiotics, accumulated in the ankylosing spondylitis patients. Diagnostic algorithms were established using a subset of these gut microbial biomarkers. Alterations of the gut microbiome are associated with development of ankylosing spondylitis. Our data suggest biomarkers identified in this study might participate in the pathogenesis or development process of ankylosing spondylitis, providing new leads for the development of new diagnostic tools and potential treatments.
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Quantitative metagenomics reveals unique gut microbiome biomarkers in ankylosing spondylitis
Genome Biology, 2017Co-Authors: Chengping Wen, Zhijun Zheng, Tiejuan Shao, Lin Liu, Zhijun Xie, Wendi Zhong, Yongsheng Fan, Linshuang Zhang, Emmanuelle Le Chatelier, Jia ZhouAbstract:Background: The assessment and characterization of the gut microbiome has become a focus of research in the area of human autoimmune diseases. Ankylosing spondylitis is an inflammatory autoimmune disease and evidence showed that ankylosing spondylitis may be a microbiome-driven disease. Results: To investigate the relationship between the gut microbiome and ankylosing spondylitis, a quantitative metagenomics study based on deep shotgun sequencing was performed, using gut microbial DNA from 211 Chinese individuals. A total of 23,709 genes and 12 metagenomic species were shown to be differentially abundant between ankylosing spondylitis patients and healthy controls. Patients were characterized by a form of gut microbial dysbiosis that is more prominent than previously reported cases with inflammatory bowel disease. Specifically, the ankylosing spondylitis patients demonstrated increases in the abundance of Prevotella melaninogenica, Prevotella copri, and Prevotella sp. C561 and decreases in Bacteroides spp. It is noteworthy that the Bifidobacterium genus, which is commonly used in probiotics, accumulated in the ankylosing spondylitis patients. Diagnostic algorithms were established using a subset of these gut microbial biomarkers. Conclusions: Alterations of the gut microbiome are associated with development of ankylosing spondylitis. Our data suggest biomarkers identified in this study might participate in the pathogenesis or development process of ankylosing spondylitis, providing new leads for the development of new diagnostic tools and potential treatments.
Chengping Wen - One of the best experts on this subject based on the ideXlab platform.
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quantitative metagenomics reveals unique gut microbiome biomarkers in ankylosing spondylitis
Genome Biology, 2017Co-Authors: Chengping Wen, Zhijun Zheng, Tiejuan Shao, Lin Liu, Zhijun Xie, Wendi Zhong, Yongsheng Fan, Linshuang Zhang, Jia Zhou, Shunfeng CaiAbstract:The assessment and characterization of the gut microbiome has become a focus of research in the area of human autoimmune diseases. Ankylosing spondylitis is an inflammatory autoimmune disease and evidence showed that ankylosing spondylitis may be a microbiome-driven disease. To investigate the relationship between the gut microbiome and ankylosing spondylitis, a quantitative metagenomics study based on deep shotgun sequencing was performed, using gut microbial DNA from 211 Chinese individuals. A total of 23,709 genes and 12 metagenomic species were shown to be differentially abundant between ankylosing spondylitis patients and healthy controls. Patients were characterized by a form of gut microbial dysbiosis that is more prominent than previously reported cases with inflammatory bowel disease. Specifically, the ankylosing spondylitis patients demonstrated increases in the abundance of Prevotella melaninogenica, Prevotella copri, and Prevotella sp. C561 and decreases in Bacteroides spp. It is noteworthy that the Bifidobacterium genus, which is commonly used in probiotics, accumulated in the ankylosing spondylitis patients. Diagnostic algorithms were established using a subset of these gut microbial biomarkers. Alterations of the gut microbiome are associated with development of ankylosing spondylitis. Our data suggest biomarkers identified in this study might participate in the pathogenesis or development process of ankylosing spondylitis, providing new leads for the development of new diagnostic tools and potential treatments.
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Quantitative metagenomics reveals unique gut microbiome biomarkers in ankylosing spondylitis
Genome Biology, 2017Co-Authors: Chengping Wen, Zhijun Zheng, Tiejuan Shao, Lin Liu, Zhijun Xie, Wendi Zhong, Yongsheng Fan, Linshuang Zhang, Emmanuelle Le Chatelier, Jia ZhouAbstract:Background: The assessment and characterization of the gut microbiome has become a focus of research in the area of human autoimmune diseases. Ankylosing spondylitis is an inflammatory autoimmune disease and evidence showed that ankylosing spondylitis may be a microbiome-driven disease. Results: To investigate the relationship between the gut microbiome and ankylosing spondylitis, a quantitative metagenomics study based on deep shotgun sequencing was performed, using gut microbial DNA from 211 Chinese individuals. A total of 23,709 genes and 12 metagenomic species were shown to be differentially abundant between ankylosing spondylitis patients and healthy controls. Patients were characterized by a form of gut microbial dysbiosis that is more prominent than previously reported cases with inflammatory bowel disease. Specifically, the ankylosing spondylitis patients demonstrated increases in the abundance of Prevotella melaninogenica, Prevotella copri, and Prevotella sp. C561 and decreases in Bacteroides spp. It is noteworthy that the Bifidobacterium genus, which is commonly used in probiotics, accumulated in the ankylosing spondylitis patients. Diagnostic algorithms were established using a subset of these gut microbial biomarkers. Conclusions: Alterations of the gut microbiome are associated with development of ankylosing spondylitis. Our data suggest biomarkers identified in this study might participate in the pathogenesis or development process of ankylosing spondylitis, providing new leads for the development of new diagnostic tools and potential treatments.
Mayer, Marcia Pinto Alves - One of the best experts on this subject based on the ideXlab platform.
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Susceptibilidade de alguns microrganismos orais frente à clorexidina e ao paramonoclorofenol
Sociedade Brasileira de Pesquisa Odontológica - SBPqO, 2004Co-Authors: Crystiane Venditi Gomes Do Amorim, Aun, Carlos Eduardo, Mayer, Marcia Pinto AlvesAbstract:Since the use of antimicrobial agents is required in endodontic therapies, this study aimed at determining the minimum inhibitory concentrations (MICs) of chlorhexidine digluconate and paramonochlorophenol (PMC) against microorganisms commonly found in endodontic infections. Both agents were tested by agar dilution tests against Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Candida albicans, Prevotella intermedia, Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella denticola and Prevotella melaninogenica. The MIC of chlorhexidine ranged from 2.67 to 80.00 µg/ml, and the MIC of PMC from 46.67 to 213.33 µg/ml. The highest MIC value of PMC was detected for E. faecalis whereas E. coli was the most susceptible microorganism to this agent. The highest MIC values of chlorhexidine were observed for P. aeruginosa whereas E. coli and P. denticola were the most susceptible microorganisms to this agent. Since the MIC values observed are much lower than the concentrations currently used in the endodontic therapy, it is suggested that both agents are effective in reducing the microbiota in the root canal.Tendo em vista a necessidade de se utilizarem agentes antimicrobianos durante a terapia endodôntica, o presente estudo tem por objetivo determinar as concentrações inibitórias mínimas (CIMs) de digluconato de clorexidina e de paramonoclorofenol (PMC) frente a cepas de microrganismos freqüentemente isolados dos canais radiculares infectados. Ambos os agentes foram testados por meio de testes de diluição em meio sólido contra Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Candida albicans, Prevotella intermedia, Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella denticola e Prevotella melaninogenica. As CIMs de clorexidina variaram de 2,67 a 80,00 µg/ml, e as CIMs de PMC variaram de 46,67 a 213,33 µg/ml. A maior CIM do paramonoclorofenol foi frente a E. faecalis, entretanto E. coli foi o microrganismo mais susceptível. A maior CIM do digluconato de clorexidina foi frente a P. aeruginosa, entretanto E. coli e P. denticola foram os microrganismos mais susceptíveis. Como os valores de CIM observados são bem menores do que as concentrações usadas normalmente na terapia endodôntica, sugere-se que ambos os agentes são efetivos na redução microbiana no canal radicular
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Susceptibility of some oral microorganisms to chlorhexidine and paramonochlorophenol
Sociedade Brasileira de Pesquisa Odontológica - SBPqO, 2004Co-Authors: Crystiane Venditi Gomes Do Amorim, Aun, Carlos Eduardo, Mayer, Marcia Pinto AlvesAbstract:Since the use of antimicrobial agents is required in endodontic therapies, this study aimed at determining the minimum inhibitory concentrations (MICs) of chlorhexidine digluconate and paramonochlorophenol (PMC) against microorganisms commonly found in endodontic infections. Both agents were tested by agar dilution tests against Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Candida albicans, Prevotella intermedia, Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella denticola and Prevotella melaninogenica. The MIC of chlorhexidine ranged from 2.67 to 80.00 µg/ml, and the MIC of PMC from 46.67 to 213.33 µg/ml. The highest MIC value of PMC was detected for E. faecalis whereas E. coli was the most susceptible microorganism to this agent. The highest MIC values of chlorhexidine were observed for P. aeruginosa whereas E. coli and P. denticola were the most susceptible microorganisms to this agent. Since the MIC values observed are much lower than the concentrations currently used in the endodontic therapy, it is suggested that both agents are effective in reducing the microbiota in the root canal