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Marika Mikelsaar - One of the best experts on this subject based on the ideXlab platform.
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Impact of Probiotic Lactobacillus plantarum TENSIA in different dairy products on anthropometric and blood biochemical indices of healthy adults
Beneficial Microbes, 2020Co-Authors: Pirje Hutt, Epp Songisepp, Merle Ratsep, Riina Mahlapuu, Kalle Kilk, Marika MikelsaarAbstract:The blood pressure-lowering effect of dairy products holds the potential to decrease the risk of cardiovascular disease (CVD). An open question is if the successful expression of functional properties of the Probiotic strain depends on host biomarkers and/or food matrix properties. The Probiotic Lactobacillus plantarum strain TENSIA® (DSM 21380) is a novel microorganism with antimicrobial and antihypertensive functional properties. The aim of this study was to characterise the functional properties of the Probiotic L. plantarum TENSIA and compare its effects on host anthropometric, clinical, and blood biomarkers when consumed with Cheese or yoghurt. This study involved two double-blinded randomised placebo-controlled exploratory trials (ISRCTN15061552 and ISRCTN79645828) of healthy adults over a three-week period. The three-week consumption of Probiotic L. plantarum TENSIA in a daily dose of 1×1010 cfu in Probiotic Cheese or a daily dose of 6×109 cfu in yoghurt with different content of carbohydrates, pro...
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Hypocaloric diet supplemented with Probiotic Cheese improves body mass index and blood pressure indices of obese hypertensive patients - a randomized double-blind placebo-controlled pilot study
Nutrition Journal, 2013Co-Authors: Khaider K. Sharafedtinov, Oksana A. Plotnikova, Ravilay I. Alexeeva, Tatjana B. Sentsova, Epp Songisepp, Jelena Stsepetova, Imbi Smidt, Marika MikelsaarAbstract:Background Gut lactobacilli can affect the metabolic functions of healthy humans. We tested whether a 1500 kcal/d diet supplemented with Cheese containing the Probiotic Lactobacillus plantarum TENSIA (Deutsche Sammlung für Mikroorganismen, DSM 21380) could reduce some symptoms of metabolic syndrome in Russian adults with obesity and hypertension. Methods In this 3-week, randomized, double-blind, placebo-controlled, parallel pilot study, 25 subjects ingested Probiotic Cheese and 15 ingested control Cheese. Fifty grams of each Cheese provided 175 kcal of energy. Blood pressure (BP), anthropometric characteristics, markers of liver and kidney function, metabolic indices (plasma glucose, lipids, and cholesterol), and urine polyamines were measured. Counts of fecal lactobacilli and L . plantarum TENSIA were evaluated using molecular methods. The data were analyzed by t-test for independent samples and Spearman’s partial correlation analysis. Results The Probiotic L. plantarum TENSIA was present in variable amounts (529.6 ± 232.5 gene copies) in 16/25 (64%) study subjects. Body mass index (BMI) was significantly reduced (p = 0.031) in the Probiotic Cheese group versus the control Cheese group. The changes in BMI were closely associated with the water content of the body (r = 0.570, p = 0.0007) when adjusted for sex and age. Higher values of intestinal lactobacilli after Probiotic Cheese consumption were associated with higher BMI (r = 0.383, p = 0.0305) and urinary putrescine content (r = 0.475, p = 0.006). In patients simultaneously treated with BP-lowering drugs, similar reductions of BP were observed in both groups. A positive association was detected between TENSIA colonization and the extent of change of morning diastolic BP (r = 0.617, p = 0.0248) and a trend toward lower values of morning systolic BP (r = −0.527, p = 0.0640) at the end of the study after adjusting for BMI, age, and sex. Conclusion In a pilot study of obese hypertensive patients, a hypocaloric diet supplemented with a Probiotic Cheese helps to reduce BMI and arterial BP values, recognized symptoms of metabolic syndrome. Trial registration Current Controlled Trials ISRCTN76271778
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hypocaloric diet supplemented with Probiotic Cheese improves body mass index and blood pressure indices of obese hypertensive patients a randomized double blind placebo controlled pilot study
Nutrition Journal, 2013Co-Authors: Khaider K. Sharafedtinov, Oksana A. Plotnikova, Ravilay I. Alexeeva, Tatjana B. Sentsova, Epp Songisepp, Jelena Stsepetova, Imbi Smidt, Marika MikelsaarAbstract:Background Gut lactobacilli can affect the metabolic functions of healthy humans. We tested whether a 1500 kcal/d diet supplemented with Cheese containing the Probiotic Lactobacillus plantarum TENSIA (Deutsche Sammlung fur Mikroorganismen, DSM 21380) could reduce some symptoms of metabolic syndrome in Russian adults with obesity and hypertension.
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Hypocaloric diet supplemented with Probiotic Cheese improves body mass index and blood pressure indices of obese hypertensive patients - A randomized double-blind placebo-controlled pilot study
Nutrition Journal, 2013Co-Authors: Khaider K. Sharafedtinov, Oksana A. Plotnikova, Ravilay I. Alexeeva, Tatjana B. Sentsova, Epp Songisepp, Jelena Stsepetova, Imbi Smidt, Marika MikelsaarAbstract:BACKGROUND: Gut lactobacilli can affect the metabolic functions of healthy humans. We tested whether a 1500 kcal/d diet supplemented with Cheese containing the Probiotic Lactobacillus plantarum TENSIA (Deutsche Sammlung für Mikroorganismen, DSM 21380) could reduce some symptoms of metabolic syndrome in Russian adults with obesity and hypertension.\n\nMETHODS: In this 3-week, randomized, double-blind, placebo-controlled, parallel pilot study, 25 subjects ingested Probiotic Cheese and 15 ingested control Cheese. Fifty grams of each Cheese provided 175 kcal of energy. Blood pressure (BP), anthropometric characteristics, markers of liver and kidney function, metabolic indices (plasma glucose, lipids, and cholesterol), and urine polyamines were measured. Counts of fecal lactobacilli and L. plantarum TENSIA were evaluated using molecular methods. The data were analyzed by t-test for independent samples and Spearman's partial correlation analysis.\n\nRESULTS: The Probiotic L. plantarum TENSIA was present in variable amounts (529.6 ± 232.5 gene copies) in 16/25 (64%) study subjects. Body mass index (BMI) was significantly reduced (p = 0.031) in the Probiotic Cheese group versus the control Cheese group. The changes in BMI were closely associated with the water content of the body (r = 0.570, p = 0.0007) when adjusted for sex and age. Higher values of intestinal lactobacilli after Probiotic Cheese consumption were associated with higher BMI (r = 0.383, p = 0.0305) and urinary putrescine content (r = 0.475, p = 0.006). In patients simultaneously treated with BP-lowering drugs, similar reductions of BP were observed in both groups. A positive association was detected between TENSIA colonization and the extent of change of morning diastolic BP (r = 0.617, p = 0.0248) and a trend toward lower values of morning systolic BP (r = -0.527, p = 0.0640) at the end of the study after adjusting for BMI, age, and sex.\n\nCONCLUSION: In a pilot study of obese hypertensive patients, a hypocaloric diet supplemented with a Probiotic Cheese helps to reduce BMI and arterial BP values, recognized symptoms of metabolic syndrome.
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a new Probiotic Cheese with antioxidative and antimicrobial activity
Journal of Dairy Science, 2004Co-Authors: Epp Songisepp, Tiiu Kullisaar, Pirje Hutt, P Elias, T Brilene, Mihkel Zilmer, Marika MikelsaarAbstract:Abstract The aim of our study was to develop an original Probiotic Cheese based on the Estonian open-texture, smear-ripened, semisoft Cheese "Pikantne." Cheese was produced by two methods using Cheese starter cultures (Probat 505) in combination with 0.04% of Probiotic Lactobacillus fermentum strain ME-3 (10 9 cfu/mL) with high antimicrobial activity and antioxidative properties. The Probiotic Lactobacillus was added into milk simultaneously with starter cultures (Cheese A) and into drained curd (Cheese B). After addition of Probiotic L. fermentum ME-3, the Cheese composition, flavor, and aroma were comparable to the control Cheese (score values=4.5, 4.2, and 3.7 for control Cheese, Cheese A, and Cheese B, respectively). Cheese A, which had good sensory properties, was chosen for further testing of viability and Probiotic properties. The Probiotic strain was found to withstand the technological processing of Cheese, surviving and sustaining moderate antimicrobial and high antioxidative activity throughout ripening and storage (the ripened Cheese contained approximately 5×10 7 cfu/g viable ME-3 cells), although the viability of the ME-3 strain incorporated into the Cheese showed a slight decrease between d 24 and 54 after Cheese preparation. Semisoft Cheese "Pikantne" serves as a suitable carrier of antimicrobial and antioxidative L. fermentum ME-3.
Epp Songisepp - One of the best experts on this subject based on the ideXlab platform.
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Impact of Probiotic Lactobacillus plantarum TENSIA in different dairy products on anthropometric and blood biochemical indices of healthy adults
Beneficial Microbes, 2020Co-Authors: Pirje Hutt, Epp Songisepp, Merle Ratsep, Riina Mahlapuu, Kalle Kilk, Marika MikelsaarAbstract:The blood pressure-lowering effect of dairy products holds the potential to decrease the risk of cardiovascular disease (CVD). An open question is if the successful expression of functional properties of the Probiotic strain depends on host biomarkers and/or food matrix properties. The Probiotic Lactobacillus plantarum strain TENSIA® (DSM 21380) is a novel microorganism with antimicrobial and antihypertensive functional properties. The aim of this study was to characterise the functional properties of the Probiotic L. plantarum TENSIA and compare its effects on host anthropometric, clinical, and blood biomarkers when consumed with Cheese or yoghurt. This study involved two double-blinded randomised placebo-controlled exploratory trials (ISRCTN15061552 and ISRCTN79645828) of healthy adults over a three-week period. The three-week consumption of Probiotic L. plantarum TENSIA in a daily dose of 1×1010 cfu in Probiotic Cheese or a daily dose of 6×109 cfu in yoghurt with different content of carbohydrates, pro...
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Hypocaloric diet supplemented with Probiotic Cheese improves body mass index and blood pressure indices of obese hypertensive patients - a randomized double-blind placebo-controlled pilot study
Nutrition Journal, 2013Co-Authors: Khaider K. Sharafedtinov, Oksana A. Plotnikova, Ravilay I. Alexeeva, Tatjana B. Sentsova, Epp Songisepp, Jelena Stsepetova, Imbi Smidt, Marika MikelsaarAbstract:Background Gut lactobacilli can affect the metabolic functions of healthy humans. We tested whether a 1500 kcal/d diet supplemented with Cheese containing the Probiotic Lactobacillus plantarum TENSIA (Deutsche Sammlung für Mikroorganismen, DSM 21380) could reduce some symptoms of metabolic syndrome in Russian adults with obesity and hypertension. Methods In this 3-week, randomized, double-blind, placebo-controlled, parallel pilot study, 25 subjects ingested Probiotic Cheese and 15 ingested control Cheese. Fifty grams of each Cheese provided 175 kcal of energy. Blood pressure (BP), anthropometric characteristics, markers of liver and kidney function, metabolic indices (plasma glucose, lipids, and cholesterol), and urine polyamines were measured. Counts of fecal lactobacilli and L . plantarum TENSIA were evaluated using molecular methods. The data were analyzed by t-test for independent samples and Spearman’s partial correlation analysis. Results The Probiotic L. plantarum TENSIA was present in variable amounts (529.6 ± 232.5 gene copies) in 16/25 (64%) study subjects. Body mass index (BMI) was significantly reduced (p = 0.031) in the Probiotic Cheese group versus the control Cheese group. The changes in BMI were closely associated with the water content of the body (r = 0.570, p = 0.0007) when adjusted for sex and age. Higher values of intestinal lactobacilli after Probiotic Cheese consumption were associated with higher BMI (r = 0.383, p = 0.0305) and urinary putrescine content (r = 0.475, p = 0.006). In patients simultaneously treated with BP-lowering drugs, similar reductions of BP were observed in both groups. A positive association was detected between TENSIA colonization and the extent of change of morning diastolic BP (r = 0.617, p = 0.0248) and a trend toward lower values of morning systolic BP (r = −0.527, p = 0.0640) at the end of the study after adjusting for BMI, age, and sex. Conclusion In a pilot study of obese hypertensive patients, a hypocaloric diet supplemented with a Probiotic Cheese helps to reduce BMI and arterial BP values, recognized symptoms of metabolic syndrome. Trial registration Current Controlled Trials ISRCTN76271778
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hypocaloric diet supplemented with Probiotic Cheese improves body mass index and blood pressure indices of obese hypertensive patients a randomized double blind placebo controlled pilot study
Nutrition Journal, 2013Co-Authors: Khaider K. Sharafedtinov, Oksana A. Plotnikova, Ravilay I. Alexeeva, Tatjana B. Sentsova, Epp Songisepp, Jelena Stsepetova, Imbi Smidt, Marika MikelsaarAbstract:Background Gut lactobacilli can affect the metabolic functions of healthy humans. We tested whether a 1500 kcal/d diet supplemented with Cheese containing the Probiotic Lactobacillus plantarum TENSIA (Deutsche Sammlung fur Mikroorganismen, DSM 21380) could reduce some symptoms of metabolic syndrome in Russian adults with obesity and hypertension.
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Hypocaloric diet supplemented with Probiotic Cheese improves body mass index and blood pressure indices of obese hypertensive patients - A randomized double-blind placebo-controlled pilot study
Nutrition Journal, 2013Co-Authors: Khaider K. Sharafedtinov, Oksana A. Plotnikova, Ravilay I. Alexeeva, Tatjana B. Sentsova, Epp Songisepp, Jelena Stsepetova, Imbi Smidt, Marika MikelsaarAbstract:BACKGROUND: Gut lactobacilli can affect the metabolic functions of healthy humans. We tested whether a 1500 kcal/d diet supplemented with Cheese containing the Probiotic Lactobacillus plantarum TENSIA (Deutsche Sammlung für Mikroorganismen, DSM 21380) could reduce some symptoms of metabolic syndrome in Russian adults with obesity and hypertension.\n\nMETHODS: In this 3-week, randomized, double-blind, placebo-controlled, parallel pilot study, 25 subjects ingested Probiotic Cheese and 15 ingested control Cheese. Fifty grams of each Cheese provided 175 kcal of energy. Blood pressure (BP), anthropometric characteristics, markers of liver and kidney function, metabolic indices (plasma glucose, lipids, and cholesterol), and urine polyamines were measured. Counts of fecal lactobacilli and L. plantarum TENSIA were evaluated using molecular methods. The data were analyzed by t-test for independent samples and Spearman's partial correlation analysis.\n\nRESULTS: The Probiotic L. plantarum TENSIA was present in variable amounts (529.6 ± 232.5 gene copies) in 16/25 (64%) study subjects. Body mass index (BMI) was significantly reduced (p = 0.031) in the Probiotic Cheese group versus the control Cheese group. The changes in BMI were closely associated with the water content of the body (r = 0.570, p = 0.0007) when adjusted for sex and age. Higher values of intestinal lactobacilli after Probiotic Cheese consumption were associated with higher BMI (r = 0.383, p = 0.0305) and urinary putrescine content (r = 0.475, p = 0.006). In patients simultaneously treated with BP-lowering drugs, similar reductions of BP were observed in both groups. A positive association was detected between TENSIA colonization and the extent of change of morning diastolic BP (r = 0.617, p = 0.0248) and a trend toward lower values of morning systolic BP (r = -0.527, p = 0.0640) at the end of the study after adjusting for BMI, age, and sex.\n\nCONCLUSION: In a pilot study of obese hypertensive patients, a hypocaloric diet supplemented with a Probiotic Cheese helps to reduce BMI and arterial BP values, recognized symptoms of metabolic syndrome.
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safety of a Probiotic Cheese containing lactobacillus plantarum tensia according to a variety of health indices in different age groups
Journal of Dairy Science, 2012Co-Authors: Epp Songisepp, Jelena Stsepetova, Imbi Smidt, Pirje Hutt, Merle Ratsep, E Shkut, Siiri Koljalg, Kai Truusalu, Helgi Kolk, M ZaguraAbstract:Abstract Safety of the Probiotic Lactobacillus plantarum strain Tensia (DSM 21380) was tested in vitro, in semihard Edam-type Cheese, in an animal model and after consumption of the Probiotic Cheese in double-blind randomized placebo-controlled human intervention studies with different age groups. The susceptibility of L. plantarum Tensia to 8 antibiotics, and the presence of tetracycline ( tet M, S, O, K, L) genes and class 1 integron was assessed by applying epsilometer-test and PCR-based methods. Production of biogenic amines by the Probiotic strain in decarboxylation medium containing 1% of l-histidine, l-glutamine, l-ornithine, l-arginine, or l-lysine and in Cheese was tested by gas chromatography. The biosafety of L. plantarum Tensia was evaluated on National Institutes of Health-line mice fed Cheese containing Tensia at a concentration of 9.6 log cfu/g for 30 consecutive days. In human intervention trials in adults and the elderly, the effects of different doses of Edam-type Cheese and the Probiotic bacterium on BW, gut functionality indices, and host metabolism were evaluated. The strain L. plantarum Tensia was susceptible to all tested antibiotics and did not possess the tetracycline resistance-determining genes tet (L), tet (S) and tet (O), nor did it contain the integron ( Int1 ) gene. However, the strain was tet (K) and tet (M) positive. Lactobacillus plantarum Tensia did not produce potentially harmful biogenic amines, such as histamine or cadaverine. The amount of tyramine produced in the Cheese environment during ripening and after 15 wk of storage was below the clinically significant content. In the animal model, no translocation of the administered strain or other microbes into the blood or organs of mice was detected. No harmful effect was observed on body mass index, inflammatory markers, or serum lipidograms during human intervention trials with different age groups at a daily dose of 10.3 or 8.17 log cfu/serving for 3 wk. No negative effect on gastrointestinal welfare was observed, but the consumption of 100g/d for 3 wk caused hard stools from the second week of the trial. The content of total lactobacilli increased in feces, and the presence of the ingested Probiotic strain was confirmed after the consumption of Cheese. Thus, L. plantarum strain Tensia is suitable for generally recognized as safe (GRAS) and qualified presumption of safety (QPS) criteria because it did not have any undesirable characteristics. The regular semihard Edam-type Cheese (fat content of 26%) with the Probiotic additive at a daily dose of 50g or in excess (100g) and with a Probiotic daily dose of 10 log cfu for 3 wk was safe.
Imbi Smidt - One of the best experts on this subject based on the ideXlab platform.
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Hypocaloric diet supplemented with Probiotic Cheese improves body mass index and blood pressure indices of obese hypertensive patients - a randomized double-blind placebo-controlled pilot study
Nutrition Journal, 2013Co-Authors: Khaider K. Sharafedtinov, Oksana A. Plotnikova, Ravilay I. Alexeeva, Tatjana B. Sentsova, Epp Songisepp, Jelena Stsepetova, Imbi Smidt, Marika MikelsaarAbstract:Background Gut lactobacilli can affect the metabolic functions of healthy humans. We tested whether a 1500 kcal/d diet supplemented with Cheese containing the Probiotic Lactobacillus plantarum TENSIA (Deutsche Sammlung für Mikroorganismen, DSM 21380) could reduce some symptoms of metabolic syndrome in Russian adults with obesity and hypertension. Methods In this 3-week, randomized, double-blind, placebo-controlled, parallel pilot study, 25 subjects ingested Probiotic Cheese and 15 ingested control Cheese. Fifty grams of each Cheese provided 175 kcal of energy. Blood pressure (BP), anthropometric characteristics, markers of liver and kidney function, metabolic indices (plasma glucose, lipids, and cholesterol), and urine polyamines were measured. Counts of fecal lactobacilli and L . plantarum TENSIA were evaluated using molecular methods. The data were analyzed by t-test for independent samples and Spearman’s partial correlation analysis. Results The Probiotic L. plantarum TENSIA was present in variable amounts (529.6 ± 232.5 gene copies) in 16/25 (64%) study subjects. Body mass index (BMI) was significantly reduced (p = 0.031) in the Probiotic Cheese group versus the control Cheese group. The changes in BMI were closely associated with the water content of the body (r = 0.570, p = 0.0007) when adjusted for sex and age. Higher values of intestinal lactobacilli after Probiotic Cheese consumption were associated with higher BMI (r = 0.383, p = 0.0305) and urinary putrescine content (r = 0.475, p = 0.006). In patients simultaneously treated with BP-lowering drugs, similar reductions of BP were observed in both groups. A positive association was detected between TENSIA colonization and the extent of change of morning diastolic BP (r = 0.617, p = 0.0248) and a trend toward lower values of morning systolic BP (r = −0.527, p = 0.0640) at the end of the study after adjusting for BMI, age, and sex. Conclusion In a pilot study of obese hypertensive patients, a hypocaloric diet supplemented with a Probiotic Cheese helps to reduce BMI and arterial BP values, recognized symptoms of metabolic syndrome. Trial registration Current Controlled Trials ISRCTN76271778
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hypocaloric diet supplemented with Probiotic Cheese improves body mass index and blood pressure indices of obese hypertensive patients a randomized double blind placebo controlled pilot study
Nutrition Journal, 2013Co-Authors: Khaider K. Sharafedtinov, Oksana A. Plotnikova, Ravilay I. Alexeeva, Tatjana B. Sentsova, Epp Songisepp, Jelena Stsepetova, Imbi Smidt, Marika MikelsaarAbstract:Background Gut lactobacilli can affect the metabolic functions of healthy humans. We tested whether a 1500 kcal/d diet supplemented with Cheese containing the Probiotic Lactobacillus plantarum TENSIA (Deutsche Sammlung fur Mikroorganismen, DSM 21380) could reduce some symptoms of metabolic syndrome in Russian adults with obesity and hypertension.
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Hypocaloric diet supplemented with Probiotic Cheese improves body mass index and blood pressure indices of obese hypertensive patients - A randomized double-blind placebo-controlled pilot study
Nutrition Journal, 2013Co-Authors: Khaider K. Sharafedtinov, Oksana A. Plotnikova, Ravilay I. Alexeeva, Tatjana B. Sentsova, Epp Songisepp, Jelena Stsepetova, Imbi Smidt, Marika MikelsaarAbstract:BACKGROUND: Gut lactobacilli can affect the metabolic functions of healthy humans. We tested whether a 1500 kcal/d diet supplemented with Cheese containing the Probiotic Lactobacillus plantarum TENSIA (Deutsche Sammlung für Mikroorganismen, DSM 21380) could reduce some symptoms of metabolic syndrome in Russian adults with obesity and hypertension.\n\nMETHODS: In this 3-week, randomized, double-blind, placebo-controlled, parallel pilot study, 25 subjects ingested Probiotic Cheese and 15 ingested control Cheese. Fifty grams of each Cheese provided 175 kcal of energy. Blood pressure (BP), anthropometric characteristics, markers of liver and kidney function, metabolic indices (plasma glucose, lipids, and cholesterol), and urine polyamines were measured. Counts of fecal lactobacilli and L. plantarum TENSIA were evaluated using molecular methods. The data were analyzed by t-test for independent samples and Spearman's partial correlation analysis.\n\nRESULTS: The Probiotic L. plantarum TENSIA was present in variable amounts (529.6 ± 232.5 gene copies) in 16/25 (64%) study subjects. Body mass index (BMI) was significantly reduced (p = 0.031) in the Probiotic Cheese group versus the control Cheese group. The changes in BMI were closely associated with the water content of the body (r = 0.570, p = 0.0007) when adjusted for sex and age. Higher values of intestinal lactobacilli after Probiotic Cheese consumption were associated with higher BMI (r = 0.383, p = 0.0305) and urinary putrescine content (r = 0.475, p = 0.006). In patients simultaneously treated with BP-lowering drugs, similar reductions of BP were observed in both groups. A positive association was detected between TENSIA colonization and the extent of change of morning diastolic BP (r = 0.617, p = 0.0248) and a trend toward lower values of morning systolic BP (r = -0.527, p = 0.0640) at the end of the study after adjusting for BMI, age, and sex.\n\nCONCLUSION: In a pilot study of obese hypertensive patients, a hypocaloric diet supplemented with a Probiotic Cheese helps to reduce BMI and arterial BP values, recognized symptoms of metabolic syndrome.
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safety of a Probiotic Cheese containing lactobacillus plantarum tensia according to a variety of health indices in different age groups
Journal of Dairy Science, 2012Co-Authors: Epp Songisepp, Jelena Stsepetova, Imbi Smidt, Pirje Hutt, Merle Ratsep, E Shkut, Siiri Koljalg, Kai Truusalu, Helgi Kolk, M ZaguraAbstract:Abstract Safety of the Probiotic Lactobacillus plantarum strain Tensia (DSM 21380) was tested in vitro, in semihard Edam-type Cheese, in an animal model and after consumption of the Probiotic Cheese in double-blind randomized placebo-controlled human intervention studies with different age groups. The susceptibility of L. plantarum Tensia to 8 antibiotics, and the presence of tetracycline ( tet M, S, O, K, L) genes and class 1 integron was assessed by applying epsilometer-test and PCR-based methods. Production of biogenic amines by the Probiotic strain in decarboxylation medium containing 1% of l-histidine, l-glutamine, l-ornithine, l-arginine, or l-lysine and in Cheese was tested by gas chromatography. The biosafety of L. plantarum Tensia was evaluated on National Institutes of Health-line mice fed Cheese containing Tensia at a concentration of 9.6 log cfu/g for 30 consecutive days. In human intervention trials in adults and the elderly, the effects of different doses of Edam-type Cheese and the Probiotic bacterium on BW, gut functionality indices, and host metabolism were evaluated. The strain L. plantarum Tensia was susceptible to all tested antibiotics and did not possess the tetracycline resistance-determining genes tet (L), tet (S) and tet (O), nor did it contain the integron ( Int1 ) gene. However, the strain was tet (K) and tet (M) positive. Lactobacillus plantarum Tensia did not produce potentially harmful biogenic amines, such as histamine or cadaverine. The amount of tyramine produced in the Cheese environment during ripening and after 15 wk of storage was below the clinically significant content. In the animal model, no translocation of the administered strain or other microbes into the blood or organs of mice was detected. No harmful effect was observed on body mass index, inflammatory markers, or serum lipidograms during human intervention trials with different age groups at a daily dose of 10.3 or 8.17 log cfu/serving for 3 wk. No negative effect on gastrointestinal welfare was observed, but the consumption of 100g/d for 3 wk caused hard stools from the second week of the trial. The content of total lactobacilli increased in feces, and the presence of the ingested Probiotic strain was confirmed after the consumption of Cheese. Thus, L. plantarum strain Tensia is suitable for generally recognized as safe (GRAS) and qualified presumption of safety (QPS) criteria because it did not have any undesirable characteristics. The regular semihard Edam-type Cheese (fat content of 26%) with the Probiotic additive at a daily dose of 50g or in excess (100g) and with a Probiotic daily dose of 10 log cfu for 3 wk was safe.
Jelena Stsepetova - One of the best experts on this subject based on the ideXlab platform.
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Hypocaloric diet supplemented with Probiotic Cheese improves body mass index and blood pressure indices of obese hypertensive patients - a randomized double-blind placebo-controlled pilot study
Nutrition Journal, 2013Co-Authors: Khaider K. Sharafedtinov, Oksana A. Plotnikova, Ravilay I. Alexeeva, Tatjana B. Sentsova, Epp Songisepp, Jelena Stsepetova, Imbi Smidt, Marika MikelsaarAbstract:Background Gut lactobacilli can affect the metabolic functions of healthy humans. We tested whether a 1500 kcal/d diet supplemented with Cheese containing the Probiotic Lactobacillus plantarum TENSIA (Deutsche Sammlung für Mikroorganismen, DSM 21380) could reduce some symptoms of metabolic syndrome in Russian adults with obesity and hypertension. Methods In this 3-week, randomized, double-blind, placebo-controlled, parallel pilot study, 25 subjects ingested Probiotic Cheese and 15 ingested control Cheese. Fifty grams of each Cheese provided 175 kcal of energy. Blood pressure (BP), anthropometric characteristics, markers of liver and kidney function, metabolic indices (plasma glucose, lipids, and cholesterol), and urine polyamines were measured. Counts of fecal lactobacilli and L . plantarum TENSIA were evaluated using molecular methods. The data were analyzed by t-test for independent samples and Spearman’s partial correlation analysis. Results The Probiotic L. plantarum TENSIA was present in variable amounts (529.6 ± 232.5 gene copies) in 16/25 (64%) study subjects. Body mass index (BMI) was significantly reduced (p = 0.031) in the Probiotic Cheese group versus the control Cheese group. The changes in BMI were closely associated with the water content of the body (r = 0.570, p = 0.0007) when adjusted for sex and age. Higher values of intestinal lactobacilli after Probiotic Cheese consumption were associated with higher BMI (r = 0.383, p = 0.0305) and urinary putrescine content (r = 0.475, p = 0.006). In patients simultaneously treated with BP-lowering drugs, similar reductions of BP were observed in both groups. A positive association was detected between TENSIA colonization and the extent of change of morning diastolic BP (r = 0.617, p = 0.0248) and a trend toward lower values of morning systolic BP (r = −0.527, p = 0.0640) at the end of the study after adjusting for BMI, age, and sex. Conclusion In a pilot study of obese hypertensive patients, a hypocaloric diet supplemented with a Probiotic Cheese helps to reduce BMI and arterial BP values, recognized symptoms of metabolic syndrome. Trial registration Current Controlled Trials ISRCTN76271778
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hypocaloric diet supplemented with Probiotic Cheese improves body mass index and blood pressure indices of obese hypertensive patients a randomized double blind placebo controlled pilot study
Nutrition Journal, 2013Co-Authors: Khaider K. Sharafedtinov, Oksana A. Plotnikova, Ravilay I. Alexeeva, Tatjana B. Sentsova, Epp Songisepp, Jelena Stsepetova, Imbi Smidt, Marika MikelsaarAbstract:Background Gut lactobacilli can affect the metabolic functions of healthy humans. We tested whether a 1500 kcal/d diet supplemented with Cheese containing the Probiotic Lactobacillus plantarum TENSIA (Deutsche Sammlung fur Mikroorganismen, DSM 21380) could reduce some symptoms of metabolic syndrome in Russian adults with obesity and hypertension.
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Hypocaloric diet supplemented with Probiotic Cheese improves body mass index and blood pressure indices of obese hypertensive patients - A randomized double-blind placebo-controlled pilot study
Nutrition Journal, 2013Co-Authors: Khaider K. Sharafedtinov, Oksana A. Plotnikova, Ravilay I. Alexeeva, Tatjana B. Sentsova, Epp Songisepp, Jelena Stsepetova, Imbi Smidt, Marika MikelsaarAbstract:BACKGROUND: Gut lactobacilli can affect the metabolic functions of healthy humans. We tested whether a 1500 kcal/d diet supplemented with Cheese containing the Probiotic Lactobacillus plantarum TENSIA (Deutsche Sammlung für Mikroorganismen, DSM 21380) could reduce some symptoms of metabolic syndrome in Russian adults with obesity and hypertension.\n\nMETHODS: In this 3-week, randomized, double-blind, placebo-controlled, parallel pilot study, 25 subjects ingested Probiotic Cheese and 15 ingested control Cheese. Fifty grams of each Cheese provided 175 kcal of energy. Blood pressure (BP), anthropometric characteristics, markers of liver and kidney function, metabolic indices (plasma glucose, lipids, and cholesterol), and urine polyamines were measured. Counts of fecal lactobacilli and L. plantarum TENSIA were evaluated using molecular methods. The data were analyzed by t-test for independent samples and Spearman's partial correlation analysis.\n\nRESULTS: The Probiotic L. plantarum TENSIA was present in variable amounts (529.6 ± 232.5 gene copies) in 16/25 (64%) study subjects. Body mass index (BMI) was significantly reduced (p = 0.031) in the Probiotic Cheese group versus the control Cheese group. The changes in BMI were closely associated with the water content of the body (r = 0.570, p = 0.0007) when adjusted for sex and age. Higher values of intestinal lactobacilli after Probiotic Cheese consumption were associated with higher BMI (r = 0.383, p = 0.0305) and urinary putrescine content (r = 0.475, p = 0.006). In patients simultaneously treated with BP-lowering drugs, similar reductions of BP were observed in both groups. A positive association was detected between TENSIA colonization and the extent of change of morning diastolic BP (r = 0.617, p = 0.0248) and a trend toward lower values of morning systolic BP (r = -0.527, p = 0.0640) at the end of the study after adjusting for BMI, age, and sex.\n\nCONCLUSION: In a pilot study of obese hypertensive patients, a hypocaloric diet supplemented with a Probiotic Cheese helps to reduce BMI and arterial BP values, recognized symptoms of metabolic syndrome.
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safety of a Probiotic Cheese containing lactobacillus plantarum tensia according to a variety of health indices in different age groups
Journal of Dairy Science, 2012Co-Authors: Epp Songisepp, Jelena Stsepetova, Imbi Smidt, Pirje Hutt, Merle Ratsep, E Shkut, Siiri Koljalg, Kai Truusalu, Helgi Kolk, M ZaguraAbstract:Abstract Safety of the Probiotic Lactobacillus plantarum strain Tensia (DSM 21380) was tested in vitro, in semihard Edam-type Cheese, in an animal model and after consumption of the Probiotic Cheese in double-blind randomized placebo-controlled human intervention studies with different age groups. The susceptibility of L. plantarum Tensia to 8 antibiotics, and the presence of tetracycline ( tet M, S, O, K, L) genes and class 1 integron was assessed by applying epsilometer-test and PCR-based methods. Production of biogenic amines by the Probiotic strain in decarboxylation medium containing 1% of l-histidine, l-glutamine, l-ornithine, l-arginine, or l-lysine and in Cheese was tested by gas chromatography. The biosafety of L. plantarum Tensia was evaluated on National Institutes of Health-line mice fed Cheese containing Tensia at a concentration of 9.6 log cfu/g for 30 consecutive days. In human intervention trials in adults and the elderly, the effects of different doses of Edam-type Cheese and the Probiotic bacterium on BW, gut functionality indices, and host metabolism were evaluated. The strain L. plantarum Tensia was susceptible to all tested antibiotics and did not possess the tetracycline resistance-determining genes tet (L), tet (S) and tet (O), nor did it contain the integron ( Int1 ) gene. However, the strain was tet (K) and tet (M) positive. Lactobacillus plantarum Tensia did not produce potentially harmful biogenic amines, such as histamine or cadaverine. The amount of tyramine produced in the Cheese environment during ripening and after 15 wk of storage was below the clinically significant content. In the animal model, no translocation of the administered strain or other microbes into the blood or organs of mice was detected. No harmful effect was observed on body mass index, inflammatory markers, or serum lipidograms during human intervention trials with different age groups at a daily dose of 10.3 or 8.17 log cfu/serving for 3 wk. No negative effect on gastrointestinal welfare was observed, but the consumption of 100g/d for 3 wk caused hard stools from the second week of the trial. The content of total lactobacilli increased in feces, and the presence of the ingested Probiotic strain was confirmed after the consumption of Cheese. Thus, L. plantarum strain Tensia is suitable for generally recognized as safe (GRAS) and qualified presumption of safety (QPS) criteria because it did not have any undesirable characteristics. The regular semihard Edam-type Cheese (fat content of 26%) with the Probiotic additive at a daily dose of 50g or in excess (100g) and with a Probiotic daily dose of 10 log cfu for 3 wk was safe.
Khaider K. Sharafedtinov - One of the best experts on this subject based on the ideXlab platform.
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hypocaloric diet supplemented with Probiotic Cheese improves body mass index and blood pressure indices of obese hypertensive patients a randomized double blind placebo controlled pilot study
Nutrition Journal, 2013Co-Authors: Khaider K. Sharafedtinov, Oksana A. Plotnikova, Ravilay I. Alexeeva, Tatjana B. Sentsova, Epp Songisepp, Jelena Stsepetova, Imbi Smidt, Marika MikelsaarAbstract:Background Gut lactobacilli can affect the metabolic functions of healthy humans. We tested whether a 1500 kcal/d diet supplemented with Cheese containing the Probiotic Lactobacillus plantarum TENSIA (Deutsche Sammlung fur Mikroorganismen, DSM 21380) could reduce some symptoms of metabolic syndrome in Russian adults with obesity and hypertension.
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Hypocaloric diet supplemented with Probiotic Cheese improves body mass index and blood pressure indices of obese hypertensive patients - a randomized double-blind placebo-controlled pilot study
Nutrition Journal, 2013Co-Authors: Khaider K. Sharafedtinov, Oksana A. Plotnikova, Ravilay I. Alexeeva, Tatjana B. Sentsova, Epp Songisepp, Jelena Stsepetova, Imbi Smidt, Marika MikelsaarAbstract:Background Gut lactobacilli can affect the metabolic functions of healthy humans. We tested whether a 1500 kcal/d diet supplemented with Cheese containing the Probiotic Lactobacillus plantarum TENSIA (Deutsche Sammlung für Mikroorganismen, DSM 21380) could reduce some symptoms of metabolic syndrome in Russian adults with obesity and hypertension. Methods In this 3-week, randomized, double-blind, placebo-controlled, parallel pilot study, 25 subjects ingested Probiotic Cheese and 15 ingested control Cheese. Fifty grams of each Cheese provided 175 kcal of energy. Blood pressure (BP), anthropometric characteristics, markers of liver and kidney function, metabolic indices (plasma glucose, lipids, and cholesterol), and urine polyamines were measured. Counts of fecal lactobacilli and L . plantarum TENSIA were evaluated using molecular methods. The data were analyzed by t-test for independent samples and Spearman’s partial correlation analysis. Results The Probiotic L. plantarum TENSIA was present in variable amounts (529.6 ± 232.5 gene copies) in 16/25 (64%) study subjects. Body mass index (BMI) was significantly reduced (p = 0.031) in the Probiotic Cheese group versus the control Cheese group. The changes in BMI were closely associated with the water content of the body (r = 0.570, p = 0.0007) when adjusted for sex and age. Higher values of intestinal lactobacilli after Probiotic Cheese consumption were associated with higher BMI (r = 0.383, p = 0.0305) and urinary putrescine content (r = 0.475, p = 0.006). In patients simultaneously treated with BP-lowering drugs, similar reductions of BP were observed in both groups. A positive association was detected between TENSIA colonization and the extent of change of morning diastolic BP (r = 0.617, p = 0.0248) and a trend toward lower values of morning systolic BP (r = −0.527, p = 0.0640) at the end of the study after adjusting for BMI, age, and sex. Conclusion In a pilot study of obese hypertensive patients, a hypocaloric diet supplemented with a Probiotic Cheese helps to reduce BMI and arterial BP values, recognized symptoms of metabolic syndrome. Trial registration Current Controlled Trials ISRCTN76271778
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Hypocaloric diet supplemented with Probiotic Cheese improves body mass index and blood pressure indices of obese hypertensive patients - A randomized double-blind placebo-controlled pilot study
Nutrition Journal, 2013Co-Authors: Khaider K. Sharafedtinov, Oksana A. Plotnikova, Ravilay I. Alexeeva, Tatjana B. Sentsova, Epp Songisepp, Jelena Stsepetova, Imbi Smidt, Marika MikelsaarAbstract:BACKGROUND: Gut lactobacilli can affect the metabolic functions of healthy humans. We tested whether a 1500 kcal/d diet supplemented with Cheese containing the Probiotic Lactobacillus plantarum TENSIA (Deutsche Sammlung für Mikroorganismen, DSM 21380) could reduce some symptoms of metabolic syndrome in Russian adults with obesity and hypertension.\n\nMETHODS: In this 3-week, randomized, double-blind, placebo-controlled, parallel pilot study, 25 subjects ingested Probiotic Cheese and 15 ingested control Cheese. Fifty grams of each Cheese provided 175 kcal of energy. Blood pressure (BP), anthropometric characteristics, markers of liver and kidney function, metabolic indices (plasma glucose, lipids, and cholesterol), and urine polyamines were measured. Counts of fecal lactobacilli and L. plantarum TENSIA were evaluated using molecular methods. The data were analyzed by t-test for independent samples and Spearman's partial correlation analysis.\n\nRESULTS: The Probiotic L. plantarum TENSIA was present in variable amounts (529.6 ± 232.5 gene copies) in 16/25 (64%) study subjects. Body mass index (BMI) was significantly reduced (p = 0.031) in the Probiotic Cheese group versus the control Cheese group. The changes in BMI were closely associated with the water content of the body (r = 0.570, p = 0.0007) when adjusted for sex and age. Higher values of intestinal lactobacilli after Probiotic Cheese consumption were associated with higher BMI (r = 0.383, p = 0.0305) and urinary putrescine content (r = 0.475, p = 0.006). In patients simultaneously treated with BP-lowering drugs, similar reductions of BP were observed in both groups. A positive association was detected between TENSIA colonization and the extent of change of morning diastolic BP (r = 0.617, p = 0.0248) and a trend toward lower values of morning systolic BP (r = -0.527, p = 0.0640) at the end of the study after adjusting for BMI, age, and sex.\n\nCONCLUSION: In a pilot study of obese hypertensive patients, a hypocaloric diet supplemented with a Probiotic Cheese helps to reduce BMI and arterial BP values, recognized symptoms of metabolic syndrome.