The Experts below are selected from a list of 42006 Experts worldwide ranked by ideXlab platform

Raha Abdul Rahim - One of the best experts on this subject based on the ideXlab platform.

  • a review on lactococcus lactis from food to factory
    Microbial Cell Factories, 2017
    Co-Authors: Adelene Ailian Song, Sweehua Erin Lim, Raha Abdul Rahim
    Abstract:

    Lactococcus lactis has progressed a long way since its discovery and initial use in dairy Product Fermentation, to its present biotechnological applications in genetic engineering for the Production of various recombinant proteins and metabolites that transcends the heterologous species barrier. Key desirable features of this gram-positive lactic acid non-colonizing gut bacteria include its generally recognized as safe (GRAS) status, probiotic properties, the absence of inclusion bodies and endotoxins, surface display and extracellular secretion technology, and a diverse selection of cloning and inducible expression vectors. This have made L. lactis a desirable and promising host on par with other well established model bacterial or yeast systems such as Escherichia coli, Salmonella cerevisiae and Bacillus subtilis. In this article, we review recent technological advancements, challenges, future prospects and current diversified examples on the use of L. lactis as a microbial cell factory. Additionally, we will also highlight latest medical-based applications involving whole-cell L. lactis as a live delivery vector for the administration of therapeutics against both communicable and non-communicable diseases.

Joshi Lokesh - One of the best experts on this subject based on the ideXlab platform.

  • Identification of putative adhesins and carbohydrate ligands of Lactobacillus paracasei using a combinatorial in silico and glycomics microarray profiling approach
    Oxford University Press (OUP), 2020
    Co-Authors: Houeix Benoit, Synowsky Silvia, Cairns, Michael T., Kane Marian, Kilcoyne Michelle, Joshi Lokesh
    Abstract:

    Commensal bacteria must colonize host mucosal surfaces to exert health-promoting properties, and bind to gastrointestinal tract (GIT) mucins via their cell surface adhesins. Considerable effort has been directed towards discovery of pathogen adhesins and their ligands to develop anti-infective strategies; however, little is known about the lectin-like adhesins and associated carbohydrate ligands in commensals. In this study, an in silico approach was used to detect surface exposed adhesins in the human commensal Lactobacillus paracasei subsp. paracasei, a promising probiotic commonly used in dairy Product Fermentation that presents anti-microbial activity. Of the 13 adhesin candidates, 3 sortase-dependent pili clusters were identified in this strain and expression of the adhesin candidate genes was confirmed in vitro. Mass spectrometry analysis confirmed the presence of surface adhesin elongation factor Tu and the chaperonin GroEL, but not pili expression. Whole cells were subsequently incubated on microarrays featuring a panel of GIT mucins from nine different mammalian species and two human-derived cell lines and a library of carbohydrate structures. Binding profiles were compared to those of two known pili-producing lactobacilli, L. johnsonii and L. rhamnosus and all Lactobacillus species displayed overlapping but distinct signatures, which may indicate different abilities for regiospecific GIT colonization. In addition, L. paracasei whole cells favoured binding to ¿-(2 ¿ 3)-linked sialic acid and ¿-(1 ¿ 2)-linked fucose-containing carbohydrate structures including blood groups A, B and O and Lewis antigens x, y and b. This study furthers our understanding of host-commensal cross-talk by identifying potential adhesins and specific GIT mucin and carbohydrate ligands and provides insight into the selection of colonization sites by commensals in the GIT.This work was supported by Science Foundation Ireland Strategic Research Cluster programme in support of Alimentary Glycoscience Research Cluster (grant number 08/SRC/B1393). MK is grateful to the Royal Society of Chemistry Analytical Chemistry Trust Fund (ACTF) for the ACTF Fellowship Award 2018. The authors acknowledge the Centre for Microscopy & Imaging at the National University of Ireland, Galway (NUI, Galway) funded by NUI Galway and the Irish Government Programme for Research in Third Level Institutions, Cycles 4 and 5, National Development Plan 2007-2013. The authors thank Prof. Maarten Van de Guchte and Mr Valentin Loux, Unité Mathématique, Informatique et Génome, INRA, Jouy en Josas, France for help with SurfG+ analysis, Prof. Y. C. Lee, Department of Biology, Johns Hopkins University, Baltimore, Maryland, U.S.A., for the kind gift of pigeon egg white and Prof. Stephen D. Carrington, Dr. Mary E. Gallagher, Dr. Colm Reid and Dr. Marguerite Clyne of University College Dublin for the kind gift of mucins.2020-11-1

  • Identification of putative adhesins and carbohydrate ligands of Lactobacillus paracasei using a combinatorial in silico and glycomics microarray profiling approach
    'Oxford University Press (OUP)', 2020
    Co-Authors: Houeix Benoit, Synowsky Silvia, Cairns, Michael T., Kane Marian, Kilcoyne Michelle, Joshi Lokesh
    Abstract:

    Commensal bacteria must colonize host mucosal surfaces to exert health-promoting properties, and bind to gastrointestinal tract (GIT) mucins via their cell surface adhesins. Considerable effort has been directed towards discovery of pathogen adhesins and their ligands to develop anti-infective strategies; however, little is known about the lectin-like adhesins and associated carbohydrate ligands in commensals. In this study, an in silico approach was used to detect surface exposed adhesins in the human commensal Lactobacillus paracasei subsp. paracasei, a promising probiotic commonly used in dairy Product Fermentation that presents anti-microbial activity. Of the 13 adhesin candidates, 3 sortase-dependent pili clusters were identified in this strain and expression of the adhesin candidate genes was confirmed in vitro. Mass spectrometry analysis confirmed the presence of surface adhesin elongation factor Tu and the chaperonin GroEL, but not pili expression. Whole cells were subsequently incubated on microarrays featuring a panel of GIT mucins from nine different mammalian species and two human-derived cell lines and a library of carbohydrate structures. Binding profiles were compared to those of two known pili-producing lactobacilli, L. johnsonii and L. rhamnosus and all Lactobacillus species displayed overlapping but distinct signatures, which may indicate different abilities for regiospecific GIT colonization. In addition, L. paracasei whole cells favoured binding to ¿-(2 ¿ 3)-linked sialic acid and ¿-(1 ¿ 2)-linked fucose-containing carbohydrate structures including blood groups A, B and O and Lewis antigens x, y and b. This study furthers our understanding of host-commensal cross-talk by identifying potential adhesins and specific GIT mucin and carbohydrate ligands and provides insight into the selection of colonization sites by commensals in the GIT.This work was supported by Science Foundation Ireland Strategic Research Cluster programme in support of Alimentary Glycoscience Research Cluster (grant number 08/SRC/B1393). MK is grateful to the Royal Society of Chemistry Analytical Chemistry Trust Fund (ACTF) for the ACTF Fellowship Award 2018. The authors acknowledge the Centre for Microscopy & Imaging at the National University of Ireland, Galway (NUI, Galway) funded by NUI Galway and the Irish Government Programme for Research in Third Level Institutions, Cycles 4 and 5, National Development Plan 2007-2013. The authors thank Prof. Maarten Van de Guchte and Mr Valentin Loux, Unité Mathématique, Informatique et Génome, INRA, Jouy en Josas, France for help with SurfG+ analysis, Prof. Y. C. Lee, Department of Biology, Johns Hopkins University, Baltimore, Maryland, U.S.A., for the kind gift of pigeon egg white and Prof. Stephen D. Carrington, Dr. Mary E. Gallagher, Dr. Colm Reid and Dr. Marguerite Clyne of University College Dublin for the kind gift of mucins.peer-reviewed2020-11-1

Duolong Zhu - One of the best experts on this subject based on the ideXlab platform.

  • enhanced heterologous protein Productivity by genome reduction in lactococcus lactis nz9000
    Microbial Cell Factories, 2017
    Co-Authors: Duolong Zhu, Fulu Liu, Per E J Saris, Mingqiang Qiao
    Abstract:

    The implementation of novel chassis organisms to be used as microbial cell factories in industrial applications is an intensive research field. Lactococcus lactis, which is one of the most extensively studied model organisms, exhibits superior ability to be used as engineered host for Fermentation of desirable Products. However, few studies have reported about genome reduction of L. lactis as a clean background for functional genomic studies and a model chassis for desirable Product Fermentation. Four large nonessential DNA regions accounting for 2.83% in L. lactis NZ9000 (L. lactis 9 k) genome (2,530,294 bp) were deleted using the Cre-loxP deletion system as the first steps toward a minimized genome in this study. The mutants were compared with the parental strain in several physiological traits and evaluated as microbial cell factories for heterologous protein Production (intracellular and secretory expression) with the red fluorescent protein (RFP) and the bacteriocin leucocin C (LecC) as reporters. The four mutants grew faster, yielded enhanced biomass, achieved increased adenosine triphosphate content, and diminished maintenance demands compared with the wild strain in the two media tested. In particular, L. lactis 9 k-4 with the largest deletion was identified as the optimum candidate host for recombinant protein Production. With nisin induction, not only the transcriptional efficiency but also the Production levels of the expressed reporters were approximately three- to fourfold improved compared with the wild strain. The expression of lecC gene controlled with strong constitutive promoters P5 and P8 in L. lactis 9 k-4 was also improved significantly. The genome-streamlined L. lactis 9 k-4 outcompeted the parental strain in several physiological traits assessed. Moreover, L. lactis 9 k-4 exhibited good properties as platform organism for protein Production. In future works, the genome of L. lactis will be maximally reduced by using our specific design to provide an even more clean background for functional genomics studies than L. lactis 9 k-4 constructed in this study. Furthermore, an improved background will be potentially available for use in biotechology.

Adelene Ailian Song - One of the best experts on this subject based on the ideXlab platform.

  • a review on lactococcus lactis from food to factory
    Microbial Cell Factories, 2017
    Co-Authors: Adelene Ailian Song, Sweehua Erin Lim, Raha Abdul Rahim
    Abstract:

    Lactococcus lactis has progressed a long way since its discovery and initial use in dairy Product Fermentation, to its present biotechnological applications in genetic engineering for the Production of various recombinant proteins and metabolites that transcends the heterologous species barrier. Key desirable features of this gram-positive lactic acid non-colonizing gut bacteria include its generally recognized as safe (GRAS) status, probiotic properties, the absence of inclusion bodies and endotoxins, surface display and extracellular secretion technology, and a diverse selection of cloning and inducible expression vectors. This have made L. lactis a desirable and promising host on par with other well established model bacterial or yeast systems such as Escherichia coli, Salmonella cerevisiae and Bacillus subtilis. In this article, we review recent technological advancements, challenges, future prospects and current diversified examples on the use of L. lactis as a microbial cell factory. Additionally, we will also highlight latest medical-based applications involving whole-cell L. lactis as a live delivery vector for the administration of therapeutics against both communicable and non-communicable diseases.

Houeix Benoit - One of the best experts on this subject based on the ideXlab platform.

  • Identification of putative adhesins and carbohydrate ligands of Lactobacillus paracasei using a combinatorial in silico and glycomics microarray profiling approach
    Oxford University Press (OUP), 2020
    Co-Authors: Houeix Benoit, Synowsky Silvia, Cairns, Michael T., Kane Marian, Kilcoyne Michelle, Joshi Lokesh
    Abstract:

    Commensal bacteria must colonize host mucosal surfaces to exert health-promoting properties, and bind to gastrointestinal tract (GIT) mucins via their cell surface adhesins. Considerable effort has been directed towards discovery of pathogen adhesins and their ligands to develop anti-infective strategies; however, little is known about the lectin-like adhesins and associated carbohydrate ligands in commensals. In this study, an in silico approach was used to detect surface exposed adhesins in the human commensal Lactobacillus paracasei subsp. paracasei, a promising probiotic commonly used in dairy Product Fermentation that presents anti-microbial activity. Of the 13 adhesin candidates, 3 sortase-dependent pili clusters were identified in this strain and expression of the adhesin candidate genes was confirmed in vitro. Mass spectrometry analysis confirmed the presence of surface adhesin elongation factor Tu and the chaperonin GroEL, but not pili expression. Whole cells were subsequently incubated on microarrays featuring a panel of GIT mucins from nine different mammalian species and two human-derived cell lines and a library of carbohydrate structures. Binding profiles were compared to those of two known pili-producing lactobacilli, L. johnsonii and L. rhamnosus and all Lactobacillus species displayed overlapping but distinct signatures, which may indicate different abilities for regiospecific GIT colonization. In addition, L. paracasei whole cells favoured binding to ¿-(2 ¿ 3)-linked sialic acid and ¿-(1 ¿ 2)-linked fucose-containing carbohydrate structures including blood groups A, B and O and Lewis antigens x, y and b. This study furthers our understanding of host-commensal cross-talk by identifying potential adhesins and specific GIT mucin and carbohydrate ligands and provides insight into the selection of colonization sites by commensals in the GIT.This work was supported by Science Foundation Ireland Strategic Research Cluster programme in support of Alimentary Glycoscience Research Cluster (grant number 08/SRC/B1393). MK is grateful to the Royal Society of Chemistry Analytical Chemistry Trust Fund (ACTF) for the ACTF Fellowship Award 2018. The authors acknowledge the Centre for Microscopy & Imaging at the National University of Ireland, Galway (NUI, Galway) funded by NUI Galway and the Irish Government Programme for Research in Third Level Institutions, Cycles 4 and 5, National Development Plan 2007-2013. The authors thank Prof. Maarten Van de Guchte and Mr Valentin Loux, Unité Mathématique, Informatique et Génome, INRA, Jouy en Josas, France for help with SurfG+ analysis, Prof. Y. C. Lee, Department of Biology, Johns Hopkins University, Baltimore, Maryland, U.S.A., for the kind gift of pigeon egg white and Prof. Stephen D. Carrington, Dr. Mary E. Gallagher, Dr. Colm Reid and Dr. Marguerite Clyne of University College Dublin for the kind gift of mucins.2020-11-1

  • Identification of putative adhesins and carbohydrate ligands of Lactobacillus paracasei using a combinatorial in silico and glycomics microarray profiling approach
    'Oxford University Press (OUP)', 2020
    Co-Authors: Houeix Benoit, Synowsky Silvia, Cairns, Michael T., Kane Marian, Kilcoyne Michelle, Joshi Lokesh
    Abstract:

    Commensal bacteria must colonize host mucosal surfaces to exert health-promoting properties, and bind to gastrointestinal tract (GIT) mucins via their cell surface adhesins. Considerable effort has been directed towards discovery of pathogen adhesins and their ligands to develop anti-infective strategies; however, little is known about the lectin-like adhesins and associated carbohydrate ligands in commensals. In this study, an in silico approach was used to detect surface exposed adhesins in the human commensal Lactobacillus paracasei subsp. paracasei, a promising probiotic commonly used in dairy Product Fermentation that presents anti-microbial activity. Of the 13 adhesin candidates, 3 sortase-dependent pili clusters were identified in this strain and expression of the adhesin candidate genes was confirmed in vitro. Mass spectrometry analysis confirmed the presence of surface adhesin elongation factor Tu and the chaperonin GroEL, but not pili expression. Whole cells were subsequently incubated on microarrays featuring a panel of GIT mucins from nine different mammalian species and two human-derived cell lines and a library of carbohydrate structures. Binding profiles were compared to those of two known pili-producing lactobacilli, L. johnsonii and L. rhamnosus and all Lactobacillus species displayed overlapping but distinct signatures, which may indicate different abilities for regiospecific GIT colonization. In addition, L. paracasei whole cells favoured binding to ¿-(2 ¿ 3)-linked sialic acid and ¿-(1 ¿ 2)-linked fucose-containing carbohydrate structures including blood groups A, B and O and Lewis antigens x, y and b. This study furthers our understanding of host-commensal cross-talk by identifying potential adhesins and specific GIT mucin and carbohydrate ligands and provides insight into the selection of colonization sites by commensals in the GIT.This work was supported by Science Foundation Ireland Strategic Research Cluster programme in support of Alimentary Glycoscience Research Cluster (grant number 08/SRC/B1393). MK is grateful to the Royal Society of Chemistry Analytical Chemistry Trust Fund (ACTF) for the ACTF Fellowship Award 2018. The authors acknowledge the Centre for Microscopy & Imaging at the National University of Ireland, Galway (NUI, Galway) funded by NUI Galway and the Irish Government Programme for Research in Third Level Institutions, Cycles 4 and 5, National Development Plan 2007-2013. The authors thank Prof. Maarten Van de Guchte and Mr Valentin Loux, Unité Mathématique, Informatique et Génome, INRA, Jouy en Josas, France for help with SurfG+ analysis, Prof. Y. C. Lee, Department of Biology, Johns Hopkins University, Baltimore, Maryland, U.S.A., for the kind gift of pigeon egg white and Prof. Stephen D. Carrington, Dr. Mary E. Gallagher, Dr. Colm Reid and Dr. Marguerite Clyne of University College Dublin for the kind gift of mucins.peer-reviewed2020-11-1