Production Fluid

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Hui Zhang - One of the best experts on this subject based on the ideXlab platform.

  • Thermococcoides shengliensis gen. nov., sp. nov., a new member of the order Thermotogales isolated from oil-Production Fluid.
    International Journal of Systematic and Evolutionary Microbiology, 2010
    Co-Authors: Yixiao Feng, Lei Cheng, Xiaoxia Zhang, Yu Deng, Hui Zhang
    Abstract:

    A novel thermophilic, strictly anaerobic, heterotrophic bacterium, strain 2SM-2T, was isolated from the Shengli oilfield, China. This organism was identified as a member of the order Thermotogales on the basis of its 16S rRNA gene sequence and the presence of an external membranous toga-like structure. Cells stained Gram-negative, were non-motile, appeared as irregular cocci 0.7–0.9 μm in diameter, and occurred in clusters of two to six cells, with cells located within a ballooning toga-like membrane. Its optimum temperature, pH and NaCl concentration for growth were 65 °C, 7.0 and 15 g l−1, respectively. Under the optimum growth conditions, the doubling time was approximately 105 min. Strain 2SM-2T fermented a variety of simple and complex substrates such as glucose, acetate, methanol, starch and peptone while reducing elemental sulfur, sulfate and thiosulfate. The end products identified during growth on glucose were acetate, lactate, l-alanine, H2 and CO2. The DNA G+C content of this organism was 36.4 mol%. The results of 16S rRNA gene-based sequence comparisons revealed that the strain represented a new lineage within the family Thermotogaceae of the order Thermotogales. Based on the phenotypic and phylogenetic characteristics, it is proposed that this organism represents a novel species in a new genus within the family Thermotogaceae, for which the name Thermococcoides shengliensis gen. nov., sp. nov. is proposed. The type strain is 2SM-2T (=ACCC 00496T=DSM 22460T).

  • Thermococcoides shengliensis gen. nov., sp. nov., a new member of the order Thermotogales isolated from oil-Production Fluid.
    International journal of systematic and evolutionary microbiology, 2009
    Co-Authors: Yixiao Feng, Lei Cheng, Xiaoxia Zhang, Yu Deng, Hui Zhang
    Abstract:

    A novel thermophilic, strictly anaerobic, heterotrophic bacterium, strain 2SM-2(T), was isolated from the Shengli oilfield, China. This organism was identified as a member of the order Thermotogales on the basis of its 16S rRNA gene sequence and the presence of an external membranous toga-like structure. Cells stained Gram-negative, were non-motile, appeared as irregular cocci 0.7-0.9 microm in diameter, and occurred in clusters of two to six cells, with cells located within a ballooning toga-like membrane. Its optimum temperature, pH and NaCl concentration for growth were 65 degrees C, 7.0 and 15 g l(-1), respectively. Under the optimum growth conditions, the doubling time was approximately 105 min. Strain 2SM-2(T) fermented a variety of simple and complex substrates such as glucose, acetate, methanol, starch and peptone while reducing elemental sulfur, sulfate and thiosulfate. The end products identified during growth on glucose were acetate, lactate, L-alanine, H2 and CO2. The DNA G+C content of this organism was 36.4 mol%. The results of 16S rRNA gene-based sequence comparisons revealed that the strain represented a new lineage within the family Thermotogaceae of the order Thermotogales. Based on the phenotypic and phylogenetic characteristics, it is proposed that this organism represents a novel species in a new genus within the family Thermotogaceae, for which the name Thermococcoides shengliensis gen. nov., sp. nov. is proposed. The type strain is 2SM-2(T) (=ACCC 00496(T)=DSM 22460(T)).

V A Schapov - One of the best experts on this subject based on the ideXlab platform.

  • low geothermal heat flow of the urals fold belt implication of low heat Production Fluid circulation or palaeoclimate
    Tectonophysics, 1997
    Co-Authors: I T Kukkonen, I V Golovanova, Yu V Khachay, V S Druzhinin, A M Kosarev, V A Schapov
    Abstract:

    Abstract The Urals are characterized by extremely low heat flow density (HFD). We present a discussion on the relevant factors which may contribute to the observed distribution of heat flow values. The available heat flow data in the Urals and surrounding East European and West Siberian platforms are based on borehole measurements at about 300 sites which range from the Arctic Sea coast to the Kazakshtan Plain. Along the Urals fold belt heat flow density is 30 mW m−2 or less, whereas the platform areas are characterized by 20–40 mW m−2 higher values. The low heat flow density zone is 50–100 km wide. Its extension to the north is not exactly known, but the minimum extends at least to the latitude 61°N. We present new results of heat flow and heat Production measurements, Peclet number analyses on advective heat transfer by groundwater flow, as well as numerical conductive models of heat transfer in the lithosphere in the Troitsk DSS transect. The most important factor contributing to the low heat flow density in the Urals seems to be the low level of radiogenic heat Production in the crust in the Tagil-Magnitogorsk Zone. The minimum is apparently enhanced by the palaeoclimatically induced vertical variation in HFD produced by the periglacial climatic conditions during the latest glaciation epoch 70,000–10,000 years B.P. The boreholes used for HFD measurements are shallower (500–1500 m) in the Tagil-Magnitogorsk Zone than in the adjoining areas in the west (1000–3000 m) or in the east (300–3000 m), and therefore the palaeoclimatic disturbance is more pronounced in these boreholes.

  • Low geothermal heat flow of the Urals fold belt — implication of low heat Production, Fluid circulation or palaeoclimate?
    Tectonophysics, 1997
    Co-Authors: I T Kukkonen, I V Golovanova, Yu V Khachay, V S Druzhinin, A M Kosarev, V A Schapov
    Abstract:

    Abstract The Urals are characterized by extremely low heat flow density (HFD). We present a discussion on the relevant factors which may contribute to the observed distribution of heat flow values. The available heat flow data in the Urals and surrounding East European and West Siberian platforms are based on borehole measurements at about 300 sites which range from the Arctic Sea coast to the Kazakshtan Plain. Along the Urals fold belt heat flow density is 30 mW m−2 or less, whereas the platform areas are characterized by 20–40 mW m−2 higher values. The low heat flow density zone is 50–100 km wide. Its extension to the north is not exactly known, but the minimum extends at least to the latitude 61°N. We present new results of heat flow and heat Production measurements, Peclet number analyses on advective heat transfer by groundwater flow, as well as numerical conductive models of heat transfer in the lithosphere in the Troitsk DSS transect. The most important factor contributing to the low heat flow density in the Urals seems to be the low level of radiogenic heat Production in the crust in the Tagil-Magnitogorsk Zone. The minimum is apparently enhanced by the palaeoclimatically induced vertical variation in HFD produced by the periglacial climatic conditions during the latest glaciation epoch 70,000–10,000 years B.P. The boreholes used for HFD measurements are shallower (500–1500 m) in the Tagil-Magnitogorsk Zone than in the adjoining areas in the west (1000–3000 m) or in the east (300–3000 m), and therefore the palaeoclimatic disturbance is more pronounced in these boreholes.

Yixiao Feng - One of the best experts on this subject based on the ideXlab platform.

  • Thermococcoides shengliensis gen. nov., sp. nov., a new member of the order Thermotogales isolated from oil-Production Fluid.
    International Journal of Systematic and Evolutionary Microbiology, 2010
    Co-Authors: Yixiao Feng, Lei Cheng, Xiaoxia Zhang, Yu Deng, Hui Zhang
    Abstract:

    A novel thermophilic, strictly anaerobic, heterotrophic bacterium, strain 2SM-2T, was isolated from the Shengli oilfield, China. This organism was identified as a member of the order Thermotogales on the basis of its 16S rRNA gene sequence and the presence of an external membranous toga-like structure. Cells stained Gram-negative, were non-motile, appeared as irregular cocci 0.7–0.9 μm in diameter, and occurred in clusters of two to six cells, with cells located within a ballooning toga-like membrane. Its optimum temperature, pH and NaCl concentration for growth were 65 °C, 7.0 and 15 g l−1, respectively. Under the optimum growth conditions, the doubling time was approximately 105 min. Strain 2SM-2T fermented a variety of simple and complex substrates such as glucose, acetate, methanol, starch and peptone while reducing elemental sulfur, sulfate and thiosulfate. The end products identified during growth on glucose were acetate, lactate, l-alanine, H2 and CO2. The DNA G+C content of this organism was 36.4 mol%. The results of 16S rRNA gene-based sequence comparisons revealed that the strain represented a new lineage within the family Thermotogaceae of the order Thermotogales. Based on the phenotypic and phylogenetic characteristics, it is proposed that this organism represents a novel species in a new genus within the family Thermotogaceae, for which the name Thermococcoides shengliensis gen. nov., sp. nov. is proposed. The type strain is 2SM-2T (=ACCC 00496T=DSM 22460T).

  • Thermococcoides shengliensis gen. nov., sp. nov., a new member of the order Thermotogales isolated from oil-Production Fluid.
    International journal of systematic and evolutionary microbiology, 2009
    Co-Authors: Yixiao Feng, Lei Cheng, Xiaoxia Zhang, Yu Deng, Hui Zhang
    Abstract:

    A novel thermophilic, strictly anaerobic, heterotrophic bacterium, strain 2SM-2(T), was isolated from the Shengli oilfield, China. This organism was identified as a member of the order Thermotogales on the basis of its 16S rRNA gene sequence and the presence of an external membranous toga-like structure. Cells stained Gram-negative, were non-motile, appeared as irregular cocci 0.7-0.9 microm in diameter, and occurred in clusters of two to six cells, with cells located within a ballooning toga-like membrane. Its optimum temperature, pH and NaCl concentration for growth were 65 degrees C, 7.0 and 15 g l(-1), respectively. Under the optimum growth conditions, the doubling time was approximately 105 min. Strain 2SM-2(T) fermented a variety of simple and complex substrates such as glucose, acetate, methanol, starch and peptone while reducing elemental sulfur, sulfate and thiosulfate. The end products identified during growth on glucose were acetate, lactate, L-alanine, H2 and CO2. The DNA G+C content of this organism was 36.4 mol%. The results of 16S rRNA gene-based sequence comparisons revealed that the strain represented a new lineage within the family Thermotogaceae of the order Thermotogales. Based on the phenotypic and phylogenetic characteristics, it is proposed that this organism represents a novel species in a new genus within the family Thermotogaceae, for which the name Thermococcoides shengliensis gen. nov., sp. nov. is proposed. The type strain is 2SM-2(T) (=ACCC 00496(T)=DSM 22460(T)).

Yu Deng - One of the best experts on this subject based on the ideXlab platform.

  • Thermococcoides shengliensis gen. nov., sp. nov., a new member of the order Thermotogales isolated from oil-Production Fluid.
    International Journal of Systematic and Evolutionary Microbiology, 2010
    Co-Authors: Yixiao Feng, Lei Cheng, Xiaoxia Zhang, Yu Deng, Hui Zhang
    Abstract:

    A novel thermophilic, strictly anaerobic, heterotrophic bacterium, strain 2SM-2T, was isolated from the Shengli oilfield, China. This organism was identified as a member of the order Thermotogales on the basis of its 16S rRNA gene sequence and the presence of an external membranous toga-like structure. Cells stained Gram-negative, were non-motile, appeared as irregular cocci 0.7–0.9 μm in diameter, and occurred in clusters of two to six cells, with cells located within a ballooning toga-like membrane. Its optimum temperature, pH and NaCl concentration for growth were 65 °C, 7.0 and 15 g l−1, respectively. Under the optimum growth conditions, the doubling time was approximately 105 min. Strain 2SM-2T fermented a variety of simple and complex substrates such as glucose, acetate, methanol, starch and peptone while reducing elemental sulfur, sulfate and thiosulfate. The end products identified during growth on glucose were acetate, lactate, l-alanine, H2 and CO2. The DNA G+C content of this organism was 36.4 mol%. The results of 16S rRNA gene-based sequence comparisons revealed that the strain represented a new lineage within the family Thermotogaceae of the order Thermotogales. Based on the phenotypic and phylogenetic characteristics, it is proposed that this organism represents a novel species in a new genus within the family Thermotogaceae, for which the name Thermococcoides shengliensis gen. nov., sp. nov. is proposed. The type strain is 2SM-2T (=ACCC 00496T=DSM 22460T).

  • Thermococcoides shengliensis gen. nov., sp. nov., a new member of the order Thermotogales isolated from oil-Production Fluid.
    International journal of systematic and evolutionary microbiology, 2009
    Co-Authors: Yixiao Feng, Lei Cheng, Xiaoxia Zhang, Yu Deng, Hui Zhang
    Abstract:

    A novel thermophilic, strictly anaerobic, heterotrophic bacterium, strain 2SM-2(T), was isolated from the Shengli oilfield, China. This organism was identified as a member of the order Thermotogales on the basis of its 16S rRNA gene sequence and the presence of an external membranous toga-like structure. Cells stained Gram-negative, were non-motile, appeared as irregular cocci 0.7-0.9 microm in diameter, and occurred in clusters of two to six cells, with cells located within a ballooning toga-like membrane. Its optimum temperature, pH and NaCl concentration for growth were 65 degrees C, 7.0 and 15 g l(-1), respectively. Under the optimum growth conditions, the doubling time was approximately 105 min. Strain 2SM-2(T) fermented a variety of simple and complex substrates such as glucose, acetate, methanol, starch and peptone while reducing elemental sulfur, sulfate and thiosulfate. The end products identified during growth on glucose were acetate, lactate, L-alanine, H2 and CO2. The DNA G+C content of this organism was 36.4 mol%. The results of 16S rRNA gene-based sequence comparisons revealed that the strain represented a new lineage within the family Thermotogaceae of the order Thermotogales. Based on the phenotypic and phylogenetic characteristics, it is proposed that this organism represents a novel species in a new genus within the family Thermotogaceae, for which the name Thermococcoides shengliensis gen. nov., sp. nov. is proposed. The type strain is 2SM-2(T) (=ACCC 00496(T)=DSM 22460(T)).

Lei Cheng - One of the best experts on this subject based on the ideXlab platform.

  • Thermococcoides shengliensis gen. nov., sp. nov., a new member of the order Thermotogales isolated from oil-Production Fluid.
    International Journal of Systematic and Evolutionary Microbiology, 2010
    Co-Authors: Yixiao Feng, Lei Cheng, Xiaoxia Zhang, Yu Deng, Hui Zhang
    Abstract:

    A novel thermophilic, strictly anaerobic, heterotrophic bacterium, strain 2SM-2T, was isolated from the Shengli oilfield, China. This organism was identified as a member of the order Thermotogales on the basis of its 16S rRNA gene sequence and the presence of an external membranous toga-like structure. Cells stained Gram-negative, were non-motile, appeared as irregular cocci 0.7–0.9 μm in diameter, and occurred in clusters of two to six cells, with cells located within a ballooning toga-like membrane. Its optimum temperature, pH and NaCl concentration for growth were 65 °C, 7.0 and 15 g l−1, respectively. Under the optimum growth conditions, the doubling time was approximately 105 min. Strain 2SM-2T fermented a variety of simple and complex substrates such as glucose, acetate, methanol, starch and peptone while reducing elemental sulfur, sulfate and thiosulfate. The end products identified during growth on glucose were acetate, lactate, l-alanine, H2 and CO2. The DNA G+C content of this organism was 36.4 mol%. The results of 16S rRNA gene-based sequence comparisons revealed that the strain represented a new lineage within the family Thermotogaceae of the order Thermotogales. Based on the phenotypic and phylogenetic characteristics, it is proposed that this organism represents a novel species in a new genus within the family Thermotogaceae, for which the name Thermococcoides shengliensis gen. nov., sp. nov. is proposed. The type strain is 2SM-2T (=ACCC 00496T=DSM 22460T).

  • Thermococcoides shengliensis gen. nov., sp. nov., a new member of the order Thermotogales isolated from oil-Production Fluid.
    International journal of systematic and evolutionary microbiology, 2009
    Co-Authors: Yixiao Feng, Lei Cheng, Xiaoxia Zhang, Yu Deng, Hui Zhang
    Abstract:

    A novel thermophilic, strictly anaerobic, heterotrophic bacterium, strain 2SM-2(T), was isolated from the Shengli oilfield, China. This organism was identified as a member of the order Thermotogales on the basis of its 16S rRNA gene sequence and the presence of an external membranous toga-like structure. Cells stained Gram-negative, were non-motile, appeared as irregular cocci 0.7-0.9 microm in diameter, and occurred in clusters of two to six cells, with cells located within a ballooning toga-like membrane. Its optimum temperature, pH and NaCl concentration for growth were 65 degrees C, 7.0 and 15 g l(-1), respectively. Under the optimum growth conditions, the doubling time was approximately 105 min. Strain 2SM-2(T) fermented a variety of simple and complex substrates such as glucose, acetate, methanol, starch and peptone while reducing elemental sulfur, sulfate and thiosulfate. The end products identified during growth on glucose were acetate, lactate, L-alanine, H2 and CO2. The DNA G+C content of this organism was 36.4 mol%. The results of 16S rRNA gene-based sequence comparisons revealed that the strain represented a new lineage within the family Thermotogaceae of the order Thermotogales. Based on the phenotypic and phylogenetic characteristics, it is proposed that this organism represents a novel species in a new genus within the family Thermotogaceae, for which the name Thermococcoides shengliensis gen. nov., sp. nov. is proposed. The type strain is 2SM-2(T) (=ACCC 00496(T)=DSM 22460(T)).