The Experts below are selected from a list of 309 Experts worldwide ranked by ideXlab platform
Hesham Ali Elenshasy - One of the best experts on this subject based on the ideXlab platform.
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Production of β galactosidase in shake flask and stirred tank bioreactor cultivations by a newly isolated bacillus licheniformis strain
Biocatalysis and agricultural biotechnology, 2019Co-Authors: Elsayed A Elsayed, Enas N Danial, Mohammed A M Wadaan, Hesham Ali ElenshasyAbstract:Abstract β-Galactosidase is an industrially important enzyme with many applications in various health, diagnostic and food processing industries. The present work describes the isolation and identification of a new isolate capable of producing β-galactosidase. The work was further extended to optimize the Production Medium and investigate the kinetics of cell growth and enzyme Production during batch cultivation in shake-flask and 5L-stirred tank bioreactor. The isolated strain was identified as Bacillus licheniformis using 16S rRNA and BLAST analysis. The final optimized Production Medium contained (g/L): yeast extract, 14.0; lactose, 8.0; NaCl, 16.0; (NH4)H2PO4, 0.1; MgSO4.7H2O, 0.1; K2HPO4, 0.1. The optimized Medium afforded maximal β-galactosidase Production of 116.4 U/mL with a specific yield coefficient (YP/X) of 27.6 × 103 U/g cells. These results corresponds to an increase of about 4.4- and 2.8-folds, respectively, than the initial un-optimized Production Medium. Further improvement in the Production process was achieved by cultivating cells in 5L-stirred tank bioreactor, which increased volumetric enzyme productivity and specific yield coefficient by 8.5- and 6.0-folds than the initial un-optimized Production Medium, respectively. A new β-galactosidase producing strain was identified and the volumetric as well as specific productivities were greatly enhanced through Medium optimization and bioreactor cultivation.
Nordin H. Lajis - One of the best experts on this subject based on the ideXlab platform.
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Growth and anthraquinone Production of Morinda elliptica cell suspension cultures in a stirred-tank bioreactor.
Journal of agricultural and food chemistry, 2000Co-Authors: Mohd Azmuddin Abdullah, Arbakariya B. Ariff, Mahmood Marziah, And Abdul M. Ali, Nordin H. LajisAbstract:The effects of Medium strategy, number of impellers, aeration mode, and mode of operation on Morinda elliptica cell suspension cultures in a stirred-tank bioreactor are described. A lower number of impellers and continuous aeration contributed toward high cell growth rate, whereas a higher number of impellers reduced cell growth rate, although not anthraquinone yield. The semicontinuous mode could indirectly imitate the larger scale version of Production Medium strategy and improved anthraquinone Production even with 0.012% (v/v) antifoam addition. Production Medium promoted both growth (maximum dry cell weight of 24.6 g/L) and anthraquinone formation (maximum content of 19.5 mg/g of dry cell weight), without any necessity for antifoam addition. Cultures in Production Medium or with higher growth rate and anthraquinone Production were less acidic than cultures in growth Medium or with lower growth rate and anthraquinone Production. Using the best operating variables, growth of M. elliptica cells (24.6 g/L) and anthraquinone yield (0.25 g/L) were 45% and 140%, respectively, lower than those using a shake flask culture after 12 days of cultivation.
Mohd Azmuddin Abdullah - One of the best experts on this subject based on the ideXlab platform.
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Enhanced anthraquinones Production from adsorbent-treated Morinda elliptica cell suspension cultures in Production Medium strategy
Process Biochemistry, 2007Co-Authors: Lim Chiang, Mohd Azmuddin AbdullahAbstract:Abstract Continuous removal of anthraquinones (AQ) by Amberlite polymeric adsorbents (XAD-4, XAD-7 and XAD-16) through in situ adsorption in Morinda elliptica cell suspension cultures is studied for product recovery and improvement of the overall titre. Ethanol was the best eluting solvent for effective recovery of AQ from all adsorbents. Pre-treatment of XAD-4 with sodium acetate not only enhanced intracellular AQ, but also AQ release and subsequent recovery from the adsorbent. The addition of sodium acetate pretreated XAD-4 on day 18 for 6-day contact period, achieved comparable cell growth to control (41 g/L), but with 1.3-fold higher intracellular AQ (124 mg/g DW) and two-fold increase in extracellular AQ (14.3 mg/L). High amount of adsorbent and longer contact period for the cultures entering stationary growth phase, stimulated AQ release and recovery but at the expense of cell growth. With 5–8.3 g XAD-4 adsorbent per litre M. elliptica culture in Production (P) Medium, between 60 and 90% AQ was recovered from extracellular AQ after 24–26 days of culture period.
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Growth and anthraquinone Production of Morinda elliptica cell suspension cultures in a stirred-tank bioreactor.
Journal of agricultural and food chemistry, 2000Co-Authors: Mohd Azmuddin Abdullah, Arbakariya B. Ariff, Mahmood Marziah, And Abdul M. Ali, Nordin H. LajisAbstract:The effects of Medium strategy, number of impellers, aeration mode, and mode of operation on Morinda elliptica cell suspension cultures in a stirred-tank bioreactor are described. A lower number of impellers and continuous aeration contributed toward high cell growth rate, whereas a higher number of impellers reduced cell growth rate, although not anthraquinone yield. The semicontinuous mode could indirectly imitate the larger scale version of Production Medium strategy and improved anthraquinone Production even with 0.012% (v/v) antifoam addition. Production Medium promoted both growth (maximum dry cell weight of 24.6 g/L) and anthraquinone formation (maximum content of 19.5 mg/g of dry cell weight), without any necessity for antifoam addition. Cultures in Production Medium or with higher growth rate and anthraquinone Production were less acidic than cultures in growth Medium or with lower growth rate and anthraquinone Production. Using the best operating variables, growth of M. elliptica cells (24.6 g/L) and anthraquinone yield (0.25 g/L) were 45% and 140%, respectively, lower than those using a shake flask culture after 12 days of cultivation.
Elsayed A Elsayed - One of the best experts on this subject based on the ideXlab platform.
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Production of β galactosidase in shake flask and stirred tank bioreactor cultivations by a newly isolated bacillus licheniformis strain
Biocatalysis and agricultural biotechnology, 2019Co-Authors: Elsayed A Elsayed, Enas N Danial, Mohammed A M Wadaan, Hesham Ali ElenshasyAbstract:Abstract β-Galactosidase is an industrially important enzyme with many applications in various health, diagnostic and food processing industries. The present work describes the isolation and identification of a new isolate capable of producing β-galactosidase. The work was further extended to optimize the Production Medium and investigate the kinetics of cell growth and enzyme Production during batch cultivation in shake-flask and 5L-stirred tank bioreactor. The isolated strain was identified as Bacillus licheniformis using 16S rRNA and BLAST analysis. The final optimized Production Medium contained (g/L): yeast extract, 14.0; lactose, 8.0; NaCl, 16.0; (NH4)H2PO4, 0.1; MgSO4.7H2O, 0.1; K2HPO4, 0.1. The optimized Medium afforded maximal β-galactosidase Production of 116.4 U/mL with a specific yield coefficient (YP/X) of 27.6 × 103 U/g cells. These results corresponds to an increase of about 4.4- and 2.8-folds, respectively, than the initial un-optimized Production Medium. Further improvement in the Production process was achieved by cultivating cells in 5L-stirred tank bioreactor, which increased volumetric enzyme productivity and specific yield coefficient by 8.5- and 6.0-folds than the initial un-optimized Production Medium, respectively. A new β-galactosidase producing strain was identified and the volumetric as well as specific productivities were greatly enhanced through Medium optimization and bioreactor cultivation.
Mohammed A M Wadaan - One of the best experts on this subject based on the ideXlab platform.
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Production of β galactosidase in shake flask and stirred tank bioreactor cultivations by a newly isolated bacillus licheniformis strain
Biocatalysis and agricultural biotechnology, 2019Co-Authors: Elsayed A Elsayed, Enas N Danial, Mohammed A M Wadaan, Hesham Ali ElenshasyAbstract:Abstract β-Galactosidase is an industrially important enzyme with many applications in various health, diagnostic and food processing industries. The present work describes the isolation and identification of a new isolate capable of producing β-galactosidase. The work was further extended to optimize the Production Medium and investigate the kinetics of cell growth and enzyme Production during batch cultivation in shake-flask and 5L-stirred tank bioreactor. The isolated strain was identified as Bacillus licheniformis using 16S rRNA and BLAST analysis. The final optimized Production Medium contained (g/L): yeast extract, 14.0; lactose, 8.0; NaCl, 16.0; (NH4)H2PO4, 0.1; MgSO4.7H2O, 0.1; K2HPO4, 0.1. The optimized Medium afforded maximal β-galactosidase Production of 116.4 U/mL with a specific yield coefficient (YP/X) of 27.6 × 103 U/g cells. These results corresponds to an increase of about 4.4- and 2.8-folds, respectively, than the initial un-optimized Production Medium. Further improvement in the Production process was achieved by cultivating cells in 5L-stirred tank bioreactor, which increased volumetric enzyme productivity and specific yield coefficient by 8.5- and 6.0-folds than the initial un-optimized Production Medium, respectively. A new β-galactosidase producing strain was identified and the volumetric as well as specific productivities were greatly enhanced through Medium optimization and bioreactor cultivation.
