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William F. Blakemore - One of the best experts on this subject based on the ideXlab platform.
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Transplanted CG4 Cells (an Oligodendrocyte Progenitor Cell Line) Survive, Migrate, and Contribute to Repair of Areas of Demyelination in X-Irradiated and Damaged Spinal Cord but Not in Normal Spinal Cord
Experimental neurology, 1996Co-Authors: Robin J M Franklin, Susan A. Bayley, William F. BlakemoreAbstract:Abstract In this study, we have examined the behavior of alac-Z-transfected O-2A Progenitor Cell Line, CG4, following transplantation into normal and X-irradiated adult rat spinal cord, and we have also addressed the issue of whether CG4 Cells transplanted remotely from ethidium bromide-induced demyelinating lesions in both X-irradiated and nonirradiated spinal cord are able to contribute to their repair. Following transplantation into X-irradiated spinal cord, CG4 Cells survive, divide, and migrate extensively. The migration occurs mainly within the parenchymal tissue of the cord without preference for white or gray matter. Moreover, CG4 Cells migrating away from their point of introduction are able to enter areas of demyelination and remyelinate the demyelinated axons therein. In contrast, when CG4 Cells are transplanted into nonirradiated spinal cord, their survival is limited to areas of damage created by the injection procedure. The CG4 Cells do not survive in undamaged, nonirradiated spinal cord. When transplanted remotely from areas of demyelination they are unable to traverse intervening areas of normal white matter, although they may enter lesions if transplanted into their close vicinity. These results have important implications for the development of potential therapeutic strategies for the treatment of multifocal demyelinating disorders that are based on glial Cell transplantation.
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In vitro and In vivo Analysis of a Rat Bipotential O – 2A Progenitor Cell Line Containing the Temperature‐sensitive Mutant Gene of the SV40 Large T Antigen
The European journal of neuroscience, 1993Co-Authors: Susan C Barnett, Robin J M Franklin, William F. BlakemoreAbstract:Primary cultures from neonatal rat optic nerve contain pluripotential O – 2A Progenitor Cells that are capable of differentiating into oligodendrocytes, type-2 astrocytes or adult O – 2A Progenitors (O – 2Aadult). Since primary optic nerve cultures contain a mixture of glial Cell types of which only a small number are O – 2A Progenitors, experiments on Cell Lineage and differentiation carried out using these cultures are both intrinsically limited and difficult to interpret. Ideally, Cells from a clonal Cell population would provide the optimal starting material for biological studies. In this paper we describe the creation of an O – 2A Progenitor Cell Line using a retrovirus carrying a temperature-sensitive mutant SV40 large T antigen gene. This Cell Line has provided sufficient numbers of Cells to allow analysis of their in vitro properties and their behaviour following transplantation into an in vivo environment. At the non-permissive temperature (39°C), these Cells differentiate into oligodendrocytes and type-2 astrocytes in a similar fashion to O – 2A Progenitor Cells from primary cultures (O – 2Aprim). When grown in media containing platelet-derived growth factor and basic fibroblast growth factor, the Cell numbers can be expanded in culture without differentiating, consistent with the behaviour of O – 2Aprim Progenitor Cells. By exploiting this property, it has been possible to culture large numbers of O – 2A Progenitors for in vivo analysis. In this study we have shown that transplantation of this O – 2A Cell Line into glia-free areas in adult rat spinal cord results in differentiation of a proportion of Cells into oligodendrocytes which are capable of myelinating axons. Furthermore, differentiation of O – 2A Cells into astrocytes was also observed, indicating that the bipotentiality of these Cells in vitro can also be demonstrated in vivo.
Robin J M Franklin - One of the best experts on this subject based on the ideXlab platform.
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Transplanted CG4 Cells (an Oligodendrocyte Progenitor Cell Line) Survive, Migrate, and Contribute to Repair of Areas of Demyelination in X-Irradiated and Damaged Spinal Cord but Not in Normal Spinal Cord
Experimental neurology, 1996Co-Authors: Robin J M Franklin, Susan A. Bayley, William F. BlakemoreAbstract:Abstract In this study, we have examined the behavior of alac-Z-transfected O-2A Progenitor Cell Line, CG4, following transplantation into normal and X-irradiated adult rat spinal cord, and we have also addressed the issue of whether CG4 Cells transplanted remotely from ethidium bromide-induced demyelinating lesions in both X-irradiated and nonirradiated spinal cord are able to contribute to their repair. Following transplantation into X-irradiated spinal cord, CG4 Cells survive, divide, and migrate extensively. The migration occurs mainly within the parenchymal tissue of the cord without preference for white or gray matter. Moreover, CG4 Cells migrating away from their point of introduction are able to enter areas of demyelination and remyelinate the demyelinated axons therein. In contrast, when CG4 Cells are transplanted into nonirradiated spinal cord, their survival is limited to areas of damage created by the injection procedure. The CG4 Cells do not survive in undamaged, nonirradiated spinal cord. When transplanted remotely from areas of demyelination they are unable to traverse intervening areas of normal white matter, although they may enter lesions if transplanted into their close vicinity. These results have important implications for the development of potential therapeutic strategies for the treatment of multifocal demyelinating disorders that are based on glial Cell transplantation.
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in vitro and in vivo analysis of a rat bipotential o 2a Progenitor Cell Line containing the temperature sensitive mutant gene of the sv40 large t antigen
European Journal of Neuroscience, 1993Co-Authors: Susan C Barnett, Robin J M Franklin, W F BlakemoreAbstract:Primary cultures from neonatal rat optic nerve contain pluripotential O – 2A Progenitor Cells that are capable of differentiating into oligodendrocytes, type-2 astrocytes or adult O – 2A Progenitors (O – 2Aadult). Since primary optic nerve cultures contain a mixture of glial Cell types of which only a small number are O – 2A Progenitors, experiments on Cell Lineage and differentiation carried out using these cultures are both intrinsically limited and difficult to interpret. Ideally, Cells from a clonal Cell population would provide the optimal starting material for biological studies. In this paper we describe the creation of an O – 2A Progenitor Cell Line using a retrovirus carrying a temperature-sensitive mutant SV40 large T antigen gene. This Cell Line has provided sufficient numbers of Cells to allow analysis of their in vitro properties and their behaviour following transplantation into an in vivo environment. At the non-permissive temperature (39°C), these Cells differentiate into oligodendrocytes and type-2 astrocytes in a similar fashion to O – 2A Progenitor Cells from primary cultures (O – 2Aprim). When grown in media containing platelet-derived growth factor and basic fibroblast growth factor, the Cell numbers can be expanded in culture without differentiating, consistent with the behaviour of O – 2Aprim Progenitor Cells. By exploiting this property, it has been possible to culture large numbers of O – 2A Progenitors for in vivo analysis. In this study we have shown that transplantation of this O – 2A Cell Line into glia-free areas in adult rat spinal cord results in differentiation of a proportion of Cells into oligodendrocytes which are capable of myelinating axons. Furthermore, differentiation of O – 2A Cells into astrocytes was also observed, indicating that the bipotentiality of these Cells in vitro can also be demonstrated in vivo.
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In vitro and In vivo Analysis of a Rat Bipotential O – 2A Progenitor Cell Line Containing the Temperature‐sensitive Mutant Gene of the SV40 Large T Antigen
The European journal of neuroscience, 1993Co-Authors: Susan C Barnett, Robin J M Franklin, William F. BlakemoreAbstract:Primary cultures from neonatal rat optic nerve contain pluripotential O – 2A Progenitor Cells that are capable of differentiating into oligodendrocytes, type-2 astrocytes or adult O – 2A Progenitors (O – 2Aadult). Since primary optic nerve cultures contain a mixture of glial Cell types of which only a small number are O – 2A Progenitors, experiments on Cell Lineage and differentiation carried out using these cultures are both intrinsically limited and difficult to interpret. Ideally, Cells from a clonal Cell population would provide the optimal starting material for biological studies. In this paper we describe the creation of an O – 2A Progenitor Cell Line using a retrovirus carrying a temperature-sensitive mutant SV40 large T antigen gene. This Cell Line has provided sufficient numbers of Cells to allow analysis of their in vitro properties and their behaviour following transplantation into an in vivo environment. At the non-permissive temperature (39°C), these Cells differentiate into oligodendrocytes and type-2 astrocytes in a similar fashion to O – 2A Progenitor Cells from primary cultures (O – 2Aprim). When grown in media containing platelet-derived growth factor and basic fibroblast growth factor, the Cell numbers can be expanded in culture without differentiating, consistent with the behaviour of O – 2Aprim Progenitor Cells. By exploiting this property, it has been possible to culture large numbers of O – 2A Progenitors for in vivo analysis. In this study we have shown that transplantation of this O – 2A Cell Line into glia-free areas in adult rat spinal cord results in differentiation of a proportion of Cells into oligodendrocytes which are capable of myelinating axons. Furthermore, differentiation of O – 2A Cells into astrocytes was also observed, indicating that the bipotentiality of these Cells in vitro can also be demonstrated in vivo.
Susan C Barnett - One of the best experts on this subject based on the ideXlab platform.
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in vitro and in vivo analysis of a rat bipotential o 2a Progenitor Cell Line containing the temperature sensitive mutant gene of the sv40 large t antigen
European Journal of Neuroscience, 1993Co-Authors: Susan C Barnett, Robin J M Franklin, W F BlakemoreAbstract:Primary cultures from neonatal rat optic nerve contain pluripotential O – 2A Progenitor Cells that are capable of differentiating into oligodendrocytes, type-2 astrocytes or adult O – 2A Progenitors (O – 2Aadult). Since primary optic nerve cultures contain a mixture of glial Cell types of which only a small number are O – 2A Progenitors, experiments on Cell Lineage and differentiation carried out using these cultures are both intrinsically limited and difficult to interpret. Ideally, Cells from a clonal Cell population would provide the optimal starting material for biological studies. In this paper we describe the creation of an O – 2A Progenitor Cell Line using a retrovirus carrying a temperature-sensitive mutant SV40 large T antigen gene. This Cell Line has provided sufficient numbers of Cells to allow analysis of their in vitro properties and their behaviour following transplantation into an in vivo environment. At the non-permissive temperature (39°C), these Cells differentiate into oligodendrocytes and type-2 astrocytes in a similar fashion to O – 2A Progenitor Cells from primary cultures (O – 2Aprim). When grown in media containing platelet-derived growth factor and basic fibroblast growth factor, the Cell numbers can be expanded in culture without differentiating, consistent with the behaviour of O – 2Aprim Progenitor Cells. By exploiting this property, it has been possible to culture large numbers of O – 2A Progenitors for in vivo analysis. In this study we have shown that transplantation of this O – 2A Cell Line into glia-free areas in adult rat spinal cord results in differentiation of a proportion of Cells into oligodendrocytes which are capable of myelinating axons. Furthermore, differentiation of O – 2A Cells into astrocytes was also observed, indicating that the bipotentiality of these Cells in vitro can also be demonstrated in vivo.
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In vitro and In vivo Analysis of a Rat Bipotential O – 2A Progenitor Cell Line Containing the Temperature‐sensitive Mutant Gene of the SV40 Large T Antigen
The European journal of neuroscience, 1993Co-Authors: Susan C Barnett, Robin J M Franklin, William F. BlakemoreAbstract:Primary cultures from neonatal rat optic nerve contain pluripotential O – 2A Progenitor Cells that are capable of differentiating into oligodendrocytes, type-2 astrocytes or adult O – 2A Progenitors (O – 2Aadult). Since primary optic nerve cultures contain a mixture of glial Cell types of which only a small number are O – 2A Progenitors, experiments on Cell Lineage and differentiation carried out using these cultures are both intrinsically limited and difficult to interpret. Ideally, Cells from a clonal Cell population would provide the optimal starting material for biological studies. In this paper we describe the creation of an O – 2A Progenitor Cell Line using a retrovirus carrying a temperature-sensitive mutant SV40 large T antigen gene. This Cell Line has provided sufficient numbers of Cells to allow analysis of their in vitro properties and their behaviour following transplantation into an in vivo environment. At the non-permissive temperature (39°C), these Cells differentiate into oligodendrocytes and type-2 astrocytes in a similar fashion to O – 2A Progenitor Cells from primary cultures (O – 2Aprim). When grown in media containing platelet-derived growth factor and basic fibroblast growth factor, the Cell numbers can be expanded in culture without differentiating, consistent with the behaviour of O – 2Aprim Progenitor Cells. By exploiting this property, it has been possible to culture large numbers of O – 2A Progenitors for in vivo analysis. In this study we have shown that transplantation of this O – 2A Cell Line into glia-free areas in adult rat spinal cord results in differentiation of a proportion of Cells into oligodendrocytes which are capable of myelinating axons. Furthermore, differentiation of O – 2A Cells into astrocytes was also observed, indicating that the bipotentiality of these Cells in vitro can also be demonstrated in vivo.
Akifumi Akamine - One of the best experts on this subject based on the ideXlab platform.
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Effects of TGF-β1 on the proliferation and differentiation of human periodontal ligament Cells and a human periodontal ligament stem/Progenitor Cell Line
Cell and tissue research, 2010Co-Authors: Shinsuke Fujii, Hidefumi Maeda, Atsushi Tomokiyo, Satoshi Monnouchi, Kiyomi Hori, Naohisa Wada, Akifumi AkamineAbstract:Periodontal ligament (PDL) is a specialized connective tissue that influences the lifespan of the tooth. Transforming growth factor-β1 (TGF-β1) is a multifunctional cytokine, but little is known about the effects of TGF-β1 on PDL Cells. Our aim has been to demonstrate the expression of TGF-β1 in rat PDL tissues and to evaluate its effects on the proliferation and gene expression in human PDL Cells (HPLCs) and a human PDL stem/Progenitor Cell Line, Line 1-11, that we have recently developed. The expression of TGF-β1 in the entire PDL tissue was confirmed immunohistochemically, and both HPLCs and Cell Line 1-11 expressed mRNA from the TGF-β1, TGF-β type I receptor, and TGF-β type II receptor genes. Although exogenous TGF-β1 stimulated the proliferation of HPLCs, it did not upregulate the expression of alpha-smooth muscle actin (α-SMA), type I collagen (Col I), or fibrillin-1 (FBN1) mRNA or of α-SMA protein in HPLCs, whereas expression for these genes was attenuated by an anti-TGF-β1 neutralizing antibody. In contrast, exogenous TGF-β1 reduced the proliferation of Cell Line 1-11, although it upregulated the expression of α-SMA, Col I, and FBN1 mRNA and of α-SMA protein in this Cell Line. In addition, interleukin-1 beta stimulation significantly reduced the expression of TGF-β1 mRNA and protein in HPLCs. Thus, TGF-β1 seems to play an important role in inducing fibroblastic differentiation of PDL stem/Progenitor Cells and in maintaining the PDL apparatus under physiological conditions.
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effects of tgf β1 on the proliferation and differentiation of human periodontal ligament Cells and a human periodontal ligament stem Progenitor Cell Line
Cell and Tissue Research, 2010Co-Authors: Shinsuke Fujii, Hidefumi Maeda, Atsushi Tomokiyo, Satoshi Monnouchi, Kiyomi Hori, Naohisa Wada, Akifumi AkamineAbstract:Periodontal ligament (PDL) is a specialized connective tissue that influences the lifespan of the tooth. Transforming growth factor-β1 (TGF-β1) is a multifunctional cytokine, but little is known about the effects of TGF-β1 on PDL Cells. Our aim has been to demonstrate the expression of TGF-β1 in rat PDL tissues and to evaluate its effects on the proliferation and gene expression in human PDL Cells (HPLCs) and a human PDL stem/Progenitor Cell Line, Line 1-11, that we have recently developed. The expression of TGF-β1 in the entire PDL tissue was confirmed immunohistochemically, and both HPLCs and Cell Line 1-11 expressed mRNA from the TGF-β1, TGF-β type I receptor, and TGF-β type II receptor genes. Although exogenous TGF-β1 stimulated the proliferation of HPLCs, it did not upregulate the expression of alpha-smooth muscle actin (α-SMA), type I collagen (Col I), or fibrillin-1 (FBN1) mRNA or of α-SMA protein in HPLCs, whereas expression for these genes was attenuated by an anti-TGF-β1 neutralizing antibody. In contrast, exogenous TGF-β1 reduced the proliferation of Cell Line 1-11, although it upregulated the expression of α-SMA, Col I, and FBN1 mRNA and of α-SMA protein in this Cell Line. In addition, interleukin-1 beta stimulation significantly reduced the expression of TGF-β1 mRNA and protein in HPLCs. Thus, TGF-β1 seems to play an important role in inducing fibroblastic differentiation of PDL stem/Progenitor Cells and in maintaining the PDL apparatus under physiological conditions.
W F Blakemore - One of the best experts on this subject based on the ideXlab platform.
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in vitro and in vivo analysis of a rat bipotential o 2a Progenitor Cell Line containing the temperature sensitive mutant gene of the sv40 large t antigen
European Journal of Neuroscience, 1993Co-Authors: Susan C Barnett, Robin J M Franklin, W F BlakemoreAbstract:Primary cultures from neonatal rat optic nerve contain pluripotential O – 2A Progenitor Cells that are capable of differentiating into oligodendrocytes, type-2 astrocytes or adult O – 2A Progenitors (O – 2Aadult). Since primary optic nerve cultures contain a mixture of glial Cell types of which only a small number are O – 2A Progenitors, experiments on Cell Lineage and differentiation carried out using these cultures are both intrinsically limited and difficult to interpret. Ideally, Cells from a clonal Cell population would provide the optimal starting material for biological studies. In this paper we describe the creation of an O – 2A Progenitor Cell Line using a retrovirus carrying a temperature-sensitive mutant SV40 large T antigen gene. This Cell Line has provided sufficient numbers of Cells to allow analysis of their in vitro properties and their behaviour following transplantation into an in vivo environment. At the non-permissive temperature (39°C), these Cells differentiate into oligodendrocytes and type-2 astrocytes in a similar fashion to O – 2A Progenitor Cells from primary cultures (O – 2Aprim). When grown in media containing platelet-derived growth factor and basic fibroblast growth factor, the Cell numbers can be expanded in culture without differentiating, consistent with the behaviour of O – 2Aprim Progenitor Cells. By exploiting this property, it has been possible to culture large numbers of O – 2A Progenitors for in vivo analysis. In this study we have shown that transplantation of this O – 2A Cell Line into glia-free areas in adult rat spinal cord results in differentiation of a proportion of Cells into oligodendrocytes which are capable of myelinating axons. Furthermore, differentiation of O – 2A Cells into astrocytes was also observed, indicating that the bipotentiality of these Cells in vitro can also be demonstrated in vivo.