Progenitor Cell

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Philip H. Jones - One of the best experts on this subject based on the ideXlab platform.

  • a single type of Progenitor Cell maintains normal epidermis
    Nature, 2007
    Co-Authors: Elizabeth Clayton, Benjamin D Simons, David P Doupe, Allon M Klein, Douglas J Winton, Philip H. Jones
    Abstract:

    For more than 30 years, the accepted model of epithelial homeostasis has assumed that skin tissue is maintained by two separate populations of Progenitor Cells. Self-renewing stem Cells were thought to give rise to short-lived Progenitors that in turn form the new epidermis. Clayton et al. now suggest that this hypothesis can be replaced with a simpler model in which a single type of Progenitor Cell undergoes asymmetric division at a rate that ensures epidermal homeostasis. An alternative model for the homeostasis of adult epidermis posits posits only one type of stem Cell undergoes both symmetric and asymmetric divisions to ensure epidermal homeostasis. A genetic approach of marking single Cells in the adult mouse tail epidermis shows that the clones of labelled Cells that arise from their inducible labelling approach are most likely to come from a single compartment of proliferating Cells, which may undergo an unlimited number of divisions. According to the current model of adult epidermal homeostasis, skin tissue is maintained by two discrete populations of Progenitor Cells: self-renewing stem Cells; and their progeny, known as transit amplifying Cells, which differentiate after several rounds of Cell division1,2,3. By making use of inducible genetic labelling, we have tracked the fate of a representative sample of Progenitor Cells in mouse tail epidermis at single-Cell resolution in vivo at time intervals up to one year. Here we show that clone-size distributions are consistent with a new model of homeostasis involving only one type of Progenitor Cell. These Cells are found to undergo both symmetric and asymmetric division at rates that ensure epidermal homeostasis. The results raise important questions about the potential role of stem Cells on tissue maintenance in vivo.

  • a single type of Progenitor Cell maintains normal epidermis
    Nature, 2007
    Co-Authors: Elizabeth Clayton, Benjamin D Simons, David P Doupe, Allon M Klein, Douglas J Winton, Philip H. Jones
    Abstract:

    According to the current model of adult epidermal homeostasis, skin tissue is maintained by two discrete populations of Progenitor Cells: self-renewing stem Cells; and their progeny, known as transit amplifying Cells, which differentiate after several rounds of Cell division. By making use of inducible genetic labelling, we have tracked the fate of a representative sample of Progenitor Cells in mouse tail epidermis at single-Cell resolution in vivo at time intervals up to one year. Here we show that clone-size distributions are consistent with a new model of homeostasis involving only one type of Progenitor Cell. These Cells are found to undergo both symmetric and asymmetric division at rates that ensure epidermal homeostasis. The results raise important questions about the potential role of stem Cells on tissue maintenance in vivo.

Brian J. Bolwell - One of the best experts on this subject based on the ideXlab platform.

  • blood Progenitor Cell separation from clinical leukapheresis product by magnetic nanoparticle binding and magnetophoresis
    Biotechnology and Bioengineering, 2007
    Co-Authors: Lee R. Moore, Brian J. Bolwell, Jeffrey J. Chalmers, Ying Jing, Stephen P Williams, Sherif S Farag, Maciej Zborowski
    Abstract:

    Positive selection of CD34+ blood Progenitor Cells from circulation has been reported to improve patient recovery in applications of autologous transplantation. Current magnetic separation methods rely on Cell capture and release on solid supports rather than sorting from flowing suspensions, which limits the range of therapeutic applications and the process scale up. We tested CD34+ Cell immunomagnetic labeling and isolation from fresh leukocyte fraction of peripheral blood (leukapheresis) using the continuous quadrupole magnetic flow sorter (QMS), consisting of a flow channel (SHOT, Greenville, IN) and a quadrupole magnet with a maximum field intensity (B o ) of 1.42 T and a mean force field strength (S m ) of 1.45 × 10 8 TA/m 2 . Both the sample magnetophoretic mobility (m) and the inlet and outlet flow patterns highly affect the QMS performance. Seven commercial Progenitor Cell labeling reagent combinations were quantitatively evaluated by measuring magnetophoretic mobility of a high CD34 expression Cell line, KG-la, using the Cell tracking velocimeter (CTV). The CD34 Progenitor Cell Isolation Kit™ (Miltenyi Biotec, Bergisch Gladbach, Germany) showed the strongest labeling of KG- la Cells and was selected for Progenitor Cell enrichment from 11 fresh and 11 cryopreserved clinical leukapheresis samples derived from different donors. The CD34+ Cells were isolated with a purity of 60-96%, a recovery of 18-60%, an enrichment rate of 12-169, and a throughput of (1.7-9.3) x 10 4 Cells/s. The results also showed a highly regular dependence of the QMS performance on the flow conditions that agreed with the theoretical predictions based on the CD34+ Cell magnetophoretic mobility.

  • Progenitor Cell isolation with a high-capacity quadrupole magnetic flow sorter
    Journal of Magnetism and Magnetic Materials, 2001
    Co-Authors: Lee R. Moore, Alexander Rodriguez, P. Stephen Williams, Kara E. Mccloskey, Brian J. Bolwell, Masayuki Nakamura, Jeffrey J. Chalmers, Maciej Zborowski
    Abstract:

    Abstract Rapid and efficient sorting is important for Progenitor Cell isolation. Therefore, we have built and evaluated a high-speed, continuous flow, quadrupole magnetic sorter. The cross-over behavior of non-magnetic particles, combined with separation theory is used to optimize performance. CD34+ Progenitor Cells are separated to purities from 64–95% and a throughput of 10 7  Cells/s.

  • variables associated with the platelet count 6 weeks after autologous peripheral blood Progenitor Cell transplantation
    Bone Marrow Transplantation, 1998
    Co-Authors: Brian J. Bolwell, Marlene Goormastic, Steven Andresen, Anna Koo, K Wise, Beth Overmoyer, Brad Pohlman, Matt Kalaycio
    Abstract:

    Variables associated with the platelet count 6 weeks after autologous peripheral blood Progenitor Cell transplantation

Matt Kalaycio - One of the best experts on this subject based on the ideXlab platform.

Elizabeth Clayton - One of the best experts on this subject based on the ideXlab platform.

  • a single type of Progenitor Cell maintains normal epidermis
    Nature, 2007
    Co-Authors: Elizabeth Clayton, Benjamin D Simons, David P Doupe, Allon M Klein, Douglas J Winton, Philip H. Jones
    Abstract:

    For more than 30 years, the accepted model of epithelial homeostasis has assumed that skin tissue is maintained by two separate populations of Progenitor Cells. Self-renewing stem Cells were thought to give rise to short-lived Progenitors that in turn form the new epidermis. Clayton et al. now suggest that this hypothesis can be replaced with a simpler model in which a single type of Progenitor Cell undergoes asymmetric division at a rate that ensures epidermal homeostasis. An alternative model for the homeostasis of adult epidermis posits posits only one type of stem Cell undergoes both symmetric and asymmetric divisions to ensure epidermal homeostasis. A genetic approach of marking single Cells in the adult mouse tail epidermis shows that the clones of labelled Cells that arise from their inducible labelling approach are most likely to come from a single compartment of proliferating Cells, which may undergo an unlimited number of divisions. According to the current model of adult epidermal homeostasis, skin tissue is maintained by two discrete populations of Progenitor Cells: self-renewing stem Cells; and their progeny, known as transit amplifying Cells, which differentiate after several rounds of Cell division1,2,3. By making use of inducible genetic labelling, we have tracked the fate of a representative sample of Progenitor Cells in mouse tail epidermis at single-Cell resolution in vivo at time intervals up to one year. Here we show that clone-size distributions are consistent with a new model of homeostasis involving only one type of Progenitor Cell. These Cells are found to undergo both symmetric and asymmetric division at rates that ensure epidermal homeostasis. The results raise important questions about the potential role of stem Cells on tissue maintenance in vivo.

  • a single type of Progenitor Cell maintains normal epidermis
    Nature, 2007
    Co-Authors: Elizabeth Clayton, Benjamin D Simons, David P Doupe, Allon M Klein, Douglas J Winton, Philip H. Jones
    Abstract:

    According to the current model of adult epidermal homeostasis, skin tissue is maintained by two discrete populations of Progenitor Cells: self-renewing stem Cells; and their progeny, known as transit amplifying Cells, which differentiate after several rounds of Cell division. By making use of inducible genetic labelling, we have tracked the fate of a representative sample of Progenitor Cells in mouse tail epidermis at single-Cell resolution in vivo at time intervals up to one year. Here we show that clone-size distributions are consistent with a new model of homeostasis involving only one type of Progenitor Cell. These Cells are found to undergo both symmetric and asymmetric division at rates that ensure epidermal homeostasis. The results raise important questions about the potential role of stem Cells on tissue maintenance in vivo.

Volker Diehl - One of the best experts on this subject based on the ideXlab platform.

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