The Experts below are selected from a list of 153348 Experts worldwide ranked by ideXlab platform
Seeram Ramakrishna - One of the best experts on this subject based on the ideXlab platform.
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naturally derived biofunctional nanofibrous scaffold for Skin Tissue regeneration
International Journal of Biological Macromolecules, 2014Co-Authors: S Suganya, Jayarama Reddy Venugopal, Seeram Ramakrishna, Baddireddi Subhadra Lakshmi, V Giri R DevAbstract:Significant wound healing activity of Aloe vera (AV) and higher elastic strength of Silk fibroin (SF) along with mammalian cell compatibility makes AV and SF an attractive material for Tissue engineering. The purpose of the present work was to combine their unique properties, with the advantage of electrospinning to prepare a hybrid transdermal biomaterial for dermal substitutes. The physico-chemical characterization of the developed scaffold showed finer morphology expressing amino and esteric groups with improved hydrophilic properties and favorable tensile strain of 116% desirable for Skin Tissue engineering. Their biological response showed favorable fibroblast proliferation compared to control which almost increased linearly by (p < 0.01) 34.68% on day 3, (p < 0.01) 19.13% on day 6, and (p < 0.001) 97.86% on day 9 with higher expression of CMFDA, collagen and F-actin proteins. The obtained results prove that the nanofibrous scaffold with synergistic property of AV and SF would be a potential biomaterial for Skin Tissue regeneration.
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nanofibrous structured biomimetic strategies for Skin Tissue regeneration
Wound Repair and Regeneration, 2013Co-Authors: Venugopal Jayarama Reddy, Subramanian Sundarrajan, Sridhar Radhakrishnan, Rajeswari Ravichandran, Shayanti Mukherjee, Ramalingam Balamurugan, Seeram RamakrishnaAbstract:Mimicking porous topography of natural extracellular matrix is advantageous for successful regeneration of damaged Tissues or organs. Nanotechnology being one of the most promising and growing technology today shows an extremely huge potential in the field of Tissue engineering. Nanofibrous structures that mimic the native extracellular matrix and promote the adhesion of various cells are being developed as Tissue-engineered scaffolds for Skin, bone, vasculature, heart, cornea, nervous system, and other Tissues. A range of novel biocomposite materials has been developed to enhance the bioactive or therapeutic properties of these nanofibrous scaffolds via surface modifications, including the immobilization of functional cell-adhesive ligands and bioactive molecules such as drugs, enzymes, and cytokines. In Skin Tissue engineering, usage of allogeneic Skin is avoided to reestablish physiological continuity and also to address the challenge of curing acute and chronic wounds, which remains as the area of exploration with various biomimetic approaches. Two-dimensional, three-dimensional scaffolds and stem cells are presently used as dermal regeneration templates for the treatment of full-thickness Skin defects resulting from injuries and severe burns. The present review elaborates specifically on the fabrication of nanofibrous structured strategies for wound dressings, wound healing, and controlled release of growth factors for Skin Tissue regeneration.
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Polysaccharide nanofibrous scaffolds as a model for in vitro Skin Tissue regeneration
Journal of Materials Science: Materials in Medicine, 2012Co-Authors: Retheesh Krishnan, Jayarama Reddy Venugopal, R. Rajeswari, Subramanian Sundarrajan, Radhakrishnan Sridhar, M. Shayanti, Seeram RamakrishnaAbstract:Tissue engineering and nanotechnology have advanced a general strategy combining the cellular elements of living Tissue with sophisticated functional biocomposites to produce living structures of sufficient size and function at a low cost for clinical relevance. Xylan, a natural polysaccharide was electrospun along with polyvinyl alcohol (PVA) to produce Xylan/PVA nanofibers for Skin Tissue engineering. The Xylan/PVA glutaraldehyde (Glu) vapor cross-linked nanofibers were characterized by SEM, FT-IR, tensile testing and water contact angle measurements to analyze the morphology, functional groups, mechanical properties and wettability of the fibers for Skin Tissue regeneration. The cell-biomaterial interactions were studied by culturing human foreSkin fibroblasts on Xylan/PVA Glu vapor cross-linked and Xylan/PVA/Glu blend nanofibrous scaffolds. The observed results showed that the mechanical properties (72 %) and fibroblast proliferation significantly increased up to 23 % (P < 0.05) in 48 h Glu vapor cross-linked nanofibers compared to 24 h Glu vapor cross-linked Xylan/PVA nanofibers. The present study may prove that the natural biodegradable Xylan/PVA nanofibrous scaffolds have good potential for fibroblast adhesion, proliferation and cell matrix interactions relevant for Skin Tissue regeneration.
Mohammad Khorram - One of the best experts on this subject based on the ideXlab platform.
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Vascularization strategies for Skin Tissue engineering
Biomaterials Science, 2020Co-Authors: Armin Amirsadeghi, Arman Jafari, Loek J. Eggermont, Seyedeh-sara Hashemi, Sidi A. Bencherif, Mohammad KhorramAbstract:A number of challenges in Skin grafting for wound healing have drawn researchers to focus on Skin Tissue engineering as an alternative solution. The core idea of Tissue engineering is to use scaffolds, cells, and/or bioactive molecules to help the Skin to properly recover from injuries. Over the past decades, the field has significantly evolved, developing various strategies to accelerate and improve Skin regeneration. However, there are still several concerns that should be addressed. Among these limitations, vascularization is known as a critical challenge that needs thorough consideration. Delayed wound healing of large defects results in an insufficient vascular network and ultimately ischemia. Recent advances in the field of Tissue engineering paved the way to improve vascularization of Skin substitutes. Broadly, these solutions can be classified into two categories as (1) use of growth factors, reactive oxygen species-inducing nanoparticles, and stem cells to promote angiogenesis, and (2) in vitro or in vivo prevascularization of Skin grafts. This review summarizes the state-of-the-art approaches, their limitations, and highlights the latest advances in therapeutic vascularization strategies for Skin Tissue engineering.
Matthias W. Laschke - One of the best experts on this subject based on the ideXlab platform.
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Current and emerging vascularization strategies in Skin Tissue engineering
Critical Reviews in Biotechnology, 2016Co-Authors: Florian S. Frueh, Michael D. Menger, Nicole Lindenblatt, Pietro Giovanoli, Matthias W. LaschkeAbstract:Vascularization is a key process in Skin Tissue engineering, determining the biological function of artificial Skin implants. Hence, efficient vascularization strategies are a major prerequisite for the safe application of these implants in clinical practice. Current approaches include (i) modification of structural and physicochemical properties of dermal scaffolds, (ii) biological scaffold activation with growth factor-releasing systems or gene vectors, and (iii) generation of prevascularized Skin substitutes by seeding scaffolds with vessel-forming cells. These conventional approaches may be further supplemented by emerging strategies, such as transplantation of adipose Tissue-derived microvascular fragments, 3D bioprinting and microfluidics, miRNA modulation, cell sheet engineering, and fabrication of photosynthetic scaffolds. The successful translation of these vascularization strategies from bench to bedside may pave the way for a broad clinical implementation of Skin Tissue engineering.
Yoshihisa Aizu - One of the best experts on this subject based on the ideXlab platform.
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Construction of spectral reflectance database for estimation of absorption and scattering parameters in Skin Tissue
Diffuse Optical Spectroscopy and Imaging VII, 2019Co-Authors: Tomonori Yuasa, Tomoki Hashisaka, Yuji Masuda, Takaaki Maeda, Hideki Funamizu, Yoshihisa AizuAbstract:To measure optically physiological condition inside Skin Tissue, it is important to estimate optical parameters in Skin Tissue. In this study, we investigated a method to estimate absorption and scattering parameters in Skin Tissue from the spectral reflectance database constructed by using Monte Carlo simulation with a nine-layered Skin Tissue model.
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Quantitative evaluation on the depth and spread of light propagation in Skin Tissue using Monte Carlo simulation
Biomedical Imaging and Sensing Conference, 2017Co-Authors: Syoki Takahashi, Tomonori Yuasa, Takaaki Maeda, Hideki Funamizu, Yoshihisa AizuAbstract:Light propagation into human Skin Tissue is studied by using Monte Carlo simulation (MCS) with the multi-layered Skin Tissue model. In this study, we analyzed light propagation in various internal conditions of Skin Tissue by calculating photon fluence based on Monte Carlo simulation. And we examined a method for quantitative evaluation on the depth and spread of light propagation in Skin Tissue.
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Development of Skin Tissue phantom having a shape of sulcus cutis and crista cutis
Biomedical Imaging and Sensing Conference, 2017Co-Authors: Yutaro Nagamori, Tomonori Yuasa, Takaaki Maeda, Hideki Funamizu, Yoshihisa AizuAbstract:There are sulcus cutis and crista cutis on human Skin surface. It is known that these affect the light propagation in human Skin. To investigate it experimentally, it is desirable to reproduce sulcus cutis and crista cutis in Skin Tissue phantom. In this study, we made a prototype of Skin Tissue phantom having a shape of sulcus cutis and crista cutis, and investigated its optical properties and problems to be solved.
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Development of non-deterioration-type Skin Tissue phantom using silicone material
Optical Review, 2014Co-Authors: Saidatul Iva, Takaaki Maeda, Hideki Funamizu, Akihiro Tanabe, Yoshihisa AizuAbstract:We developed a new type of three-layered Skin Tissue phantom using silicone material. This phantom maintains its optical properties in a certain period of time, typically about three months, which is much longer than about 30 min with our previous agar-type phantom. Experiments on spectral reflectance measurements and color analysis indicate the possibility of silicone-type Skin Tissue phantom.
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monte carlo simulation of spectral reflectance using a multilayered Skin Tissue model
Optical Review, 2010Co-Authors: Takaaki Maeda, Naomi Arakawa, Motoji Takahashi, Yoshihisa AizuAbstract:A nine-layered Skin Tissue model is newly developed for the Monte Carlo simulation of spectral reflectance. The derivation of the necessary parameters for each of the nine layers in the simulation is presented, in which the parameters used in the conventional three-layered model are modified on the basis of some histological findings on Skin and reported examples. Using appropriate optical and geometrical parameters, simulated spectra can be produced that agree well with measured spectra. This approach provides a flexible means of spectral fitting to measured results and of estimating changes in the parameters of Skin Tissue.
Keith A. Seffen - One of the best experts on this subject based on the ideXlab platform.
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Thermally-induced change in the relaxation behavior of Skin Tissue.
Journal of biomechanical engineering, 2009Co-Authors: Keith A. SeffenAbstract:Skin biothermomechanics is highly interdisciplinary, involving bioheat transfer, burn damage, biomechanics, and physiology. Characterization of the thermomechanical behavior of Skin Tissue is of great importance and can contribute to a variety of medical applications. However, few quantitative studies have been conducted on the thermally-dependent mechanical properties of Skin Tissue. The aim of the present study is to experimentally examine the thermally-induced change in the relaxation behavior of Skin Tissue in both hyperthermal and hypothermic ranges. The results show that temperature has great influence on the stress-relaxation behavior of Skin Tissue under both hyperthermal and hypothermic temperatures; the quantitative relationship that has been found between temperature and the viscoelastic parameter (the elastic fraction or fractional energy dissipation) was temperature dependent, with greatest dissipation at high temperature levels.
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EFFECT OF THERMAL DAMAGE ON COMPRESSIVE BEHAVIOR OF Skin Tissue
Journal of Mechanics in Medicine and Biology, 2009Co-Authors: Keith A. SeffenAbstract:Biothermomechanics of Skin Tissue is highly interdisciplinary, involving bioheat transfer, burn damage, biomechanics and physiology. Characterization of the thermomechanical behavior of Skin Tissue is of great importance and can contribute to a variety of medical applications. However, few studies have attempted to address the influence of heat induced thermal damage on the mechanical properties of Skin Tissue. This paper presents the compressive behavior of pigSkin at different thermal damage levels and discusses the possible mechanisms of thermal damage–dependent compressive behavior of Skin. The results demonstrate that Skin stiffness decreases with increasing thermal damage degree and there exists strain rate sensitivity at different damage levels, caused mainly by hydration changes.
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Quantification of thermal damage in Skin Tissue
2008Co-Authors: T Wen, Keith A. SeffenAbstract:Skin thermal damage or Skin burns are the most commonly encountered type of trauma in civilian and military communities. Besides, advances in laser, microwave and similar technologies have led to recent developments of thermal treatments for disease and damage involving Skin Tissue, where the objective is to induce thermal damage precisely within targeted Tissue structures but without affecting the surrounding, healthy Tissue. Further, extended pain sensation induced by thermal damage has also brought great...
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Biothermomechanical behavior of Skin Tissue
Acta Mechanica Sinica, 2008Co-Authors: Keith A. SeffenAbstract:Advances in laser, microwave and similar technologies have led to recent developments of thermal treatments involving Skin Tissue. The effectiveness of these treatments is governed by the coupled thermal, mechanical, biological and neural responses of the affected Tissue: a favorable interaction results in a procedure with relatively little pain and no lasting side effects. Currently, even though each behavioral facet is to a certain extent established and understood, none exists to date in the interdisciplinary area. A highly interdisciplinary approach is required for studying the biothermomechanical behavior of Skin, involving bioheat transfer, biomechanics and physiology. A comprehensive literature review pertinent to the subject is presented in this paper, covering four subject areas: (a) Skin structure, (b) Skin bioheat transfer and thermal damage, (c) Skin biomechanics, and (d) Skin biothermomechanics. The major problems, issues, and topics for further studies are also outlined. This review finds that significant advances in each of these aspects have been achieved in recent years. Although focus is placed upon the biothermomechanical behavior of Skin Tissue, the fundamental concepts and methodologies reviewed in this paper may also be applicable for studying other soft Tissues.
