The Experts below are selected from a list of 327 Experts worldwide ranked by ideXlab platform
Irene M Gjermandsen - One of the best experts on this subject based on the ideXlab platform.
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Effect of pesticides on estrogen receptor transactivation in vitro: a comparison of stable transfected MVLN and transient transfected MCF-7 cells.
Molecular and cellular endocrinology, 2005Co-Authors: Eva C Bonefeld-jorgensen, Heidi T Grünfeld, Irene M GjermandsenAbstract:The estrogenic potential of four pesticides (endosulfan, prochloraz, tolchlofos-methyl and Propamocarb) was compared in parallel with 17beta-estradiol (E2) by reporter constructs in transient transfected MCF-7BUS and in stable transfected MVLN cells. Similar detection limit and half maximum effect concentration was determined for E2, whereas the maximum effect concentration of E2 was much higher in MCF-7BUS (10 nM) than in MVLN (150 pM), with the induced response being approximately six times the level in MVLN cells. Alone the four pesticides elicited the same relative response in the two bioassays, and similar data was obtained upon co-exposure with E2 for endosulfan and Propamocarb. In contrast to the transient MCF-7BUS system, endosulfan further increased the E2 induced response in MVLN cells, whereas Propamocarb did not induce the E2 response in MVLN cells as observed in MCF-7BUS cells. In conclusion, high agreement between the two reporter assays was observed, although some performance characteristics have to be considered.
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Effect of pesticides on estrogen receptor transactivation in vitro: a comparison of stable transfected MVLN and transient transfected MCF-7 cells.
Molecular and Cellular Endocrinology, 2005Co-Authors: Eva C Bonefeld-jorgensen, Heidi T Grünfeld, Irene M GjermandsenAbstract:Abstract The estrogenic potential of four pesticides (endosulfan, prochloraz, tolchlofos-methyl and Propamocarb) was compared in parallel with 17β-estradiol (E2) by reporter constructs in transient transfected MCF-7BUS and in stable transfected MVLN cells. Similar detection limit and half maximum effect concentration was determined for E2, whereas the maximum effect concentration of E2 was much higher in MCF-7BUS (10 nM) than in MVLN (150 pM), with the induced response being approximately six times the level in MVLN cells. Alone the four pesticides elicited the same relative response in the two bioassays, and similar data was obtained upon co-exposure with E2 for endosulfan and Propamocarb. In contrast to the transient MCF-7BUS system, endosulfan further increased the E2 induced response in MVLN cells, whereas Propamocarb did not induce the E2 response in MVLN cells as observed in MCF-7BUS cells. In conclusion, high agreement between the two reporter assays was observed, although some performance characteristics have to be considered.
Eva C Bonefeld-jorgensen - One of the best experts on this subject based on the ideXlab platform.
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Effect of pesticides on estrogen receptor transactivation in vitro: a comparison of stable transfected MVLN and transient transfected MCF-7 cells.
Molecular and cellular endocrinology, 2005Co-Authors: Eva C Bonefeld-jorgensen, Heidi T Grünfeld, Irene M GjermandsenAbstract:The estrogenic potential of four pesticides (endosulfan, prochloraz, tolchlofos-methyl and Propamocarb) was compared in parallel with 17beta-estradiol (E2) by reporter constructs in transient transfected MCF-7BUS and in stable transfected MVLN cells. Similar detection limit and half maximum effect concentration was determined for E2, whereas the maximum effect concentration of E2 was much higher in MCF-7BUS (10 nM) than in MVLN (150 pM), with the induced response being approximately six times the level in MVLN cells. Alone the four pesticides elicited the same relative response in the two bioassays, and similar data was obtained upon co-exposure with E2 for endosulfan and Propamocarb. In contrast to the transient MCF-7BUS system, endosulfan further increased the E2 induced response in MVLN cells, whereas Propamocarb did not induce the E2 response in MVLN cells as observed in MCF-7BUS cells. In conclusion, high agreement between the two reporter assays was observed, although some performance characteristics have to be considered.
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Effect of pesticides on estrogen receptor transactivation in vitro: a comparison of stable transfected MVLN and transient transfected MCF-7 cells.
Molecular and Cellular Endocrinology, 2005Co-Authors: Eva C Bonefeld-jorgensen, Heidi T Grünfeld, Irene M GjermandsenAbstract:Abstract The estrogenic potential of four pesticides (endosulfan, prochloraz, tolchlofos-methyl and Propamocarb) was compared in parallel with 17β-estradiol (E2) by reporter constructs in transient transfected MCF-7BUS and in stable transfected MVLN cells. Similar detection limit and half maximum effect concentration was determined for E2, whereas the maximum effect concentration of E2 was much higher in MCF-7BUS (10 nM) than in MVLN (150 pM), with the induced response being approximately six times the level in MVLN cells. Alone the four pesticides elicited the same relative response in the two bioassays, and similar data was obtained upon co-exposure with E2 for endosulfan and Propamocarb. In contrast to the transient MCF-7BUS system, endosulfan further increased the E2 induced response in MVLN cells, whereas Propamocarb did not induce the E2 response in MVLN cells as observed in MCF-7BUS cells. In conclusion, high agreement between the two reporter assays was observed, although some performance characteristics have to be considered.
Heidi T Grünfeld - One of the best experts on this subject based on the ideXlab platform.
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Effect of pesticides on estrogen receptor transactivation in vitro: a comparison of stable transfected MVLN and transient transfected MCF-7 cells.
Molecular and cellular endocrinology, 2005Co-Authors: Eva C Bonefeld-jorgensen, Heidi T Grünfeld, Irene M GjermandsenAbstract:The estrogenic potential of four pesticides (endosulfan, prochloraz, tolchlofos-methyl and Propamocarb) was compared in parallel with 17beta-estradiol (E2) by reporter constructs in transient transfected MCF-7BUS and in stable transfected MVLN cells. Similar detection limit and half maximum effect concentration was determined for E2, whereas the maximum effect concentration of E2 was much higher in MCF-7BUS (10 nM) than in MVLN (150 pM), with the induced response being approximately six times the level in MVLN cells. Alone the four pesticides elicited the same relative response in the two bioassays, and similar data was obtained upon co-exposure with E2 for endosulfan and Propamocarb. In contrast to the transient MCF-7BUS system, endosulfan further increased the E2 induced response in MVLN cells, whereas Propamocarb did not induce the E2 response in MVLN cells as observed in MCF-7BUS cells. In conclusion, high agreement between the two reporter assays was observed, although some performance characteristics have to be considered.
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Effect of pesticides on estrogen receptor transactivation in vitro: a comparison of stable transfected MVLN and transient transfected MCF-7 cells.
Molecular and Cellular Endocrinology, 2005Co-Authors: Eva C Bonefeld-jorgensen, Heidi T Grünfeld, Irene M GjermandsenAbstract:Abstract The estrogenic potential of four pesticides (endosulfan, prochloraz, tolchlofos-methyl and Propamocarb) was compared in parallel with 17β-estradiol (E2) by reporter constructs in transient transfected MCF-7BUS and in stable transfected MVLN cells. Similar detection limit and half maximum effect concentration was determined for E2, whereas the maximum effect concentration of E2 was much higher in MCF-7BUS (10 nM) than in MVLN (150 pM), with the induced response being approximately six times the level in MVLN cells. Alone the four pesticides elicited the same relative response in the two bioassays, and similar data was obtained upon co-exposure with E2 for endosulfan and Propamocarb. In contrast to the transient MCF-7BUS system, endosulfan further increased the E2 induced response in MVLN cells, whereas Propamocarb did not induce the E2 response in MVLN cells as observed in MCF-7BUS cells. In conclusion, high agreement between the two reporter assays was observed, although some performance characteristics have to be considered.
Liu Yana - One of the best experts on this subject based on the ideXlab platform.
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Residues and dissipation of Propamocarb in tomatoes and soil using UPLC-MS / MS
Environmental Chemistry, 2015Co-Authors: Liu YanaAbstract:The dissipation of Propamocarb in tomato and soil was studied to formulate a scientific basis for the acceptable dose and the pre-harvest interval. Dissipation experiments were conducted by applying Propamocarb once on tomato and soil with hole application at 90 g·ha- 1after fruit enlargement. The samples were collected randomly from each plot at different time intervals i. e,2h,1,2,4,7,14 days after the application. Dispersive solid phase extraction combined to ultrahigh performance liquid chromatography- tandemmass spectrometry( UPLC-MS / MS) method was used to determine the Propamocarb residue in tomato and soil. Recovery test was processed in three spiked levels of 0. 02,0. 20 and 2. 00 mg·kg- 1. The results showed that the within-day average recovery rate of Propamocarb in tomato and soil were 83. 9% —104. 6%,the within-day RSDs were1. 0% —5. 5%; the between-day average recovery rate were 84. 3% —108. 9%,the between-dayRSDs were 1. 4% —4. 9%. The correlation coefficient of Propamocarb in the range of 1. 0—200. 0μg·L- 1was R2 0. 9992,LOQ were 0. 02 mg·kg- 1. The actual samples and field experimental samples were detected by using this method,the results showed that the degradation half-life of Propamocarb in tomato and soil were 2. 4—4. 7 d and 1. 1—1. 5 d,respectively. The residues were less than max residue limits( MRLs). The Propamocarb agent( 72. 2%) applied with hole application can be considered safe after more than 2 h in the application to the pre-harvest intervals.
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residues and dissipation of Propamocarb in tomatoes and soil using uplc ms ms
Environmental Chemistry, 2015Co-Authors: Liu YanaAbstract:The dissipation of Propamocarb in tomato and soil was studied to formulate a scientific basis for the acceptable dose and the pre-harvest interval. Dissipation experiments were conducted by applying Propamocarb once on tomato and soil with hole application at 90 g·ha- 1after fruit enlargement. The samples were collected randomly from each plot at different time intervals i. e,2h,1,2,4,7,14 days after the application. Dispersive solid phase extraction combined to ultrahigh performance liquid chromatography- tandemmass spectrometry( UPLC-MS / MS) method was used to determine the Propamocarb residue in tomato and soil. Recovery test was processed in three spiked levels of 0. 02,0. 20 and 2. 00 mg·kg- 1. The results showed that the within-day average recovery rate of Propamocarb in tomato and soil were 83. 9% —104. 6%,the within-day RSDs were1. 0% —5. 5%; the between-day average recovery rate were 84. 3% —108. 9%,the between-dayRSDs were 1. 4% —4. 9%. The correlation coefficient of Propamocarb in the range of 1. 0—200. 0μg·L- 1was R2 0. 9992,LOQ were 0. 02 mg·kg- 1. The actual samples and field experimental samples were detected by using this method,the results showed that the degradation half-life of Propamocarb in tomato and soil were 2. 4—4. 7 d and 1. 1—1. 5 d,respectively. The residues were less than max residue limits( MRLs). The Propamocarb agent( 72. 2%) applied with hole application can be considered safe after more than 2 h in the application to the pre-harvest intervals.
Balwinder Singh - One of the best experts on this subject based on the ideXlab platform.
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analysis of fluopicolide and Propamocarb residues on tomato and soil using quechers sample preparation method in combination with glc and gcms
Food Analytical Methods, 2014Co-Authors: S K Sahoo, Kousik Mandal, Rajinder Kumar, Balwinder SinghAbstract:Persistence of fluopicolide and Propamocarb in tomato was studied following three applications of a combination formulation of Infinito 68.75 SC (fluopicolide 6.25 % + Propamocarb 62.5 %) at 1500 and 3000 mL ha−1 by 7 days interval, starting the spray at fruit development stage. QuEChERS method included extraction of sample with ethyl acetate and cleanup of dispersive solid-phase extraction was used for the determination of fluopicolide and Propamocarb residues on tomato and soil. Residues of fluopicolide and Propamocarb in tomato were estimated by gas–liquid chromatography and gas chromatograph–mass spectrometry, respectively. Half-lives for fluopicolide were found to be 2.58 and 2.31 days, whereas for Propamocarb these values were observed to be 1.49 and 2.08 days at single and double the application rates, respectively. Residues of fluopicolide dissipated below its limit of quantification (LOQ) of 0.01 mg kg−1 after 7 and 10 days at single and double the application dosage, respectively. Similarly, residues of Propamocarb took 5 and 7 days to reach LOQ of 0.10 mg kg−1, at single and double dosages, respectively. Soil samples collected after 15 days of the last application did not show the presence of fluopicolide and Propamocarb at their detection limit of 0.01 and 0.10 mg kg−1, respectively. The initial deposit of residues of Propamocarb and fluopicolide for both the dosages were below the prescribed codex maximum residue limit values of 2 and 1 mg kg−1, respectively. Therefore, a 1-day waiting period was suggested to reduce human health risks before consumption of tomato fruits.
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Development and Validation of QuEChERS Method for Estimation of Propamocarb Residues in Tomato (Lycopersicon esculentum Mill) and Soil
American Journal of Analytical Chemistry, 2011Co-Authors: S K Sahoo, Raminderjit Singh Battu, Balwinder SinghAbstract:An easy, simple and efficient analytical method was standardized and validated for the estimation of residues of Propamocarb in tomato and soil. QuEChERS method included extraction of the sample with ethyl acetate and cleanup by treatment with PSA and graphatised carbon. Final clear extracts of ethyl acetate were concentrated under vacuum to almost dryness and reconstituted into hexane. The residues of Propamocarbwere estimated using gas chromatograph-mass spectrometry (GC-MS). Propamocarb presented a distinct peak at retention time of 8.962 min. Consistent recoveries of Propamocarb ranging from 87 to 92 percent were observed when samples were spiked at 0.10, 0.50 and 1.00 mg·kg–1 levels. The limit of quantification (LOQ) of this method was determined to be 0.10 mg·kg–1.
