The Experts below are selected from a list of 282 Experts worldwide ranked by ideXlab platform
Yihui Chen - One of the best experts on this subject based on the ideXlab platform.
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Inhibitory effect of Propyl Gallate on pulp breakdown of longan fruit and its relationship with ROS metabolism
Postharvest Biology and Technology, 2020Co-Authors: Yixiong Lin, Hetong Lin, Yihui Chen, Zhongqi Fan, Hui Wang, Mengshi Lin, Yen-con Hung, Yifen LinAbstract:Abstract An excessive ROS accumulation in harvested longan (Dimocarpus longan Lour.) fruit could promote the pulp breakdown and lower the fruit quality. To solve this problem, the influence of Propyl Gallate, one kind of ROS scavenger, on the ROS metabolism in pulp of postharvest longans and its connection with the breakdown occurrence of longan pulp were investigated. Contrasted to control samples, the Propyl Gallate-treated group manifested a lower index of pulp breakdown, MDA content, and production rate of O2−., but higher gene expression levels of DlSOD, DlCAT and DlAPX, higher SOD, CAT and APX activities, higher levels of AsA, flavonoid, GSH and total phenolics, and higher ability of scavenging free radicals. These results revealed that Propyl Gallate inhibited longan pulp breakdown occurrence, which was because Propyl Gallate enhanced the ROS scavenging ability and reduced ROS generation and accumulation in longan pulp. These activities involved alleviating the oxidative damage of cell membrane and the peroxidation of membrane lipid in longan pulp, which maintained the structural integrity of longan pulp cell membrane and suppressed the pulp breakdown occurrence in postharvest longan fruit.
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application of Propyl Gallate alleviates pericarp browning in harvested longan fruit by modulating metabolisms of respiration and energy
Food Chemistry, 2018Co-Authors: Yihui Chen, Hui WangAbstract:Abstract Effects of Propyl Gallate on metabolisms of respiration and energy of harvested ‘Fuyan’ longans and its relationship to pericarp browning were investigated. Compared to control longans, Propyl Gallate could reduce ascorbic acid oxidase (AAO) activity, lower cytochrome C oxidase (CCO) activity during early-storage and mid-storage, increase NADK activity, elevate contents of NADP and NADPH, decrease contents of NAD and NADH, in addition, lower the decreases of ATP content and energy charge (E.C.), increase activities of mitochondrial H+-ATPase, Ca2+-ATPase and Mg2+-ATPase during early-storage and mid-storage. Above results suggested that Propyl Gallate-retarded browning development in pericarp of harvested longans was resulted from decreases in activities of respiratory terminal oxidases like CCO and AAO, increase in proportion of pentose phosphate pathway (PPP) to Embden-Meyerhof pathway (EMP) and tricarboxylic acid (TCA) cycle, and maintenance of mitochondrial integrity via retaining higher levels of ATP content and energy charge, as well as higher activities of mitochondrial ATPase.
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Inhibitory effects of Propyl Gallate on membrane lipids metabolism and its relation to increasing storability of harvested longan fruit
Food chemistry, 2016Co-Authors: Yifen Lin, Hetong Lin, Yihui Chen, Yixiong Lin, John Shi, Hui WangAbstract:Effects of Propyl Gallate on membrane lipids metabolism and its relation to storability of harvested longan fruits were studied. The results showed that the Propyl Gallate-treated longans maintained lower activities of pericarp phospholipase D (PLD), lipase and lipoxygenase (LOX) than those in control fruits. Such treatments could maintain higher levels of pericarp unsaturated fatty acids (USFAs), higher pericarp indices of unsaturated fatty acids (IUFA), and higher pericarp ratio of unsaturated fatty acids to saturated fatty acids (U/S) than those in control fruits. Furthermore, Propyl Gallate also delayed color changes of pericarp in the harvested longans. Therefore, the postharvest treatments of longan fruits with Propyl Gallate for increasing storability of longan fruits might be explained by a decrease in activities of PLD, lipase and LOX, and an the increased unsaturation of fatty acids, which could delay membrane lipids metabolism and maintain cell membrane characteristics.
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inhibitory effects of Propyl Gallate on browning and its relationship to active oxygen metabolism in pericarp of harvested longan fruit
Lwt - Food Science and Technology, 2015Co-Authors: Shen Zhang, Yihui ChenAbstract:The inhibitory effects of Propyl Gallate, an active oxygen scavenger, on browning and active oxygen metabolism in the pericarp of harvested ‘Fuyan’ longan fruit were investigated. The results indicated a lower browning index in the pericarp of Propyl Gallate-treated fruit when compared with the control fruit. The fruit treated with Propyl Gallate displayed a decreased rate of superoxide anion (O2·¯) production, maintained higher activities of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX), had higher contents of ascorbic acid (AsA), glutathione (GSH) and carotenoid, and had lower malondialdehyde (MDA) content. These results give convincing evidence that the retardation of pericarp browning of harvested longan fruit is due to an increased capacity to scavenge active oxygen species and decrease the accumulation of O2·¯. Controlling the level of active oxygen species will reduce membrane lipid peroxidation, maintain cellular membrane structure and the integrity of compartmentalization. Preventing polyphenol oxidase (PPO) and peroxidase (POD) from contacting their respective phenolic substrate will reduce the occurrence of enzymatic browning in the pericarp of harvested longan fruit. It is possible that Propyl Gallate treatment could be a feasible technique for controlling pericarp browning and extend the storage life of harvested longan fruit.
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Inhibitory effects of Propyl Gallate on tyrosinase and its application in controlling pericarp browning of harvested longan fruits.
Journal of agricultural and food chemistry, 2013Co-Authors: Yifen Lin, Hetong Lin, Xuan Liu, Yihui Chen, Shen Zhang, Qing-xi ChenAbstract:Tyrosinase (EC 1.14.18.1), also known as polyphenol oxidase (PPO), is a key enzyme in pigment biosynthesis of organisms. The inhibitory effects of Propyl Gallate on the activity of mushroom tyrosinase and effects of Propyl Gallate on pericarp browning of harvested longan fruits in relation to phenolic metabolism were investigated. The results showed that Propyl Gallate could potently inhibit diphenolase activity of tyrosinase. The inhibitor concentration leading to 50% activity lost (IC50) was determined to be 0.685 mM. Kinetic analyses showed that Propyl Gallate was a reversible and mixed type inhibitor on this enzyme. The inhibition constants (K(IS) and K(I)) were determined to be 2.135 and 0.661 mM, respectively. Furthermore, the results also showed that Propyl Gallate treatment inhibited activities of PPO and POD in pericarp of harvested longan fruits, and maintained higher contents of total phenol and flavonoid of longan pericarp. Moreover, Propyl Gallate treatment also delayed the increases of browning index and browning degree in pericarp of harvested longan fruits. Therefore, application of Propyl Gallate may be a promising method for inhibiting tyrosinase activity, controlling pericarp browning, and extending shelf life of harvested longan fruits.
Adelar Bracht - One of the best experts on this subject based on the ideXlab platform.
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Kinetics of the transformation of n-Propyl Gallate and structural analogs in the perfused rat liver.
Toxicology and applied pharmacology, 2013Co-Authors: Gabrielle Jacklin Eler, Rosane Marina Peralta, Israel Souza Santos, Amarilis Giaretta De Moraes, Márcio Shigueaki Mito, Jurandir Fernando Comar, Adelar BrachtAbstract:n-Propyl Gallate and its analogs are used in foods and other products to prevent oxidation. In the liver the compound exerts several harmful effects, especially gluconeogenesis inhibition. The mode of transport and distribution of n-Propyl Gallate and its kinetics of biotransformation have not yet been investigated. To fill this gap the transformation, transport and distribution of n-Propyl Gallate and two analogs were investigated in the rat liver. Isolated perfused rat liver was used. n-Propyl Gallate, methyl Gallate, n-octyl Gallate and transformation products were quantified by high pressure-liquid chromatography coupled to fluorescence detection. The interactions of n-Propyl Gallate and analogs with the liver presented three main characteristics: (1) the hydrolytic release of gallic acid from n-Propyl Gallate and methyl Gallate was very fast compared with the subsequent transformations of the gallic acid moiety; (2) transport of the esters was very fast and flow-limited in contrast to the slow and barrier-limited transport of gallic acid; (3) the apparent distribution volume of n-Propyl Gallate, but probably also of methyl Gallate and n-octyl Gallate, greatly exceeded the water space in the liver, contrary to the gallic acid space which is smaller than the water space. It can be concluded that at low portal concentrations (< 50 μM) the gallic acid esters are 100% extracted during a single passage through the liver, releasing mainly gallic acid into the systemic circulation. For the latter a considerable time is required until complete biotransformation. The exposure of the liver to the esters, however, is quite prolonged due to extensive intracellular binding.
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The action of n-Propyl Gallate on gluconeogenesis and oxygen uptake in the rat liver.
Chemico-biological interactions, 2009Co-Authors: Gabrielle Jacklin Eler, Rosane Marina Peralta, Adelar BrachtAbstract:Abstract In the present study the metabolic actions of n-Propyl Gallate on hepatic gluconeogenesis, oxygen uptake and related parameters were investigated. Experiments were done in the isolated perfused rat liver. n-Propyl Gallate inhibited gluconeogenesis and stimulated oxygen uptake at concentrations up to 200 μM. The inhibitory effects on lactate gluconeogenesis (ED50 86.4 μM) and alanine gluconeogenesis were considerably more pronounced than those on glycerol and fructose gluconeogenesis. n-Propyl Gallate also stimulated oxygen uptake in both the mitochondrial (63%) and microsomal (37%) electron transport chains. The first one is due mainly to the oxidation of n-propanol, as a metabolite of the first step of n-Propyl Gallate transformation. The second one results from a direct stimulation of the microsomal electron transport chain. n-Propyl Gallate inhibited pyruvate carboxylation (ED50 142.2 μM) in consequence of an inhibition of pyruvate transport into the mitochondria an effect not found for gallic acid. This is probably the main cause for glucose output inhibition. Secondary causes are (1) deviation of intermediates for the production of NADPH to be used in microsomal electron transport; (2) deviation of glucose 6-phosphate for glucuronidation reactions; (3) gluconeogenesis inhibition by n-propanol, produced intracellularly from n-Propyl Gallate. Inhibition of mitochondrial energy metabolism is not significant in the range up to 200 μM, as indicated by the very small effect on the cellular ATP levels (5% decreased). n-Propyl Gallate can be considered a kind of metabolic effector, whose actions on the liver metabolism are relatively mild although they can become harmful for the organ and the whole organism at high doses and concentrations.
Hui Wang - One of the best experts on this subject based on the ideXlab platform.
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Inhibitory effect of Propyl Gallate on pulp breakdown of longan fruit and its relationship with ROS metabolism
Postharvest Biology and Technology, 2020Co-Authors: Yixiong Lin, Hetong Lin, Yihui Chen, Zhongqi Fan, Hui Wang, Mengshi Lin, Yen-con Hung, Yifen LinAbstract:Abstract An excessive ROS accumulation in harvested longan (Dimocarpus longan Lour.) fruit could promote the pulp breakdown and lower the fruit quality. To solve this problem, the influence of Propyl Gallate, one kind of ROS scavenger, on the ROS metabolism in pulp of postharvest longans and its connection with the breakdown occurrence of longan pulp were investigated. Contrasted to control samples, the Propyl Gallate-treated group manifested a lower index of pulp breakdown, MDA content, and production rate of O2−., but higher gene expression levels of DlSOD, DlCAT and DlAPX, higher SOD, CAT and APX activities, higher levels of AsA, flavonoid, GSH and total phenolics, and higher ability of scavenging free radicals. These results revealed that Propyl Gallate inhibited longan pulp breakdown occurrence, which was because Propyl Gallate enhanced the ROS scavenging ability and reduced ROS generation and accumulation in longan pulp. These activities involved alleviating the oxidative damage of cell membrane and the peroxidation of membrane lipid in longan pulp, which maintained the structural integrity of longan pulp cell membrane and suppressed the pulp breakdown occurrence in postharvest longan fruit.
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application of Propyl Gallate alleviates pericarp browning in harvested longan fruit by modulating metabolisms of respiration and energy
Food Chemistry, 2018Co-Authors: Yihui Chen, Hui WangAbstract:Abstract Effects of Propyl Gallate on metabolisms of respiration and energy of harvested ‘Fuyan’ longans and its relationship to pericarp browning were investigated. Compared to control longans, Propyl Gallate could reduce ascorbic acid oxidase (AAO) activity, lower cytochrome C oxidase (CCO) activity during early-storage and mid-storage, increase NADK activity, elevate contents of NADP and NADPH, decrease contents of NAD and NADH, in addition, lower the decreases of ATP content and energy charge (E.C.), increase activities of mitochondrial H+-ATPase, Ca2+-ATPase and Mg2+-ATPase during early-storage and mid-storage. Above results suggested that Propyl Gallate-retarded browning development in pericarp of harvested longans was resulted from decreases in activities of respiratory terminal oxidases like CCO and AAO, increase in proportion of pentose phosphate pathway (PPP) to Embden-Meyerhof pathway (EMP) and tricarboxylic acid (TCA) cycle, and maintenance of mitochondrial integrity via retaining higher levels of ATP content and energy charge, as well as higher activities of mitochondrial ATPase.
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Inhibitory effects of Propyl Gallate on membrane lipids metabolism and its relation to increasing storability of harvested longan fruit
Food chemistry, 2016Co-Authors: Yifen Lin, Hetong Lin, Yihui Chen, Yixiong Lin, John Shi, Hui WangAbstract:Effects of Propyl Gallate on membrane lipids metabolism and its relation to storability of harvested longan fruits were studied. The results showed that the Propyl Gallate-treated longans maintained lower activities of pericarp phospholipase D (PLD), lipase and lipoxygenase (LOX) than those in control fruits. Such treatments could maintain higher levels of pericarp unsaturated fatty acids (USFAs), higher pericarp indices of unsaturated fatty acids (IUFA), and higher pericarp ratio of unsaturated fatty acids to saturated fatty acids (U/S) than those in control fruits. Furthermore, Propyl Gallate also delayed color changes of pericarp in the harvested longans. Therefore, the postharvest treatments of longan fruits with Propyl Gallate for increasing storability of longan fruits might be explained by a decrease in activities of PLD, lipase and LOX, and an the increased unsaturation of fatty acids, which could delay membrane lipids metabolism and maintain cell membrane characteristics.
Yifen Lin - One of the best experts on this subject based on the ideXlab platform.
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Inhibitory effect of Propyl Gallate on pulp breakdown of longan fruit and its relationship with ROS metabolism
Postharvest Biology and Technology, 2020Co-Authors: Yixiong Lin, Hetong Lin, Yihui Chen, Zhongqi Fan, Hui Wang, Mengshi Lin, Yen-con Hung, Yifen LinAbstract:Abstract An excessive ROS accumulation in harvested longan (Dimocarpus longan Lour.) fruit could promote the pulp breakdown and lower the fruit quality. To solve this problem, the influence of Propyl Gallate, one kind of ROS scavenger, on the ROS metabolism in pulp of postharvest longans and its connection with the breakdown occurrence of longan pulp were investigated. Contrasted to control samples, the Propyl Gallate-treated group manifested a lower index of pulp breakdown, MDA content, and production rate of O2−., but higher gene expression levels of DlSOD, DlCAT and DlAPX, higher SOD, CAT and APX activities, higher levels of AsA, flavonoid, GSH and total phenolics, and higher ability of scavenging free radicals. These results revealed that Propyl Gallate inhibited longan pulp breakdown occurrence, which was because Propyl Gallate enhanced the ROS scavenging ability and reduced ROS generation and accumulation in longan pulp. These activities involved alleviating the oxidative damage of cell membrane and the peroxidation of membrane lipid in longan pulp, which maintained the structural integrity of longan pulp cell membrane and suppressed the pulp breakdown occurrence in postharvest longan fruit.
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Inhibitory effects of Propyl Gallate on membrane lipids metabolism and its relation to increasing storability of harvested longan fruit
Food chemistry, 2016Co-Authors: Yifen Lin, Hetong Lin, Yihui Chen, Yixiong Lin, John Shi, Hui WangAbstract:Effects of Propyl Gallate on membrane lipids metabolism and its relation to storability of harvested longan fruits were studied. The results showed that the Propyl Gallate-treated longans maintained lower activities of pericarp phospholipase D (PLD), lipase and lipoxygenase (LOX) than those in control fruits. Such treatments could maintain higher levels of pericarp unsaturated fatty acids (USFAs), higher pericarp indices of unsaturated fatty acids (IUFA), and higher pericarp ratio of unsaturated fatty acids to saturated fatty acids (U/S) than those in control fruits. Furthermore, Propyl Gallate also delayed color changes of pericarp in the harvested longans. Therefore, the postharvest treatments of longan fruits with Propyl Gallate for increasing storability of longan fruits might be explained by a decrease in activities of PLD, lipase and LOX, and an the increased unsaturation of fatty acids, which could delay membrane lipids metabolism and maintain cell membrane characteristics.
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Inhibitory effects of Propyl Gallate on tyrosinase and its application in controlling pericarp browning of harvested longan fruits.
Journal of agricultural and food chemistry, 2013Co-Authors: Yifen Lin, Hetong Lin, Xuan Liu, Yihui Chen, Shen Zhang, Qing-xi ChenAbstract:Tyrosinase (EC 1.14.18.1), also known as polyphenol oxidase (PPO), is a key enzyme in pigment biosynthesis of organisms. The inhibitory effects of Propyl Gallate on the activity of mushroom tyrosinase and effects of Propyl Gallate on pericarp browning of harvested longan fruits in relation to phenolic metabolism were investigated. The results showed that Propyl Gallate could potently inhibit diphenolase activity of tyrosinase. The inhibitor concentration leading to 50% activity lost (IC50) was determined to be 0.685 mM. Kinetic analyses showed that Propyl Gallate was a reversible and mixed type inhibitor on this enzyme. The inhibition constants (K(IS) and K(I)) were determined to be 2.135 and 0.661 mM, respectively. Furthermore, the results also showed that Propyl Gallate treatment inhibited activities of PPO and POD in pericarp of harvested longan fruits, and maintained higher contents of total phenol and flavonoid of longan pericarp. Moreover, Propyl Gallate treatment also delayed the increases of browning index and browning degree in pericarp of harvested longan fruits. Therefore, application of Propyl Gallate may be a promising method for inhibiting tyrosinase activity, controlling pericarp browning, and extending shelf life of harvested longan fruits.
Shen Zhang - One of the best experts on this subject based on the ideXlab platform.
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inhibitory effects of Propyl Gallate on browning and its relationship to active oxygen metabolism in pericarp of harvested longan fruit
Lwt - Food Science and Technology, 2015Co-Authors: Shen Zhang, Yihui ChenAbstract:The inhibitory effects of Propyl Gallate, an active oxygen scavenger, on browning and active oxygen metabolism in the pericarp of harvested ‘Fuyan’ longan fruit were investigated. The results indicated a lower browning index in the pericarp of Propyl Gallate-treated fruit when compared with the control fruit. The fruit treated with Propyl Gallate displayed a decreased rate of superoxide anion (O2·¯) production, maintained higher activities of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX), had higher contents of ascorbic acid (AsA), glutathione (GSH) and carotenoid, and had lower malondialdehyde (MDA) content. These results give convincing evidence that the retardation of pericarp browning of harvested longan fruit is due to an increased capacity to scavenge active oxygen species and decrease the accumulation of O2·¯. Controlling the level of active oxygen species will reduce membrane lipid peroxidation, maintain cellular membrane structure and the integrity of compartmentalization. Preventing polyphenol oxidase (PPO) and peroxidase (POD) from contacting their respective phenolic substrate will reduce the occurrence of enzymatic browning in the pericarp of harvested longan fruit. It is possible that Propyl Gallate treatment could be a feasible technique for controlling pericarp browning and extend the storage life of harvested longan fruit.
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Inhibitory effects of Propyl Gallate on tyrosinase and its application in controlling pericarp browning of harvested longan fruits.
Journal of agricultural and food chemistry, 2013Co-Authors: Yifen Lin, Hetong Lin, Xuan Liu, Yihui Chen, Shen Zhang, Qing-xi ChenAbstract:Tyrosinase (EC 1.14.18.1), also known as polyphenol oxidase (PPO), is a key enzyme in pigment biosynthesis of organisms. The inhibitory effects of Propyl Gallate on the activity of mushroom tyrosinase and effects of Propyl Gallate on pericarp browning of harvested longan fruits in relation to phenolic metabolism were investigated. The results showed that Propyl Gallate could potently inhibit diphenolase activity of tyrosinase. The inhibitor concentration leading to 50% activity lost (IC50) was determined to be 0.685 mM. Kinetic analyses showed that Propyl Gallate was a reversible and mixed type inhibitor on this enzyme. The inhibition constants (K(IS) and K(I)) were determined to be 2.135 and 0.661 mM, respectively. Furthermore, the results also showed that Propyl Gallate treatment inhibited activities of PPO and POD in pericarp of harvested longan fruits, and maintained higher contents of total phenol and flavonoid of longan pericarp. Moreover, Propyl Gallate treatment also delayed the increases of browning index and browning degree in pericarp of harvested longan fruits. Therefore, application of Propyl Gallate may be a promising method for inhibiting tyrosinase activity, controlling pericarp browning, and extending shelf life of harvested longan fruits.
