The Experts below are selected from a list of 26898 Experts worldwide ranked by ideXlab platform
Feng Chen - One of the best experts on this subject based on the ideXlab platform.
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sonochemical synthesis of hydroxyapatite nanoflowers using creatine phosphate disodium salt as an organic phosphorus source and their application in Protein adsorption
RSC Advances, 2016Co-Authors: Yingjie Zhu, Tuanwei Sun, Yingying Jiang, Yonggang Zhang, Feng ChenAbstract:In this paper, the one-step rapid synthesis of hydroxyapatite nanoflowers (HAFs) using creatine phosphate disodium salt as an organic phosphorus source by the sonochemical method is reported. The HAFs with diameters of about 300 nm are formed by self-assembly of hydroxyapatite nanosheets with thicknesses of less than 10 nm. The as-prepared samples are characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Brunauer–Emmett–Teller (BET) nitrogen sorptometry, X-ray powder diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy and inductively coupled plasma (ICP) optical emission spectroscopy. The MTT tests show that the as-prepared HAFs exhibit essentially inappreciable toxicity to MC-3T3 osteoblast cells, indicating that the HAFs have an excellent cytocompatibility. Moreover, the as-prepared HAFs show a relatively high Protein adsorption ability when using hemoglobin (Hb) as a model Protein. Thus, the as-prepared HAFs are promising for applications in various biomedical fields such as Protein/Drug Delivery.
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yolk shell porous microspheres of calcium phosphate prepared by using calcium l lactate and adenosine 5 triphosphate disodium salt application in Protein Drug Delivery
Chemistry: A European Journal, 2015Co-Authors: Guanjun Ding, Tuanwei Sun, Yingjie Zhu, Feng ChenAbstract:A facile and environmentally friendly approach has been developed to prepare yolk-shell porous microspheres of calcium phosphate by using calcium L-lactate pentahydrate (CL) as the calcium source and adenosine 5′-triphosphate disodium salt (ATP) as the phosphate source through the microwave-assisted hydrothermal method. The effects of the concentration of CL, the microwave hydrothermal temperature, and the time on the morphology and crystal phase of the product are investigated. The possible formation mechanism of yolk-shell porous microspheres of calcium phosphate is proposed. Hemoglobin from bovine red cells (Hb) and ibuprofen (IBU) are used to explore the application potential of yolk-shell porous microspheres of calcium phosphate in Protein/Drug loading and Delivery. The experimental results indicate that the as-prepared yolk-shell porous microspheres of calcium phosphate have relatively high Protein/Drug loading capacity, sustained Protein/Drug release, favorable pH-responsive release behavior, and a high biocompatibility in the cytotoxicity test. Therefore, the yolk-shell porous microspheres of calcium phosphate have promising applications in various biomedical fields such as Protein/Drug Delivery.
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nanosheet assembled hierarchical nanostructures of hydroxyapatite surfactant free microwave hydrothermal rapid synthesis Protein dna adsorption and ph controlled release
CrystEngComm, 2013Co-Authors: Xinyu Zhao, Yingjie Zhu, Feng ChenAbstract:In this paper, a surfactant-free rapid microwave-assisted hydrothermal synthesis of hydroxyapatite nanosheet-assembled flower-like hierarchical nanostructures (NFHNs) is reported. The effects of the experimental conditions on the morphology and crystal phase of the product are investigated. A possible formation mechanism of hydroxyapatite NFHNs is proposed. The morphology of the product can vary from flower-like to polyhedra by adjusting the microwave heating temperature. The Protein and DNA adsorption properties of the as-prepared hydroxyapatite NFHNs are studied. The loading capacities of the as-prepared hydroxyapatite NFHNs for bovine serum albumin (BSA), hemoglobin (Hb) and fish sperm DNA are determined to be 165, 164 and 112 mg g−1, respectively. The Protein release process is conducted at different pH values (pH 7.2, 5.5 and 4.8) in phosphate buffer saline (PBS), and the pH-controlled Protein release behavior has been found. Thus, the as-prepared hydroxyapatite NFHNs are promising for Protein Drug Delivery applications.
Cui Tang - One of the best experts on this subject based on the ideXlab platform.
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chitosan graft copolymer nanoparticles for oral Protein Drug Delivery preparation and characterization
Biomacromolecules, 2006Co-Authors: Feng Qian, Jieying Ding, Cui TangAbstract:Several novel functionalized graft copolymer nanoparticles consisting of chitosan (CS) and the monomer methyl methacrylate (MMA), N-dimethylaminoethyl methacrylate hydrochloride (DMAEMC), and N-trimethylaminoethyl methacrylate chloride (TMAEMC), which show a higher solubility than chitosan in a broader pH range, have been prepared by free radical polymerization. The nanoparticles were characterized in terms of particle size, zeta potential, TEM, and FT-IR. These nanoparticles were 150−280 nm in size and carried obvious positive surface charges. Protein-loaded nanoparticles were prepared, and their maximal encapsulation efficiency was up to 100%. In vitro release showed that these nanoparticles provided an initial burst release followed by a slowly sustained release for more than 24 h. These graft copolymer nanoparticles enhanced the absorption and improved the bioavailability of insulin via the gastrointestinal (GI) tract of normal male Sprague−Dawley (SD) strain rats to a greater extent than that of the ...
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chitosan graft copolymer nanoparticles for oral Protein Drug Delivery preparation and characterization
Biomacromolecules, 2006Co-Authors: Feng Qian, Jieying Ding, Cui TangAbstract:Several novel functionalized graft copolymer nanoparticles consisting of chitosan (CS) and the monomer methyl methacrylate (MMA), N-dimethylaminoethyl methacrylate hydrochloride (DMAEMC), and N-trimethylaminoethyl methacrylate chloride (TMAEMC), which show a higher solubility than chitosan in a broader pH range, have been prepared by free radical polymerization. The nanoparticles were characterized in terms of particle size, zeta potential, TEM, and FT-IR. These nanoparticles were 150−280 nm in size and carried obvious positive surface charges. Protein-loaded nanoparticles were prepared, and their maximal encapsulation efficiency was up to 100%. In vitro release showed that these nanoparticles provided an initial burst release followed by a slowly sustained release for more than 24 h. These graft copolymer nanoparticles enhanced the absorption and improved the bioavailability of insulin via the gastrointestinal (GI) tract of normal male Sprague−Dawley (SD) strain rats to a greater extent than that of the ...
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chitosan graft copolymer nanoparticles for oral Protein Drug Delivery preparation and characterization
Biomacromolecules, 2006Co-Authors: Feng Qian, Jieying Ding, Cui Tang, Fuying Cui, Chunhua YinAbstract:Several novel functionalized graft copolymer nanoparticles consisting of chitosan (CS) and the monomer methyl methacrylate (MMA), N-dimethylaminoethyl methacrylate hydrochloride (DMAEMC), and N-trimethylaminoethyl methacrylate chloride (TMAEMC), which show a higher solubility than chitosan in a broader pH range, have been prepared by free radical polymerization. The nanoparticles were characterized in terms of particle size, zeta potential, TEM, and FT-IR. These nanoparticles were 150-280 nm in size and carried obvious positive surface charges. Protein-loaded nanoparticles were prepared, and their maximal encapsulation efficiency was up to 100%. In vitro release showed that these nanoparticles provided an initial burst release followed by a slowly sustained release for more than 24 h. These graft copolymer nanoparticles enhanced the absorption and improved the bioavailability of insulin via the gastrointestinal (GI) tract of normal male Sprague-Dawley (SD) strain rats to a greater extent than that of the phosphate buffer solution (PBS) of insulin.
Zhen Wang - One of the best experts on this subject based on the ideXlab platform.
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gradient cross linked biodegradable polyelectrolyte nanocapsules for intracellular Protein Drug Delivery
Biomaterials, 2010Co-Authors: Shujun Shu, Xinge Zhang, Zhen WangAbstract:Gradient shell cross-linked hollow polyelectrolyte nanocapsules composed of cysteamine conjugated chitosan and dextran sulfate were prepared by layer-by-layer adsorption on β-cyclodextrin (β-CD) functionalized silica spheres followed by cross-linking thiols and removal of silica core. This disulfide bond gradient cross-linked nanocapsules combined reduction and pH sensitive. Gradually increased from the inside to the outside of the cross-linking degree, one purpose is to ensure that cross-linking disulfide bond after reduction cleavage still has pH sensitive, on the other hand is to avoid cross-linked contraction of internal damage the crystal and bioactivity of Protein Drugs. Disulfide cross-linked nanocapsules were used to enhance the physical stability against acidic pH conditions compared to the un-cross-linked ones. Bovine serum albumin, as a model Protein Drug, was loaded inside nanocapsules. The disulfide bond cross-linked nanocapsules are intended to remain more stable in physiological pH and decrease the loss of Protein Drugs caused by the gastric cavity, and can release the Drugs in the intracellular environment after glutathione reduction.
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hollow and degradable polyelectrolyte nanocapsules for Protein Drug Delivery
Acta Biomaterialia, 2010Co-Authors: Shujun Shu, Xinge Zhang, Chunyang Sun, Zhen WangAbstract:Biodegradable hollow capsules encapsulating Protein Drugs were prepared via layer-by-layer assembly of water-soluble chitosan and dextran sulfate on Protein-entrapping amino-functionalized silica particles and the subsequent removal of the silica. In order to enhance the encapsulated efficiency and decrease its burst release, we designed this new system to fulfill these two goals. Bovine serum albumin (BSA), which was used as model Protein, was entrapped in the nanocapsules. This system demonstrated a good capacity for the encapsulation and loading of BSA. The burst release was decreased to less than 10% in phosphate-buffered saline within 2h. No significant conformation change was noted from the released BSA in comparison with native BSA by using circular dichroism spectroscopy. Cell viability study suggested that the nanocapsules had good biocompatibility. The Drug release kinetics mechanism is Fickian diffusion. These kinds of novel composite nanocapsules may offer a promising Delivery system for water-soluble Proteins and peptides.
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polyelectrolyte nanoparticles based on water soluble chitosan poly l aspartic acid polyethylene glycol for controlled Protein release
Carbohydrate Research, 2009Co-Authors: Shujun Shu, Xinge Zhang, Dayong Teng, Zhen WangAbstract:Water-soluble chitosan (WSC)-poly(L-aspartic acid) (PASP)-polyethylene glycol (PEG) nanoparticles (CPP nanoparticles) were prepared spontaneously under quite mild conditions by polyelectrolyte complexation. These nanoparticles were well dispersed and stable in aqueous solution, and their physicochemical properties were characterized by turbidity, FTIR spectroscopy, dynamic light scattering (DLS), transmission electron microscope (TEM), and zeta potential. PEG was chosen to modify WSC-PASP nanoparticles to make a Protein-protective agent. Investigation on the encapsulation efficiency and loading capacity of the bovine serum albumin (BSA)-loaded CPP nanoparticles was also conducted. Encapsulation efficiency was obviously decreased with the increase of initial BSA concentration. Furthermore, its in vitro release characteristics were evaluated at pH 1.2, 2.5, and 7.4. In vitro release showed that these nanoparticles provided an initial burst release, followed by a slowly sustained release for more than 24 h. The BSA released from CPP nanoparticles showed no significant conformational change compared with native BSA, which is superior to the BSA released from nanoparticles without PEG. A cell viability study suggested that the nanoparticles had good biocompatibility. This nanoparticle system was considered promising as an advanced Drug Delivery system for the peptide and Protein Drug Delivery.
Yingjie Zhu - One of the best experts on this subject based on the ideXlab platform.
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hydroxyapatite nanorod assembled hierarchical microflowers rapid synthesis via microwave hydrothermal transformation of cahpo4 and their application in Protein Drug Delivery
Ceramics International, 2017Co-Authors: Yingjie ZhuAbstract:Hydroxyapatite (HAP) nanostructured materials have attracted much attention due to their excellent biocompatibility and promising applications in various biomedical fields. In this study, a facile method has been developed to synthesize HAP with flower-like hierarchical nanostructures. The flower-like CaHPO4 precursor is firstly synthesized using triethyl phosphate (TEP) as the organic phosphorus source by the solvothermal method. The HAP hierarchical microflowers constructed with nanorods are then fabricated through rapid microwave hydrothermal transformation of the CaHPO4 precursor in NaOH aqueous solution. The as-prepared HAP nanorod-assembled hierarchical microflowers are explored to study the Protein/Drug loading and release properties using hemoglobin (Hb) and ibuprofen (IBU) as a model Protein and Drug, respectively. The experimental results indicate that the as-prepared HAP nanorod-assembled hierarchical microflowers have relatively large specific surface area, high biocompatibility, high Protein/Drug loading capacity and pH-dependent sustained release properties. Thus, the as-prepared HAP nanorod-assembled hierarchical microflowers are promising for the applications in Protein/Drug Delivery.
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sonochemical synthesis of hydroxyapatite nanoflowers using creatine phosphate disodium salt as an organic phosphorus source and their application in Protein adsorption
RSC Advances, 2016Co-Authors: Yingjie Zhu, Tuanwei Sun, Yingying Jiang, Yonggang Zhang, Feng ChenAbstract:In this paper, the one-step rapid synthesis of hydroxyapatite nanoflowers (HAFs) using creatine phosphate disodium salt as an organic phosphorus source by the sonochemical method is reported. The HAFs with diameters of about 300 nm are formed by self-assembly of hydroxyapatite nanosheets with thicknesses of less than 10 nm. The as-prepared samples are characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Brunauer–Emmett–Teller (BET) nitrogen sorptometry, X-ray powder diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy and inductively coupled plasma (ICP) optical emission spectroscopy. The MTT tests show that the as-prepared HAFs exhibit essentially inappreciable toxicity to MC-3T3 osteoblast cells, indicating that the HAFs have an excellent cytocompatibility. Moreover, the as-prepared HAFs show a relatively high Protein adsorption ability when using hemoglobin (Hb) as a model Protein. Thus, the as-prepared HAFs are promising for applications in various biomedical fields such as Protein/Drug Delivery.
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yolk shell porous microspheres of calcium phosphate prepared by using calcium l lactate and adenosine 5 triphosphate disodium salt application in Protein Drug Delivery
Chemistry: A European Journal, 2015Co-Authors: Guanjun Ding, Tuanwei Sun, Yingjie Zhu, Feng ChenAbstract:A facile and environmentally friendly approach has been developed to prepare yolk-shell porous microspheres of calcium phosphate by using calcium L-lactate pentahydrate (CL) as the calcium source and adenosine 5′-triphosphate disodium salt (ATP) as the phosphate source through the microwave-assisted hydrothermal method. The effects of the concentration of CL, the microwave hydrothermal temperature, and the time on the morphology and crystal phase of the product are investigated. The possible formation mechanism of yolk-shell porous microspheres of calcium phosphate is proposed. Hemoglobin from bovine red cells (Hb) and ibuprofen (IBU) are used to explore the application potential of yolk-shell porous microspheres of calcium phosphate in Protein/Drug loading and Delivery. The experimental results indicate that the as-prepared yolk-shell porous microspheres of calcium phosphate have relatively high Protein/Drug loading capacity, sustained Protein/Drug release, favorable pH-responsive release behavior, and a high biocompatibility in the cytotoxicity test. Therefore, the yolk-shell porous microspheres of calcium phosphate have promising applications in various biomedical fields such as Protein/Drug Delivery.
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nanosheet assembled hierarchical nanostructures of hydroxyapatite surfactant free microwave hydrothermal rapid synthesis Protein dna adsorption and ph controlled release
CrystEngComm, 2013Co-Authors: Xinyu Zhao, Yingjie Zhu, Feng ChenAbstract:In this paper, a surfactant-free rapid microwave-assisted hydrothermal synthesis of hydroxyapatite nanosheet-assembled flower-like hierarchical nanostructures (NFHNs) is reported. The effects of the experimental conditions on the morphology and crystal phase of the product are investigated. A possible formation mechanism of hydroxyapatite NFHNs is proposed. The morphology of the product can vary from flower-like to polyhedra by adjusting the microwave heating temperature. The Protein and DNA adsorption properties of the as-prepared hydroxyapatite NFHNs are studied. The loading capacities of the as-prepared hydroxyapatite NFHNs for bovine serum albumin (BSA), hemoglobin (Hb) and fish sperm DNA are determined to be 165, 164 and 112 mg g−1, respectively. The Protein release process is conducted at different pH values (pH 7.2, 5.5 and 4.8) in phosphate buffer saline (PBS), and the pH-controlled Protein release behavior has been found. Thus, the as-prepared hydroxyapatite NFHNs are promising for Protein Drug Delivery applications.
Feng Qian - One of the best experts on this subject based on the ideXlab platform.
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chitosan graft copolymer nanoparticles for oral Protein Drug Delivery preparation and characterization
Biomacromolecules, 2006Co-Authors: Feng Qian, Jieying Ding, Cui TangAbstract:Several novel functionalized graft copolymer nanoparticles consisting of chitosan (CS) and the monomer methyl methacrylate (MMA), N-dimethylaminoethyl methacrylate hydrochloride (DMAEMC), and N-trimethylaminoethyl methacrylate chloride (TMAEMC), which show a higher solubility than chitosan in a broader pH range, have been prepared by free radical polymerization. The nanoparticles were characterized in terms of particle size, zeta potential, TEM, and FT-IR. These nanoparticles were 150−280 nm in size and carried obvious positive surface charges. Protein-loaded nanoparticles were prepared, and their maximal encapsulation efficiency was up to 100%. In vitro release showed that these nanoparticles provided an initial burst release followed by a slowly sustained release for more than 24 h. These graft copolymer nanoparticles enhanced the absorption and improved the bioavailability of insulin via the gastrointestinal (GI) tract of normal male Sprague−Dawley (SD) strain rats to a greater extent than that of the ...
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chitosan graft copolymer nanoparticles for oral Protein Drug Delivery preparation and characterization
Biomacromolecules, 2006Co-Authors: Feng Qian, Jieying Ding, Cui TangAbstract:Several novel functionalized graft copolymer nanoparticles consisting of chitosan (CS) and the monomer methyl methacrylate (MMA), N-dimethylaminoethyl methacrylate hydrochloride (DMAEMC), and N-trimethylaminoethyl methacrylate chloride (TMAEMC), which show a higher solubility than chitosan in a broader pH range, have been prepared by free radical polymerization. The nanoparticles were characterized in terms of particle size, zeta potential, TEM, and FT-IR. These nanoparticles were 150−280 nm in size and carried obvious positive surface charges. Protein-loaded nanoparticles were prepared, and their maximal encapsulation efficiency was up to 100%. In vitro release showed that these nanoparticles provided an initial burst release followed by a slowly sustained release for more than 24 h. These graft copolymer nanoparticles enhanced the absorption and improved the bioavailability of insulin via the gastrointestinal (GI) tract of normal male Sprague−Dawley (SD) strain rats to a greater extent than that of the ...
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chitosan graft copolymer nanoparticles for oral Protein Drug Delivery preparation and characterization
Biomacromolecules, 2006Co-Authors: Feng Qian, Jieying Ding, Cui Tang, Fuying Cui, Chunhua YinAbstract:Several novel functionalized graft copolymer nanoparticles consisting of chitosan (CS) and the monomer methyl methacrylate (MMA), N-dimethylaminoethyl methacrylate hydrochloride (DMAEMC), and N-trimethylaminoethyl methacrylate chloride (TMAEMC), which show a higher solubility than chitosan in a broader pH range, have been prepared by free radical polymerization. The nanoparticles were characterized in terms of particle size, zeta potential, TEM, and FT-IR. These nanoparticles were 150-280 nm in size and carried obvious positive surface charges. Protein-loaded nanoparticles were prepared, and their maximal encapsulation efficiency was up to 100%. In vitro release showed that these nanoparticles provided an initial burst release followed by a slowly sustained release for more than 24 h. These graft copolymer nanoparticles enhanced the absorption and improved the bioavailability of insulin via the gastrointestinal (GI) tract of normal male Sprague-Dawley (SD) strain rats to a greater extent than that of the phosphate buffer solution (PBS) of insulin.
