The Experts below are selected from a list of 18 Experts worldwide ranked by ideXlab platform
Stanisław Winiarczyk - One of the best experts on this subject based on the ideXlab platform.
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Urinary proteome of dogs with kidney injury during babesiosis
BMC veterinary research, 2019Co-Authors: Dagmara Winiarczyk, Katarzyna Michalak, Mateusz Winiarczyk, L. Adaszek, Stanisław WiniarczykAbstract:Acute kidney injury is the most frequent complication of babesiosis in dogs and may provide a natural model for identifying early and specific markers of kidney injury in this species. There are limited data on urine proteomics in dogs, and none of the effect of babesiosis on the urine proteome. This study aimed to identify urinary Proteins of dogs with kidney injury during the natural course of babesiosis caused by Babesia canis, and to compare them with Proteins in a control group to reveal any potential biomarkers predicting renal injury before the presence of azotemia. Urine samples were collected from 10 dogs of various breeds and sex with naturally occurring babesiosis, and 10 healthy dogs. Pooled urine samples from both groups were separated by 2D (two-dimensional) electrophoresis, followed by Protein identification using MALDI-TOF (matrix-assisted laser desorption ionization time of flight) mass spectrometry. In total, 176 Proteins were identified in the urine samples from healthy dogs, and 403 Proteins were identified in the urine samples from dogs with babesiosis. Of the 176 Proteins, 146 were assigned exclusively to healthy dogs, and 373 of the 403 Proteins were assigned exclusively to dogs with babesiosis; 30 Proteins were common for both groups. Characteristic analysis of 373 Proteins found in dogs with babesiosis led to the isolation of 8 Proteins associated with 10 metabolic pathways involved in immune and inflammatory responses. It was hypothesized that epithelial-mesenchymal transition might play an important role in the mechanisms underlying pathological changes in renal tissue during babesiosis, as indicated by a causal relationship network built by combining 5 of the 10 selected metabolic pathways, and 4 of the 8 Proteins associated with these pathways; this network included cadherins, gonadotropin releasing hormone receptors, inflammatory responses mediated by chemokine and cytokine signalling pathways, integrins, interleukins, and TGF-β (transforming growth factor β) pathways. Those pathways were linked by interleukin-13, bone morphogenetic Protein 7, α2(1) collagen, and tyrosine Protein Kinase Fer, which are potential biomarkers of damage during babesiosis in dogs, that might indicate early renal injury.
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Urinary proteome of dogs with kidney injury during babesiosis
BMC Veterinary Research, 2019Co-Authors: Dagmara Winiarczyk, Katarzyna Michalak, Mateusz Winiarczyk, L. Adaszek, Stanisław WiniarczykAbstract:Background Acute kidney injury is the most frequent complication of babesiosis in dogs and may provide a natural model for identifying early and specific markers of kidney injury in this species. There are limited data on urine proteomics in dogs, and none of the effect of babesiosis on the urine proteome. This study aimed to identify urinary Proteins of dogs with kidney injury during the natural course of babesiosis caused by Babesia canis, and to compare them with Proteins in a control group to reveal any potential biomarkers predicting renal injury before the presence of azotemia. Urine samples were collected from 10 dogs of various breeds and sex with naturally occurring babesiosis, and 10 healthy dogs. Pooled urine samples from both groups were separated by 2D (two-dimensional) electrophoresis, followed by Protein identification using MALDI-TOF (matrix-assisted laser desorption ionization time of flight) mass spectrometry. Results In total, 176 Proteins were identified in the urine samples from healthy dogs, and 403 Proteins were identified in the urine samples from dogs with babesiosis. Of the 176 Proteins, 146 were assigned exclusively to healthy dogs, and 373 of the 403 Proteins were assigned exclusively to dogs with babesiosis; 30 Proteins were common for both groups. Characteristic analysis of 373 Proteins found in dogs with babesiosis led to the isolation of 8 Proteins associated with 10 metabolic pathways involved in immune and inflammatory responses. Conclusions It was hypothesized that epithelial-mesenchymal transition might play an important role in the mechanisms underlying pathological changes in renal tissue during babesiosis, as indicated by a causal relationship network built by combining 5 of the 10 selected metabolic pathways, and 4 of the 8 Proteins associated with these pathways; this network included cadherins, gonadotropin releasing hormone receptors, inflammatory responses mediated by chemokine and cytokine signalling pathways, integrins, interleukins, and TGF-β (transforming growth factor β) pathways. Those pathways were linked by interleukin-13, bone morphogenetic Protein 7, α2(1) collagen, and tyrosine Protein Kinase Fer, which are potential biomarkers of damage during babesiosis in dogs, that might indicate early renal injury.
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Urinary proteome of dogs with kidney injury during babesiosis
2019Co-Authors: Dagmara Winiarczyk, Katarzyna Michalak, Łukasz Adaszek, Mateusz Winiarczyk, Stanisław WiniarczykAbstract:Abstract Background Acute kidney injury is the most frequent complication of babesiosis in dogs and may provide a natural model for identifying early and specific markers of kidney injury in this species. There are limited data on urine proteomics in dogs, and none of the effect of babesiosis on the urine proteome. This study aimed to identify urinary Proteins of dogs with kidney injury during the natural course of babesiosis caused by Babesia canis, and to compare them with Proteins in a control group to reveal any potential biomarkers predicting renal injury before the presence of azotemia. Urine samples were collected from 10 dogs of various breeds and sex with naturally occurring babesiosis, and 10 healthy dogs. Pooled urine samples from both groups were separated by 2D (two-dimensional) electrophoresis, followed by Protein identification using MALDI-TOF (matrix-assisted laser desorption ionization time of flight) mass spectrometry. Results In total, 176 Proteins were identified in the urine samples from healthy dogs, and 403 Proteins were identified in the urine samples from dogs with babesiosis. Of the 176 Proteins, 146 were assigned exclusively to healthy dogs, and 373 of the 403 Proteins were assigned exclusively to dogs with babesiosis; 30 Proteins were common for both groups. Characteristic analysis of 373 Proteins found in dogs with babesiosis led to the isolation of 8 Proteins associated with 10 metabolic pathways involved in immune and inflammatory responses. Conclusions It was hypothesized that epithelial-mesenchymal transition might play an important role in the mechanisms underlying pathological changes in renal tissue during babesiosis, as indicated by a causal relationship network built by combining 5 of the 10 selected metabolic pathways, and 4 of the 8 Proteins associated with these pathways; this network included cadherins, gonadotropin releasing hormone receptors, inflammatory responses mediated by chemokine and cytokine signalling pathways, integrins, interleukins, and TGF-β (transforming growth factor β) pathways. Those pathways were linked by interleukin-13, bone morphogenetic Protein 7, α2(1) collagen, and tyrosine Protein Kinase Fer, which are potential biomarkers of damage during babesiosis in dogs, that might indicate early renal injury. Keywords: Acute kidney injury, Babesiosis, Dog, Proteomics, Urine
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Dog Tear Film Proteome In-Depth Analysis
PloS one, 2015Co-Authors: Mateusz Winiarczyk, Dagmara Winiarczyk, Tomasz Banach, L. Adaszek, Jacek Madany, Jerzy Mackiewicz, Dorota Pietras-ożga, Stanisław WiniarczykAbstract:In this study, mass spectrometry was used to explore the canine tear proteome. Tear samples were obtained from six healthy dogs, and one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (1D SDS-PAGE) was used as a first step to separate intact Proteins into 17 bands. Each fraction was then trypsin digested and analysed by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF-MS/MS) to characterize the Protein components in each fraction. In total, 125 tear Proteins were identified, with MCA (Major Canine Allergen), Serum albumin, UPF0557 Protein C10orf119 homolog, Collagen alpha-2(I) chain, Tyrosine -Protein Kinase Fer, Keratine type II cytoskeletal, Beta-crystallin B2, Interleukin-6 and Desmin occuring as the most confident ones with the highest scores. The results showed that the proteomic strategy used in this study was successful in the analysis of the dog tear proteome. To the best of our knowledge, this study is the first to report the comprehensive proteome profile of tears from healthy dogs by 1D SDS PAGE and MALDI-TOF. Data are available via ProteomeXchange with identifier PXD003124.
Mateusz Winiarczyk - One of the best experts on this subject based on the ideXlab platform.
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Urinary proteome of dogs with kidney injury during babesiosis
BMC veterinary research, 2019Co-Authors: Dagmara Winiarczyk, Katarzyna Michalak, Mateusz Winiarczyk, L. Adaszek, Stanisław WiniarczykAbstract:Acute kidney injury is the most frequent complication of babesiosis in dogs and may provide a natural model for identifying early and specific markers of kidney injury in this species. There are limited data on urine proteomics in dogs, and none of the effect of babesiosis on the urine proteome. This study aimed to identify urinary Proteins of dogs with kidney injury during the natural course of babesiosis caused by Babesia canis, and to compare them with Proteins in a control group to reveal any potential biomarkers predicting renal injury before the presence of azotemia. Urine samples were collected from 10 dogs of various breeds and sex with naturally occurring babesiosis, and 10 healthy dogs. Pooled urine samples from both groups were separated by 2D (two-dimensional) electrophoresis, followed by Protein identification using MALDI-TOF (matrix-assisted laser desorption ionization time of flight) mass spectrometry. In total, 176 Proteins were identified in the urine samples from healthy dogs, and 403 Proteins were identified in the urine samples from dogs with babesiosis. Of the 176 Proteins, 146 were assigned exclusively to healthy dogs, and 373 of the 403 Proteins were assigned exclusively to dogs with babesiosis; 30 Proteins were common for both groups. Characteristic analysis of 373 Proteins found in dogs with babesiosis led to the isolation of 8 Proteins associated with 10 metabolic pathways involved in immune and inflammatory responses. It was hypothesized that epithelial-mesenchymal transition might play an important role in the mechanisms underlying pathological changes in renal tissue during babesiosis, as indicated by a causal relationship network built by combining 5 of the 10 selected metabolic pathways, and 4 of the 8 Proteins associated with these pathways; this network included cadherins, gonadotropin releasing hormone receptors, inflammatory responses mediated by chemokine and cytokine signalling pathways, integrins, interleukins, and TGF-β (transforming growth factor β) pathways. Those pathways were linked by interleukin-13, bone morphogenetic Protein 7, α2(1) collagen, and tyrosine Protein Kinase Fer, which are potential biomarkers of damage during babesiosis in dogs, that might indicate early renal injury.
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Urinary proteome of dogs with kidney injury during babesiosis
BMC Veterinary Research, 2019Co-Authors: Dagmara Winiarczyk, Katarzyna Michalak, Mateusz Winiarczyk, L. Adaszek, Stanisław WiniarczykAbstract:Background Acute kidney injury is the most frequent complication of babesiosis in dogs and may provide a natural model for identifying early and specific markers of kidney injury in this species. There are limited data on urine proteomics in dogs, and none of the effect of babesiosis on the urine proteome. This study aimed to identify urinary Proteins of dogs with kidney injury during the natural course of babesiosis caused by Babesia canis, and to compare them with Proteins in a control group to reveal any potential biomarkers predicting renal injury before the presence of azotemia. Urine samples were collected from 10 dogs of various breeds and sex with naturally occurring babesiosis, and 10 healthy dogs. Pooled urine samples from both groups were separated by 2D (two-dimensional) electrophoresis, followed by Protein identification using MALDI-TOF (matrix-assisted laser desorption ionization time of flight) mass spectrometry. Results In total, 176 Proteins were identified in the urine samples from healthy dogs, and 403 Proteins were identified in the urine samples from dogs with babesiosis. Of the 176 Proteins, 146 were assigned exclusively to healthy dogs, and 373 of the 403 Proteins were assigned exclusively to dogs with babesiosis; 30 Proteins were common for both groups. Characteristic analysis of 373 Proteins found in dogs with babesiosis led to the isolation of 8 Proteins associated with 10 metabolic pathways involved in immune and inflammatory responses. Conclusions It was hypothesized that epithelial-mesenchymal transition might play an important role in the mechanisms underlying pathological changes in renal tissue during babesiosis, as indicated by a causal relationship network built by combining 5 of the 10 selected metabolic pathways, and 4 of the 8 Proteins associated with these pathways; this network included cadherins, gonadotropin releasing hormone receptors, inflammatory responses mediated by chemokine and cytokine signalling pathways, integrins, interleukins, and TGF-β (transforming growth factor β) pathways. Those pathways were linked by interleukin-13, bone morphogenetic Protein 7, α2(1) collagen, and tyrosine Protein Kinase Fer, which are potential biomarkers of damage during babesiosis in dogs, that might indicate early renal injury.
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Urinary proteome of dogs with kidney injury during babesiosis
2019Co-Authors: Dagmara Winiarczyk, Katarzyna Michalak, Łukasz Adaszek, Mateusz Winiarczyk, Stanisław WiniarczykAbstract:Abstract Background Acute kidney injury is the most frequent complication of babesiosis in dogs and may provide a natural model for identifying early and specific markers of kidney injury in this species. There are limited data on urine proteomics in dogs, and none of the effect of babesiosis on the urine proteome. This study aimed to identify urinary Proteins of dogs with kidney injury during the natural course of babesiosis caused by Babesia canis, and to compare them with Proteins in a control group to reveal any potential biomarkers predicting renal injury before the presence of azotemia. Urine samples were collected from 10 dogs of various breeds and sex with naturally occurring babesiosis, and 10 healthy dogs. Pooled urine samples from both groups were separated by 2D (two-dimensional) electrophoresis, followed by Protein identification using MALDI-TOF (matrix-assisted laser desorption ionization time of flight) mass spectrometry. Results In total, 176 Proteins were identified in the urine samples from healthy dogs, and 403 Proteins were identified in the urine samples from dogs with babesiosis. Of the 176 Proteins, 146 were assigned exclusively to healthy dogs, and 373 of the 403 Proteins were assigned exclusively to dogs with babesiosis; 30 Proteins were common for both groups. Characteristic analysis of 373 Proteins found in dogs with babesiosis led to the isolation of 8 Proteins associated with 10 metabolic pathways involved in immune and inflammatory responses. Conclusions It was hypothesized that epithelial-mesenchymal transition might play an important role in the mechanisms underlying pathological changes in renal tissue during babesiosis, as indicated by a causal relationship network built by combining 5 of the 10 selected metabolic pathways, and 4 of the 8 Proteins associated with these pathways; this network included cadherins, gonadotropin releasing hormone receptors, inflammatory responses mediated by chemokine and cytokine signalling pathways, integrins, interleukins, and TGF-β (transforming growth factor β) pathways. Those pathways were linked by interleukin-13, bone morphogenetic Protein 7, α2(1) collagen, and tyrosine Protein Kinase Fer, which are potential biomarkers of damage during babesiosis in dogs, that might indicate early renal injury. Keywords: Acute kidney injury, Babesiosis, Dog, Proteomics, Urine
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Dog Tear Film Proteome In-Depth Analysis
PloS one, 2015Co-Authors: Mateusz Winiarczyk, Dagmara Winiarczyk, Tomasz Banach, L. Adaszek, Jacek Madany, Jerzy Mackiewicz, Dorota Pietras-ożga, Stanisław WiniarczykAbstract:In this study, mass spectrometry was used to explore the canine tear proteome. Tear samples were obtained from six healthy dogs, and one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (1D SDS-PAGE) was used as a first step to separate intact Proteins into 17 bands. Each fraction was then trypsin digested and analysed by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF-MS/MS) to characterize the Protein components in each fraction. In total, 125 tear Proteins were identified, with MCA (Major Canine Allergen), Serum albumin, UPF0557 Protein C10orf119 homolog, Collagen alpha-2(I) chain, Tyrosine -Protein Kinase Fer, Keratine type II cytoskeletal, Beta-crystallin B2, Interleukin-6 and Desmin occuring as the most confident ones with the highest scores. The results showed that the proteomic strategy used in this study was successful in the analysis of the dog tear proteome. To the best of our knowledge, this study is the first to report the comprehensive proteome profile of tears from healthy dogs by 1D SDS PAGE and MALDI-TOF. Data are available via ProteomeXchange with identifier PXD003124.
Tomasz Banach - One of the best experts on this subject based on the ideXlab platform.
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RESEARCH ARTICLE Dog Tear Film Proteome In-Depth Analysis
2016Co-Authors: Tomasz Banach, L. Adaszek, Jacek Madany, Jerzy Mackiewicz, Dorota Pietras-ozgaAbstract:In this study, mass spectrometry was used to explore the canine tear proteome. Tear sam-ples were obtained from six healthy dogs, and one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (1D SDS-PAGE) was used as a first step to separate intact Proteins into 17 bands. Each fraction was then trypsin digested and analysed by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF-MS/MS) to characterize the Protein components in each fraction. In total, 125 tear Proteins were identified, with MCA (Major Canine Allergen), Serum albumin, UPF0557 Protein C10orf119 homolog, Collagen alpha-2(I) chain, Tyrosine-Protein Kinase Fer, Kera-tine type II cytoskeletal, Beta-crystallin B2, Interleukin-6 and Desmin occuring as the most confident ones with the highest scores. The results showed that the proteomic strategy used in this study was successful in the analysis of the dog tear proteome. To the best of our knowledge, this study is the first to report the comprehensive proteome profile of tears from healthy dogs by 1D SDS PAGE and MALDI-TOF. Data are available via ProteomeX-change with identifier PXD003124
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Dog Tear Film Proteome In-Depth Analysis
PloS one, 2015Co-Authors: Mateusz Winiarczyk, Dagmara Winiarczyk, Tomasz Banach, L. Adaszek, Jacek Madany, Jerzy Mackiewicz, Dorota Pietras-ożga, Stanisław WiniarczykAbstract:In this study, mass spectrometry was used to explore the canine tear proteome. Tear samples were obtained from six healthy dogs, and one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (1D SDS-PAGE) was used as a first step to separate intact Proteins into 17 bands. Each fraction was then trypsin digested and analysed by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF-MS/MS) to characterize the Protein components in each fraction. In total, 125 tear Proteins were identified, with MCA (Major Canine Allergen), Serum albumin, UPF0557 Protein C10orf119 homolog, Collagen alpha-2(I) chain, Tyrosine -Protein Kinase Fer, Keratine type II cytoskeletal, Beta-crystallin B2, Interleukin-6 and Desmin occuring as the most confident ones with the highest scores. The results showed that the proteomic strategy used in this study was successful in the analysis of the dog tear proteome. To the best of our knowledge, this study is the first to report the comprehensive proteome profile of tears from healthy dogs by 1D SDS PAGE and MALDI-TOF. Data are available via ProteomeXchange with identifier PXD003124.
Dagmara Winiarczyk - One of the best experts on this subject based on the ideXlab platform.
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Urinary proteome of dogs with kidney injury during babesiosis
BMC veterinary research, 2019Co-Authors: Dagmara Winiarczyk, Katarzyna Michalak, Mateusz Winiarczyk, L. Adaszek, Stanisław WiniarczykAbstract:Acute kidney injury is the most frequent complication of babesiosis in dogs and may provide a natural model for identifying early and specific markers of kidney injury in this species. There are limited data on urine proteomics in dogs, and none of the effect of babesiosis on the urine proteome. This study aimed to identify urinary Proteins of dogs with kidney injury during the natural course of babesiosis caused by Babesia canis, and to compare them with Proteins in a control group to reveal any potential biomarkers predicting renal injury before the presence of azotemia. Urine samples were collected from 10 dogs of various breeds and sex with naturally occurring babesiosis, and 10 healthy dogs. Pooled urine samples from both groups were separated by 2D (two-dimensional) electrophoresis, followed by Protein identification using MALDI-TOF (matrix-assisted laser desorption ionization time of flight) mass spectrometry. In total, 176 Proteins were identified in the urine samples from healthy dogs, and 403 Proteins were identified in the urine samples from dogs with babesiosis. Of the 176 Proteins, 146 were assigned exclusively to healthy dogs, and 373 of the 403 Proteins were assigned exclusively to dogs with babesiosis; 30 Proteins were common for both groups. Characteristic analysis of 373 Proteins found in dogs with babesiosis led to the isolation of 8 Proteins associated with 10 metabolic pathways involved in immune and inflammatory responses. It was hypothesized that epithelial-mesenchymal transition might play an important role in the mechanisms underlying pathological changes in renal tissue during babesiosis, as indicated by a causal relationship network built by combining 5 of the 10 selected metabolic pathways, and 4 of the 8 Proteins associated with these pathways; this network included cadherins, gonadotropin releasing hormone receptors, inflammatory responses mediated by chemokine and cytokine signalling pathways, integrins, interleukins, and TGF-β (transforming growth factor β) pathways. Those pathways were linked by interleukin-13, bone morphogenetic Protein 7, α2(1) collagen, and tyrosine Protein Kinase Fer, which are potential biomarkers of damage during babesiosis in dogs, that might indicate early renal injury.
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Urinary proteome of dogs with kidney injury during babesiosis
BMC Veterinary Research, 2019Co-Authors: Dagmara Winiarczyk, Katarzyna Michalak, Mateusz Winiarczyk, L. Adaszek, Stanisław WiniarczykAbstract:Background Acute kidney injury is the most frequent complication of babesiosis in dogs and may provide a natural model for identifying early and specific markers of kidney injury in this species. There are limited data on urine proteomics in dogs, and none of the effect of babesiosis on the urine proteome. This study aimed to identify urinary Proteins of dogs with kidney injury during the natural course of babesiosis caused by Babesia canis, and to compare them with Proteins in a control group to reveal any potential biomarkers predicting renal injury before the presence of azotemia. Urine samples were collected from 10 dogs of various breeds and sex with naturally occurring babesiosis, and 10 healthy dogs. Pooled urine samples from both groups were separated by 2D (two-dimensional) electrophoresis, followed by Protein identification using MALDI-TOF (matrix-assisted laser desorption ionization time of flight) mass spectrometry. Results In total, 176 Proteins were identified in the urine samples from healthy dogs, and 403 Proteins were identified in the urine samples from dogs with babesiosis. Of the 176 Proteins, 146 were assigned exclusively to healthy dogs, and 373 of the 403 Proteins were assigned exclusively to dogs with babesiosis; 30 Proteins were common for both groups. Characteristic analysis of 373 Proteins found in dogs with babesiosis led to the isolation of 8 Proteins associated with 10 metabolic pathways involved in immune and inflammatory responses. Conclusions It was hypothesized that epithelial-mesenchymal transition might play an important role in the mechanisms underlying pathological changes in renal tissue during babesiosis, as indicated by a causal relationship network built by combining 5 of the 10 selected metabolic pathways, and 4 of the 8 Proteins associated with these pathways; this network included cadherins, gonadotropin releasing hormone receptors, inflammatory responses mediated by chemokine and cytokine signalling pathways, integrins, interleukins, and TGF-β (transforming growth factor β) pathways. Those pathways were linked by interleukin-13, bone morphogenetic Protein 7, α2(1) collagen, and tyrosine Protein Kinase Fer, which are potential biomarkers of damage during babesiosis in dogs, that might indicate early renal injury.
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Urinary proteome of dogs with kidney injury during babesiosis
2019Co-Authors: Dagmara Winiarczyk, Katarzyna Michalak, Łukasz Adaszek, Mateusz Winiarczyk, Stanisław WiniarczykAbstract:Abstract Background Acute kidney injury is the most frequent complication of babesiosis in dogs and may provide a natural model for identifying early and specific markers of kidney injury in this species. There are limited data on urine proteomics in dogs, and none of the effect of babesiosis on the urine proteome. This study aimed to identify urinary Proteins of dogs with kidney injury during the natural course of babesiosis caused by Babesia canis, and to compare them with Proteins in a control group to reveal any potential biomarkers predicting renal injury before the presence of azotemia. Urine samples were collected from 10 dogs of various breeds and sex with naturally occurring babesiosis, and 10 healthy dogs. Pooled urine samples from both groups were separated by 2D (two-dimensional) electrophoresis, followed by Protein identification using MALDI-TOF (matrix-assisted laser desorption ionization time of flight) mass spectrometry. Results In total, 176 Proteins were identified in the urine samples from healthy dogs, and 403 Proteins were identified in the urine samples from dogs with babesiosis. Of the 176 Proteins, 146 were assigned exclusively to healthy dogs, and 373 of the 403 Proteins were assigned exclusively to dogs with babesiosis; 30 Proteins were common for both groups. Characteristic analysis of 373 Proteins found in dogs with babesiosis led to the isolation of 8 Proteins associated with 10 metabolic pathways involved in immune and inflammatory responses. Conclusions It was hypothesized that epithelial-mesenchymal transition might play an important role in the mechanisms underlying pathological changes in renal tissue during babesiosis, as indicated by a causal relationship network built by combining 5 of the 10 selected metabolic pathways, and 4 of the 8 Proteins associated with these pathways; this network included cadherins, gonadotropin releasing hormone receptors, inflammatory responses mediated by chemokine and cytokine signalling pathways, integrins, interleukins, and TGF-β (transforming growth factor β) pathways. Those pathways were linked by interleukin-13, bone morphogenetic Protein 7, α2(1) collagen, and tyrosine Protein Kinase Fer, which are potential biomarkers of damage during babesiosis in dogs, that might indicate early renal injury. Keywords: Acute kidney injury, Babesiosis, Dog, Proteomics, Urine
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Dog Tear Film Proteome In-Depth Analysis
PloS one, 2015Co-Authors: Mateusz Winiarczyk, Dagmara Winiarczyk, Tomasz Banach, L. Adaszek, Jacek Madany, Jerzy Mackiewicz, Dorota Pietras-ożga, Stanisław WiniarczykAbstract:In this study, mass spectrometry was used to explore the canine tear proteome. Tear samples were obtained from six healthy dogs, and one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (1D SDS-PAGE) was used as a first step to separate intact Proteins into 17 bands. Each fraction was then trypsin digested and analysed by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF-MS/MS) to characterize the Protein components in each fraction. In total, 125 tear Proteins were identified, with MCA (Major Canine Allergen), Serum albumin, UPF0557 Protein C10orf119 homolog, Collagen alpha-2(I) chain, Tyrosine -Protein Kinase Fer, Keratine type II cytoskeletal, Beta-crystallin B2, Interleukin-6 and Desmin occuring as the most confident ones with the highest scores. The results showed that the proteomic strategy used in this study was successful in the analysis of the dog tear proteome. To the best of our knowledge, this study is the first to report the comprehensive proteome profile of tears from healthy dogs by 1D SDS PAGE and MALDI-TOF. Data are available via ProteomeXchange with identifier PXD003124.
L. Adaszek - One of the best experts on this subject based on the ideXlab platform.
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RESEARCH ARTICLE Dog Tear Film Proteome In-Depth Analysis
2016Co-Authors: Tomasz Banach, L. Adaszek, Jacek Madany, Jerzy Mackiewicz, Dorota Pietras-ozgaAbstract:In this study, mass spectrometry was used to explore the canine tear proteome. Tear sam-ples were obtained from six healthy dogs, and one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (1D SDS-PAGE) was used as a first step to separate intact Proteins into 17 bands. Each fraction was then trypsin digested and analysed by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF-MS/MS) to characterize the Protein components in each fraction. In total, 125 tear Proteins were identified, with MCA (Major Canine Allergen), Serum albumin, UPF0557 Protein C10orf119 homolog, Collagen alpha-2(I) chain, Tyrosine-Protein Kinase Fer, Kera-tine type II cytoskeletal, Beta-crystallin B2, Interleukin-6 and Desmin occuring as the most confident ones with the highest scores. The results showed that the proteomic strategy used in this study was successful in the analysis of the dog tear proteome. To the best of our knowledge, this study is the first to report the comprehensive proteome profile of tears from healthy dogs by 1D SDS PAGE and MALDI-TOF. Data are available via ProteomeX-change with identifier PXD003124
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Dog Tear Film Proteome In-Depth Analysis
PloS one, 2015Co-Authors: Mateusz Winiarczyk, Dagmara Winiarczyk, Tomasz Banach, L. Adaszek, Jacek Madany, Jerzy Mackiewicz, Dorota Pietras-ożga, Stanisław WiniarczykAbstract:In this study, mass spectrometry was used to explore the canine tear proteome. Tear samples were obtained from six healthy dogs, and one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (1D SDS-PAGE) was used as a first step to separate intact Proteins into 17 bands. Each fraction was then trypsin digested and analysed by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF-MS/MS) to characterize the Protein components in each fraction. In total, 125 tear Proteins were identified, with MCA (Major Canine Allergen), Serum albumin, UPF0557 Protein C10orf119 homolog, Collagen alpha-2(I) chain, Tyrosine -Protein Kinase Fer, Keratine type II cytoskeletal, Beta-crystallin B2, Interleukin-6 and Desmin occuring as the most confident ones with the highest scores. The results showed that the proteomic strategy used in this study was successful in the analysis of the dog tear proteome. To the best of our knowledge, this study is the first to report the comprehensive proteome profile of tears from healthy dogs by 1D SDS PAGE and MALDI-TOF. Data are available via ProteomeXchange with identifier PXD003124.
