The Experts below are selected from a list of 117 Experts worldwide ranked by ideXlab platform
Mallika Imwong - One of the best experts on this subject based on the ideXlab platform.
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Genetic diversity of three surface Protein genes in Plasmodium malariae from three Asian countries
Malaria Journal, 2018Co-Authors: Suttipat Srisutham, Naowarat Saralamba, Kanlaya Sriprawat, Mayfong Mayxay, Frank Smithuis, Francois Nosten, Sasithon Pukrittayakamee, Arjen M. Dondorp, Mallika ImwongAbstract:Background Genetic diversity of the three important antigenic Proteins, namely thrombospondin-related anonymous Protein (TRAP), apical membrane antigen 1 (AMA1), and 6-cysteine Protein (P48/45), all of which are found in various developmental stages of Plasmodium parasites is crucial for targeted vaccine development. While studies related to the genetic diversity of these Proteins are available for Plasmodium falciparum and Plasmodium vivax , barely enough information exists regarding Plasmodium malariae . The present study aims to demonstrate the genetic variations existing among these three genes in P. malariae by analysing their diversity at nucleotide and Protein levels. Methods Three surface Protein genes were isolated from 45 samples collected in Thailand (N = 33), Myanmar (N = 8), and Lao PDR (N = 4), using conventional polymerase chain reaction (PCR) assay. Then, the PCR products were sequenced and analysed using BioEdit, MEGA6, and DnaSP programs. Results The average pairwise nucleotide diversities (π) of P. malariae trap , ama1 , and P48/45 were 0.00169, 0.00413, and 0.00029, respectively. The haplotype diversities (Hd) of P. malariae trap , ama1 , and P48/45 were 0.919, 0.946, and 0.130, respectively. Most of the nucleotide substitutions were non-synonymous, which indicated that the genetic variations of these genes were maintained by positive diversifying selection, thus, suggesting their role as a potential target of protective immune response. Amino acid substitutions of P. malariae TRAP, AMA1, and P48/45 could be categorized to 17, 20, and 2 unique amino-acid variants, respectively. For further vaccine development, carboxyl terminal of P48/45 would be a good candidate according to conserved amino acid at low genetic diversity (π = 0.2–0.3). Conclusions High mutational diversity was observed in P. malariae trap and ama1 as compared to P48/45 in P. malariae samples isolated from Thailand, Myanmar, and Lao PDR. Taken together, these results suggest that P48/45 might be a good vaccine candidate against P. malariae infection because of its sufficiently low genetic diversity and highly conserved amino acids especially on the carboxyl end.
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Genetic diversity of three surface Protein genes in Plasmodium malariae from three Asian countries.
Malaria Journal, 2018Co-Authors: Suttipat Srisutham, Naowarat Saralamba, Kanlaya Sriprawat, Mayfong Mayxay, Frank Smithuis, Francois Nosten, Sasithon Pukrittayakamee, Arjen M. Dondorp, Mallika ImwongAbstract:Genetic diversity of the three important antigenic Proteins, namely thrombospondin-related anonymous Protein (TRAP), apical membrane antigen 1 (AMA1), and 6-cysteine Protein (P48/45), all of which are found in various developmental stages of Plasmodium parasites is crucial for targeted vaccine development. While studies related to the genetic diversity of these Proteins are available for Plasmodium falciparum and Plasmodium vivax, barely enough information exists regarding Plasmodium malariae. The present study aims to demonstrate the genetic variations existing among these three genes in P. malariae by analysing their diversity at nucleotide and Protein levels. Three surface Protein genes were isolated from 45 samples collected in Thailand (N = 33), Myanmar (N = 8), and Lao PDR (N = 4), using conventional polymerase chain reaction (PCR) assay. Then, the PCR products were sequenced and analysed using BioEdit, MEGA6, and DnaSP programs. The average pairwise nucleotide diversities (π) of P. malariae trap, ama1, and P48/45 were 0.00169, 0.00413, and 0.00029, respectively. The haplotype diversities (Hd) of P. malariae trap, ama1, and P48/45 were 0.919, 0.946, and 0.130, respectively. Most of the nucleotide substitutions were non-synonymous, which indicated that the genetic variations of these genes were maintained by positive diversifying selection, thus, suggesting their role as a potential target of protective immune response. Amino acid substitutions of P. malariae TRAP, AMA1, and P48/45 could be categorized to 17, 20, and 2 unique amino-acid variants, respectively. For further vaccine development, carboxyl terminal of P48/45 would be a good candidate according to conserved amino acid at low genetic diversity (π = 0.2–0.3). High mutational diversity was observed in P. malariae trap and ama1 as compared to P48/45 in P. malariae samples isolated from Thailand, Myanmar, and Lao PDR. Taken together, these results suggest that P48/45 might be a good vaccine candidate against P. malariae infection because of its sufficiently low genetic diversity and highly conserved amino acids especially on the carboxyl end.
Sung Hee Um - One of the best experts on this subject based on the ideXlab platform.
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long isoform of erbb3 binding Protein P48 mediates Protein kinase b akt dependent hdm2 stabilization and nuclear localization
Experimental Cell Research, 2012Co-Authors: Truong L X Nguyen, Sung Hee UmAbstract:P48 is a long isoform of the ErbB3 binding Protein that has oncogenic functions including promotion of carcinogenesis and induction of malignant transformation through negative regulation of tumor suppressor p53. Here, we show that high level of P48 Protein expression leads to enhance HDM2 phosphorylation by Akt and inhibits the self-ubiquitination of HDM2 by up-regulation of Akt activity, thereby promoting its Protein stability. Moreover, P48 expression leads to accumulated nuclear localization of HDM2, whereas P48 depletion disturbs its nuclear localization. Hence, higher expression of P48 in cancer cells reduces p53 levels through modulation of HDM2 nuclear localization and Protein stability via regulation of its Akt-mediated phosphorylation.
Suttipat Srisutham - One of the best experts on this subject based on the ideXlab platform.
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Genetic diversity of three surface Protein genes in Plasmodium malariae from three Asian countries
Malaria Journal, 2018Co-Authors: Suttipat Srisutham, Naowarat Saralamba, Kanlaya Sriprawat, Mayfong Mayxay, Frank Smithuis, Francois Nosten, Sasithon Pukrittayakamee, Arjen M. Dondorp, Mallika ImwongAbstract:Background Genetic diversity of the three important antigenic Proteins, namely thrombospondin-related anonymous Protein (TRAP), apical membrane antigen 1 (AMA1), and 6-cysteine Protein (P48/45), all of which are found in various developmental stages of Plasmodium parasites is crucial for targeted vaccine development. While studies related to the genetic diversity of these Proteins are available for Plasmodium falciparum and Plasmodium vivax , barely enough information exists regarding Plasmodium malariae . The present study aims to demonstrate the genetic variations existing among these three genes in P. malariae by analysing their diversity at nucleotide and Protein levels. Methods Three surface Protein genes were isolated from 45 samples collected in Thailand (N = 33), Myanmar (N = 8), and Lao PDR (N = 4), using conventional polymerase chain reaction (PCR) assay. Then, the PCR products were sequenced and analysed using BioEdit, MEGA6, and DnaSP programs. Results The average pairwise nucleotide diversities (π) of P. malariae trap , ama1 , and P48/45 were 0.00169, 0.00413, and 0.00029, respectively. The haplotype diversities (Hd) of P. malariae trap , ama1 , and P48/45 were 0.919, 0.946, and 0.130, respectively. Most of the nucleotide substitutions were non-synonymous, which indicated that the genetic variations of these genes were maintained by positive diversifying selection, thus, suggesting their role as a potential target of protective immune response. Amino acid substitutions of P. malariae TRAP, AMA1, and P48/45 could be categorized to 17, 20, and 2 unique amino-acid variants, respectively. For further vaccine development, carboxyl terminal of P48/45 would be a good candidate according to conserved amino acid at low genetic diversity (π = 0.2–0.3). Conclusions High mutational diversity was observed in P. malariae trap and ama1 as compared to P48/45 in P. malariae samples isolated from Thailand, Myanmar, and Lao PDR. Taken together, these results suggest that P48/45 might be a good vaccine candidate against P. malariae infection because of its sufficiently low genetic diversity and highly conserved amino acids especially on the carboxyl end.
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Genetic diversity of three surface Protein genes in Plasmodium malariae from three Asian countries.
Malaria Journal, 2018Co-Authors: Suttipat Srisutham, Naowarat Saralamba, Kanlaya Sriprawat, Mayfong Mayxay, Frank Smithuis, Francois Nosten, Sasithon Pukrittayakamee, Arjen M. Dondorp, Mallika ImwongAbstract:Genetic diversity of the three important antigenic Proteins, namely thrombospondin-related anonymous Protein (TRAP), apical membrane antigen 1 (AMA1), and 6-cysteine Protein (P48/45), all of which are found in various developmental stages of Plasmodium parasites is crucial for targeted vaccine development. While studies related to the genetic diversity of these Proteins are available for Plasmodium falciparum and Plasmodium vivax, barely enough information exists regarding Plasmodium malariae. The present study aims to demonstrate the genetic variations existing among these three genes in P. malariae by analysing their diversity at nucleotide and Protein levels. Three surface Protein genes were isolated from 45 samples collected in Thailand (N = 33), Myanmar (N = 8), and Lao PDR (N = 4), using conventional polymerase chain reaction (PCR) assay. Then, the PCR products were sequenced and analysed using BioEdit, MEGA6, and DnaSP programs. The average pairwise nucleotide diversities (π) of P. malariae trap, ama1, and P48/45 were 0.00169, 0.00413, and 0.00029, respectively. The haplotype diversities (Hd) of P. malariae trap, ama1, and P48/45 were 0.919, 0.946, and 0.130, respectively. Most of the nucleotide substitutions were non-synonymous, which indicated that the genetic variations of these genes were maintained by positive diversifying selection, thus, suggesting their role as a potential target of protective immune response. Amino acid substitutions of P. malariae TRAP, AMA1, and P48/45 could be categorized to 17, 20, and 2 unique amino-acid variants, respectively. For further vaccine development, carboxyl terminal of P48/45 would be a good candidate according to conserved amino acid at low genetic diversity (π = 0.2–0.3). High mutational diversity was observed in P. malariae trap and ama1 as compared to P48/45 in P. malariae samples isolated from Thailand, Myanmar, and Lao PDR. Taken together, these results suggest that P48/45 might be a good vaccine candidate against P. malariae infection because of its sufficiently low genetic diversity and highly conserved amino acids especially on the carboxyl end.
Truong L X Nguyen - One of the best experts on this subject based on the ideXlab platform.
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long isoform of erbb3 binding Protein P48 mediates Protein kinase b akt dependent hdm2 stabilization and nuclear localization
Experimental Cell Research, 2012Co-Authors: Truong L X Nguyen, Sung Hee UmAbstract:P48 is a long isoform of the ErbB3 binding Protein that has oncogenic functions including promotion of carcinogenesis and induction of malignant transformation through negative regulation of tumor suppressor p53. Here, we show that high level of P48 Protein expression leads to enhance HDM2 phosphorylation by Akt and inhibits the self-ubiquitination of HDM2 by up-regulation of Akt activity, thereby promoting its Protein stability. Moreover, P48 expression leads to accumulated nuclear localization of HDM2, whereas P48 depletion disturbs its nuclear localization. Hence, higher expression of P48 in cancer cells reduces p53 levels through modulation of HDM2 nuclear localization and Protein stability via regulation of its Akt-mediated phosphorylation.
Frank Smithuis - One of the best experts on this subject based on the ideXlab platform.
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Genetic diversity of three surface Protein genes in Plasmodium malariae from three Asian countries
Malaria Journal, 2018Co-Authors: Suttipat Srisutham, Naowarat Saralamba, Kanlaya Sriprawat, Mayfong Mayxay, Frank Smithuis, Francois Nosten, Sasithon Pukrittayakamee, Arjen M. Dondorp, Mallika ImwongAbstract:Background Genetic diversity of the three important antigenic Proteins, namely thrombospondin-related anonymous Protein (TRAP), apical membrane antigen 1 (AMA1), and 6-cysteine Protein (P48/45), all of which are found in various developmental stages of Plasmodium parasites is crucial for targeted vaccine development. While studies related to the genetic diversity of these Proteins are available for Plasmodium falciparum and Plasmodium vivax , barely enough information exists regarding Plasmodium malariae . The present study aims to demonstrate the genetic variations existing among these three genes in P. malariae by analysing their diversity at nucleotide and Protein levels. Methods Three surface Protein genes were isolated from 45 samples collected in Thailand (N = 33), Myanmar (N = 8), and Lao PDR (N = 4), using conventional polymerase chain reaction (PCR) assay. Then, the PCR products were sequenced and analysed using BioEdit, MEGA6, and DnaSP programs. Results The average pairwise nucleotide diversities (π) of P. malariae trap , ama1 , and P48/45 were 0.00169, 0.00413, and 0.00029, respectively. The haplotype diversities (Hd) of P. malariae trap , ama1 , and P48/45 were 0.919, 0.946, and 0.130, respectively. Most of the nucleotide substitutions were non-synonymous, which indicated that the genetic variations of these genes were maintained by positive diversifying selection, thus, suggesting their role as a potential target of protective immune response. Amino acid substitutions of P. malariae TRAP, AMA1, and P48/45 could be categorized to 17, 20, and 2 unique amino-acid variants, respectively. For further vaccine development, carboxyl terminal of P48/45 would be a good candidate according to conserved amino acid at low genetic diversity (π = 0.2–0.3). Conclusions High mutational diversity was observed in P. malariae trap and ama1 as compared to P48/45 in P. malariae samples isolated from Thailand, Myanmar, and Lao PDR. Taken together, these results suggest that P48/45 might be a good vaccine candidate against P. malariae infection because of its sufficiently low genetic diversity and highly conserved amino acids especially on the carboxyl end.
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Genetic diversity of three surface Protein genes in Plasmodium malariae from three Asian countries.
Malaria Journal, 2018Co-Authors: Suttipat Srisutham, Naowarat Saralamba, Kanlaya Sriprawat, Mayfong Mayxay, Frank Smithuis, Francois Nosten, Sasithon Pukrittayakamee, Arjen M. Dondorp, Mallika ImwongAbstract:Genetic diversity of the three important antigenic Proteins, namely thrombospondin-related anonymous Protein (TRAP), apical membrane antigen 1 (AMA1), and 6-cysteine Protein (P48/45), all of which are found in various developmental stages of Plasmodium parasites is crucial for targeted vaccine development. While studies related to the genetic diversity of these Proteins are available for Plasmodium falciparum and Plasmodium vivax, barely enough information exists regarding Plasmodium malariae. The present study aims to demonstrate the genetic variations existing among these three genes in P. malariae by analysing their diversity at nucleotide and Protein levels. Three surface Protein genes were isolated from 45 samples collected in Thailand (N = 33), Myanmar (N = 8), and Lao PDR (N = 4), using conventional polymerase chain reaction (PCR) assay. Then, the PCR products were sequenced and analysed using BioEdit, MEGA6, and DnaSP programs. The average pairwise nucleotide diversities (π) of P. malariae trap, ama1, and P48/45 were 0.00169, 0.00413, and 0.00029, respectively. The haplotype diversities (Hd) of P. malariae trap, ama1, and P48/45 were 0.919, 0.946, and 0.130, respectively. Most of the nucleotide substitutions were non-synonymous, which indicated that the genetic variations of these genes were maintained by positive diversifying selection, thus, suggesting their role as a potential target of protective immune response. Amino acid substitutions of P. malariae TRAP, AMA1, and P48/45 could be categorized to 17, 20, and 2 unique amino-acid variants, respectively. For further vaccine development, carboxyl terminal of P48/45 would be a good candidate according to conserved amino acid at low genetic diversity (π = 0.2–0.3). High mutational diversity was observed in P. malariae trap and ama1 as compared to P48/45 in P. malariae samples isolated from Thailand, Myanmar, and Lao PDR. Taken together, these results suggest that P48/45 might be a good vaccine candidate against P. malariae infection because of its sufficiently low genetic diversity and highly conserved amino acids especially on the carboxyl end.
