The Experts below are selected from a list of 69 Experts worldwide ranked by ideXlab platform
Jordi Mesa - One of the best experts on this subject based on the ideXlab platform.
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Clinical significance of Ret/PTC and p53 Protein expression in sporadic papillary thyroid carcinoma.
Histopathology, 2020Co-Authors: Carles Zafon, Gabriel Obiols, Josep Castellví, Natalia Tallada, Juan Antonio Baena, Rafael Simó, Jordi MesaAbstract:Aims: Rearranged during Transfection (Ret)/papillary thyroid carcinoma (PTC) and p53 are two genes involved in the pathogenesis of PTC. It has been suggested that Ret/PTC expression is associated with higher rates of local extension and lymph node involvement, whereas p53 mutations are more frequent in poorly differentiated and anaplastic carcinomas. In addition, experimental studies have shown that p53 activity can modify the behaviour of PTC carrying Ret/PTC. The aim of this study was to investigate the expression of both Ret/PTC and p53 in order to evaluate their usefulness as prognostic factors. Methods and results: Resected specimens of 61 cases of PTC were studied immunohistochemically using a polyclonal antibody to Ret and a monoclonal antibody to p53 Protein. Ret/PTC expression was associated with extrathyroid extension of PTC, at diagnosis (P
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clinical significance of Ret ptc and p53 Protein expression in sporadic papillary thyroid carcinoma
Histopathology, 2007Co-Authors: Carles Zafon, Gabriel Obiols, Josep Castellví, Natalia Tallada, Juan Antonio Baena, Rafael Simó, Jordi MesaAbstract:Aims: Rearranged during Transfection (Ret)/papillary thyroid carcinoma (PTC) and p53 are two genes involved in the pathogenesis of PTC. It has been suggested that Ret/PTC expression is associated with higher rates of local extension and lymph node involvement, whereas p53 mutations are more frequent in poorly differentiated and anaplastic carcinomas. In addition, experimental studies have shown that p53 activity can modify the behaviour of PTC carrying Ret/PTC. The aim of this study was to investigate the expression of both Ret/PTC and p53 in order to evaluate their usefulness as prognostic factors. Methods and results: Resected specimens of 61 cases of PTC were studied immunohistochemically using a polyclonal antibody to Ret and a monoclonal antibody to p53 Protein. Ret/PTC expression was associated with extrathyroid extension of PTC, at diagnosis (P < 0.05). In contrast, no relationship between p53 immunoreactivity and clinical status was found. In addition, p53 expression was more prevalent among Ret/PTC+ patients, and significantly influenced the relationship observed between Ret/PTC and extrathyroid extension of the disease. Conclusion: Our results suggest that immunohistochemistry for both PTC/Ret and p53 could be useful in the clinical evaluation of patients with PTC.
Enrique Rodriguezboulan - One of the best experts on this subject based on the ideXlab platform.
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identification of the Retinal pigment epithelium Protein Ret pe2 as ce 9 ox 47 a member of the immunoglobulin superfamily
Investigative Ophthalmology & Visual Science, 1997Co-Authors: Silvia C Finnemann, Alan D Marmorstein, James M Neill, Enrique RodriguezboulanAbstract:Purpose. To identify the Retinal pigment epithelium (RPE) surface antigen recognized by the monoclonal antibody Ret-PE2. Methods. A lambda bacteriophage complementary DNA (cDNA) expression library, representing the rat RPE cell line RPE-J, was constructed and screened with the Ret-PE2 monoclonal antibody. Transient transfections of the Ret-PE2 cDNA, immunofluorescence stainings of tissue sections or cultured cells, and Western blot analyses of tissue and cell detergent extracts served to prove that the Protein resulting from expression of the cDNA is the Ret-PE2 antigen. Results. Three independent cDNAs were cloned that shared overlapping sequences. Sequence alignment with EMBL database entries revealed identity to the published cDNA of CE-9/OX-47, a member of the immunoglobulin superfamily. One of the clones encoded the entire open reading frame of CE-9. The expression pattern of the Ret-PE2 antigen matched that of CE-9, which is widely expressed. Chinese hamster ovary cells transiently transfected with the Ret-PE2 cDNA produced a membrane-localized Protein that was recognized by Ret-PE2 and CE-9 antibodies. Conclusions. The antibody Ret-PE2 recognizes the CE-9/OX-47 gene product, a transmembrane Protein of the immunoglobulin superfamily. Contrary to results reported earlier, Ret-PE2 immunoreactivity is widely distributed among different rat tissues-kidney, liver, and testis. In epithelia other than the adult RPE, it is confined to the basolateral plasma membrane. Its apical polarization in the RPE of adult rats supports earlier findings that some Proteins that are basolateral in other epithelia exhibit reversed polarity in the RPE.
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the polarity of the plasma membrane Protein Ret pe2 in Retinal pigment epithelium is developmentally regulated
Journal of Cell Science, 1996Co-Authors: Alan D Marmorstein, Vera L Bonilha, Silvia Chiflet, James M Neill, Enrique RodriguezboulanAbstract:The Retinal pigment epithelium (RPE) differs from other epithelia in that the apical surface is not free; instead, it interacts with both photoreceptors and a specialized extracellular material, the interphotoreceptor matrix. Biochemical characterization of the apical and basolateral surfaces of RPE in adult rat eye cups, using a novel in situ biotinylation assay, revealed very different Protein compositions and identified a major surface antigen, Ret-PE2, with a predominantly apical distribution (approximately 74%). The apical polarity of Ret-PE2 was confirmed by immunofluorescence and laser scanning confocal microscopy. In striking contrast, Ret-PE2 antigen was preferentially basolateral in primary cultures derived from adult rat RPE and in an immortalized RPE cell line (RPE-J). Under all conditions, Ret-PE2 was highly soluble in Triton X-100 (> 81% at 4 degrees C), suggesting that its redistribution was not dependent on changes in cytoskeletal interactions. Analysis of the localization of Ret-PE2 in normal rats at postnatal (PN) days 1, 7, and 14 indicated that Ret-PE2 redistributes from predominantly basolateral to predominantly apical during that time. Since photoreceptors develop during the first two weeks after birth in the rat, our results suggest that the apical redistribution of Ret-PE2 is dependent on the establishment of adult interactions between the RPE and the neural Retina and/or the interphotoreceptor matrix, either via direct contacts or through alterations in the intracellular sorting patterns of RPE cells.
Carles Zafon - One of the best experts on this subject based on the ideXlab platform.
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Clinical significance of Ret/PTC and p53 Protein expression in sporadic papillary thyroid carcinoma.
Histopathology, 2020Co-Authors: Carles Zafon, Gabriel Obiols, Josep Castellví, Natalia Tallada, Juan Antonio Baena, Rafael Simó, Jordi MesaAbstract:Aims: Rearranged during Transfection (Ret)/papillary thyroid carcinoma (PTC) and p53 are two genes involved in the pathogenesis of PTC. It has been suggested that Ret/PTC expression is associated with higher rates of local extension and lymph node involvement, whereas p53 mutations are more frequent in poorly differentiated and anaplastic carcinomas. In addition, experimental studies have shown that p53 activity can modify the behaviour of PTC carrying Ret/PTC. The aim of this study was to investigate the expression of both Ret/PTC and p53 in order to evaluate their usefulness as prognostic factors. Methods and results: Resected specimens of 61 cases of PTC were studied immunohistochemically using a polyclonal antibody to Ret and a monoclonal antibody to p53 Protein. Ret/PTC expression was associated with extrathyroid extension of PTC, at diagnosis (P
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clinical significance of Ret ptc and p53 Protein expression in sporadic papillary thyroid carcinoma
Histopathology, 2007Co-Authors: Carles Zafon, Gabriel Obiols, Josep Castellví, Natalia Tallada, Juan Antonio Baena, Rafael Simó, Jordi MesaAbstract:Aims: Rearranged during Transfection (Ret)/papillary thyroid carcinoma (PTC) and p53 are two genes involved in the pathogenesis of PTC. It has been suggested that Ret/PTC expression is associated with higher rates of local extension and lymph node involvement, whereas p53 mutations are more frequent in poorly differentiated and anaplastic carcinomas. In addition, experimental studies have shown that p53 activity can modify the behaviour of PTC carrying Ret/PTC. The aim of this study was to investigate the expression of both Ret/PTC and p53 in order to evaluate their usefulness as prognostic factors. Methods and results: Resected specimens of 61 cases of PTC were studied immunohistochemically using a polyclonal antibody to Ret and a monoclonal antibody to p53 Protein. Ret/PTC expression was associated with extrathyroid extension of PTC, at diagnosis (P < 0.05). In contrast, no relationship between p53 immunoreactivity and clinical status was found. In addition, p53 expression was more prevalent among Ret/PTC+ patients, and significantly influenced the relationship observed between Ret/PTC and extrathyroid extension of the disease. Conclusion: Our results suggest that immunohistochemistry for both PTC/Ret and p53 could be useful in the clinical evaluation of patients with PTC.
Alan D Marmorstein - One of the best experts on this subject based on the ideXlab platform.
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identification of the Retinal pigment epithelium Protein Ret pe2 as ce 9 ox 47 a member of the immunoglobulin superfamily
Investigative Ophthalmology & Visual Science, 1997Co-Authors: Silvia C Finnemann, Alan D Marmorstein, James M Neill, Enrique RodriguezboulanAbstract:Purpose. To identify the Retinal pigment epithelium (RPE) surface antigen recognized by the monoclonal antibody Ret-PE2. Methods. A lambda bacteriophage complementary DNA (cDNA) expression library, representing the rat RPE cell line RPE-J, was constructed and screened with the Ret-PE2 monoclonal antibody. Transient transfections of the Ret-PE2 cDNA, immunofluorescence stainings of tissue sections or cultured cells, and Western blot analyses of tissue and cell detergent extracts served to prove that the Protein resulting from expression of the cDNA is the Ret-PE2 antigen. Results. Three independent cDNAs were cloned that shared overlapping sequences. Sequence alignment with EMBL database entries revealed identity to the published cDNA of CE-9/OX-47, a member of the immunoglobulin superfamily. One of the clones encoded the entire open reading frame of CE-9. The expression pattern of the Ret-PE2 antigen matched that of CE-9, which is widely expressed. Chinese hamster ovary cells transiently transfected with the Ret-PE2 cDNA produced a membrane-localized Protein that was recognized by Ret-PE2 and CE-9 antibodies. Conclusions. The antibody Ret-PE2 recognizes the CE-9/OX-47 gene product, a transmembrane Protein of the immunoglobulin superfamily. Contrary to results reported earlier, Ret-PE2 immunoreactivity is widely distributed among different rat tissues-kidney, liver, and testis. In epithelia other than the adult RPE, it is confined to the basolateral plasma membrane. Its apical polarization in the RPE of adult rats supports earlier findings that some Proteins that are basolateral in other epithelia exhibit reversed polarity in the RPE.
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the polarity of the plasma membrane Protein Ret pe2 in Retinal pigment epithelium is developmentally regulated
Journal of Cell Science, 1996Co-Authors: Alan D Marmorstein, Vera L Bonilha, Silvia Chiflet, James M Neill, Enrique RodriguezboulanAbstract:The Retinal pigment epithelium (RPE) differs from other epithelia in that the apical surface is not free; instead, it interacts with both photoreceptors and a specialized extracellular material, the interphotoreceptor matrix. Biochemical characterization of the apical and basolateral surfaces of RPE in adult rat eye cups, using a novel in situ biotinylation assay, revealed very different Protein compositions and identified a major surface antigen, Ret-PE2, with a predominantly apical distribution (approximately 74%). The apical polarity of Ret-PE2 was confirmed by immunofluorescence and laser scanning confocal microscopy. In striking contrast, Ret-PE2 antigen was preferentially basolateral in primary cultures derived from adult rat RPE and in an immortalized RPE cell line (RPE-J). Under all conditions, Ret-PE2 was highly soluble in Triton X-100 (> 81% at 4 degrees C), suggesting that its redistribution was not dependent on changes in cytoskeletal interactions. Analysis of the localization of Ret-PE2 in normal rats at postnatal (PN) days 1, 7, and 14 indicated that Ret-PE2 redistributes from predominantly basolateral to predominantly apical during that time. Since photoreceptors develop during the first two weeks after birth in the rat, our results suggest that the apical redistribution of Ret-PE2 is dependent on the establishment of adult interactions between the RPE and the neural Retina and/or the interphotoreceptor matrix, either via direct contacts or through alterations in the intracellular sorting patterns of RPE cells.
Juan Antonio Baena - One of the best experts on this subject based on the ideXlab platform.
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Clinical significance of Ret/PTC and p53 Protein expression in sporadic papillary thyroid carcinoma.
Histopathology, 2020Co-Authors: Carles Zafon, Gabriel Obiols, Josep Castellví, Natalia Tallada, Juan Antonio Baena, Rafael Simó, Jordi MesaAbstract:Aims: Rearranged during Transfection (Ret)/papillary thyroid carcinoma (PTC) and p53 are two genes involved in the pathogenesis of PTC. It has been suggested that Ret/PTC expression is associated with higher rates of local extension and lymph node involvement, whereas p53 mutations are more frequent in poorly differentiated and anaplastic carcinomas. In addition, experimental studies have shown that p53 activity can modify the behaviour of PTC carrying Ret/PTC. The aim of this study was to investigate the expression of both Ret/PTC and p53 in order to evaluate their usefulness as prognostic factors. Methods and results: Resected specimens of 61 cases of PTC were studied immunohistochemically using a polyclonal antibody to Ret and a monoclonal antibody to p53 Protein. Ret/PTC expression was associated with extrathyroid extension of PTC, at diagnosis (P
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clinical significance of Ret ptc and p53 Protein expression in sporadic papillary thyroid carcinoma
Histopathology, 2007Co-Authors: Carles Zafon, Gabriel Obiols, Josep Castellví, Natalia Tallada, Juan Antonio Baena, Rafael Simó, Jordi MesaAbstract:Aims: Rearranged during Transfection (Ret)/papillary thyroid carcinoma (PTC) and p53 are two genes involved in the pathogenesis of PTC. It has been suggested that Ret/PTC expression is associated with higher rates of local extension and lymph node involvement, whereas p53 mutations are more frequent in poorly differentiated and anaplastic carcinomas. In addition, experimental studies have shown that p53 activity can modify the behaviour of PTC carrying Ret/PTC. The aim of this study was to investigate the expression of both Ret/PTC and p53 in order to evaluate their usefulness as prognostic factors. Methods and results: Resected specimens of 61 cases of PTC were studied immunohistochemically using a polyclonal antibody to Ret and a monoclonal antibody to p53 Protein. Ret/PTC expression was associated with extrathyroid extension of PTC, at diagnosis (P < 0.05). In contrast, no relationship between p53 immunoreactivity and clinical status was found. In addition, p53 expression was more prevalent among Ret/PTC+ patients, and significantly influenced the relationship observed between Ret/PTC and extrathyroid extension of the disease. Conclusion: Our results suggest that immunohistochemistry for both PTC/Ret and p53 could be useful in the clinical evaluation of patients with PTC.
