The Experts below are selected from a list of 182265 Experts worldwide ranked by ideXlab platform
Takashi Shirai - One of the best experts on this subject based on the ideXlab platform.
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serum Protein adsorption and platelet adhesion on pluronic adsorbed polysulfone membranes
Biomaterials, 2003Co-Authors: Akon Higuchi, Kaichiro Sugiyama, Boo Ok Yoon, Masaya Sumita, Shuichi Sugawara, Mariko Hara, Masaru Sakurai, Takashi ShiraiAbstract:We examined plasma Protein adsorption and platelet adhesion to polysulfone (PSf) flat membranes coated with Pluronic™ with varying polyethylene oxide (PEO) block length. Adsorption of albumin, globulin and fibrinogen to Pluronic™-coated PSf membranes was independent of plasma dilution when concentrations of human blood plasma above 20% were applied. Increasing coating concentrations of aqueous Pluronic™ Solution resulted in decreased Protein adsorption by the PSf membranes. Pluronic™ F68, which was more hydrophilic than Pluronic™ L62 or L64 and had 80% of PEO content, was the most effective at suppressing the adsorption of plasma Proteins and platelet adhesion to PSf membranes. We developed a mixed Protein Solution containing human albumin, γ-globulin and fibrinogen to attempt to mimic the competitive and cooperative binding effects found in plasma. Fibrinogen adsorption from plasma could be recapitulated by the mixed Protein Solution. The number of platelets adhering to the PSf membranes decreased as the coating concentration of Pluronic™ Solution was increased, and platelet adhesion decreased in parallel with fibrinogen adsorption. These results suggest that the bioinert property of PEO segments in the Pluronic™, which is ascribed to their high flexibility in aqueous media, suppresses the adsorption of plasma Proteins and platelets to the Pluronic™-coated PSf membranes.
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serum Protein adsorption and platelet adhesion on pluronic adsorbed polysulfone membranes
Biomaterials, 2003Co-Authors: Akon Higuchi, Kaichiro Sugiyama, Boo Ok Yoon, Masaya Sumita, Shuichi Sugawara, Mariko Hara, Masaru Sakurai, Takashi ShiraiAbstract:We examined plasma Protein adsorption and platelet adhesion to polysulfone (PSf) flat membranes coated with Pluronic™ with varying polyethylene oxide (PEO) block length. Adsorption of albumin, globulin and fibrinogen to Pluronic™-coated PSf membranes was independent of plasma dilution when concentrations of human blood plasma above 20% were applied. Increasing coating concentrations of aqueous Pluronic™ Solution resulted in decreased Protein adsorption by the PSf membranes. Pluronic™ F68, which was more hydrophilic than Pluronic™ L62 or L64 and had 80% of PEO content, was the most effective at suppressing the adsorption of plasma Proteins and platelet adhesion to PSf membranes. We developed a mixed Protein Solution containing human albumin, γ-globulin and fibrinogen to attempt to mimic the competitive and cooperative binding effects found in plasma. Fibrinogen adsorption from plasma could be recapitulated by the mixed Protein Solution. The number of platelets adhering to the PSf membranes decreased as the coating concentration of Pluronic™ Solution was increased, and platelet adhesion decreased in parallel with fibrinogen adsorption. These results suggest that the bioinert property of PEO segments in the Pluronic™, which is ascribed to their high flexibility in aqueous media, suppresses the adsorption of plasma Proteins and platelets to the Pluronic™-coated PSf membranes.
Akon Higuchi - One of the best experts on this subject based on the ideXlab platform.
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serum Protein adsorption and platelet adhesion on pluronic adsorbed polysulfone membranes
Biomaterials, 2003Co-Authors: Akon Higuchi, Kaichiro Sugiyama, Boo Ok Yoon, Masaya Sumita, Shuichi Sugawara, Mariko Hara, Masaru Sakurai, Takashi ShiraiAbstract:We examined plasma Protein adsorption and platelet adhesion to polysulfone (PSf) flat membranes coated with Pluronic™ with varying polyethylene oxide (PEO) block length. Adsorption of albumin, globulin and fibrinogen to Pluronic™-coated PSf membranes was independent of plasma dilution when concentrations of human blood plasma above 20% were applied. Increasing coating concentrations of aqueous Pluronic™ Solution resulted in decreased Protein adsorption by the PSf membranes. Pluronic™ F68, which was more hydrophilic than Pluronic™ L62 or L64 and had 80% of PEO content, was the most effective at suppressing the adsorption of plasma Proteins and platelet adhesion to PSf membranes. We developed a mixed Protein Solution containing human albumin, γ-globulin and fibrinogen to attempt to mimic the competitive and cooperative binding effects found in plasma. Fibrinogen adsorption from plasma could be recapitulated by the mixed Protein Solution. The number of platelets adhering to the PSf membranes decreased as the coating concentration of Pluronic™ Solution was increased, and platelet adhesion decreased in parallel with fibrinogen adsorption. These results suggest that the bioinert property of PEO segments in the Pluronic™, which is ascribed to their high flexibility in aqueous media, suppresses the adsorption of plasma Proteins and platelets to the Pluronic™-coated PSf membranes.
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serum Protein adsorption and platelet adhesion on pluronic adsorbed polysulfone membranes
Biomaterials, 2003Co-Authors: Akon Higuchi, Kaichiro Sugiyama, Boo Ok Yoon, Masaya Sumita, Shuichi Sugawara, Mariko Hara, Masaru Sakurai, Takashi ShiraiAbstract:We examined plasma Protein adsorption and platelet adhesion to polysulfone (PSf) flat membranes coated with Pluronic™ with varying polyethylene oxide (PEO) block length. Adsorption of albumin, globulin and fibrinogen to Pluronic™-coated PSf membranes was independent of plasma dilution when concentrations of human blood plasma above 20% were applied. Increasing coating concentrations of aqueous Pluronic™ Solution resulted in decreased Protein adsorption by the PSf membranes. Pluronic™ F68, which was more hydrophilic than Pluronic™ L62 or L64 and had 80% of PEO content, was the most effective at suppressing the adsorption of plasma Proteins and platelet adhesion to PSf membranes. We developed a mixed Protein Solution containing human albumin, γ-globulin and fibrinogen to attempt to mimic the competitive and cooperative binding effects found in plasma. Fibrinogen adsorption from plasma could be recapitulated by the mixed Protein Solution. The number of platelets adhering to the PSf membranes decreased as the coating concentration of Pluronic™ Solution was increased, and platelet adhesion decreased in parallel with fibrinogen adsorption. These results suggest that the bioinert property of PEO segments in the Pluronic™, which is ascribed to their high flexibility in aqueous media, suppresses the adsorption of plasma Proteins and platelets to the Pluronic™-coated PSf membranes.
Jennifer E Woodellmay - One of the best experts on this subject based on the ideXlab platform.
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white blood cell concentration correlates with increased concentrations of il 1ra and improvement in womac pain scores in an open label safety study of autologous Protein Solution
Journal of Experimental Orthopaedics, 2016Co-Authors: William P King, Walter Van Der Weegen, Rogier A M Van Drumpt, Hans Soons, Krista Toler, Jennifer E WoodellmayAbstract:Background There has been debate on which blood components should be included in autologous therapies. Autologous Protein Solution (APS) is a unique blood-derived therapy composed of concentrated white blood cells, platelets, and plasma to contain high concentrations of anti-inflammatory cytokines and anabolic growth factors to potentially address osteoarthritis. The primary aim of the exploratory secondary analysis was to identify characteristics of an Autologous Protein Solution (APS) that may correlate with improved Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) scores and OMERACT-OARSI responder status after treatment of subjects with an intra-articular injection of APS.
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autologous Protein Solution prepared from the blood of osteoarthritic patients contains an enhanced profile of anti inflammatory cytokines and anabolic growth factors
Journal of Orthopaedic Research, 2014Co-Authors: Krista Oshaughnessey, Andrea M Matuska, Jacy C Hoeppner, William P King, Jack Farr, Mark Klaassen, Christopher C Kaeding, Christian Lattermann, Jennifer E WoodellmayAbstract:The objective of this clinical study was to test if blood from osteoarthritis (OA) patients (n = 105) could be processed by a device system to form an autologous Protein Solution (APS) with preferentially increased concentrations of anti-inflammatory cytokines compared to inflammatory cytokines. To address this objective, APS was prepared from patients exhibiting radiographic evidence of knee OA. Patient metrics were collected including: demographic information, medical history, medication records, and Knee Injury and Osteoarthritis Outcome Score (KOOS) surveys. Cytokine and growth factor concentrations in whole blood and APS were measured using enzyme-linked immunosorbent assays. Statistical analyses were used to identify relationships between OA patient metrics and cytokines. The results of this study indicated that anti-inflammatory cytokines were preferentially increased compared to inflammatory cytokines in APS from 98% of OA patients. APS contained high concentrations of anti-inflammatory Proteins including 39,000 ± 20,000 pg/ml IL-1ra, 21,000 ± 5,000 pg/ml sIL-1RII, 2,100 ± 570 pg/ml sTNF-RI, and 4,200 ± 1,500 pg/ml sTNF-RII. Analysis of the 82 patient metrics indicated that no single patient metric was strongly correlated (R2 > 0.7) with the key cytokine concentrations in APS. Therefore, APS can be prepared from a broad range of OA patients. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:1349–1355, 2014.
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autologous Protein Solution inhibits mmp 13 production by il 1β and tnfα stimulated human articular chondrocytes
Journal of Orthopaedic Research, 2011Co-Authors: Jennifer E Woodellmay, Andrea M Matuska, Megan Oyster, Zachary R Welch, Krista Oshaughnessey, Jacy C HoeppnerAbstract:Catabolic inflammatory cytokines are prevalent in osteoarthritis (OA). The purpose of this study was to evaluate an autologous Protein Solution (APS) as a potential chondroprotective agent for OA therapy. APS was prepared from platelet-rich plasma (PRP). The APS Solution contained both anabolic (bFGF, TGF-β1, TGF-β2, EGF, IGF-1, PDGF-AB, PDGF-BB, and VEGF) and anti-inflammatory (IL-1ra, sTNF-RI, sTNF-RII, IL-4, IL-10, IL-13, and IFNγ) cytokines but low concentrations of catabolic cytokines (IL-1α, IL-1β, TNFα, IL-6, IL-8, IL-17, and IL-18). Human articular chondrocytes were pre-incubated with the antagonists IL-1ra, sTNF-RI, or APS prior to the addition of recombinant human IL-1β or TNFα. Following exposure to inflammatory cytokines, the levels of MMP-13 in the culture medium were evaluated by ELISA. MMP-13 production stimulated in chondrocytes by IL-1β or TNFα was reduced by rhIL-1ra and sTNF-RI to near basal levels. APS was also capable of inhibiting the production of MMP-13 induced by both IL-1β and TNFα. The combination of anabolic and anti-inflammatory cytokines in the APS created from PRP may render this formulation to be a potential candidate for the treatment of inflammation in patients at early stages of OA. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 29: 1320–1326, 2011
Jacy C Hoeppner - One of the best experts on this subject based on the ideXlab platform.
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autologous Protein Solution prepared from the blood of osteoarthritic patients contains an enhanced profile of anti inflammatory cytokines and anabolic growth factors
Journal of Orthopaedic Research, 2014Co-Authors: Krista Oshaughnessey, Andrea M Matuska, Jacy C Hoeppner, William P King, Jack Farr, Mark Klaassen, Christopher C Kaeding, Christian Lattermann, Jennifer E WoodellmayAbstract:The objective of this clinical study was to test if blood from osteoarthritis (OA) patients (n = 105) could be processed by a device system to form an autologous Protein Solution (APS) with preferentially increased concentrations of anti-inflammatory cytokines compared to inflammatory cytokines. To address this objective, APS was prepared from patients exhibiting radiographic evidence of knee OA. Patient metrics were collected including: demographic information, medical history, medication records, and Knee Injury and Osteoarthritis Outcome Score (KOOS) surveys. Cytokine and growth factor concentrations in whole blood and APS were measured using enzyme-linked immunosorbent assays. Statistical analyses were used to identify relationships between OA patient metrics and cytokines. The results of this study indicated that anti-inflammatory cytokines were preferentially increased compared to inflammatory cytokines in APS from 98% of OA patients. APS contained high concentrations of anti-inflammatory Proteins including 39,000 ± 20,000 pg/ml IL-1ra, 21,000 ± 5,000 pg/ml sIL-1RII, 2,100 ± 570 pg/ml sTNF-RI, and 4,200 ± 1,500 pg/ml sTNF-RII. Analysis of the 82 patient metrics indicated that no single patient metric was strongly correlated (R2 > 0.7) with the key cytokine concentrations in APS. Therefore, APS can be prepared from a broad range of OA patients. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:1349–1355, 2014.
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autologous Protein Solution inhibits mmp 13 production by il 1β and tnfα stimulated human articular chondrocytes
Journal of Orthopaedic Research, 2011Co-Authors: Jennifer E Woodellmay, Andrea M Matuska, Megan Oyster, Zachary R Welch, Krista Oshaughnessey, Jacy C HoeppnerAbstract:Catabolic inflammatory cytokines are prevalent in osteoarthritis (OA). The purpose of this study was to evaluate an autologous Protein Solution (APS) as a potential chondroprotective agent for OA therapy. APS was prepared from platelet-rich plasma (PRP). The APS Solution contained both anabolic (bFGF, TGF-β1, TGF-β2, EGF, IGF-1, PDGF-AB, PDGF-BB, and VEGF) and anti-inflammatory (IL-1ra, sTNF-RI, sTNF-RII, IL-4, IL-10, IL-13, and IFNγ) cytokines but low concentrations of catabolic cytokines (IL-1α, IL-1β, TNFα, IL-6, IL-8, IL-17, and IL-18). Human articular chondrocytes were pre-incubated with the antagonists IL-1ra, sTNF-RI, or APS prior to the addition of recombinant human IL-1β or TNFα. Following exposure to inflammatory cytokines, the levels of MMP-13 in the culture medium were evaluated by ELISA. MMP-13 production stimulated in chondrocytes by IL-1β or TNFα was reduced by rhIL-1ra and sTNF-RI to near basal levels. APS was also capable of inhibiting the production of MMP-13 induced by both IL-1β and TNFα. The combination of anabolic and anti-inflammatory cytokines in the APS created from PRP may render this formulation to be a potential candidate for the treatment of inflammation in patients at early stages of OA. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 29: 1320–1326, 2011
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Autologous Protein Solution inhibits MMP-13 production by IL-1β and TNFα-stimulated human articular chondrocytes.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 2011Co-Authors: Jennifer E. Woodell-may, Andrea M Matuska, Megan Oyster, Zachary R Welch, Krista O'shaughnessey, Jacy C HoeppnerAbstract:Catabolic inflammatory cytokines are prevalent in osteoarthritis (OA). The purpose of this study was to evaluate an autologous Protein Solution (APS) as a potential chondroprotective agent for OA therapy. APS was prepared from platelet-rich plasma (PRP). The APS Solution contained both anabolic (bFGF, TGF-β1, TGF-β2, EGF, IGF-1, PDGF-AB, PDGF-BB, and VEGF) and anti-inflammatory (IL-1ra, sTNF-RI, sTNF-RII, IL-4, IL-10, IL-13, and IFNγ) cytokines but low concentrations of catabolic cytokines (IL-1α, IL-1β, TNFα, IL-6, IL-8, IL-17, and IL-18). Human articular chondrocytes were pre-incubated with the antagonists IL-1ra, sTNF-RI, or APS prior to the addition of recombinant human IL-1β or TNFα. Following exposure to inflammatory cytokines, the levels of MMP-13 in the culture medium were evaluated by ELISA. MMP-13 production stimulated in chondrocytes by IL-1β or TNFα was reduced by rhIL-1ra and sTNF-RI to near basal levels. APS was also capable of inhibiting the production of MMP-13 induced by both IL-1β and TNFα. The combination of anabolic and anti-inflammatory cytokines in the APS created from PRP may render this formulation to be a potential candidate for the treatment of inflammation in patients at early stages of OA. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 29: 1320–1326, 2011
Yongkeun Park - One of the best experts on this subject based on the ideXlab platform.
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measurements of complex refractive index change of photoactive yellow Protein over a wide wavelength range using hyperspectral quantitative phase imaging
Scientific Reports, 2018Co-Authors: Kyeoreh Lee, Youngmin Kim, Jaehwang Jung, Hyotcherl Ihee, Yongkeun ParkAbstract:A novel optical holographic technique is presented to simultaneously measure both the real and imaginary components of the complex refractive index (CRI) of a Protein Solution over a wide visible wavelength range. Quantitative phase imaging was employed to precisely measure the optical field transmitted from a Protein Solution, from which the CRIs of the Protein Solution were retrieved using the Fourier light scattering technique. Using this method, we characterized the CRIs of the two dominant structural states of a photoactive yellow Protein Solution over a broad wavelength range (461–582 nm). The significant CRI deviation between the two structural states was quantified and analysed. The results of both states show the similar overall shape of the expected rRI obtained from the Kramers–Kronig relations.
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Measurements of complex refractive index change of photoactive yellow Protein over a wide wavelength range using hyperspectral quantitative phase imaging
NATURE PUBLISHING GROUP, 2018Co-Authors: Kyereh Lee, Youngmin Kim, Jaehwang Jung, Hyotcherl Ihee, Yongkeun ParkAbstract:A novel optical holographic technique is presented to simultaneously measure both the real and imaginary components of the complex refractive index (CRI) of a Protein Solution over a wide visible wavelength range. Quantitative phase imaging was employed to precisely measure the optical field transmitted from a Protein Solution, from which the CRIs of the Protein Solution were retrieved using the Fourier light scattering technique. Using this method, we characterized the CRIs of the two dominant structural states of a photoactive yellow Protein Solution over a broad wavelength range (461-582 nm). The significant CRI deviation between the two structural states was quantified and analysed. The results of both states show the similar overall shape of the expected rRI obtained from the Kramers-Kronig relations. © 2018 The Author(s
