The Experts below are selected from a list of 663 Experts worldwide ranked by ideXlab platform
Jianping Chen - One of the best experts on this subject based on the ideXlab platform.
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characterization of steroidal saponins in crude extract from dioscorea nipponica makino by liquid chromatography tandem multi stage mass spectrometry
Analytica Chimica Acta, 2007Co-Authors: Dongmei Wang, Depo Yang, Yao Tong, Jianping ChenAbstract:Abstract The liquid chromatography–electrospray ionization-tandem multi-stage mass spectrometry (LC–ESI-MS n ) method was developed for the analyses and characterization of steroidal saponins in plant extract from the rhizome of Dioscorea nipponica Makino. The HPLC experiments were performed by means of a reversed-phase C 18 column and a binary mobile phase system consisting of water and acetonitrile under gradient elution conditions. PseudoProtodioscin, methyl Protodioscin and dioscin were identified by comparing the retention times, UV spectra and the fragmentation properties of [ M − H] − ions with the authentic standards. Four groups of steroidal saponin isomers possessed the [ M − H] − ions at m / z 1063, 1045, 901 and 1047, respectively, were observed during the LC–ESI(−)-MS analysis, and three groups of them except the pair of isomers with the [ M − H] − ions at m / z 1047 could be differentiated by LC–ESI(−)-MS 3 . Furthermore, the ESI-MS n fragmentation behaviors of the [ M + Li] + ions of pseudoProtodioscin and methyl Protodioscin have been investigated, and the observed information helped the structural elucidation of the more abundant isomer with the [ M − H] − ion at m / z 1047. As the result, a special sugar sequence of the saccharide chains was observed that not glucose but rhamnose might be connected with the hydroxyl group at C-3 position of the steroidal aglycone.
Dongmei Wang - One of the best experts on this subject based on the ideXlab platform.
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characterization of steroidal saponins in crude extract from dioscorea nipponica makino by liquid chromatography tandem multi stage mass spectrometry
Analytica Chimica Acta, 2007Co-Authors: Dongmei Wang, Depo Yang, Yao Tong, Jianping ChenAbstract:Abstract The liquid chromatography–electrospray ionization-tandem multi-stage mass spectrometry (LC–ESI-MS n ) method was developed for the analyses and characterization of steroidal saponins in plant extract from the rhizome of Dioscorea nipponica Makino. The HPLC experiments were performed by means of a reversed-phase C 18 column and a binary mobile phase system consisting of water and acetonitrile under gradient elution conditions. PseudoProtodioscin, methyl Protodioscin and dioscin were identified by comparing the retention times, UV spectra and the fragmentation properties of [ M − H] − ions with the authentic standards. Four groups of steroidal saponin isomers possessed the [ M − H] − ions at m / z 1063, 1045, 901 and 1047, respectively, were observed during the LC–ESI(−)-MS analysis, and three groups of them except the pair of isomers with the [ M − H] − ions at m / z 1047 could be differentiated by LC–ESI(−)-MS 3 . Furthermore, the ESI-MS n fragmentation behaviors of the [ M + Li] + ions of pseudoProtodioscin and methyl Protodioscin have been investigated, and the observed information helped the structural elucidation of the more abundant isomer with the [ M − H] − ion at m / z 1047. As the result, a special sugar sequence of the saccharide chains was observed that not glucose but rhamnose might be connected with the hydroxyl group at C-3 position of the steroidal aglycone.
Pawan Kumar - One of the best experts on this subject based on the ideXlab platform.
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A novel Protodioscin-enriched fenugreek seed extract (Trigonella foenum-graecum, family Fabaceae) improves free testosterone level and sperm profile in healthy volunteers
Functional Foods in Health and Disease, 2017Co-Authors: Debasis Bagchi, Anand Swaroop, Manashi Bagchi, Harry G. Preuss, Anuj Maheshwari, Narsingh Verma, Kiran Tiwari, Pawan KumarAbstract:Bac kground : A novel, patent-pending 20% Protodioscin-enriched extract has been developed in our laboratories from fenugreek seeds (Furosap ® ). We assessed the efficacy of Furosap ® in 50 male volunteers (age: 35-65 years) on free and total increased testosterone levels, sperm profile, mental alertness, cardiovascular health, mood, libido, and quality of life. Methods: Furosap ® (500 mg/day/subject) was administered to the 50 male volunteers over a period of 12 weeks in a one-arm, open-labelled study, to determine the efficacy on free and total testosterone levels, sperm profile and sperm morphology, libido and erectile dysfunction, mood and mental alertness and broad spectrum safety parameters. Institution Review Board approval was obtained for this study and the study was registered at the clinicaltrials.gov (NCT02702882). Results: A statistically significant increase in free testosterone levels were observed in these volunteers following supplementation of Furosap ® . Sperm morphology, sperm counts, mental alertness, mood, cardiovascular health, and libido performance were significantly improved. Extensive blood chemistry analyses revealed broad spectrum safety. No significant changes were observed in serum lipid function, cholesterol, triglyceride, HDL and LDL levels, and hemogram. Conclusions: Results confirmed that this Protodioscin-enriched extract from fenugreek seeds (Furosap) is safe and efficacious in boosting serum free testosterone levels, healthy sperm profile, mental alertness, cardiovascular health, and overall performance in male volunteers. Keywords: Standardized fenugreek seed extract; Protodioscin; Testosterone booster; Sperm profile; Mood alleviation; Safety
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A novel Protodioscin-enriched fenugreek seed extract (Trigonella foenum-graecum, family Fabaceae) improves free testosterone level and sperm profile in healthy volunteers
Food Science Publisher, 2017Co-Authors: Debasis Bagchi, Anand Swaroop, Manashi Bagchi, Harry G. Preuss, Anuj Maheshwari, Narsingh Verma, Kiran Tiwari, Pawan KumarAbstract:Background: A novel, patent-pending 20% Protodioscin-enriched extract has been developed in our laboratories from fenugreek seeds(Furosap®). We assessed the efficacy of Furosap®in 50 male volunteers (age: 35-65 years)on free and total increased testosterone levels, sperm profile, mental alertness, cardiovascular health, mood, libido,and quality of life. Methods: Furosap®(500 mg/day/subject)was administered to the 50 male volunteers over a period of 12 weeks in aone-arm, open-labelled study, to determine the efficacy on free and total testosterone levels, sperm profile andsperm morphology, libido and erectile dysfunction, mood and mental alertness and broad spectrum safety parameters. Institution Review Board approval was obtained for this study and the study was registered at the clinicaltrials.gov(NCT02702882).Results: A statistically significant increase in freetestosterone levels were observed in these volunteers following supplementation of Furosap®. Sperm morphology, sperm counts, mental alertness, mood, cardiovascular health,and libidoperformancewere significantly improved. Extensive blood chemistry analyses revealed broad spectrum safety. No significant changes were observed in serum lipid function, cholesterol, triglyceride, HDL and LDLlevels,andhemogram.Conclusions: Results confirmedthat this Protodioscin-enriched extract from fenugreek seeds (Furosap)is safe and efficacious in boosting serum free testosterone levels, healthy sperm profile, mental alertness, cardiovascular health,and overall performance in male volunteer
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Efficacy of Furosap, a novel Trigonella foenum-graecum seed extract, in Enhancing Healthy Testosterone Level and Improving Sperm Profile in Male Volunteers
The FASEB Journal, 2016Co-Authors: Anand Swaroop, Debasis Bagchi, Manashi Bagchi, Harry G. Preuss, Pawan KumarAbstract:Furosap, an innovative 20% Protodioscin-enriched extract derived from Trigonella foenum-graecum seeds, was developed in our laboratory, which effectively increased testosterone levels, sperm profil...
Debasis Bagchi - One of the best experts on this subject based on the ideXlab platform.
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A novel Protodioscin-enriched fenugreek seed extract (Trigonella foenum-graecum, family Fabaceae) improves free testosterone level and sperm profile in healthy volunteers
Functional Foods in Health and Disease, 2017Co-Authors: Debasis Bagchi, Anand Swaroop, Manashi Bagchi, Harry G. Preuss, Anuj Maheshwari, Narsingh Verma, Kiran Tiwari, Pawan KumarAbstract:Bac kground : A novel, patent-pending 20% Protodioscin-enriched extract has been developed in our laboratories from fenugreek seeds (Furosap ® ). We assessed the efficacy of Furosap ® in 50 male volunteers (age: 35-65 years) on free and total increased testosterone levels, sperm profile, mental alertness, cardiovascular health, mood, libido, and quality of life. Methods: Furosap ® (500 mg/day/subject) was administered to the 50 male volunteers over a period of 12 weeks in a one-arm, open-labelled study, to determine the efficacy on free and total testosterone levels, sperm profile and sperm morphology, libido and erectile dysfunction, mood and mental alertness and broad spectrum safety parameters. Institution Review Board approval was obtained for this study and the study was registered at the clinicaltrials.gov (NCT02702882). Results: A statistically significant increase in free testosterone levels were observed in these volunteers following supplementation of Furosap ® . Sperm morphology, sperm counts, mental alertness, mood, cardiovascular health, and libido performance were significantly improved. Extensive blood chemistry analyses revealed broad spectrum safety. No significant changes were observed in serum lipid function, cholesterol, triglyceride, HDL and LDL levels, and hemogram. Conclusions: Results confirmed that this Protodioscin-enriched extract from fenugreek seeds (Furosap) is safe and efficacious in boosting serum free testosterone levels, healthy sperm profile, mental alertness, cardiovascular health, and overall performance in male volunteers. Keywords: Standardized fenugreek seed extract; Protodioscin; Testosterone booster; Sperm profile; Mood alleviation; Safety
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Safety and Efficacy of Furosap, a novel Trigonella foenum-graecum seed extract, in Enhancing Testosterone Level and Improving Sperm Profile in Male Human Volunteers
The FASEB Journal, 2017Co-Authors: Manashi Bagchi, Anand Swaroop, Harry G. Preuss, Debasis BagchiAbstract:We have developed Furosap, an innovative, patent-pending 20% Protodioscin-enriched extract from fenugreek seeds, which effectively increased testosterone levels, and significantly improved sperm pr...
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A novel Protodioscin-enriched fenugreek seed extract (Trigonella foenum-graecum, family Fabaceae) improves free testosterone level and sperm profile in healthy volunteers
Food Science Publisher, 2017Co-Authors: Debasis Bagchi, Anand Swaroop, Manashi Bagchi, Harry G. Preuss, Anuj Maheshwari, Narsingh Verma, Kiran Tiwari, Pawan KumarAbstract:Background: A novel, patent-pending 20% Protodioscin-enriched extract has been developed in our laboratories from fenugreek seeds(Furosap®). We assessed the efficacy of Furosap®in 50 male volunteers (age: 35-65 years)on free and total increased testosterone levels, sperm profile, mental alertness, cardiovascular health, mood, libido,and quality of life. Methods: Furosap®(500 mg/day/subject)was administered to the 50 male volunteers over a period of 12 weeks in aone-arm, open-labelled study, to determine the efficacy on free and total testosterone levels, sperm profile andsperm morphology, libido and erectile dysfunction, mood and mental alertness and broad spectrum safety parameters. Institution Review Board approval was obtained for this study and the study was registered at the clinicaltrials.gov(NCT02702882).Results: A statistically significant increase in freetestosterone levels were observed in these volunteers following supplementation of Furosap®. Sperm morphology, sperm counts, mental alertness, mood, cardiovascular health,and libidoperformancewere significantly improved. Extensive blood chemistry analyses revealed broad spectrum safety. No significant changes were observed in serum lipid function, cholesterol, triglyceride, HDL and LDLlevels,andhemogram.Conclusions: Results confirmedthat this Protodioscin-enriched extract from fenugreek seeds (Furosap)is safe and efficacious in boosting serum free testosterone levels, healthy sperm profile, mental alertness, cardiovascular health,and overall performance in male volunteer
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Efficacy of Furosap, a novel Trigonella foenum-graecum seed extract, in Enhancing Healthy Testosterone Level and Improving Sperm Profile in Male Volunteers
The FASEB Journal, 2016Co-Authors: Anand Swaroop, Debasis Bagchi, Manashi Bagchi, Harry G. Preuss, Pawan KumarAbstract:Furosap, an innovative 20% Protodioscin-enriched extract derived from Trigonella foenum-graecum seeds, was developed in our laboratory, which effectively increased testosterone levels, sperm profil...
Depo Yang - One of the best experts on this subject based on the ideXlab platform.
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characterization of steroidal saponins in crude extract from dioscorea nipponica makino by liquid chromatography tandem multi stage mass spectrometry
Analytica Chimica Acta, 2007Co-Authors: Dongmei Wang, Depo Yang, Yao Tong, Jianping ChenAbstract:Abstract The liquid chromatography–electrospray ionization-tandem multi-stage mass spectrometry (LC–ESI-MS n ) method was developed for the analyses and characterization of steroidal saponins in plant extract from the rhizome of Dioscorea nipponica Makino. The HPLC experiments were performed by means of a reversed-phase C 18 column and a binary mobile phase system consisting of water and acetonitrile under gradient elution conditions. PseudoProtodioscin, methyl Protodioscin and dioscin were identified by comparing the retention times, UV spectra and the fragmentation properties of [ M − H] − ions with the authentic standards. Four groups of steroidal saponin isomers possessed the [ M − H] − ions at m / z 1063, 1045, 901 and 1047, respectively, were observed during the LC–ESI(−)-MS analysis, and three groups of them except the pair of isomers with the [ M − H] − ions at m / z 1047 could be differentiated by LC–ESI(−)-MS 3 . Furthermore, the ESI-MS n fragmentation behaviors of the [ M + Li] + ions of pseudoProtodioscin and methyl Protodioscin have been investigated, and the observed information helped the structural elucidation of the more abundant isomer with the [ M − H] − ion at m / z 1047. As the result, a special sugar sequence of the saccharide chains was observed that not glucose but rhamnose might be connected with the hydroxyl group at C-3 position of the steroidal aglycone.
