The Experts below are selected from a list of 534 Experts worldwide ranked by ideXlab platform
Cheming Teng - One of the best experts on this subject based on the ideXlab platform.
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Protopine a novel microtubule stabilizing agent causes mitotic arrest and apoptotic cell death in human hormone refractory prostate cancer cell lines
2012Co-Authors: Chunhan Chen, Chohwa Liao, Shiowlin Pan, Jihhwa Guh, Ya Ling Chang, Cheming TengAbstract:In this study, we investigated the anticancer effect of Protopine on human hormone-refractory prostate cancer (HRPC) cells. Protopine exhibited an anti-proliferative effect by induction of tubulin polymerization and mitotic arrest, which ultimately led to apoptotic cell death. The data suggest that Protopine increased the activity of cyclin-dependent kinase 1 (Cdk1)/cyclin B1 complex and that contributed to cell apoptosis by modulating mitochondria-mediated signaling pathways, such as Bcl-2 phosphorylation and Mcl-1 down-regulation. In conclusion, the data suggest that Protopine is a novel microtubule stabilizer with anticancer activity in HRPC cells through apoptotic pathway by modulating Cdk1 activity and Bcl-2 family of proteins.
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antihaemostatic and antithrombotic effect of some antiplatelet agents isolated from chinese herbs
2011Co-Authors: Cheming Teng, Jihpyang Wang, Chunnan N Lin, Chienchih C Chen, Turfu HuangAbstract:— Five antiplatelet agents have been isolated from Chinese herbs. Apigenin and magnolol are inhibitors of thromboxane synthesis, while osthole, Protopine and norathyriol are inhibitors of phosphoinositide breakdown. Thirty min after intraperitoneal (i.p.) administration of these drugs, tail bleeding time of mice was prolonged markedly in a dose-dependent manner by norathyriol, Protopine, osthole and magnolol, but not by apigenin. However, the antiplatelet agents (up to 200 mg kg−1, i.p.) could not prevent acute thromboembolic death in mice. In endotoxin-induced experimental disseminated intravascular coagulation in rats, norathyriol (50–100 mg kg−1, i.p.) prevented the decrease in platelet counts and fibrinogen, and the prolongation of plasma prothrombin time. Norathyriol (100 mg kg−1, i.p.) also suppressed ex-vivo platelet aggregation induced by collagen and ADP in rat plasma.
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Protopine a novel microtubule stabilizing agent caused mitotic arrest and subsequent apoptosis in hormone refractory prostate cancer cells
2007Co-Authors: Chunhan Chen, Chohwa Liao, Shiowlin Pan, Jihhwa Guh, Cheming TengAbstract:B42 Prostate cancer is the second leading cause of cancer death in men. Because microtubule-stablizing agents, such as docetaxel which has been approved by the Food and Drug Administration for advanced prostate cancer, might have the therapeutic potential, we examined the anticancer effect of Protopine in metastatic hormone-refractory prostate cancer PC-3 cells. Protopine is an isoquinoline alkaloid which is common in the plants of Papaveraceae , Fumariaceae and other families. Protopine exhibited anti-proliferative effect on PC-3 cells in a concentration-dependent manner with a GI 50 of 13.02 ± 1.70 μM as evaluated by sulforhodamine B assay. FACScan flow cytometric analysis revealed that Protopine caused cell cycle accumulation at G2/M phase and subsequent at subG1 phase. Meanwhile, Protopine activated cyclin-dependent kinase 1 (CDK1) through down-regulation of Tyr15 phosphorylation and up-regulation of Thr161 phosphorylation as well as induction of cyclin B1. By the immunofluorescence microscopy and tubulin polymerization assay, Protopine concentration-dependently promoted tubulin polymerization. Furthermore, Protopine induced massive apoptotic cell death as detected by TdT-mediated dUTP Nick-End Labeling (TUNEL) assay and oligonucleosomal DNA fragmentation. Protopine increased Bcl-2 and Mcl-1 phosphorylation and eventually caspase-3 activation following PARP cleavage. Overall, this is the first report to demonstrate that Protopine stabilized microtubule organization leading to mitotic arrest and subsequent apoptosis in metastatic hormone-refractory prostate cancer PC-3 cell line.
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ca2 channel blockade in rat thoracic aorta by Protopine isolated from corydalis tubers
1992Co-Authors: Turfu Huang, Cheming TengAbstract:The pharmacological properties and mechanism of the action of Protopine on isolated rat thoracic aorta were examined. It inhibited norepinephrine (NE, 3 microM)-induced tonic contraction in rat thoracic aorta in a concentration-dependent manner (25-100 micrograms/ml). The phasic contraction caused by NE was inhibited only by a high concentration of Protopine (100 micrograms/ml). At the plateau of NE-induced tonic contraction, the addition of Protopine also caused relaxation. This relaxing effect of Protopine was not antagonized by indomethacin (20 microM) or methylene blue (50 microM), and it still existed in denuded rat aorta or in the presence of nifedipine (2-100 microM). Protopine also inhibited high potassium (60 mM)-induced, calcium-dependent (0.03-3 mM) contraction of rat aorta in a concentration-dependent manner. Neither cAMP nor cGMP level was changed by Protopine. Both the formation of inositol monophosphate caused by NE and the phasic contraction induced by caffeine were also not affected by Protopine. 45Ca2+ influx caused by either NE or K+ was inhibited by Protopine concentration-dependently. It is concluded that Protopine relaxed the rat thoracic aorta mainly by suppressing the Ca2+ influx through both voltage- and receptor-operated calcium channels.
Shouzhuo Yao - One of the best experts on this subject based on the ideXlab platform.
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rapid determination of Protopine allocryptopine sanguinarine and chelerythrine in fruits of macleaya cordata by microwave assisted solvent extraction and hplc esi ms
2006Co-Authors: Xubiao Luo, Bo Chen, Shouzhuo YaoAbstract:An isocratic high-performance liquid chromatographic method coupled with electrospray mass spectrometry was developed to determine Protopine, allocryptopine, sanguinarine and chelerythrine in fruits of Macleaya cordata. The sample was extracted with hydrochloric acid aqueous solution using microwave-assisted extraction method. The extracts were separated on a C8 reversed-phase HPLC column with acetonitrile:acetate buffer as mobile phase, and full elution of all analytes was realized isocratically within 10 min. The abundance of pseudomolecule ions was recorded using selected ion recording at m/z 354.4, 370.1, 332.5, 348.5 and 338.5 for Protopine, allocryptopine, sanguinarine, chelerythrine and the internal standard, jatrorrhizine, respectively. Internal standard curves were used for the quantification of Protopine, allocryptopine, sanguinarine and chelerythrine, which showed a linear range of 0.745-74.5, 0.610-61.0, 0.525-105 and 0.375-75 microg/mL, respectively, with correlation coefficients of 0.9995, 0.9992, 0.9993 and 0.9989, and limits of detection of 3.73, 3.05, 1.60 and 1.11 ng/mL, respectively.
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Rapid determination of Protopine, allocryptopine, sanguinarine and chelerythrine in fruits of Macleaya cordata by microwave-assisted solvent extraction and HPLC-ESI/MS.
2006Co-Authors: Xubiao Luo, Bo Chen, Shouzhuo YaoAbstract:An isocratic high-performance liquid chromatographic method coupled with electrospray mass spectrometry was developed to determine Protopine, allocryptopine, sanguinarine and chelerythrine in fruits of Macleaya cordata. The sample was extracted with hydrochloric acid aqueous solution using microwave-assisted extraction method. The extracts were separated on a C8 reversed-phase HPLC column with acetonitrile:acetate buffer as mobile phase, and full elution of all analytes was realized isocratically within 10 min. The abundance of pseudomolecule ions was recorded using selected ion recording at m/z 354.4, 370.1, 332.5, 348.5 and 338.5 for Protopine, allocryptopine, sanguinarine, chelerythrine and the internal standard, jatrorrhizine, respectively. Internal standard curves were used for the quantification of Protopine, allocryptopine, sanguinarine and chelerythrine, which showed a linear range of 0.745–74.5, 0.610–61.0, 0.525–105 and 0.375–75 µg/mL, respectively, with correlation coefficients of 0.9995, 0.9992, 0.9993 and 0.9989, and limits of detection of 3.73, 3.05, 1.60 and 1.11 ng/mL, respectively. Copyright © 2006 John Wiley & Sons, Ltd.
Chunhan Chen - One of the best experts on this subject based on the ideXlab platform.
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Protopine a novel microtubule stabilizing agent causes mitotic arrest and apoptotic cell death in human hormone refractory prostate cancer cell lines
2012Co-Authors: Chunhan Chen, Chohwa Liao, Shiowlin Pan, Jihhwa Guh, Ya Ling Chang, Cheming TengAbstract:In this study, we investigated the anticancer effect of Protopine on human hormone-refractory prostate cancer (HRPC) cells. Protopine exhibited an anti-proliferative effect by induction of tubulin polymerization and mitotic arrest, which ultimately led to apoptotic cell death. The data suggest that Protopine increased the activity of cyclin-dependent kinase 1 (Cdk1)/cyclin B1 complex and that contributed to cell apoptosis by modulating mitochondria-mediated signaling pathways, such as Bcl-2 phosphorylation and Mcl-1 down-regulation. In conclusion, the data suggest that Protopine is a novel microtubule stabilizer with anticancer activity in HRPC cells through apoptotic pathway by modulating Cdk1 activity and Bcl-2 family of proteins.
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Protopine a novel microtubule stabilizing agent caused mitotic arrest and subsequent apoptosis in hormone refractory prostate cancer cells
2007Co-Authors: Chunhan Chen, Chohwa Liao, Shiowlin Pan, Jihhwa Guh, Cheming TengAbstract:B42 Prostate cancer is the second leading cause of cancer death in men. Because microtubule-stablizing agents, such as docetaxel which has been approved by the Food and Drug Administration for advanced prostate cancer, might have the therapeutic potential, we examined the anticancer effect of Protopine in metastatic hormone-refractory prostate cancer PC-3 cells. Protopine is an isoquinoline alkaloid which is common in the plants of Papaveraceae , Fumariaceae and other families. Protopine exhibited anti-proliferative effect on PC-3 cells in a concentration-dependent manner with a GI 50 of 13.02 ± 1.70 μM as evaluated by sulforhodamine B assay. FACScan flow cytometric analysis revealed that Protopine caused cell cycle accumulation at G2/M phase and subsequent at subG1 phase. Meanwhile, Protopine activated cyclin-dependent kinase 1 (CDK1) through down-regulation of Tyr15 phosphorylation and up-regulation of Thr161 phosphorylation as well as induction of cyclin B1. By the immunofluorescence microscopy and tubulin polymerization assay, Protopine concentration-dependently promoted tubulin polymerization. Furthermore, Protopine induced massive apoptotic cell death as detected by TdT-mediated dUTP Nick-End Labeling (TUNEL) assay and oligonucleosomal DNA fragmentation. Protopine increased Bcl-2 and Mcl-1 phosphorylation and eventually caspase-3 activation following PARP cleavage. Overall, this is the first report to demonstrate that Protopine stabilized microtubule organization leading to mitotic arrest and subsequent apoptosis in metastatic hormone-refractory prostate cancer PC-3 cell line.
Xubiao Luo - One of the best experts on this subject based on the ideXlab platform.
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rapid determination of Protopine allocryptopine sanguinarine and chelerythrine in fruits of macleaya cordata by microwave assisted solvent extraction and hplc esi ms
2006Co-Authors: Xubiao Luo, Bo Chen, Shouzhuo YaoAbstract:An isocratic high-performance liquid chromatographic method coupled with electrospray mass spectrometry was developed to determine Protopine, allocryptopine, sanguinarine and chelerythrine in fruits of Macleaya cordata. The sample was extracted with hydrochloric acid aqueous solution using microwave-assisted extraction method. The extracts were separated on a C8 reversed-phase HPLC column with acetonitrile:acetate buffer as mobile phase, and full elution of all analytes was realized isocratically within 10 min. The abundance of pseudomolecule ions was recorded using selected ion recording at m/z 354.4, 370.1, 332.5, 348.5 and 338.5 for Protopine, allocryptopine, sanguinarine, chelerythrine and the internal standard, jatrorrhizine, respectively. Internal standard curves were used for the quantification of Protopine, allocryptopine, sanguinarine and chelerythrine, which showed a linear range of 0.745-74.5, 0.610-61.0, 0.525-105 and 0.375-75 microg/mL, respectively, with correlation coefficients of 0.9995, 0.9992, 0.9993 and 0.9989, and limits of detection of 3.73, 3.05, 1.60 and 1.11 ng/mL, respectively.
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Rapid determination of Protopine, allocryptopine, sanguinarine and chelerythrine in fruits of Macleaya cordata by microwave-assisted solvent extraction and HPLC-ESI/MS.
2006Co-Authors: Xubiao Luo, Bo Chen, Shouzhuo YaoAbstract:An isocratic high-performance liquid chromatographic method coupled with electrospray mass spectrometry was developed to determine Protopine, allocryptopine, sanguinarine and chelerythrine in fruits of Macleaya cordata. The sample was extracted with hydrochloric acid aqueous solution using microwave-assisted extraction method. The extracts were separated on a C8 reversed-phase HPLC column with acetonitrile:acetate buffer as mobile phase, and full elution of all analytes was realized isocratically within 10 min. The abundance of pseudomolecule ions was recorded using selected ion recording at m/z 354.4, 370.1, 332.5, 348.5 and 338.5 for Protopine, allocryptopine, sanguinarine, chelerythrine and the internal standard, jatrorrhizine, respectively. Internal standard curves were used for the quantification of Protopine, allocryptopine, sanguinarine and chelerythrine, which showed a linear range of 0.745–74.5, 0.610–61.0, 0.525–105 and 0.375–75 µg/mL, respectively, with correlation coefficients of 0.9995, 0.9992, 0.9993 and 0.9989, and limits of detection of 3.73, 3.05, 1.60 and 1.11 ng/mL, respectively. Copyright © 2006 John Wiley & Sons, Ltd.
Chohwa Liao - One of the best experts on this subject based on the ideXlab platform.
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Protopine a novel microtubule stabilizing agent causes mitotic arrest and apoptotic cell death in human hormone refractory prostate cancer cell lines
2012Co-Authors: Chunhan Chen, Chohwa Liao, Shiowlin Pan, Jihhwa Guh, Ya Ling Chang, Cheming TengAbstract:In this study, we investigated the anticancer effect of Protopine on human hormone-refractory prostate cancer (HRPC) cells. Protopine exhibited an anti-proliferative effect by induction of tubulin polymerization and mitotic arrest, which ultimately led to apoptotic cell death. The data suggest that Protopine increased the activity of cyclin-dependent kinase 1 (Cdk1)/cyclin B1 complex and that contributed to cell apoptosis by modulating mitochondria-mediated signaling pathways, such as Bcl-2 phosphorylation and Mcl-1 down-regulation. In conclusion, the data suggest that Protopine is a novel microtubule stabilizer with anticancer activity in HRPC cells through apoptotic pathway by modulating Cdk1 activity and Bcl-2 family of proteins.
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Protopine a novel microtubule stabilizing agent caused mitotic arrest and subsequent apoptosis in hormone refractory prostate cancer cells
2007Co-Authors: Chunhan Chen, Chohwa Liao, Shiowlin Pan, Jihhwa Guh, Cheming TengAbstract:B42 Prostate cancer is the second leading cause of cancer death in men. Because microtubule-stablizing agents, such as docetaxel which has been approved by the Food and Drug Administration for advanced prostate cancer, might have the therapeutic potential, we examined the anticancer effect of Protopine in metastatic hormone-refractory prostate cancer PC-3 cells. Protopine is an isoquinoline alkaloid which is common in the plants of Papaveraceae , Fumariaceae and other families. Protopine exhibited anti-proliferative effect on PC-3 cells in a concentration-dependent manner with a GI 50 of 13.02 ± 1.70 μM as evaluated by sulforhodamine B assay. FACScan flow cytometric analysis revealed that Protopine caused cell cycle accumulation at G2/M phase and subsequent at subG1 phase. Meanwhile, Protopine activated cyclin-dependent kinase 1 (CDK1) through down-regulation of Tyr15 phosphorylation and up-regulation of Thr161 phosphorylation as well as induction of cyclin B1. By the immunofluorescence microscopy and tubulin polymerization assay, Protopine concentration-dependently promoted tubulin polymerization. Furthermore, Protopine induced massive apoptotic cell death as detected by TdT-mediated dUTP Nick-End Labeling (TUNEL) assay and oligonucleosomal DNA fragmentation. Protopine increased Bcl-2 and Mcl-1 phosphorylation and eventually caspase-3 activation following PARP cleavage. Overall, this is the first report to demonstrate that Protopine stabilized microtubule organization leading to mitotic arrest and subsequent apoptosis in metastatic hormone-refractory prostate cancer PC-3 cell line.
