The Experts below are selected from a list of 219 Experts worldwide ranked by ideXlab platform
J. B. Power - One of the best experts on this subject based on the ideXlab platform.
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Encyclopedia of Cell Technology - Protoplast Fusion for the Generation of Unique Plants
Encyclopedia of Cell Technology, 2003Co-Authors: Michael R. Davey, Kenneth C. Lowe, J. B. Power, Paul AnthonyAbstract:Introduction Why Fuse Isolated Plant Protoplasts? Procedures for Protoplast Fusion Chemical Fusion of Protoplasts Electrical Fusion of Protoplasts Products of Protoplast Fusion and the Selection of Somatic Hybrid Tissues and Plants Somatic Hybrid Tissues and Plants Homokaryons and Heterokaryons Procedures for Selecting Hybrid Cells and Plants Characterization of Somatic Hybrid Plants Generation of Asymmetrical Hybrids and Cybrids by Protoplast Fusion Gametosomatic Hybridization Transfer of Unique Traits by Protoplast Fusion Concluding Remarks Bibliography Keywords: hybrids; genetic engineering; gene transfer
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[29] Plant Protoplast Fusion and somatic hybridization
Methods in Enzymology, 1993Co-Authors: P. T. Lynch, M. R. Davey, J. B. PowerAbstract:Publisher Summary This chapter discusses the plant Protoplast Fusion and somatic hybridization. Somatic hybridization involves four discrete, yet interrelated, stages: (1) Protoplast isolation and culture with efficient plant regeneration in at least one of the Fusion partners, (2) induced Protoplast Fusion, preferably at high frequency, without loss of viability, (3) the development of a selection strategy incorporating somatic hybrid plant regeneration, and (4) the confirmation of hybridity or cybridity. Such confirmation utilizes cytological and morphological markers, and a range of biochemical-based techniques. Although conventional methods of plant breeding will continue to play a major role in crop improvement, somatic hybridization will offer a unique opportunity for achieving gene flow in plants, particularly for the transfer of reproductively isolated multigenic traits. The application of Protoplast Fusion to plant breeding depends on continued extension of the range of crop plants that can be regenerated from Protoplasts, together with refinement of the procedures for the selection of somatic hybrid tissues and plants.
Byong Kak Kim - One of the best experts on this subject based on the ideXlab platform.
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Ultrastructural Process of Protoplast Fusion Between Lentinula edodes and Coriolus versicolor
Mycobiology, 2001Co-Authors: Chaekyun Kim, Byong Kak KimAbstract:Protoplast Fusion is a useful technique for establishing fungal hybrids to overcome the natural barriers. The ultrastructure of Protoplast and its Fusion process were observed using a scanning elec...
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Protoplast Fusion betweenLentinula edodes andCoriolus versicolor.
Archives of pharmacal research, 1997Co-Authors: Chaekyun Kim, Eung Chil Choi, Byong Kak KimAbstract:Protoplast Fusion between isoleucine-, arginine- and thymidine-requiring auxotroph (lle, Arg, Thy) ofLentinula edodes and arginine-requiring auxotroph (Arg) ofCoriolus versicolor has been achieved using 30% polyethylene glycol (M.W. 4000) in 10 mMCaCl2-glycine solution (pH 8.0). Fusion hybrids were selected in the 0.6 M sucrose supplemented minimal media on the basis of nutritional complementation with Fusion frequency of 7.4×10−6. The hybrids included both parental and non-parental types in colony morphology, growth rate and isozyme patterns. We succeeded inter-order Protoplast Fusion between the auxotrophs ofLentinula edodes and Coriolus versicolor overcoming the natural barriers of incompatibility. We examined the characteristics of the hybrids and clarified the Fusion process using electron microscopy.
Barbel Foroughiwehr - One of the best experts on this subject based on the ideXlab platform.
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Protoplast Fusion in banana musa spp comparison of chemical peg polyethylene glycol and electrical procedure
Plant Cell Tissue and Organ Culture, 2005Co-Authors: Akym Assani, Djamila Chabane, Robert Haicour, Frederic Bakry, Gerhard Wenzel, Barbel ForoughiwehrAbstract:Optimization of Protoplast Fusion parameters is a prerequisite for the establishment of somatic Fusion technology for banana breeding. In the present investigations, we compared the most frequently used Fusion methods: the electroFusion technique and chemical procedure (polyethylene glycol). With regard to frequency of binary Fusion, Protoplast Fusion with the fusogen polyethylene glycol was best. Conversely, electric Fusion was found to be better with respect to mitotic activities, somatic embryogenesis and plantlet regeneration rate.
Chaekyun Kim - One of the best experts on this subject based on the ideXlab platform.
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Ultrastructural Process of Protoplast Fusion Between Lentinula edodes and Coriolus versicolor
Mycobiology, 2001Co-Authors: Chaekyun Kim, Byong Kak KimAbstract:Protoplast Fusion is a useful technique for establishing fungal hybrids to overcome the natural barriers. The ultrastructure of Protoplast and its Fusion process were observed using a scanning elec...
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Protoplast Fusion betweenLentinula edodes andCoriolus versicolor.
Archives of pharmacal research, 1997Co-Authors: Chaekyun Kim, Eung Chil Choi, Byong Kak KimAbstract:Protoplast Fusion between isoleucine-, arginine- and thymidine-requiring auxotroph (lle, Arg, Thy) ofLentinula edodes and arginine-requiring auxotroph (Arg) ofCoriolus versicolor has been achieved using 30% polyethylene glycol (M.W. 4000) in 10 mMCaCl2-glycine solution (pH 8.0). Fusion hybrids were selected in the 0.6 M sucrose supplemented minimal media on the basis of nutritional complementation with Fusion frequency of 7.4×10−6. The hybrids included both parental and non-parental types in colony morphology, growth rate and isozyme patterns. We succeeded inter-order Protoplast Fusion between the auxotrophs ofLentinula edodes and Coriolus versicolor overcoming the natural barriers of incompatibility. We examined the characteristics of the hybrids and clarified the Fusion process using electron microscopy.
P. T. Lynch - One of the best experts on this subject based on the ideXlab platform.
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[29] Plant Protoplast Fusion and somatic hybridization
Methods in Enzymology, 1993Co-Authors: P. T. Lynch, M. R. Davey, J. B. PowerAbstract:Publisher Summary This chapter discusses the plant Protoplast Fusion and somatic hybridization. Somatic hybridization involves four discrete, yet interrelated, stages: (1) Protoplast isolation and culture with efficient plant regeneration in at least one of the Fusion partners, (2) induced Protoplast Fusion, preferably at high frequency, without loss of viability, (3) the development of a selection strategy incorporating somatic hybrid plant regeneration, and (4) the confirmation of hybridity or cybridity. Such confirmation utilizes cytological and morphological markers, and a range of biochemical-based techniques. Although conventional methods of plant breeding will continue to play a major role in crop improvement, somatic hybridization will offer a unique opportunity for achieving gene flow in plants, particularly for the transfer of reproductively isolated multigenic traits. The application of Protoplast Fusion to plant breeding depends on continued extension of the range of crop plants that can be regenerated from Protoplasts, together with refinement of the procedures for the selection of somatic hybrid tissues and plants.
