The Experts below are selected from a list of 2883 Experts worldwide ranked by ideXlab platform
Ed X. Wu - One of the best experts on this subject based on the ideXlab platform.
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Preliminary in vitro study of ultrasound sonoporation cell labeling with superparamagnetic iron oxide particles for MRI cell tracking
2008 30th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 2008Co-Authors: Runyang Mo, Gongzheng Wang, Yu Wang, Ed X. WuAbstract:Vibration caused by ultrasonic waves can change the structure of cell membrane and enhance its permeation. In the last decade, a new ultrasound-aided method, sonoporation, has been proposed and utilized to transmit target molecules (such as drugs and DNA) into cells for therapy. The objective of this study was to investigate the method of loading nanometer-sized superparamagnetic iron oxide particles into Sarcoma 180 cells by sonoporation without chemical agents. The SPIO nanoparticles were prepared in our laboratory by means of classical coprecipitation and the formation of Fe3O4 crystal in SPIO nanoparticles was confirmed by x-ray diffraction analysis with its other characteristics assessed by magnetic hysteresis loops and size distribution. Cell labeling with SPIOs using sonoporation was successfully demonstrated in vitro for sarcoma180 cell suspensions from ICR mice. The labeling efficiency and viability were evaluated by Prussian Blue Staining. Such sonoporation technique can be employed for rapid labeling of various cells for MRI visualization of their spatiotemporal activities in vivo upon transplantation.
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Magnetic resonance imaging of migrating neuronal precursors in normal and hypoxic-ischemic neonatal rat brains by intraventricular MPIO labeling
2008 30th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 2008Co-Authors: Jian Yang, Ed X. WuAbstract:In this study, 10-day-old normal rats (n=6) and hypoxic-ischemic (H-I) neonatal rats (n=6) were injected with the micronsized iron oxide particles (MPIOs) into the anterior lateral ventricle. 2D and 3D high-spatial resolution MRI were performed with a 7T animal scanner 1 day before the MPIOs injection and hour 3, day 3, day 7 and day 14 after the MPIOs injection. Intraperitoneal injections of 5-bromo-2′-deoxyuridine (BrdU) were used to label newly produced cells, and were given thrice daily for 2 days before sacrifice. Immunohistochemistry and Prussian Blue Staining indicated that iron particles were inside the nestin+ and BrdU+ neural progenitor cells (NPCs), glial-fibrillary-acidic-protein-positive (GFAP+) astrocytes-like progenitor cells, and neuronal-nuclei-positive (NeuN+) mature neurons. Here we demonstrate that, in normal neonatal rat brain, the migrating pathway of the endogenous NPCs with MPIO is mainly along the rostral migratory stream to the olfactory bulb. In H-I neonatal rat brain, the migration of endogenous NPCs with MPIO is mainly towards the ischemic regions. Therefore, in vivo magnetic cell labeling of endogenous NPCs with MPIO and subsequently non-invasive, serial MRI monitoring should open up a new approach to probe into the mechanism of cell migration in the developmental brain under physiological and pathologic conditions.
Xian Long Zhang - One of the best experts on this subject based on the ideXlab platform.
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Biocompatibility of chitosan-coated iron oxide nanoparticles with osteoblast cells
International Journal of Nanomedicine, 2012Co-Authors: Si Feng Shi, Ya Ping Zhao, Jing Fu Jia, Xiao Kui Guo, De Sheng Chen, Yong Yuan Guo, Tao Cheng, Xian Long ZhangAbstract:BACKGROUND: Bone disorders (including osteoporosis, loosening of a prosthesis, and bone infections) are of great concern to the medical community and are difficult to cure. Therapies are available to treat such diseases, but all have drawbacks and are not specifically targeted to the site of disease. Chitosan is widely used in the biomedical community, including for orthopedic applications. The aim of the present study was to coat chitosan onto iron oxide nanoparticles and to determine its effect on the proliferation and differentiation of osteoblasts.\n\nMETHODS: Nanoparticles were characterized using transmission electron microscopy, dynamic light scattering, x-ray diffraction, zeta potential, and vibrating sample magnetometry. Uptake of nanoparticles by osteoblasts was studied by transmission electron microscopy and Prussian Blue Staining. Viability and proliferation of osteoblasts were measured in the presence of uncoated iron oxide magnetic nanoparticles or those coated with chitosan. Lactate dehydrogenase, alkaline phosphatase, total protein synthesis, and extracellular calcium deposition was studied in the presence of the nanoparticles.\n\nRESULTS: Chitosan-coated iron oxide nanoparticles enhanced osteoblast proliferation, decreased cell membrane damage, and promoted cell differentiation, as indicated by an increase in alkaline phosphatase and extracellular calcium deposition. Chitosan-coated iron oxide nanoparticles showed good compatibility with osteoblasts.\n\nCONCLUSION: Further research is necessary to optimize magnetic nanoparticles for the treatment of bone disease.
Hyuck Jae Choi - One of the best experts on this subject based on the ideXlab platform.
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In Vivo Dual-Modality Terahertz/Magnetic Resonance Imaging Using Superparamagnetic Iron Oxide Nanoparticles as a Dual Contrast Agent
IEEE Transactions on Terahertz Science and Technology, 2012Co-Authors: Jae Yeon Park, Hyuck Jae ChoiAbstract:Molecular imaging is one of the most promising tools for diagnosis of cancer. We assessed whether commercially available superparamagnetic iron oxide nanoparticles (SPIO; Feridex®) could be utilized as a dual modality contrast agent for terahertz (THz) imaging as well as magnetic resonance (MR) imaging. Feridex particles were transfected into SKOV3 cancer cells, at concentrations of 0, 0.35, 0.70, and 1.38 mM, and the magnetic and optical properties of the particles were examined by MR and THz reflection imaging. Mice were inoculated with Feridex-labeled SKOV3 cells, and in vivo MR and THz images were taken 1, 3, 7, and 14 days after inoculation. THz images and T2*-weighted MR images of Feridex-labeled SKOV3 tumors showed similar patterns; the signal intensities of both image sets increased with Feridex concentration. The signal intensity of in vivo MR and THz images from mice decreased over time. H&E and Prussian Blue Staining results correlated with imaging data. Dual-modality molecular MR and THz imaging of Feridex-labeled cells may be used to identify cancer cells both in vivo and in vitro. Such a noninvasive multimodal imaging method may be valuable in future cellular and molecular studies.
Si Feng Shi - One of the best experts on this subject based on the ideXlab platform.
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Biocompatibility of chitosan-coated iron oxide nanoparticles with osteoblast cells
International Journal of Nanomedicine, 2012Co-Authors: Si Feng Shi, Ya Ping Zhao, Jing Fu Jia, Xiao Kui Guo, De Sheng Chen, Yong Yuan Guo, Tao Cheng, Xian Long ZhangAbstract:BACKGROUND: Bone disorders (including osteoporosis, loosening of a prosthesis, and bone infections) are of great concern to the medical community and are difficult to cure. Therapies are available to treat such diseases, but all have drawbacks and are not specifically targeted to the site of disease. Chitosan is widely used in the biomedical community, including for orthopedic applications. The aim of the present study was to coat chitosan onto iron oxide nanoparticles and to determine its effect on the proliferation and differentiation of osteoblasts.\n\nMETHODS: Nanoparticles were characterized using transmission electron microscopy, dynamic light scattering, x-ray diffraction, zeta potential, and vibrating sample magnetometry. Uptake of nanoparticles by osteoblasts was studied by transmission electron microscopy and Prussian Blue Staining. Viability and proliferation of osteoblasts were measured in the presence of uncoated iron oxide magnetic nanoparticles or those coated with chitosan. Lactate dehydrogenase, alkaline phosphatase, total protein synthesis, and extracellular calcium deposition was studied in the presence of the nanoparticles.\n\nRESULTS: Chitosan-coated iron oxide nanoparticles enhanced osteoblast proliferation, decreased cell membrane damage, and promoted cell differentiation, as indicated by an increase in alkaline phosphatase and extracellular calcium deposition. Chitosan-coated iron oxide nanoparticles showed good compatibility with osteoblasts.\n\nCONCLUSION: Further research is necessary to optimize magnetic nanoparticles for the treatment of bone disease.
Yu Wang - One of the best experts on this subject based on the ideXlab platform.
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In vivo magnetic resonance imaging tracking of transplanted adipose-derived stem cells labeled with superparamagnetic iron oxide in rat hearts
Acta Academiae Medicinae Sinicae, 2009Co-Authors: Yu Wang, Wang Gy, Li Xh, Yun Li, Liang ChAbstract:Objective To investigate the feasibility of in vivo magnetic resonance imaging (MRI) tracking of transplanted adipose-derived stem cells (ADSCs) labeled with superparamagnetic iron oxide (SPIO) in rat heart.Methods ADSCs were labeled with poly-L-lysine (PLL)-SPIO complexes. Intracellular iron uptake was identified by Prussian Blue stain and transmission electromicroscopy. Trypan Blue Staining was used to test the viability of the labeled cells. In vitro MRI of labeled cells was performed. SPIO-labeled ADSCs were transplanted into normal rat hearts and were in vivo imaged with MRI. Image findings on MRI were correlated with histological findings of the rat hearts. Results The labeling efficacy of ADSCs with PLL-SPIO was nearly 100%. Light microscopy revealed the SPIO particles were located in the cytoplasm of the ADSCs by Prussian Blue Staining. Transmission electromicroscopy revealed that the SPIO particles were located in the endosomes in the cytoplasm. There was no significantly deference in viability between labeled and unlabeled groups demonstrated by Trypan Blue test (P0.05). MRI showed signal loss in gel mixed with labeled cells as compared with the unlabeled cells group and blank group. Signal void on rat hearts were demonstrated on MRI and were well correlated with histological findings where Prussian-Blue-stain positive cells presented. Conclusion MRI can be used to in vivo track the transplanted ADSCs labeled with SPIO into rat hearts and facilitate to understand the conditions of the labeled cells in the transplanted areas.
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Preliminary in vitro study of ultrasound sonoporation cell labeling with superparamagnetic iron oxide particles for MRI cell tracking
2008 30th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 2008Co-Authors: Runyang Mo, Gongzheng Wang, Yu Wang, Ed X. WuAbstract:Vibration caused by ultrasonic waves can change the structure of cell membrane and enhance its permeation. In the last decade, a new ultrasound-aided method, sonoporation, has been proposed and utilized to transmit target molecules (such as drugs and DNA) into cells for therapy. The objective of this study was to investigate the method of loading nanometer-sized superparamagnetic iron oxide particles into Sarcoma 180 cells by sonoporation without chemical agents. The SPIO nanoparticles were prepared in our laboratory by means of classical coprecipitation and the formation of Fe3O4 crystal in SPIO nanoparticles was confirmed by x-ray diffraction analysis with its other characteristics assessed by magnetic hysteresis loops and size distribution. Cell labeling with SPIOs using sonoporation was successfully demonstrated in vitro for sarcoma180 cell suspensions from ICR mice. The labeling efficiency and viability were evaluated by Prussian Blue Staining. Such sonoporation technique can be employed for rapid labeling of various cells for MRI visualization of their spatiotemporal activities in vivo upon transplantation.
