The Experts below are selected from a list of 165 Experts worldwide ranked by ideXlab platform
Anita Huovilainen - One of the best experts on this subject based on the ideXlab platform.
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recent isolates of parapoxVirus of finnish reindeer rangifer tarandus tarandus are closely related to bovine Pseudocowpox Virus
Journal of General Virology, 2004Co-Authors: Maria K Tikkanen, Jarno Tuimala, Varpu Hirvelakoski, Colin Mcinnes, Mathias Büttner, Erkki Neuvonen, Anita HuovilainenAbstract:Cases of papular stomatitis in Finnish reindeer have been reported for many years. The causative agent was thought to be Orf Virus (ORFV), one of the Parapoxviridae, although this assumption was based mainly on clinical symptoms, pathology and electron microscopy. Here sequence analyses of the viral DNA isolated from a recent outbreak of disease in 1999–2000 are presented in comparison to that isolated from earlier outbreaks in 1992–1994. The results show that the Virus isolated from the 1999–2000 outbreak is most closely related to Pseudocowpox Virus, whereas those from previous years grouped with ORFV. The present study describes a method for genetic characterization and classification of parapoxViruses (PPVs) and provides for the first time an extended phylogenetic analysis of PPVs isolated from Finland, established members of the genus ParapoxVirus and selected members of the subfamily Chordopoxvirinae.
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Short Communication Recent isolates of parapoxVirus of Finnish reindeer (Rangifer tarandus tarandus) are closely related to bovine Pseudocowpox Virus
2004Co-Authors: Maria K Tikkanen, Jarno Tuimala, Colin Mcinnes, Erkki Neuvonen, Anita HuovilainenAbstract:Cases of papular stomatitis in Finnish reindeer have been reported for many years. The causative agent was thought to be Orf Virus (ORFV), one of the Parapoxviridae, although this assumption was based mainly on clinical symptoms, pathology and electron microscopy. Here sequence analyses of the viral DNA isolated from a recent outbreak of disease in 1999–2000 are presented in comparison to that isolated from earlier outbreaks in 1992–1994. The results show that the Virus isolated from the 1999–2000 outbreak is most closely related to Pseudocowpox Virus, whereas those from previous years grouped with ORFV. The present study describes a method for genetic characterization and classification of parapoxViruses (PPVs) and provides for the first time an extended phylogenetic analysis of PPVs isolated from Finland, established members of the genus ParapoxVirus and selected members of the subfamily Chordopoxvirinae.
Colin Mcinnes - One of the best experts on this subject based on the ideXlab platform.
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Conservation and variation of the parapoxVirus GM-CSF-inhibitory factor (GIF) proteins
Journal of General Virology, 2009Co-Authors: David Deane, Norihito Ueda, Lyn M. Wise, Stephen B. Fleming, Ann R. Wood, Ann Percival, C. Jepson, Neil F. Inglis, Colin McinnesAbstract:The GIF protein of orf Virus (ORFV) binds and inhibits the ovine cytokines granulocyte–macrophage colony-stimulating factor (GM-CSF) and interleukin-2 (IL-2). An equivalent protein has so far not been found in any of the other poxVirus genera and we therefore investigated whether it was conserved in the parapoxViruses. The corresponding genes from both the bovine-specific Pseudocowpox Virus (PCPV) and bovine papular stomatitis Virus (BPSV) were cloned and sequenced. The predicted amino acid sequences of the PCPV and BPSV proteins shared 88 and 37 % identity, respectively, with the ORFV protein. Both retained the six cysteine residues and the WSXWS-like motif that are required for biological activity of the ORFV protein. However, an analysis of the biological activity of the two recombinant proteins revealed that, whilst the PCPV GIF protein bound to both ovine and bovine GM-CSF and IL-2 with very similar binding affinities to the ORFV GIF protein, no GM-CSF- or IL-2-binding activity was found for the BPSV protein.
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recent isolates of parapoxVirus of finnish reindeer rangifer tarandus tarandus are closely related to bovine Pseudocowpox Virus
Journal of General Virology, 2004Co-Authors: Maria K Tikkanen, Jarno Tuimala, Varpu Hirvelakoski, Colin Mcinnes, Mathias Büttner, Erkki Neuvonen, Anita HuovilainenAbstract:Cases of papular stomatitis in Finnish reindeer have been reported for many years. The causative agent was thought to be Orf Virus (ORFV), one of the Parapoxviridae, although this assumption was based mainly on clinical symptoms, pathology and electron microscopy. Here sequence analyses of the viral DNA isolated from a recent outbreak of disease in 1999–2000 are presented in comparison to that isolated from earlier outbreaks in 1992–1994. The results show that the Virus isolated from the 1999–2000 outbreak is most closely related to Pseudocowpox Virus, whereas those from previous years grouped with ORFV. The present study describes a method for genetic characterization and classification of parapoxViruses (PPVs) and provides for the first time an extended phylogenetic analysis of PPVs isolated from Finland, established members of the genus ParapoxVirus and selected members of the subfamily Chordopoxvirinae.
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Short Communication Recent isolates of parapoxVirus of Finnish reindeer (Rangifer tarandus tarandus) are closely related to bovine Pseudocowpox Virus
2004Co-Authors: Maria K Tikkanen, Jarno Tuimala, Colin Mcinnes, Erkki Neuvonen, Anita HuovilainenAbstract:Cases of papular stomatitis in Finnish reindeer have been reported for many years. The causative agent was thought to be Orf Virus (ORFV), one of the Parapoxviridae, although this assumption was based mainly on clinical symptoms, pathology and electron microscopy. Here sequence analyses of the viral DNA isolated from a recent outbreak of disease in 1999–2000 are presented in comparison to that isolated from earlier outbreaks in 1992–1994. The results show that the Virus isolated from the 1999–2000 outbreak is most closely related to Pseudocowpox Virus, whereas those from previous years grouped with ORFV. The present study describes a method for genetic characterization and classification of parapoxViruses (PPVs) and provides for the first time an extended phylogenetic analysis of PPVs isolated from Finland, established members of the genus ParapoxVirus and selected members of the subfamily Chordopoxvirinae.
Hisato Narushige - One of the best experts on this subject based on the ideXlab platform.
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First isolation and genetic characterization of Pseudocowpox Virus from cattle in Japan.
Virology journal, 2017Co-Authors: Akifumi Ohtani, Akihiro Yokoyama, Hisato NarushigeAbstract:Pseudocowpox Virus (PCPV) infects cattle worldwide with zoonotic potential but has not been isolated in Japan. Thus, the epidemiological status of PCPV infection in cattle is undetermined. In May 2016, a cattle in a farm in Yamaguchi Prefecture showed white vesicles and hyperemia in the mucosa under the tongue surface, but not on the teats and coronary cushions. A parapoxVirus was isolated from the oral lesion swab and was genetically characterized based on the full-length sequence of B2L gene encoding viral envelope. Phylogenetic analysis showed that the isolated Virus was classified into PCPV. This case indicates its potential spread in Japan. This is the first report of isolation of PCPV in Japan.
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First isolation and genetic characterization of Pseudocowpox Virus from cattle in Japan
Virology Journal, 2017Co-Authors: Akifumi Ohtani, Akihiro Yokoyama, Hisato NarushigeAbstract:Background Pseudocowpox Virus (PCPV) infects cattle worldwide with zoonotic potential but has not been isolated in Japan. Thus, the epidemiological status of PCPV infection in cattle is undetermined. Results In May 2016, a cattle in a farm in Yamaguchi Prefecture showed white vesicles and hyperemia in the mucosa under the tongue surface, but not on the teats and coronary cushions. A parapoxVirus was isolated from the oral lesion swab and was genetically characterized based on the full-length sequence of B2L gene encoding viral envelope. Phylogenetic analysis showed that the isolated Virus was classified into PCPV. Conclusion This case indicates its potential spread in Japan. This is the first report of isolation of PCPV in Japan.
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Additional file 1: Figure S1. of First isolation and genetic characterization of Pseudocowpox Virus from cattle in Japan
2017Co-Authors: Akifumi Ohtani, Akihiro Yokoyama, Hisato NarushigeAbstract:Alignment of the deduced amino acid sequences of the full-length B2L gene. Amino acids identical to the Pseudocowpox Virus strain YG2828 at given positions are represented by dots. (PPTX 73Â kb
Maria K Tikkanen - One of the best experts on this subject based on the ideXlab platform.
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recent isolates of parapoxVirus of finnish reindeer rangifer tarandus tarandus are closely related to bovine Pseudocowpox Virus
Journal of General Virology, 2004Co-Authors: Maria K Tikkanen, Jarno Tuimala, Varpu Hirvelakoski, Colin Mcinnes, Mathias Büttner, Erkki Neuvonen, Anita HuovilainenAbstract:Cases of papular stomatitis in Finnish reindeer have been reported for many years. The causative agent was thought to be Orf Virus (ORFV), one of the Parapoxviridae, although this assumption was based mainly on clinical symptoms, pathology and electron microscopy. Here sequence analyses of the viral DNA isolated from a recent outbreak of disease in 1999–2000 are presented in comparison to that isolated from earlier outbreaks in 1992–1994. The results show that the Virus isolated from the 1999–2000 outbreak is most closely related to Pseudocowpox Virus, whereas those from previous years grouped with ORFV. The present study describes a method for genetic characterization and classification of parapoxViruses (PPVs) and provides for the first time an extended phylogenetic analysis of PPVs isolated from Finland, established members of the genus ParapoxVirus and selected members of the subfamily Chordopoxvirinae.
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Short Communication Recent isolates of parapoxVirus of Finnish reindeer (Rangifer tarandus tarandus) are closely related to bovine Pseudocowpox Virus
2004Co-Authors: Maria K Tikkanen, Jarno Tuimala, Colin Mcinnes, Erkki Neuvonen, Anita HuovilainenAbstract:Cases of papular stomatitis in Finnish reindeer have been reported for many years. The causative agent was thought to be Orf Virus (ORFV), one of the Parapoxviridae, although this assumption was based mainly on clinical symptoms, pathology and electron microscopy. Here sequence analyses of the viral DNA isolated from a recent outbreak of disease in 1999–2000 are presented in comparison to that isolated from earlier outbreaks in 1992–1994. The results show that the Virus isolated from the 1999–2000 outbreak is most closely related to Pseudocowpox Virus, whereas those from previous years grouped with ORFV. The present study describes a method for genetic characterization and classification of parapoxViruses (PPVs) and provides for the first time an extended phylogenetic analysis of PPVs isolated from Finland, established members of the genus ParapoxVirus and selected members of the subfamily Chordopoxvirinae.
Marcelo Fernandes Camargos - One of the best experts on this subject based on the ideXlab platform.
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spread of poxViruses in livestock in brazil associated with cases of double and triple infection
Archives of Virology, 2017Co-Authors: Mateus Laguardianascimento, Ana Paula Ferreira De Oliveira, Isabela Ciarlini De Azevedo, Anselmo Vasconcelos Rivetti, Marcelo Fernandes Camargos, Antônio Augusto FonsecaAbstract:The objective of this work is to describe the distribution of outbreaks of vaccinia Virus (VACV), Pseudocowpox Virus (PCPV), and bovine papular stomatitis Virus (BSPV) in Brazil. The Official Laboratory of the Brazilian Ministry of Agriculture received 89 samples from different locations in Brazil in 2015 and 2016 for diagnosis of vesicular and exanthematous disease. PoxVirus coinfections occurred in 11 out of 33 outbreaks, including the first reported triple infection by BPSV, PCPV, and VACV. This occurrence may be associated with the circulation of these Viruses in Brazilian cattle.
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Detection of Pseudocowpox Virus in water buffalo (Bubalus bubalis) with vesicular disease in the state of São Paulo, Brazil, in 2016
Taylor & Francis Group, 2017Co-Authors: Mateus Laguardia-nascimento, Ana Paula Ferreira De Oliveira, Anselmo Vasconcelos Rivetti, Marcelo Fernandes Camargos, Fernanda Rodas Pires Fernandes, Antônio Augusto Fonseca JúniorAbstract:Background: ParapoxViruses are zoonotic Viruses that infect cattle, goats and sheep; there have also been reports of infections in camels, domestic cats and seals. Objective: The objective of this report was to describe a case of vesicular disease caused by Pseudocowpox Virus (PCPV) in water buffalo (Bubalus bubalis) in Brazil. Animals: Sixty buffalo less than 6 months old exhibited ulcers and widespread peeling of the tongue epithelium. There were no cases of vesicular disease in pigs or horses on the same property. Methods: Samples were analysed by PCR and sequencing. Phylogenetic analysis in MEGA 7.01 was reconstructed using major envelope protein (B2L) by the Tamura three-parameter nucleotide substitution model and the maximum likelihood and neighbor joining models, both with 1000 bootstrap replicates. The genetic distance between the groups was analysed in MEGA using the maximum composite likelihood model. The rate variation among sites was modeled using gamma distribution. Results: The presence of PCPV in the buffalo herd could be demonstrated in epithelium and serum. The minimum genetic distance between the isolated PCPV strain (262-2016) and orf Virus and bovine papular stomatitis Virus was 6.7% and 18.4%, respectively. The maximum genetic distance calculated was 4.6% when compared with a PCPV detected in a camel. Conclusions/Clinical Importance: The peculiar position of the isolated strain in the phylogenetic trees does not necessarily indicate a different kind of PCPV that infects buffalo. More samples from cattle and buffalo in Brazil must be sequenced and compared to verify if PCPV from buffalo are genetically different from samples derived from cattle
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Detection of Pseudocowpox Virus in water buffalo (Bubalus bubalis) with vesicular disease in the state of São Paulo, Brazil, in 2016.
The veterinary quarterly, 2016Co-Authors: Mateus Laguardia-nascimento, Ana Paula Ferreira De Oliveira, Marcelo Fernandes Camargos, Fernanda Rodas Pires Fernandes, Anselmo Vasconcelos Rivetti Júnior, Antônio Augusto Fonseca JúniorAbstract:ABSTRACTBackground: ParapoxViruses are zoonotic Viruses that infect cattle, goats and sheep; there have also been reports of infections in camels, domestic cats and seals.Objective: The objective of this report was to describe a case of vesicular disease caused by Pseudocowpox Virus (PCPV) in water buffalo (Bubalus bubalis) in Brazil.Animals: Sixty buffalo less than 6 months old exhibited ulcers and widespread peeling of the tongue epithelium. There were no cases of vesicular disease in pigs or horses on the same property.Methods: Samples were analysed by PCR and sequencing. Phylogenetic analysis in MEGA 7.01 was reconstructed using major envelope protein (B2L) by the Tamura three-parameter nucleotide substitution model and the maximum likelihood and neighbor joining models, both with 1000 bootstrap replicates. The genetic distance between the groups was analysed in MEGA using the maximum composite likelihood model. The rate variation among sites was modeled using gamma distribution.Results: The presence ...
