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Yasuo Inoshima - One of the best experts on this subject based on the ideXlab platform.
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Bovine papular stomatitis virus and Pseudocowpox virus coinfection in dairy calves in Japan
Archives of Virology, 2020Co-Authors: Kaori Shimizu, Yassien Badr, Ayaka Okada, Yasuo InoshimaAbstract:Two cases of coinfection with bovine papular stomatitis virus (BPSV) and Pseudocowpox virus (PCPV) in dairy calves in Tochigi Prefecture, Japan, are reported. Sequences of BPSV and PCPV were simultaneously detected in the same polymerase chain reaction (PCR) amplicons, which were obtained from the DNA of two dairy calves using a pan-parapoxvirus primer set. PCR amplification using BPSV- and PCPV-specific primer sets were able to distinguish between the two viruses in coinfected clinical samples. Based on these data, further studies on the occurrence BPSV/PCPV coinfections in cattle in Japan are warranted.
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Sequential detection of Pseudocowpox virus and bovine papular stomatitis virus in a same calf in Japan.
The Journal of veterinary medical science, 2019Co-Authors: Haruna Matsumoto, Akifumi Ohtani, Kaori Shimizu, Ayaka Okada, Hironori Setoyama, Yuichi Matsuura, Yasuo InoshimaAbstract:We detected parapoxviruses from environmental samples and calves with and without intraoral clinical signs and conducted molecular and serological analyses. Pseudocowpox virus (PCPV) was detected from a calf showing anorexia, frothy salivation, and erosion in the mucosa of the lip and tongue. At the time that PCPV was detected, bovine papular stomatitis viruses (BPSVs) were detected in environmental samples as well as in calves without intraoral clinical signs. BPSV, but not PCPV, was detected in the same calf after 22 days. Phylogenetic analysis revealed that genetically different PCPV strains exist in Japan. This is the first report on the detection of PCPV and BPSV sequentially in the same calf and coexistence of PCPV and BPSV in the same farm in Japan.
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First isolation and genetic characterization of Pseudocowpox virus from cattle in Japan
Virology Journal, 2017Co-Authors: Akifumi Ohtani, Akihiro Yokoyama, Hisato Narushige, Yasuo InoshimaAbstract:Background Pseudocowpox virus (PCPV) infects cattle worldwide with zoonotic potential but has not been isolated in Japan. Thus, the epidemiological status of PCPV infection in cattle is undetermined. Results In May 2016, a cattle in a farm in Yamaguchi Prefecture showed white vesicles and hyperemia in the mucosa under the tongue surface, but not on the teats and coronary cushions. A parapoxvirus was isolated from the oral lesion swab and was genetically characterized based on the full-length sequence of B2L gene encoding viral envelope. Phylogenetic analysis showed that the isolated virus was classified into PCPV. Conclusion This case indicates its potential spread in Japan. This is the first report of isolation of PCPV in Japan.
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First isolation and genetic characterization of Pseudocowpox virus from cattle in Japan.
Virology journal, 2017Co-Authors: Akifumi Ohtani, Akihiro Yokoyama, Hisato Narushige, Yasuo InoshimaAbstract:Pseudocowpox virus (PCPV) infects cattle worldwide with zoonotic potential but has not been isolated in Japan. Thus, the epidemiological status of PCPV infection in cattle is undetermined. In May 2016, a cattle in a farm in Yamaguchi Prefecture showed white vesicles and hyperemia in the mucosa under the tongue surface, but not on the teats and coronary cushions. A parapoxvirus was isolated from the oral lesion swab and was genetically characterized based on the full-length sequence of B2L gene encoding viral envelope. Phylogenetic analysis showed that the isolated virus was classified into PCPV. This case indicates its potential spread in Japan. This is the first report of isolation of PCPV in Japan.
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Additional file 1: Figure S1. of First isolation and genetic characterization of Pseudocowpox virus from cattle in Japan
2017Co-Authors: Akifumi Ohtani, Akihiro Yokoyama, Hisato Narushige, Yasuo InoshimaAbstract:Alignment of the deduced amino acid sequences of the full-length B2L gene. Amino acids identical to the Pseudocowpox virus strain YG2828 at given positions are represented by dots. (PPTX 73Â kb
Andrew A Mercer - One of the best experts on this subject based on the ideXlab platform.
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Persistent Pseudocowpox virus infection of the skin of a foot in a cat.
New Zealand veterinary journal, 2013Co-Authors: R. A. Fairley, Andrew A Mercer, C I Copland, S M Craig, P A HeslipAbstract:Pseudocowpox virus infects the skin of the teats and udders of cows. It is one of four parapoxviruses present in New Zealand animals along with bovine papular stomatitis virus, parapoxvirus of red ...
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Conservation and variation of the parapoxvirus GM-CSF-inhibitory factor (GIF) proteins
Journal of General Virology, 2009Co-Authors: David Deane, Andrew A Mercer, Norihito Ueda, Lyn M. Wise, Stephen B. Fleming, Ann R. Wood, Ann Percival, C. Jepson, Neil F. Inglis, Colin McinnesAbstract:The GIF protein of orf virus (ORFV) binds and inhibits the ovine cytokines granulocyte–macrophage colony-stimulating factor (GM-CSF) and interleukin-2 (IL-2). An equivalent protein has so far not been found in any of the other poxvirus genera and we therefore investigated whether it was conserved in the parapoxviruses. The corresponding genes from both the bovine-specific Pseudocowpox virus (PCPV) and bovine papular stomatitis virus (BPSV) were cloned and sequenced. The predicted amino acid sequences of the PCPV and BPSV proteins shared 88 and 37 % identity, respectively, with the ORFV protein. Both retained the six cysteine residues and the WSXWS-like motif that are required for biological activity of the ORFV protein. However, an analysis of the biological activity of the two recombinant proteins revealed that, whilst the PCPV GIF protein bound to both ovine and bovine GM-CSF and IL-2 with very similar binding affinities to the ORFV GIF protein, no GM-CSF- or IL-2-binding activity was found for the BPSV protein.
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recent isolates of parapoxvirus of finnish reindeer rangifer tarandus tarandus are closely related to bovine Pseudocowpox virus
Journal of General Virology, 2004Co-Authors: Maria K Tikkanen, Andrew A Mercer, Jarno Tuimala, Varpu Hirvelakoski, Colin Mcinnes, Mathias Büttner, Erkki Neuvonen, Anita HuovilainenAbstract:Cases of papular stomatitis in Finnish reindeer have been reported for many years. The causative agent was thought to be Orf virus (ORFV), one of the Parapoxviridae, although this assumption was based mainly on clinical symptoms, pathology and electron microscopy. Here sequence analyses of the viral DNA isolated from a recent outbreak of disease in 1999–2000 are presented in comparison to that isolated from earlier outbreaks in 1992–1994. The results show that the virus isolated from the 1999–2000 outbreak is most closely related to Pseudocowpox virus, whereas those from previous years grouped with ORFV. The present study describes a method for genetic characterization and classification of parapoxviruses (PPVs) and provides for the first time an extended phylogenetic analysis of PPVs isolated from Finland, established members of the genus Parapoxvirus and selected members of the subfamily Chordopoxvirinae.
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Short Communication Recent isolates of parapoxvirus of Finnish reindeer (Rangifer tarandus tarandus) are closely related to bovine Pseudocowpox virus
2004Co-Authors: Maria K Tikkanen, Andrew A Mercer, Jarno Tuimala, Colin Mcinnes, Erkki Neuvonen, Anita HuovilainenAbstract:Cases of papular stomatitis in Finnish reindeer have been reported for many years. The causative agent was thought to be Orf virus (ORFV), one of the Parapoxviridae, although this assumption was based mainly on clinical symptoms, pathology and electron microscopy. Here sequence analyses of the viral DNA isolated from a recent outbreak of disease in 1999–2000 are presented in comparison to that isolated from earlier outbreaks in 1992–1994. The results show that the virus isolated from the 1999–2000 outbreak is most closely related to Pseudocowpox virus, whereas those from previous years grouped with ORFV. The present study describes a method for genetic characterization and classification of parapoxviruses (PPVs) and provides for the first time an extended phylogenetic analysis of PPVs isolated from Finland, established members of the genus Parapoxvirus and selected members of the subfamily Chordopoxvirinae.
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Pseudocowpox virus Encodes a Homolog of Vascular Endothelial Growth Factor
Virology, 2003Co-Authors: Norihito Ueda, Lyn M. Wise, Steven A. Stacker, Stephen B. Fleming, Andrew A MercerAbstract:Abstract We have identified a gene encoding a homolog of vascular endothelial growth factor (VEGF) in the Pseudocowpox virus (PCPV) genome. The predicted protein shows 27% amino acid identity to human VEGF-A. It also shows 41 and 61% amino acid identity to VEGFs encoded by orf virus (ORFV) strains NZ2 and NZ7, respectively. Assays of the expressed VEGF-like protein of PCPV (PCPV VR634 VEGF) demonstrated that PCPV VR634 VEGF is mitogenic for endothelial cells and is capable of inducing vascular permeability. PCPV VR634 VEGF bound VEGF receptor-2 (VEGFR-2) but did not bind VEGFR-1 or VEGFR-3. These results indicate that PCPV VR634 VEGF is a biologically active member of the VEGF family which shares with the ORFV-encoded VEGFs a receptor binding profile that differs from those of all cellular members of the VEGF family. It seems likely that the biological activities of PCPV VR634 VEGF contribute to the proliferative and highly vascularized nature of PCPV lesions.
Charles Euloge Lamien - One of the best experts on this subject based on the ideXlab platform.
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First detection and molecular characterisation of Pseudocowpox virus in a cattle herd in Zambia
Virology Journal, 2020Co-Authors: Maureen Wakwamba Ziba, Chanda Chitala, Tirumala Bharani K Settypalli, Malama Mumba, Giovanni Cattoli, Paul Fandamu, Charles Euloge LamienAbstract:Background Pseudocowpox virus (PCPV) of the genus Parapoxvirus in the family Poxviridae causes Pseudocowpox in cattle worldwide and presents a zoonotic concern. Most poxviruses produce diseases of similar clinical signs in affected animals, which are impossible to differentiate clinically or by serology. It is, therefore, vital to use molecular assays to rapidly identify the causative agents of poxvirus infections. This study aimed to detect, diagnose, and characterize the causative agent of pox-like skin lesions in a cattle herd in Zambia, initially suspected to be infected with Lumpy Skin Disease virus. Methods We used a High-Resolution Melting (HRM) analysis assay to detect the PCPV genome and sequenced the major envelope protein (B2L gene) for comparative sequence and phylogenetic analysis. Results Our field investigations showed cattle presenting atypical skin lesions and high morbidity within the herd. The laboratory diagnosis, based on the HRM assay revealed PCPV DNA in the samples. Phylogenetic and comparative sequence analyses confirmed PCPV in the samples and revealed genomic differences between samples collected in 2017 and 2018 from the same farm. Conclusion Our work is the first documented report of PCPV in Zambia. It shows the strength of molecular methods to diagnose pox-like infections in cattle and discriminate between diseases causing similar clinical signs. This rapid and accurate diagnosis improves the response time for more accurate veterinary interventions.
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First Detection and Molecular Characterisation of Pseudocowpox Virus in a Cattle Herd in Zambia
2020Co-Authors: Maureen Wakwamba Ziba, Chanda Chitala, Tirumala Bharani K Settypalli, Malama Mumba, Giovanni Cattoli, Paul Fandamu, Charles Euloge LamienAbstract:Abstract Background: Pseudocowpox virus (PCPV) of the genus Parapoxvirus in the family Poxviridae causes Pseudocowpox in cattle worldwide and presents a zoonotic concern. Most poxviruses produce diseases of similar clinical signs in affected animals, which are impossible to differentiate clinically or by serology. It is, therefore, vital to use molecular assays to identify the causative agents of poxvirus infections rapidly. This study aimed to detect, diagnose, and characterize the causative agent of pox-like skin lesions in a cattle herd in Zambia, initially suspected to be infected with the Lumpy Skin Disease virus.Methods: We used a high resolution melting (HRM) analysis assay to detect the PCPV genome and sequenced the major envelope protein (B2L) gene for comparative sequence and phylogenetic analysis. Results: Our field investigations showed cattle presenting atypical skin lesions and high morbidity within the herd. The laboratory diagnosis based on an HRM assay revealed the PCPV genome in the samples. Phylogenetic and comparative sequence analyses confirmed PCPV in the samples. They revealed genomic differences between samples collected in 2017 and 2018 from the same farm.Conclusion: Our work is the first documented report of PCPV in Zambia. It shows the strength of molecular methods to diagnose pox-like infections in cattle and discriminate between diseases causing similar clinical signs for better veterinary interventions.
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A novel HRM assay for the simultaneous detection and differentiation of eight poxviruses of medical and veterinary importance
Scientific Reports, 2017Co-Authors: Esayas Gelaye, Angelika Loitsch, Jenna Elizabeth Achenbach, Adama Diallo, Lukas Mach, Norbert Nowotny, Jolanta Kolodziejek, Reingard Grabherr, Charles Euloge LamienAbstract:Poxviruses belonging to the Orthopoxvirus, Capripoxvirus and Parapoxvirus genera share common host species and create a challenge for diagnosis. Here, we developed a novel multiplex PCR method for the simultaneous detection and differentiation of eight poxviruses, belonging to three genera: cowpox virus (CPXV) and camelpox virus (CMLV) [genus Orthopoxvirus ]; goatpox virus (GTPV), sheeppox virus (SPPV) and lumpy skin disease virus (LSDV) [genus Capripoxvirus ]; orf virus (ORFV), Pseudocowpox virus (PCPV) and bovine papular stomatitis virus (BPSV) [genus Parapoxvirus ]. The assay is based on high-resolution melting curve analysis (HRMCA) of PCR amplicons produced using genus specific primer pairs and dsDNA binding dye. Differences in fragment size and GC content were used as discriminating power. The assay generated three well separated melting regions for each genus and provided additional intra-genus genotyping allowing the differentiation of the eight poxviruses based on amplicon melting temperature. Out of 271 poxviral DNA samples tested: seven CPXV, 25 CMLV, 42 GTPV, 20 SPPV, 120 LSDV, 33 ORFV, 20 PCPV and two BPSV were detected; two samples presented co-infection with CMLV and PCPV. The assay provides a rapid, sensitive, specific and cost-effective method for the detection of pox diseases in a broad range of animal species and humans.
Abdullah I. A. Al-mubarak - One of the best experts on this subject based on the ideXlab platform.
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Phylogenetic analysis of eight sudanese camel contagious ecthyma viruses based on B2L gene sequence
Virology journal, 2015Co-Authors: Abdelmalik I. Khalafalla, Ibrahim M. El-sabagh, Khalid A. Al-busada, Abdullah I. A. Al-mubarak, Yahia Hassan AliAbstract:Camel contagious ecthyma (CCE) is an important viral disease of camelids caused by a poxvirus of the genus parapoxvirus (PPV) of the family Poxviridae. The disease has been reported in west and east of the Sudan causing economical losses. However, the PPVs that cause the disease in camels of the Sudan have not yet subjected to genetic characterization. At present, the PPV that cause CCE cannot be properly classified because only few isolates that have been genetically analyzed. PCR was used to amplify the B2L gene of the PPV directly from clinical specimens collected from dromedary camels affected with contagious ecthyma in the Sudan between 1993 and 2013. PCR products were sequenced and subjected to genetic analysis. The results provided evidence for close relationships and genetic variation of the camel PPV (CPPV) represented by the circulation of both Pseudocowpox virus (PCPV) and Orf virus (ORFV) strains among dromedary camels in the Sudan. Based on the B2L gene sequence the available CPPV isolates can be divided into two genetic clades or lineages; the Asian lineage represented by isolates from Saudi Arabia, Bahrain and India and the African lineage comprising isolates from the Sudan. The camel parapoxvirus is genetically diverse involving predominantly viruses close to PCPV in addition to ORFVs, and can be divided into two genetically distant lineages. Based on sequences of the B2L gene it is not possible to suggest that the viruses that cause CCE form a monophylogenetic group or species within the PPV phylogeny.
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Phylogenetic analysis of eight sudanese camel contagious ecthyma viruses based on B2L gene sequence
Virology Journal, 2015Co-Authors: Abdelmalik I. Khalafalla, Ibrahim M. El-sabagh, Khalid A. Al-busada, Abdullah I. A. Al-mubarakAbstract:Background Camel contagious ecthyma (CCE) is an important viral disease of camelids caused by a poxvirus of the genus parapoxvirus (PPV) of the family Poxviridae . The disease has been reported in west and east of the Sudan causing economical losses. However, the PPVs that cause the disease in camels of the Sudan have not yet subjected to genetic characterization. At present, the PPV that cause CCE cannot be properly classified because only few isolates that have been genetically analyzed. Methods and results PCR was used to amplify the B2L gene of the PPV directly from clinical specimens collected from dromedary camels affected with contagious ecthyma in the Sudan between 1993 and 2013. PCR products were sequenced and subjected to genetic analysis. The results provided evidence for close relationships and genetic variation of the camel PPV (CPPV) represented by the circulation of both Pseudocowpox virus (PCPV) and Orf virus (ORFV) strains among dromedary camels in the Sudan. Based on the B2L gene sequence the available CPPV isolates can be divided into two genetic clades or lineages; the Asian lineage represented by isolates from Saudi Arabia, Bahrain and India and the African lineage comprising isolates from the Sudan. Conclusion The camel parapoxvirus is genetically diverse involving predominantly viruses close to PCPV in addition to ORFVs, and can be divided into two genetically distant lineages. Based on sequences of the B2L gene it is not possible to suggest that the viruses that cause CCE form a monophylogenetic group or species within the PPV phylogeny.
Marcelo Fernandes Camargos - One of the best experts on this subject based on the ideXlab platform.
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spread of poxviruses in livestock in brazil associated with cases of double and triple infection
Archives of Virology, 2017Co-Authors: Mateus Laguardianascimento, Ana Paula Ferreira De Oliveira, Isabela Ciarlini De Azevedo, Anselmo Vasconcelos Rivetti, Marcelo Fernandes Camargos, Antônio Augusto FonsecaAbstract:The objective of this work is to describe the distribution of outbreaks of vaccinia virus (VACV), Pseudocowpox virus (PCPV), and bovine papular stomatitis virus (BSPV) in Brazil. The Official Laboratory of the Brazilian Ministry of Agriculture received 89 samples from different locations in Brazil in 2015 and 2016 for diagnosis of vesicular and exanthematous disease. Poxvirus coinfections occurred in 11 out of 33 outbreaks, including the first reported triple infection by BPSV, PCPV, and VACV. This occurrence may be associated with the circulation of these viruses in Brazilian cattle.
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Detection of Pseudocowpox virus in water buffalo (Bubalus bubalis) with vesicular disease in the state of São Paulo, Brazil, in 2016
Taylor & Francis Group, 2017Co-Authors: Mateus Laguardia-nascimento, Ana Paula Ferreira De Oliveira, Anselmo Vasconcelos Rivetti, Marcelo Fernandes Camargos, Fernanda Rodas Pires Fernandes, Antônio Augusto Fonseca JúniorAbstract:Background: Parapoxviruses are zoonotic viruses that infect cattle, goats and sheep; there have also been reports of infections in camels, domestic cats and seals. Objective: The objective of this report was to describe a case of vesicular disease caused by Pseudocowpox virus (PCPV) in water buffalo (Bubalus bubalis) in Brazil. Animals: Sixty buffalo less than 6 months old exhibited ulcers and widespread peeling of the tongue epithelium. There were no cases of vesicular disease in pigs or horses on the same property. Methods: Samples were analysed by PCR and sequencing. Phylogenetic analysis in MEGA 7.01 was reconstructed using major envelope protein (B2L) by the Tamura three-parameter nucleotide substitution model and the maximum likelihood and neighbor joining models, both with 1000 bootstrap replicates. The genetic distance between the groups was analysed in MEGA using the maximum composite likelihood model. The rate variation among sites was modeled using gamma distribution. Results: The presence of PCPV in the buffalo herd could be demonstrated in epithelium and serum. The minimum genetic distance between the isolated PCPV strain (262-2016) and orf virus and bovine papular stomatitis virus was 6.7% and 18.4%, respectively. The maximum genetic distance calculated was 4.6% when compared with a PCPV detected in a camel. Conclusions/Clinical Importance: The peculiar position of the isolated strain in the phylogenetic trees does not necessarily indicate a different kind of PCPV that infects buffalo. More samples from cattle and buffalo in Brazil must be sequenced and compared to verify if PCPV from buffalo are genetically different from samples derived from cattle
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Detection of Pseudocowpox virus in water buffalo (Bubalus bubalis) with vesicular disease in the state of São Paulo, Brazil, in 2016.
The veterinary quarterly, 2016Co-Authors: Mateus Laguardia-nascimento, Ana Paula Ferreira De Oliveira, Marcelo Fernandes Camargos, Fernanda Rodas Pires Fernandes, Anselmo Vasconcelos Rivetti Júnior, Antônio Augusto Fonseca JúniorAbstract:ABSTRACTBackground: Parapoxviruses are zoonotic viruses that infect cattle, goats and sheep; there have also been reports of infections in camels, domestic cats and seals.Objective: The objective of this report was to describe a case of vesicular disease caused by Pseudocowpox virus (PCPV) in water buffalo (Bubalus bubalis) in Brazil.Animals: Sixty buffalo less than 6 months old exhibited ulcers and widespread peeling of the tongue epithelium. There were no cases of vesicular disease in pigs or horses on the same property.Methods: Samples were analysed by PCR and sequencing. Phylogenetic analysis in MEGA 7.01 was reconstructed using major envelope protein (B2L) by the Tamura three-parameter nucleotide substitution model and the maximum likelihood and neighbor joining models, both with 1000 bootstrap replicates. The genetic distance between the groups was analysed in MEGA using the maximum composite likelihood model. The rate variation among sites was modeled using gamma distribution.Results: The presence ...
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Detection of multiple viral infections in cattle and buffalo with suspected vesicular disease in Brazil
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians Inc, 2016Co-Authors: Mateus Laguardia-nascimento, Anselmo Vasconcelos Rivetti Júnior, Érica Bravo Sales, Marcela Ribeiro Gasparini, Natália Mendes De Souza, Josiane Aparecida Gonçalina Da Silva, Giovana Gonçalves Souza, Fernanda Rezek Carani, Alyane Figueiredo Dos Santos, Marcelo Fernandes CamargosAbstract:Vesicular diseases are of high importance for livestock, primarily because of foot-and-mouth disease (FMD), which is a high-morbidity disease that generates direct losses caused by low milk production, weight loss, and indirect losses because of the need for sanitary barriers. Other vesicular diseases are also of importance for livestock because of direct impacts or because their clinical signs may be confused with those of FMD. We report herein the detection of multiple infections in cattle with suspected vesicular disease in the Brazilian states of Amazonas (AM), Mato Grosso (MT), and Roraima. Thirty-seven epithelial samples from cattle and 1 sample from a buffalo were sent to the laboratory for testing for FMDV and similar disease agents. All samples from MT were positive for parapoxvirus (Pseudocowpox virus and Bovine papular stomatitis virus). In addition, 3 samples were positive for Bluetongue virus, and 5 samples were positive for Bovine herpesvirus 1. Among these samples, 1 was positive for all of...
