Pseudoinclusion

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Teresa Lorenzi - One of the best experts on this subject based on the ideXlab platform.

  • Granular osmiophilic material-containing Pseudoinclusions in CADASIL.
    Journal of neuropathology and experimental neurology, 2014
    Co-Authors: Manrico Morroni, Teresa Lorenzi, Mario Castellucci, Michele Ragno, Marina Scarpelli
    Abstract:

    We recently described the role of electron microscopy examination in the diagnosis of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) syndrome in a retrospective study of 13 cases (1). In 1 patient who was homozygous for the G528C mutation (2), vascular smooth muscle cells (VSMCs) showed several cytoplasmic inclusions that were found to be granular osmiophilic material (GOM)–containing Pseudoinclusions; the other 12 heterozygous patients did not seem to be affected. To determine whether GOM-containing Pseudoinclusions are a pathognomonic sign of CADASIL syndrome, particularly of the homozygous condition, we retrieved from our archives biopsy samples from all 13 patients (8 men and 5 women; age range, 45–64 years; mean, 53 years) with a diagnosis of CADASIL disease and re-examined them by electron microscopy to assess GOM-containing Pseudoinclusions both qualitatively and quantitatively. Patients are listed in the Table; age, sex, sample type, molecular findings, and genotype are presented by Morroni et al (1). Patient 12 was homozygous for the G528C mutation (2). View this table: TABLE Extent of VSMC Branching and Proportion of Cells Showing GOM-Containing Pseudoinclusions in 13 CADASIL Patients Granular osmiophilic material–containing Pseudoinclusions were quantified by examining 50 nucleated VSMCs or VSMCs devoid of the nucleus but containing abundant cytoplasm in small and medium arteries of each patient sample and by calculating the proportion of those bearing GOM-containing Pseudoinclusions. Vascular smooth muscle cell cytoplasmic processes were not included in the calculation. Each Pseudoinclusion was surrounded by a cell membrane and separated from it by an electron-lucent halo (Fig. A). The cytoplasmic membrane showed numerous pinocytotic vesicles (Fig. A) that …

  • CADASIL: intracytoplasmic granular osmiophilic material deposits are Pseudoinclusions.
    Journal of neuropathology and experimental neurology, 2013
    Co-Authors: Manrico Morroni, Teresa Lorenzi
    Abstract:

    We read with interest the recent study by Yamamoto et al (1) in which the authors demonstrated the specificity of anti-NOTCH3 ectodomain antibodies in CADASIL patients and confirmed the predominant localization of NOTCH3 ectodomain in granular osmiophilic material (GOM) deposits. The authors also documented widespread accumulation and distribution of NOTCH3 ectodomain in the meninges and cerebral microcirculation, particularly in capillaries. In particular, Figure 7B shows a perivascular cell that …

Manrico Morroni - One of the best experts on this subject based on the ideXlab platform.

  • Granular osmiophilic material-containing Pseudoinclusions in CADASIL.
    Journal of neuropathology and experimental neurology, 2014
    Co-Authors: Manrico Morroni, Teresa Lorenzi, Mario Castellucci, Michele Ragno, Marina Scarpelli
    Abstract:

    We recently described the role of electron microscopy examination in the diagnosis of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) syndrome in a retrospective study of 13 cases (1). In 1 patient who was homozygous for the G528C mutation (2), vascular smooth muscle cells (VSMCs) showed several cytoplasmic inclusions that were found to be granular osmiophilic material (GOM)–containing Pseudoinclusions; the other 12 heterozygous patients did not seem to be affected. To determine whether GOM-containing Pseudoinclusions are a pathognomonic sign of CADASIL syndrome, particularly of the homozygous condition, we retrieved from our archives biopsy samples from all 13 patients (8 men and 5 women; age range, 45–64 years; mean, 53 years) with a diagnosis of CADASIL disease and re-examined them by electron microscopy to assess GOM-containing Pseudoinclusions both qualitatively and quantitatively. Patients are listed in the Table; age, sex, sample type, molecular findings, and genotype are presented by Morroni et al (1). Patient 12 was homozygous for the G528C mutation (2). View this table: TABLE Extent of VSMC Branching and Proportion of Cells Showing GOM-Containing Pseudoinclusions in 13 CADASIL Patients Granular osmiophilic material–containing Pseudoinclusions were quantified by examining 50 nucleated VSMCs or VSMCs devoid of the nucleus but containing abundant cytoplasm in small and medium arteries of each patient sample and by calculating the proportion of those bearing GOM-containing Pseudoinclusions. Vascular smooth muscle cell cytoplasmic processes were not included in the calculation. Each Pseudoinclusion was surrounded by a cell membrane and separated from it by an electron-lucent halo (Fig. A). The cytoplasmic membrane showed numerous pinocytotic vesicles (Fig. A) that …

  • CADASIL: intracytoplasmic granular osmiophilic material deposits are Pseudoinclusions.
    Journal of neuropathology and experimental neurology, 2013
    Co-Authors: Manrico Morroni, Teresa Lorenzi
    Abstract:

    We read with interest the recent study by Yamamoto et al (1) in which the authors demonstrated the specificity of anti-NOTCH3 ectodomain antibodies in CADASIL patients and confirmed the predominant localization of NOTCH3 ectodomain in granular osmiophilic material (GOM) deposits. The authors also documented widespread accumulation and distribution of NOTCH3 ectodomain in the meninges and cerebral microcirculation, particularly in capillaries. In particular, Figure 7B shows a perivascular cell that …

John K. C. Chan - One of the best experts on this subject based on the ideXlab platform.

  • Nuclear Inclusions and Pseudoinclusions: Friends or Foes of the Surgical Pathologist?
    2016
    Co-Authors: Marco Antonio, Dias Filho, John K. C. Chan
    Abstract:

    Abnormal substances in the nuclei that can be observed by light microscopy are often broadly referred to as nuclear inclusions. Although their recognition in the appropriate clinicopathological settings can aid in the diagnosis of some disease entities and tumor types, they can also be a source of error. There are 2 morphologically distinct types of inclu-sions with different mechanisms of formation and diagnostic significance, including bona fide nuclear inclusions and nuclear Pseudoinclusions. Bona fide nuclear inclusions result from accumulation in the nuclei of viral particles, cytoplasmic materials (such as surfactant, immunoglobulin, and glycogen), biotin, nuclear lamins, or polyglutamine. Some of them are diagnosti-cally helpful, such as surfactant inclusion, which can support the pulmonary origin of an adenocarcinoma, whereas others may be misleading, such as biotin inclusion, which can be mistaken for herpes infection. Nuclear Pseudoinclusions, which represent invaginations of cytoplasm into the nucleus, are delimited by the nuclear membrane. Although not totally specific, they are particularly common in papillary thyroid carcinoma, meningioma, and usual ductal hyperplasia of the breast and hence may aid in the diagnosis of these entities. Nuclear pseudo-Pseudoinclusions, which are artefactual bubbles in the nuclei that mimic nuclear Pseudoinclusions or clear nuclei, can lead to misdiagnosis of follicular adenoma or hyperplastic nodule as papillary thyroid carcinoma. Keywords nuclear inclusion, nuclear Pseudoinclusion, nuclear pseudo-Pseudoinclusion, viral inclusion, endogenous biotin, immunohistochemistr

  • Nuclear Inclusions and Pseudoinclusions: Friends or Foes of the Surgical Pathologist?:
    International journal of surgical pathology, 2010
    Co-Authors: Marco Antonio Dias Filho, John K. C. Chan
    Abstract:

    Abnormal substances in the nuclei that can be observed by light microscopy are often broadly referred to as nuclear inclusions. Although their recognition in the appropriate clinicopathological settings can aid in the diagnosis of some disease entities and tumor types, they can also be a source of error. There are 2 morphologically distinct types of inclusions with different mechanisms of formation and diagnostic significance, including bona fide nuclear inclusions and nuclear Pseudoinclusions. Bona fide nuclear inclusions result from accumulation in the nuclei of viral particles, cytoplasmic materials (such as surfactant, immunoglobulin, and glycogen), biotin, nuclear lamins, or polyglutamine. Some of them are diagnostically helpful, such as surfactant inclusion, which can support the pulmonary origin of an adenocarcinoma, whereas others may be misleading, such as biotin inclusion, which can be mistaken for herpes infection. Nuclear Pseudoinclusions, which represent invaginations of cytoplasm into the nu...

  • Follicular dendritic cell tumors of the oral cavity
    The American journal of surgical pathology, 1994
    Co-Authors: John K. C. Chan, William Y.w. Tsang, S. K. Tang, A. W M Lee
    Abstract:

    Follicular dendritic cell tumors are uncommon, and all the reported cases have occurred as primary lymph node tumors. We report two cases in the oral cavity, one in the soft palate and one in the tonsil. The tumors were characterized by sheets, whorls, and storiform arrays of spindly and syncytial-appearing cells with oval nuclei, fine chromatin, distinct nucleoli, and occasional nuclear Pseudoinclusions. Multinucleated forms were present and were prominent in one case. An unusual feature was the presence of irregular pseudovascular spaces, which could raise a concern for vascular neoplasm. Because the tumors showed cohesive growth and a sharp interface with the fibrous stroma, they could also be mistaken for carcinoma, sarcoma, or melanoma. After radiation therapy, the palatal tumor showed a greater degree of nuclear pleomorphism, numerous nuclear Pseudoinclusions, and striking nuclear grooving and foldings, mimicking interdigitating reticulum cell tumors. The diagnosis in both cases was confirmed by immunoreactivity with CD21 and CD35 and by ultrastructural demonstration of interdigitating cell processes with desmosomes. Both tumors also showed unexpected immunoreactivity with muscle-specific actin. Follicular dendritic cell tumor merits wider recognition of its possible extranodal occurrence as well as its full morphological spectrum in order to better define its behavior.

Leslie G Dodd - One of the best experts on this subject based on the ideXlab platform.

  • Acral myxoinflammatory fibroblastic sarcoma fine needle aspiration: a case report.
    Diagnostic cytopathology, 2011
    Co-Authors: M Quinn Wickham, Kenneth E Youens, Leslie G Dodd
    Abstract:

    Cytological diagnosis of low grade sarcomas can be a daunting task, owing to the varied cytomorphological appearances possible. We report a case of acral myxoinflammatory fibroblastic sarcoma (AMIFS) in a woman who presented with a longstanding mass on the dorsum of her left foot. The diagnosis was suggested by fine needle aspiration cytology and established by wide excision. Microscopic examination showed that fine needle aspirate smears of this lesion contained the characteristic features seen in the surgical excision of this AMIFS: myxoid material, spindled to epithelioid cells with variably prominent nucleoli, nuclear Pseudoinclusions, bipolar cytoplasmic extensions, globules of extracellular material, and bizarre virocyte or ganglion-like giant cells.

  • Acral myxoinflammatory fibroblastic sarcoma fine needle aspiration: A case report
    Diagnostic Cytopathology, 2011
    Co-Authors: M Quinn Wickham, Kenneth E Youens, Leslie G Dodd
    Abstract:

    Cytological diagnosis of low grade sarcomas can be a daunting task, owing to the varied cytomorphological appearances possible. We report a case of acral myxoinflammatory fibroblastic sarcoma (AMIFS) in a woman who presented with a longstanding mass on the dorsum of her left foot. The diagnosis was suggested by fine needle aspiration cytology and established by wide excision. Microscopic examination showed that fine needle aspirate smears of this lesion contained the characteristic features seen in the surgical excision of this AMIFS: myxoid material, spindled to epithelioid cells with variably prominent nucleoli, nuclear Pseudoinclusions, bipolar cytoplasmic extensions, globules of extracellular material, and bizarre virocyte or ganglion-like giant cells. Diagn. Cytopathol. 2012. © 2012 Wiley Periodicals, Inc.

Marina Scarpelli - One of the best experts on this subject based on the ideXlab platform.

  • Granular osmiophilic material-containing Pseudoinclusions in CADASIL.
    Journal of neuropathology and experimental neurology, 2014
    Co-Authors: Manrico Morroni, Teresa Lorenzi, Mario Castellucci, Michele Ragno, Marina Scarpelli
    Abstract:

    We recently described the role of electron microscopy examination in the diagnosis of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) syndrome in a retrospective study of 13 cases (1). In 1 patient who was homozygous for the G528C mutation (2), vascular smooth muscle cells (VSMCs) showed several cytoplasmic inclusions that were found to be granular osmiophilic material (GOM)–containing Pseudoinclusions; the other 12 heterozygous patients did not seem to be affected. To determine whether GOM-containing Pseudoinclusions are a pathognomonic sign of CADASIL syndrome, particularly of the homozygous condition, we retrieved from our archives biopsy samples from all 13 patients (8 men and 5 women; age range, 45–64 years; mean, 53 years) with a diagnosis of CADASIL disease and re-examined them by electron microscopy to assess GOM-containing Pseudoinclusions both qualitatively and quantitatively. Patients are listed in the Table; age, sex, sample type, molecular findings, and genotype are presented by Morroni et al (1). Patient 12 was homozygous for the G528C mutation (2). View this table: TABLE Extent of VSMC Branching and Proportion of Cells Showing GOM-Containing Pseudoinclusions in 13 CADASIL Patients Granular osmiophilic material–containing Pseudoinclusions were quantified by examining 50 nucleated VSMCs or VSMCs devoid of the nucleus but containing abundant cytoplasm in small and medium arteries of each patient sample and by calculating the proportion of those bearing GOM-containing Pseudoinclusions. Vascular smooth muscle cell cytoplasmic processes were not included in the calculation. Each Pseudoinclusion was surrounded by a cell membrane and separated from it by an electron-lucent halo (Fig. A). The cytoplasmic membrane showed numerous pinocytotic vesicles (Fig. A) that …

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