The Experts below are selected from a list of 1842 Experts worldwide ranked by ideXlab platform
Alessio Giacomini - One of the best experts on this subject based on the ideXlab platform.
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comparative evaluation of cheese whey microbial composition from four italian cheese factories by viable counts and 16s rrna gene amplicon sequencing
International Dairy Journal, 2020Co-Authors: Vinicius Da Silva Duarte, Milena Carlot, Shadi Pakroo, Armin Tarrah, Angiolella Lombardi, Helena Santiago, Viviana Corich, Alessio GiacominiAbstract:Abstract The microbiota of cheese whey from four dairy companies located in the North-East of Italy was evaluated during winter and spring using both culture-dependent and culture-independent methods. Although no significant differences were observed (p > 0.05) for any of the six microbial categories analysed by plate counting among the dairy companies, significant differences at the family and genus level were revealed by linear discriminant analysis (LDA). In particular, variations in the abundance of the families Pseudomonadaceae and Enterobacteriaceae, as well as of the genus Acinetobacter were observed and positively correlated with plate count results (p
Vinicius Da Silva Duarte - One of the best experts on this subject based on the ideXlab platform.
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comparative evaluation of cheese whey microbial composition from four italian cheese factories by viable counts and 16s rrna gene amplicon sequencing
International Dairy Journal, 2020Co-Authors: Vinicius Da Silva Duarte, Milena Carlot, Shadi Pakroo, Armin Tarrah, Angiolella Lombardi, Helena Santiago, Viviana Corich, Alessio GiacominiAbstract:Abstract The microbiota of cheese whey from four dairy companies located in the North-East of Italy was evaluated during winter and spring using both culture-dependent and culture-independent methods. Although no significant differences were observed (p > 0.05) for any of the six microbial categories analysed by plate counting among the dairy companies, significant differences at the family and genus level were revealed by linear discriminant analysis (LDA). In particular, variations in the abundance of the families Pseudomonadaceae and Enterobacteriaceae, as well as of the genus Acinetobacter were observed and positively correlated with plate count results (p
Xavier F Malcata - One of the best experts on this subject based on the ideXlab platform.
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microbiological profile of maize and rye flours and sourdough used for the manufacture of traditional portuguese bread
Food Microbiology, 2012Co-Authors: Joao M Rocha, Xavier F MalcataAbstract:Abstract A thorough microbiological study of maize and rye flours, and sourdoughs obtained therefrom for eventual manufacture of broa – a dark sour bread typical in Northern Portugal, following artisanal practices, was carried out. Towards this purpose, samples were supplied by 14 artisanal producers, selected from 4 sub-regions, during two periods of the year. Total viable counts, as well as viable mesophilic and thermophilic microorganisms, yeasts and molds, Gram − rods, endospore-forming and nonsporing Gram + rods, and catalase + and catalase − Gram + cocci were assayed for. The comprehensive experimental dataset unfolded a unique and rather complex wild microflora in flours and sourdoughs throughout the whole region, which did not discriminate among sub-regions or seasons, or flour source for that matter. However, fermentation played a major role upon the numbers of the various microbial groups: the viable counts of yeasts, lactobacilli, streptococci, lactococci, enterococci and leuconostocs increased, whereas those of molds, Enterobacteriaceae, Pseudomonadaceae, staphylococci and micrococci decreased.
Yuan Tang - One of the best experts on this subject based on the ideXlab platform.
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Effect of biochar on the bioavailability of difenoconazole and microbial community composition in a pesticide-contaminated soil
Applied Soil Ecology, 2017Co-Authors: Jianzhong Cheng, Weichang Gao, Wenjie Pan, Xinqing Lee, Yi Chen, Yuan TangAbstract:Biochar is a soil amendment for carbon sequestration and contaminant remediation. However, there is relatively little information on the impact of biochar on soil microorganisms that play key roles in pollutant degradation. We investigated the interrelations between soil chemical properties, microbial community, and difenoconazole bioavailability after biochar amendments. Biochar was prepared by pyrolysis of flue-cured tobacco stalks. It was applied at four levels, 0%, 1.0%, 2.5%, and 5.0% (w/w), respectively, to soils in the presence and absence of tobacco plants (K326). Biochar increased soil pH, organic matter, total carbon, total nitrogen, C/N ratio and plant biomass. After 90 d incubation, 99% of the difenoconazole was degraded in planted and unplanted soils (control), whereas only 88% and 83% of difenoconazole was degraded in the 5.0% biochar-amended soil with and without plant, respectively. Difenoconazole had greater persistence in soil amended with biochar but plant uptake of difenoconazole was significantly decreased with increasing biochar application level. Compared to the control, the total plant residue of difenoconazole decreased by 24%, 41% and 45% in the 1.0%, 2.5% and 5.0% biochar treatments, respectively. Reduced plant uptake was related to increased sorption and microbial degradation of difenoconazole in biochar treated soils. High-throughput sequencing revealed that biochar altered soil bacterial community composition. Biochar amendment in planted soils increased the average relative abundance of Sphingomonadaceae and Pseudomonadaceae by 18% and 63%, respectively. When plants were absent, Sphingomonadaceae and Pseudomonadaceae increased by 46% and 110%, respectively. Therefore, biochar amendment enhances difenoconazole-degrading bacteria by modifying soil chemical properties, and eventually reduces difenoconazole bioavailability in contaminated soils.
Otto Holst - One of the best experts on this subject based on the ideXlab platform.
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a novel core region lacking heptose and phosphate of the lipopolysaccharide from the gram negative bacterium pseudomonas cichorii Pseudomonadaceae rna group 1
European Journal of Organic Chemistry, 2004Co-Authors: Cristina De Castro, Antonio Molinaro, Rosa Lanzetta, Michelangelo Parrilli, Rosa Nunziata, Otto HolstAbstract:The extracted lipopolysaccharides (LPSs) fraction of the plant-pathogenic bacterium Pseudomonascichorii contained two different molecules, the first of them an LPS possessing an O-specific polysaccharide made up of four 2-acetamido-2,6-dideoxy sugars linked linearly in a tetrasaccharide repeating unit. The structure of the second LPS component is reported here: it was of the rough form and contained a novel type of core region. The rough form LPS of Ps. cichorii was de-O-acylated by mild hydrazinolysis and then de-N-acylated with hot 4 M KOH. The oligosaccharide representing the complete carbohydrate backbone of the LPS was isolated by high-performance anion-exchange chromatography. Its structural characterization employed compositional and methylation analyses, matrix-assisted laser desorption/ionisation mass spectrometry and 1H, 13C and 31P NMR spectroscopy, with application of various 1D and 2D experiments. The carbohydrate backbone of this core region consisted of a hexasaccharide that contained no heptoses, being made up of L-Rhap, D-GlcpN and D-GalpN, besides the characteristic α-(24)-linked 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) disaccharide. The first Kdo residue linking the core region to the lipid A was substituted by a GalpN residue. This core region thus represents one of the rather few cases in which this position is not substituted by a manno-configured sugar. The structure of the LPS carbohydrate backbone is shown in Scheme 1. If not stated otherwise, sugars were D-configured pyranoses. Kdo is 3-deoxy-D-manno-oct-2-ulosonic acid. The lipid A backbone is N- and O-acylated in the LPSs. − Molecular modelling studies of the LPS carbohydrate backbone and of some of its parts were performed, yielding a preferred conformation that was surprisingly inflexible between the two Kdo residues. (© Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2004)
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A NOVEL CORE STRUCTURE, LACKING HEPTOSE AND PHOSPHATE, OF THE LIPOPOLYSACCHARIDE FROM THE GRAM-NEGATIVE BACTERIUM PSEUDOMONS CICHORII (Pseudomonadaceae RNA GROUP 1)
'Wiley', 2004Co-Authors: Cristina De Castro, Antonio Molinaro, Rosa Lanzetta, Michelangelo Parrilli, Rosa Nunziata, Otto HolstAbstract:The extracted lipopolysaccharides (LPSs) fraction of the plant-pathogenic bacterium Pseudomonas cichorii contained two different molecules, the first of them an LPS possessing an O-specific polysaccharide made up of four 2-acetamido-2,6-dideoxy sugars linked linearly in a tetrasaccharide repeating unit. The structure of the second LPS component is reported here: it was of the rough form and contained a novel type of core region. The rough form LPS of Ps. cichorii was de-O-acylated by mild hydrazinolysis and then de-N-acylated with hot 4 M KOH. The oligosaccharide representing the complete carbohydrate backbone of the LPS was isolated by high-performance anion-exchange chromatography. Its structural characterization employed compositional and methylation analyses, matrix-assisted laser desorption/ionisation mass spectrometry and 1H, 13C and 31P NMR spectroscopy, with application of various 1D and 2D experiments. The carbohydrate backbone of this core region consisted of a hexasaccharide that contained no heptoses, being made up of L-Rhap, D-GlcpN and D-GalpN, besides the characteristic α-(24)-linked 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) disaccharide. The first Kdo residue linking the core region to the lipid A was substituted by a GalpN residue. This core region thus represents one of the rather few cases in which this position is not substituted by a manno-configured sugar. The structure of the LPS carbohydrate backbone is shown in Scheme 1. If not stated otherwise, sugars were D-configured pyranoses. Kdo is 3-deoxy-D-manno-oct-2-ulosonic acid. The lipid A backbone is N- and O-acylated in the LPSs. − Molecular modelling studies of the LPS carbohydrate backbone and of some of its parts were performed, yielding a preferred conformation that was surprisingly inflexible between the two Kdo residues
