The Experts below are selected from a list of 1911 Experts worldwide ranked by ideXlab platform
Kenji Ueda - One of the best experts on this subject based on the ideXlab platform.
-
Enrichment and characterization of a bacterial mixture capable of utilizing C-mannosyl tryptophan as a carbon source
Glycoconjugate Journal, 2018Co-Authors: Tanim J. Hossain, Kenji Ueda, Shino Manabe, Toshiya Iida, Saori Kosono, Akira Hosomi, Daishi Inoue, Tadashi SuzukiAbstract:C -Mannosylation, a protein-modification found in various eukaryotes, involves the attachment of a single mannose molecule to selected tryptophan residues of proteins. Since C -mannosyl tryptophan (CMW) was detected in human urine, it is generally thought that CMW is not catabolized inside our body and instead is excreted via the urine. This paper reports enrichment of a bacterial consortium from soil that degrades CMW. The bacteria grew in minimal medium supplemented with CMW as the carbon source. Interestingly, even after successive clonal picks of individual colonies, several species were still present in each colony as revealed by 16S rRNA gene sequence analysis, indicating that a single species may not be responsible for this activity. A next generation sequencing (NGS) analysis was therefore carried out in order to determine which bacteria were responsible for the catabolism of CMW. It was found that a species of Sphingomonadaceae family, but not others, increased with simultaneous decrease of CMW in the media, suggesting that this species is most likely the one that is actively involved in the degradation of CMW.
-
Sphingomonas jaspsi sp. nov., a novel carotenoid-producing bacterium isolated from Misasa, Tottori, Japan.
International Journal of Systematic and Evolutionary Microbiology, 2007Co-Authors: Dalal Asker, Teruhiko Beppu, Kenji UedaAbstract:A yellow-pigmented, Gram-negative, motile, strictly aerobic, pleomorphic bacterium (strain TDMA-16T) was isolated from a freshwater sample collected at Misasa (Tottori, Japan). Strain TDMA-16T was slightly tolerant to gamma-ray irradiation and produced carotenoids, including zeaxanthin, nostoxanthin and an unknown carotenoid, effectively [1.7 mg (g dry cells)−1]. The DNA G+C content of strain TDMA-16T was 63.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences placed strain TDMA-16T in a distinct lineage in the family Sphingomonadaceae; sequence data showed that strain TDMA-16T was most closely related to Sphingomonas mali IFO 15500T (95.1 %), Sphingomonas aquatilis JSS7T (95.0 %), Sphingomonas pruni IFO 15498T (94.9 %), Sphingomonas melonis DSM 14444T (94.9 %) and Sphingomonas asaccharolytica IFO 15499T (94.5 %). The major fatty acids of strain TDMA-16T were C17 : 1 ω6c (34.5 %) and C18 : 1 ω7c (29.3 %). The presence of Q-10 as the main ubiquinone, the Sphingomonadaceae-specific sphingoglycolipid in the polar lipid profile and 2-hydroxy fatty acids, plus the absence of 3-hydroxy fatty acids, supported identification of this strain as a member of the genus Sphingomonas sensu stricto. Phylogenetic distinctiveness and unique phenotypic characteristics differentiated strain TDMA-16T from closely related Sphingomonas species. The results of polyphasic taxonomic analyses suggest that strain TDMA-16T represents a novel Sphingomonas species, for which the name Sphingomonas jaspsi sp. nov. is proposed. The type strain is strain TDMA-16T (=NBRC 102120T=DSM 18422T=CCUG 53607T).
-
Sphingomonas astaxanthinifaciens sp. nov., a novel astaxanthin-producing bacterium of the family Sphingomonadaceae isolated from Misasa, Tottori, Japan.
FEMS microbiology letters, 2007Co-Authors: Dalal Asker, Teruhiko Beppu, Kenji UedaAbstract:A red-pigmented, Gram-negative, motile, strictly aerobic, mesophilic, oval- or short rod-shaped bacterium (TDMA-17T) was isolated from fresh water collected at Misasa, a radioactive site in Japan. TDMA-17T was slightly tolerant against gamma-ray irradiation, and effectively produced carotenoids (2.8 mg g−1 dry cells) including, astaxanthin and astaxanthin isomers. Phylogenetic analysis based on 16S rRNA gene sequences placed TDMA-17T in a distinct lineage in the family Sphingomonadaceae, and the highest degree of sequence similarity determined were to Sphingomonas aerolata NW12T (94.5%), Sphingomonas aurantiaca MA101bT (94.0%), Sphingomonas melonis DAPP-PG 224T (94.0%), Sphingomonas asaccharolytica IFO 15499T (93.9%) and Sphingomonas abaci C42T (93.9%). The major fatty acids were C17 : 1ω6c (33.0%) and C18 : 1ω7c (20.8%). The DNA G+C content was 67.7 mol%. The presence of Q-10 as the main ubiquinone, the presence of Sphingomonadaceae-specific sphingoglycolipid in the polar lipid profiles, the presence of 2-hydroxy fatty acids and the absence of 3-hydroxy fatty acids supported the identification of this strain as a member of the genus Sphingomonas sensu stricto. Phylogenetic distinctiveness and unique phenotypic characteristics differentiated strain TDMA-17T from the closely related Sphingomonas species. The results of polyphasic taxonomic analyses suggested that TDMA-17T represents a novel Sphingomonas species, for which the name Sphingomonas astaxanthinifaciens sp. nov. is proposed. The type strain is TDMA-17T (=NBRC 102146=CCUG 53608).
-
Sphingomonas jaspsi sp. nov., a novel carotenoid-producing bacterium isolated from Misasa, Tottori, Japan.
International journal of systematic and evolutionary microbiology, 2007Co-Authors: Dalal Asker, Teruhiko Beppu, Kenji UedaAbstract:A yellow-pigmented, Gram-negative, motile, strictly aerobic, pleomorphic bacterium (strain TDMA-16(T)) was isolated from a freshwater sample collected at Misasa (Tottori, Japan). Strain TDMA-16(T) was slightly tolerant to gamma-ray irradiation and produced carotenoids, including zeaxanthin, nostoxanthin and an unknown carotenoid, effectively [1.7 mg (g dry cells)(-1)]. The DNA G+C content of strain TDMA-16(T) was 63.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences placed strain TDMA-16(T) in a distinct lineage in the family Sphingomonadaceae; sequence data showed that strain TDMA-16(T) was most closely related to Sphingomonas mali IFO 15500(T) (95.1 %), Sphingomonas aquatilis JSS7(T) (95.0 %), Sphingomonas pruni IFO 15498(T) (94.9 %), Sphingomonas melonis DSM 14444(T) (94.9 %) and Sphingomonas asaccharolytica IFO 15499(T) (94.5 %). The major fatty acids of strain TDMA-16(T) were C(17 : 1) omega 6c (34.5 %) and C(18 : 1) omega 7c (29.3 %). The presence of Q-10 as the main ubiquinone, the Sphingomonadaceae-specific sphingoglycolipid in the polar lipid profile and 2-hydroxy fatty acids, plus the absence of 3-hydroxy fatty acids, supported identification of this strain as a member of the genus Sphingomonas sensu stricto. Phylogenetic distinctiveness and unique phenotypic characteristics differentiated strain TDMA-16(T) from closely related Sphingomonas species. The results of polyphasic taxonomic analyses suggest that strain TDMA-16(T) represents a novel Sphingomonas species, for which the name Sphingomonas jaspsi sp. nov. is proposed. The type strain is strain TDMA-16(T) (=NBRC 102120(T)=DSM 18422(T)=CCUG 53607(T)).
Célia M Manaia - One of the best experts on this subject based on the ideXlab platform.
-
Genotypic diversity and antibiotic resistance in Sphingomonadaceae isolated from hospital tap water.
The Science of the total environment, 2013Co-Authors: Carlos Narciso-da-rocha, Ivone Vaz-moreira, Célia M ManaiaAbstract:The aim of this study was to infer about the modes and extent of dispersion of Sphingomonadaceae via tap water. Sphingomonadaceae isolated from tap water samples in different places of a hospital were compared, based on intra-species genetic variability and antibiotic resistance phenotypes. These isolates were also compared with others isolated before from houses and dental chairs, served by the same municipal water supply system. Sphingomonadaceae from hospital tap water comprised members of the genera Sphingomonas, Sphingobium, Novosphingobium and Blastomonas. In general, distinct genotypes of Sphingomonadaceae were detected in different hospital areas and in tap water outside the hospital, suggesting these bacteria are not persistent or widespread in the urban water distribution system. Possible intrinsic antibiotic resistance, observed in most or all members of the family or of a genus, was observed for colistin in Sphingomonadaceae, aminoglycosides in the genus Blastomonas and beta-lactams in the genus Sphingobium. Possible acquired resistance phenotypes, not common to all members of a given species, comprised fluoroquinolones, cephalosporins and sulphonamides. Although the potential of Sphingomonadaceae as opportunistic pathogens may be low, the capacity of these bacteria to thrive in water supply systems, combined with the intrinsic or acquired antibiotic resistance, may raise the risk associated with their occurrence in hospital tap water.
-
diversity and antibiotic resistance patterns of Sphingomonadaceae isolates from drinking water
Applied and Environmental Microbiology, 2011Co-Authors: Ivone Vazmoreira, Olga C Nunes, Célia M ManaiaAbstract:Sphingomonadaceae (n = 86) were isolated from a drinking water treatment plant (n = 6), tap water (n = 55), cup fillers for dental chairs (n = 21), and a water demineralization filter (n = 4). The bacterial isolates were identified based on analysis of the 16S rRNA gene sequence, and intraspecies variation was assessed on the basis of atpD gene sequence analysis. The isolates were identified as members of the genera Sphingomonas (n = 27), Sphingobium (n = 28), Novosphingobium (n = 12), Sphingopyxis (n = 7), and Blastomonas (n = 12). The patterns of susceptibility to five classes of antibiotics were analyzed and compared for the different sites of isolation and taxonomic groups. Colistin resistance was observed to be intrinsic (92%). The highest antibiotic resistance prevalence values were observed in members of the genera Sphingomonas and Sphingobium and for beta-lactams, ciprofloxacin, and cotrimoxazole. In tap water and in water from dental chairs, antibiotic resistance was more prevalent than in the other samples, mainly due to the predominance of isolates of the genera Sphingomonas and Sphingobium. These two genera presented distinct patterns of association with antibiotic resistance, suggesting different paths of resistance development. Antibiotic resistance patterns were often related to the species rather than to the site or strain, suggesting the importance of vertical resistance transmission in these bacteria. This is the first study demonstrating that members of the family Sphingomonadaceae are potential reservoirs of antibiotic resistance in drinking water.
-
Sphingobium vermicomposti sp. nov., isolated from vermicompost.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2009Co-Authors: Ivone Vaz-moreira, Olga C Nunes, Cátia Faria, Ana R. Lopes, Liselott A. Svensson, Enevold Falsen, Edward R. B. Moore, António César Silva Ferreira, Célia M ManaiaAbstract:Strain VC-230T was isolated from homemade vermicompost produced from kitchen waste. The isolate was a Gram-negative-staining, catalase- and oxidase-positive, motile rod-shaped bacterium able to grow at 15–37 °C and pH 6–8. On the basis of 16S rRNA gene sequence analysis, strain VC-230T was determined to belong to the family Sphingomonadaceae by its clustering with type strains of the genus Sphingobium, with Sphingobium chlorophenolicum ATCC 33790T (97.7 %) and Sphingobium herbicidovorans DSM 11019T (97.4 %) as its closest neighbours. The polar lipid pattern, the presence of spermidine and ubiquinone 10, the predominance of the cellular fatty acids C18 : 1 ω7c/9t/12t, C16 : 1 ω7c and C16 : 0 and the G+C content of the genomic DNA supported the affiliation of this organism to the genus Sphingobium. The phylogenetic, chemotaxonomic, phenotypic and DNA–DNA hybridization analyses verify that strain VC-230T represents a novel species, for which the name Sphingobium vermicomposti sp. nov. is proposed. The type strain is VC-230T (=CCUG 55809T =DSM 21299T).
Dalal Asker - One of the best experts on this subject based on the ideXlab platform.
-
Sphingomonas jaspsi sp. nov., a novel carotenoid-producing bacterium isolated from Misasa, Tottori, Japan.
International Journal of Systematic and Evolutionary Microbiology, 2007Co-Authors: Dalal Asker, Teruhiko Beppu, Kenji UedaAbstract:A yellow-pigmented, Gram-negative, motile, strictly aerobic, pleomorphic bacterium (strain TDMA-16T) was isolated from a freshwater sample collected at Misasa (Tottori, Japan). Strain TDMA-16T was slightly tolerant to gamma-ray irradiation and produced carotenoids, including zeaxanthin, nostoxanthin and an unknown carotenoid, effectively [1.7 mg (g dry cells)−1]. The DNA G+C content of strain TDMA-16T was 63.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences placed strain TDMA-16T in a distinct lineage in the family Sphingomonadaceae; sequence data showed that strain TDMA-16T was most closely related to Sphingomonas mali IFO 15500T (95.1 %), Sphingomonas aquatilis JSS7T (95.0 %), Sphingomonas pruni IFO 15498T (94.9 %), Sphingomonas melonis DSM 14444T (94.9 %) and Sphingomonas asaccharolytica IFO 15499T (94.5 %). The major fatty acids of strain TDMA-16T were C17 : 1 ω6c (34.5 %) and C18 : 1 ω7c (29.3 %). The presence of Q-10 as the main ubiquinone, the Sphingomonadaceae-specific sphingoglycolipid in the polar lipid profile and 2-hydroxy fatty acids, plus the absence of 3-hydroxy fatty acids, supported identification of this strain as a member of the genus Sphingomonas sensu stricto. Phylogenetic distinctiveness and unique phenotypic characteristics differentiated strain TDMA-16T from closely related Sphingomonas species. The results of polyphasic taxonomic analyses suggest that strain TDMA-16T represents a novel Sphingomonas species, for which the name Sphingomonas jaspsi sp. nov. is proposed. The type strain is strain TDMA-16T (=NBRC 102120T=DSM 18422T=CCUG 53607T).
-
Sphingomonas astaxanthinifaciens sp. nov., a novel astaxanthin-producing bacterium of the family Sphingomonadaceae isolated from Misasa, Tottori, Japan.
FEMS microbiology letters, 2007Co-Authors: Dalal Asker, Teruhiko Beppu, Kenji UedaAbstract:A red-pigmented, Gram-negative, motile, strictly aerobic, mesophilic, oval- or short rod-shaped bacterium (TDMA-17T) was isolated from fresh water collected at Misasa, a radioactive site in Japan. TDMA-17T was slightly tolerant against gamma-ray irradiation, and effectively produced carotenoids (2.8 mg g−1 dry cells) including, astaxanthin and astaxanthin isomers. Phylogenetic analysis based on 16S rRNA gene sequences placed TDMA-17T in a distinct lineage in the family Sphingomonadaceae, and the highest degree of sequence similarity determined were to Sphingomonas aerolata NW12T (94.5%), Sphingomonas aurantiaca MA101bT (94.0%), Sphingomonas melonis DAPP-PG 224T (94.0%), Sphingomonas asaccharolytica IFO 15499T (93.9%) and Sphingomonas abaci C42T (93.9%). The major fatty acids were C17 : 1ω6c (33.0%) and C18 : 1ω7c (20.8%). The DNA G+C content was 67.7 mol%. The presence of Q-10 as the main ubiquinone, the presence of Sphingomonadaceae-specific sphingoglycolipid in the polar lipid profiles, the presence of 2-hydroxy fatty acids and the absence of 3-hydroxy fatty acids supported the identification of this strain as a member of the genus Sphingomonas sensu stricto. Phylogenetic distinctiveness and unique phenotypic characteristics differentiated strain TDMA-17T from the closely related Sphingomonas species. The results of polyphasic taxonomic analyses suggested that TDMA-17T represents a novel Sphingomonas species, for which the name Sphingomonas astaxanthinifaciens sp. nov. is proposed. The type strain is TDMA-17T (=NBRC 102146=CCUG 53608).
-
Sphingomonas jaspsi sp. nov., a novel carotenoid-producing bacterium isolated from Misasa, Tottori, Japan.
International journal of systematic and evolutionary microbiology, 2007Co-Authors: Dalal Asker, Teruhiko Beppu, Kenji UedaAbstract:A yellow-pigmented, Gram-negative, motile, strictly aerobic, pleomorphic bacterium (strain TDMA-16(T)) was isolated from a freshwater sample collected at Misasa (Tottori, Japan). Strain TDMA-16(T) was slightly tolerant to gamma-ray irradiation and produced carotenoids, including zeaxanthin, nostoxanthin and an unknown carotenoid, effectively [1.7 mg (g dry cells)(-1)]. The DNA G+C content of strain TDMA-16(T) was 63.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences placed strain TDMA-16(T) in a distinct lineage in the family Sphingomonadaceae; sequence data showed that strain TDMA-16(T) was most closely related to Sphingomonas mali IFO 15500(T) (95.1 %), Sphingomonas aquatilis JSS7(T) (95.0 %), Sphingomonas pruni IFO 15498(T) (94.9 %), Sphingomonas melonis DSM 14444(T) (94.9 %) and Sphingomonas asaccharolytica IFO 15499(T) (94.5 %). The major fatty acids of strain TDMA-16(T) were C(17 : 1) omega 6c (34.5 %) and C(18 : 1) omega 7c (29.3 %). The presence of Q-10 as the main ubiquinone, the Sphingomonadaceae-specific sphingoglycolipid in the polar lipid profile and 2-hydroxy fatty acids, plus the absence of 3-hydroxy fatty acids, supported identification of this strain as a member of the genus Sphingomonas sensu stricto. Phylogenetic distinctiveness and unique phenotypic characteristics differentiated strain TDMA-16(T) from closely related Sphingomonas species. The results of polyphasic taxonomic analyses suggest that strain TDMA-16(T) represents a novel Sphingomonas species, for which the name Sphingomonas jaspsi sp. nov. is proposed. The type strain is strain TDMA-16(T) (=NBRC 102120(T)=DSM 18422(T)=CCUG 53607(T)).
Nianzhi Jiao - One of the best experts on this subject based on the ideXlab platform.
-
A Virus Infecting Marine Photoheterotrophic Alphaproteobacteria (Citromicrobium spp.) Defines a New Lineage of ssDNA Viruses.
Frontiers in microbiology, 2018Co-Authors: Qiang Zheng, Qi Chen, Curtis A. Suttle, Nianzhi JiaoAbstract:In recent metagenomic studies, single-stranded DNA (ssDNA) viruses that infect bacteria have been shown to be diverse and prevalent in the ocean; however, there are few isolates of marine ssDNA phages. Here, we report on a cultivated ssDNA phage (vB_Cib_ssDNA_P1) that infects Citromicrobium bathyomarinum RCC1878 (family Sphingomonadaceae), and other members of the genus. This is the first ssDNA phage reported to infect marine alphaproteobacteria, and represents a newly recognized lineage of the Microviridae infecting members of Sphingomonadaceae, the Amoyvirinae. The ~26 nm diameter polyhedral capsid contains a 4,360 bp genome with 6 open reading frames (ORFs) and a 59.3% G+C content. ORF1 encodes the capsid protein and ORF3 encodes the replication initiator protein. The replication cycle is ~5 hours, followed by a burst releasing about 180 infectious particles. The closest relative of vB_Cib_ssDNA_P1 is a prophage within the genome of Novosphingobium tardaugens strain NBRC16725. Phylogenetic analysis indicates that the vB_Cib_ssDNA_P1 phage and two related prophages, as well as an environmental sequence, form a novel group within the Microviridae. Our results indicate that this is a previously unknown lineage of ssDNA viruses which also supplies a new model system for studying interactions between ssDNA phages and marine bacteria.
-
Image_2_A Virus Infecting Marine Photoheterotrophic Alphaproteobacteria (Citromicrobium spp.) Defines a New Lineage of ssDNA Viruses.TIF
2018Co-Authors: Qiang Zheng, Qi Chen, Curtis A. Suttle, Nianzhi JiaoAbstract:In recent metagenomic studies, single-stranded DNA (ssDNA) viruses that infect bacteria have been shown to be diverse and prevalent in the ocean; however, there are few isolates of marine ssDNA phages. Here, we report on a cultivated ssDNA phage (vB_Cib_ssDNA_P1) that infects Citromicrobium bathyomarinum RCC1878 (family Sphingomonadaceae), and other members of the genus. This is the first ssDNA phage reported to infect marine alphaproteobacteria, and represents a newly recognized lineage of the Microviridae infecting members of Sphingomonadaceae, the Amoyvirinae. The ∼26 nm diameter polyhedral capsid contains a 4,360 bp genome with 6 open reading frames (ORFs) and a 59.3% G+C content. ORF1 encodes the capsid protein and ORF3 encodes the replication initiator protein. The replication cycle is ∼5 h, followed by a burst releasing about 180 infectious particles. The closest relative of vB_Cib_ssDNA_P1 is a prophage within the genome of Novosphingobium tardaugens strain NBRC16725. Phylogenetic analysis indicates that the vB_Cib_ssDNA_P1 phage and two related prophages, as well as an environmental sequence, form a novel group within the Microviridae. Our results indicate that this is a previously unknown lineage of ssDNA viruses which also supplies a new model system for studying interactions between ssDNA phages and marine bacteria.
-
Binariimonas pacifica gen. nov., sp. nov., a Novel Marine Bacterium of Family Sphingomonadaceae Isolated from East Pacific Ocean Surface Seawater.
Current microbiology, 2015Co-Authors: Zhao Zhao, Jia Sun, Rui Zhang, Nianzhi JiaoAbstract:A novel rod-shaped binary fission, and yellow-pigmented bacterial strain, JLT 2480T, was isolated from surface seawater in the East Pacific Ocean. The strain is Gram negative and oxidase negative. Phylogenetic analyses based on 16S rRNA gene sequence indicate that strain JLT 2480T falls in the family Sphingomonadaceae, sharing highest similarity (95.6 %) with the species Blastomonas ursincola. The DNA G+C content of JLT 2480T is 65.5 mol%, and the sole respiratory quinone is coenzyme Q10. The predominant polar lipids are sphingoglycolipids (SGL1 and SGL2), phosphatidylglycerols, phosphatidylethanolamines, phospholipids, glycolipids, and phosphatidylcholines. The predominant cellular fatty acids are C16:0, C18:0, C18:1ω7c, C12:0, and C16:1ω7c. Strain JLT 2480T is distinct from the B. ursincola type strain DSM 9006T as reflected by major chemotaxonomic distinctions between the two. Furthermore, two notable characteristics of the genus Blastomonas, that is, the presence of bacteriochlorophyll a and the puf genes, are not detected in JLT 2480T. On the basis of present evidence, we consider JLT 2480T to be a novel species in a new genus of the family Sphingomonadaceae, and propose the name Binariimonas pacifica gen. nov., sp. nov., with strain JLT 2480T (=CGMCC 1.12850T = DSM 28646T) to be the type strain for genus Binariimonas.
-
Pacificamonas flava gen nov, sp nov, a Novel Member of the Family Sphingomonadaceae Isolated from the Southeastern Pacific
Current microbiology, 2014Co-Authors: Keshao Liu, Nianzhi Jiao, Kai TangAbstract:Strain JLT2015T was isolated from surface seawater of the Southeastern Pacific. The strain was Gram-negative, aerobic, motile by gliding, and rod shaped. The dominant fatty acids were C18:1ω7c, C16:0, and C16:1ω7c. The major respiratory ubiquinone was Q-10, and the predominant polyamine pattern was spermidine. The components of the polar lipid profile were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, and sphingoglycolipid. The DNA G+C content was 64.2 %. Phylogenetic analysis based on 16S rRNA gene sequence revealed strain JLT2015T belonged to belong to the family Sphingomonadaceae, exhibiting 94.7 % 16S rRNA gene sequence similarity with Novosphingobium pentaromativorans. On the basis of the taxonomic data presented, together with phylogenetic and genetic characteristics, strain JLT2015T is considered to represent a novel genus, for which the name Pacificamonas flava gen. nov., sp. nov. is proposed. The type strain of Pacificamonas flava is JLT2015T (=LMG27364T = CGMCC1.12401T).
-
Draft Genome Sequence of Strain JLT2015T, Belonging to the Family Sphingomonadaceae of the Alphaproteobacteria.
Genome announcements, 2013Co-Authors: Kai Tang, Kesao Liu, Nianzhi JiaoAbstract:Strain JLT2015(T) was isolated from the southeastern Pacific, as a representative of a new genus of the family Sphingomonadaceae of the Alphaproteobacteria. Here, we present the draft genome sequence of strain JLT2015(T), which provides insight into the oligotrophic strategy of this organism.
Teruhiko Beppu - One of the best experts on this subject based on the ideXlab platform.
-
Sphingomonas jaspsi sp. nov., a novel carotenoid-producing bacterium isolated from Misasa, Tottori, Japan.
International Journal of Systematic and Evolutionary Microbiology, 2007Co-Authors: Dalal Asker, Teruhiko Beppu, Kenji UedaAbstract:A yellow-pigmented, Gram-negative, motile, strictly aerobic, pleomorphic bacterium (strain TDMA-16T) was isolated from a freshwater sample collected at Misasa (Tottori, Japan). Strain TDMA-16T was slightly tolerant to gamma-ray irradiation and produced carotenoids, including zeaxanthin, nostoxanthin and an unknown carotenoid, effectively [1.7 mg (g dry cells)−1]. The DNA G+C content of strain TDMA-16T was 63.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences placed strain TDMA-16T in a distinct lineage in the family Sphingomonadaceae; sequence data showed that strain TDMA-16T was most closely related to Sphingomonas mali IFO 15500T (95.1 %), Sphingomonas aquatilis JSS7T (95.0 %), Sphingomonas pruni IFO 15498T (94.9 %), Sphingomonas melonis DSM 14444T (94.9 %) and Sphingomonas asaccharolytica IFO 15499T (94.5 %). The major fatty acids of strain TDMA-16T were C17 : 1 ω6c (34.5 %) and C18 : 1 ω7c (29.3 %). The presence of Q-10 as the main ubiquinone, the Sphingomonadaceae-specific sphingoglycolipid in the polar lipid profile and 2-hydroxy fatty acids, plus the absence of 3-hydroxy fatty acids, supported identification of this strain as a member of the genus Sphingomonas sensu stricto. Phylogenetic distinctiveness and unique phenotypic characteristics differentiated strain TDMA-16T from closely related Sphingomonas species. The results of polyphasic taxonomic analyses suggest that strain TDMA-16T represents a novel Sphingomonas species, for which the name Sphingomonas jaspsi sp. nov. is proposed. The type strain is strain TDMA-16T (=NBRC 102120T=DSM 18422T=CCUG 53607T).
-
Sphingomonas astaxanthinifaciens sp. nov., a novel astaxanthin-producing bacterium of the family Sphingomonadaceae isolated from Misasa, Tottori, Japan.
FEMS microbiology letters, 2007Co-Authors: Dalal Asker, Teruhiko Beppu, Kenji UedaAbstract:A red-pigmented, Gram-negative, motile, strictly aerobic, mesophilic, oval- or short rod-shaped bacterium (TDMA-17T) was isolated from fresh water collected at Misasa, a radioactive site in Japan. TDMA-17T was slightly tolerant against gamma-ray irradiation, and effectively produced carotenoids (2.8 mg g−1 dry cells) including, astaxanthin and astaxanthin isomers. Phylogenetic analysis based on 16S rRNA gene sequences placed TDMA-17T in a distinct lineage in the family Sphingomonadaceae, and the highest degree of sequence similarity determined were to Sphingomonas aerolata NW12T (94.5%), Sphingomonas aurantiaca MA101bT (94.0%), Sphingomonas melonis DAPP-PG 224T (94.0%), Sphingomonas asaccharolytica IFO 15499T (93.9%) and Sphingomonas abaci C42T (93.9%). The major fatty acids were C17 : 1ω6c (33.0%) and C18 : 1ω7c (20.8%). The DNA G+C content was 67.7 mol%. The presence of Q-10 as the main ubiquinone, the presence of Sphingomonadaceae-specific sphingoglycolipid in the polar lipid profiles, the presence of 2-hydroxy fatty acids and the absence of 3-hydroxy fatty acids supported the identification of this strain as a member of the genus Sphingomonas sensu stricto. Phylogenetic distinctiveness and unique phenotypic characteristics differentiated strain TDMA-17T from the closely related Sphingomonas species. The results of polyphasic taxonomic analyses suggested that TDMA-17T represents a novel Sphingomonas species, for which the name Sphingomonas astaxanthinifaciens sp. nov. is proposed. The type strain is TDMA-17T (=NBRC 102146=CCUG 53608).
-
Sphingomonas jaspsi sp. nov., a novel carotenoid-producing bacterium isolated from Misasa, Tottori, Japan.
International journal of systematic and evolutionary microbiology, 2007Co-Authors: Dalal Asker, Teruhiko Beppu, Kenji UedaAbstract:A yellow-pigmented, Gram-negative, motile, strictly aerobic, pleomorphic bacterium (strain TDMA-16(T)) was isolated from a freshwater sample collected at Misasa (Tottori, Japan). Strain TDMA-16(T) was slightly tolerant to gamma-ray irradiation and produced carotenoids, including zeaxanthin, nostoxanthin and an unknown carotenoid, effectively [1.7 mg (g dry cells)(-1)]. The DNA G+C content of strain TDMA-16(T) was 63.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences placed strain TDMA-16(T) in a distinct lineage in the family Sphingomonadaceae; sequence data showed that strain TDMA-16(T) was most closely related to Sphingomonas mali IFO 15500(T) (95.1 %), Sphingomonas aquatilis JSS7(T) (95.0 %), Sphingomonas pruni IFO 15498(T) (94.9 %), Sphingomonas melonis DSM 14444(T) (94.9 %) and Sphingomonas asaccharolytica IFO 15499(T) (94.5 %). The major fatty acids of strain TDMA-16(T) were C(17 : 1) omega 6c (34.5 %) and C(18 : 1) omega 7c (29.3 %). The presence of Q-10 as the main ubiquinone, the Sphingomonadaceae-specific sphingoglycolipid in the polar lipid profile and 2-hydroxy fatty acids, plus the absence of 3-hydroxy fatty acids, supported identification of this strain as a member of the genus Sphingomonas sensu stricto. Phylogenetic distinctiveness and unique phenotypic characteristics differentiated strain TDMA-16(T) from closely related Sphingomonas species. The results of polyphasic taxonomic analyses suggest that strain TDMA-16(T) represents a novel Sphingomonas species, for which the name Sphingomonas jaspsi sp. nov. is proposed. The type strain is strain TDMA-16(T) (=NBRC 102120(T)=DSM 18422(T)=CCUG 53607(T)).
