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C Y Liu - One of the best experts on this subject based on the ideXlab platform.
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comparison of aerobic denitrification under high oxygen atmosphere by thiosphaera pantotropha atcc 35512 and Pseudomonas stutzeri su2 newly isolated from the activated sludge of a piggery wastewater treatment system
Journal of Applied Microbiology, 2001Co-Authors: Y Liu, C Y LiuAbstract:AIMS: This study compares the ability of Thiosphaera pantotropha ATCC 35512 and the newly isolated Pseudomonas stutzeri SU2 to perform aerobic denitrification. METHODS AND RESULTS: Nitrate-supplemented basal medium in airtight crimp-sealed serum bottles containing an atmosphere of 92% oxygen was inoculated with Ps. stutzeri SU2 or T. pantotropha and incubated at 30 degrees C. During the 92-h incubation period, aerobic denitrification by Ps. stutzeri SU2 (NO3(-) - N removal 99.24%) was more efficient than that by T. pantotropha (NO3(-) - N removal 27.29%). CONCLUSION: Pseudomonas stutzeri SU2, which was isolated from the activated sludge of a sequencing batch reactor treating piggery wastewater, rapidly reduced the nitrate to nitrogen gas without nitrite accumulation. The nitrate removal rate of SU2 was 0.032 mmol NO3(-) - N g cell-1 h-1 after 44 h incubation. SIGNIFICANCE AND IMPACT OF THE STUDY: Pseudomonas stutzeri SU2 can be used in a full-scale sequencing batch system for efficient in situ aerobic nitrate removal from piggery wastewater.
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comparison of aerobic denitrification under high oxygen atmosphere by thiosphaera pantotropha atcc 35512 and Pseudomonas stutzeri su2 newly isolated from the activated sludge of a piggery wastewater treatment system
Journal of Applied Microbiology, 2001Co-Authors: Bin Liu, C Y LiuAbstract:Aims: This study compares the ability of Thiosphaera pantotropha ATCC 35512 and the newly isolated Pseudomonas stutzeri SU2 to perform aerobic denitrification. Methods and Results: Nitrate-supplemented basal medium in airtight crimp-sealed serum bottles containing an atmosphere of 92% oxygen was inoculated with Ps. stutzeri SU2 or T. pantotropha and incubated at 30 °C. During the 92-h incubation period, aerobic denitrification by Ps. stutzeri SU2 (NO3– – N removal 99·24%) was more efficient than that by T. pantotropha (NO3– – N removal 27·29%). Conclusions: Pseudomonas stutzeri SU2, which was isolated from the activated sludge of a sequencing batch reactor treating piggery wastewater, rapidly reduced the nitrate to nitrogen gas without nitrite accumulation. The nitrate removal rate of SU2 was 0·032 mmol NO3– – N g cell−1 h–1 after 44 h incubation. Significance and Impact of the Study: Pseudomonas stutzeri SU2 can be used in a full-scale sequencing batch system for efficient in situ aerobic nitrate removal from piggery wastewater.
Jorge Lalucat - One of the best experts on this subject based on the ideXlab platform.
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complete genome sequence of the naphthalene degrading bacterium Pseudomonas stutzeri an10 ccug 29243
Journal of Bacteriology, 2012Co-Authors: Isabel Brunetgalmes, Jorge Lalucat, Margarita Gomila, Elena Garciavaldes, Antonio Busquets, Arantxa Pena, Balbina Nogales, Antonio BennasarAbstract:Pseudomonas stutzeri AN10 (CCUG 29243) can be considered a model strain for aerobic naphthalene degradation. We report the complete genome sequence of this bacterium. Its 4.71-Mb chromosome provides insights into other biodegradative capabilities of strain AN10 (i.e., benzoate catabolism) and suggests a high number of horizontal gene transfer events.
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genome sequence of Pseudomonas stutzeri strain jm300 dsm 10701 a soil isolate and model organism for natural transformation
Journal of Bacteriology, 2012Co-Authors: Antonio Busquets, Rafael Bosch, Jorge Lalucat, Margarita Gomila, Elena Garciavaldes, Arantxa Pena, Balbina Nogales, Antonio BennasarAbstract:Pseudomonas stutzeri strain JM300 (DSM 10701) is a denitrifying soil isolate and a model organism for natural transformation in bacteria. Here we report the first complete genome sequence of JM300, the reference strain of genomovar 8 for the species.
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draft genome of Pseudomonas stutzeri strain zobell ccug 16156 a marine isolate and model organism for denitrification studies
Journal of Bacteriology, 2012Co-Authors: Arantxa Pena, Rafael Bosch, Jorge Lalucat, Margarita Gomila, Elena Garciavaldes, Antonio Busquets, Balbina Nogales, Antonio BennasarAbstract:Pseudomonas stutzeri strain ZoBell, formerly a strain of Pseudomonas perfectomarina (CCUG 16156 = ATCC 14405), is a model organism for denitrification. It was isolated by ZoBell in 1944 from a marine sample, and here we report the first genome draft of a strain assigned to genomovar 2 of the species P. stutzeri.
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phylogenetic analysis and siderotyping as useful tools in the taxonomy of Pseudomonas stutzeri description of a novel genomovar
International Journal of Systematic and Evolutionary Microbiology, 2008Co-Authors: Magdalena Mulet, Jeanmarie Meyer, Christelle Gruffaz, Norberto J Palleroni, Jorge Lalucat, Margarita Gomila, Elena GarciavaldesAbstract:An examination of the results of phylogenetic analyses based on the sequences of fragments of the 16S rRNA, gyrB and rpoD genes, and the discrimination of genomovars based on siderophore diversity within the genus Pseudomonas, has added important taxonomic tools in the characterization of Pseudomonas stutzeri. Eighteen reference strains, nine newly identified hydrocarbon-degrading strains and three strains showing relevant physiological characteristics of P. stutzeri, together with the type strains of four related species, were included in the study. A novel genomovar within the species is described. A summary of the methodology used in these studies and the results of our attempts to define a solid internal subdivision of this important species within the genus Pseudomonas are presented and discussed.
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ispst9 an isl3 like insertion sequence from Pseudomonas stutzeri an10 involved in catabolic gene inactivation
International Microbiology, 2008Co-Authors: Joseph Alexander Christieoleza, Jorge Lalucat, Balbina Nogales, Celia Martincardona, Mariana P Lanfranconi, Sebastian Alberti, Rafael BoschAbstract:A novel insertion sequence (IS), ISPst9, from Pseudomonas stutzeri AN10 was cloned and characterized. ISPst9 is a typical bacterial IS, consisting of a 2472-bp element flanked by 24-bp perfect inverted repeats that generates 8-bp AT-rich target duplications upon insertion. The sequence also contains a gene that encodes an active transposase (TnpA) with significant amino acid identity to members of the ISL3 family. Southern blot analysis of digested genomic DNA of strain AN10 and its 4-chlorosalicylate-degrading derivative strain AN142 demonstrated that native ISPst9 transposes in multiple copies, with one of them responsible for the nahH insertional inactivation observed in strain AN142. Precise excision of ISPst9 yielded NahH+ revertants of AN142 at high frequencies (up to 10E-6). In vivo transposition, mainly in multiple copies, of an ISPst9 derivative containing a KmR cassette cloned into a suicide vector was also demonstrated. Hybridization experiments carried out with different strains of P. stutzeri and with 292 phylogenetically distinct environmental isolates suggested that the presence of an ISPst9-like IS occurs in diverse bacteria together with the presence of aromatic hydrocarbon-degrading determinants.
Paola Barbieri - One of the best experts on this subject based on the ideXlab platform.
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an airlift biofilm reactor for the biodegradation of phenol by Pseudomonas stutzeri ox1
Journal of Biotechnology, 2006Co-Authors: Ambra Viggiani, Paola Barbieri, Giuseppe Olivieri, Loredana Siani, A Di Donato, Antonio Marzocchella, Piero Salatino, Enrica GalliAbstract:Phenol bioconversion by Pseudomonas stutzeri OX1 using either free or immobilized cells was investigated with the aim of searching for optimal operating conditions of a continuous bioconversion process. The study was developed by analyzing: (a) free-cell growth and products of phenol bioconversion by batch cultures of P. stutzeri; (b) growth of P. stutzeri cells immobilized on carrier particles; (c) bioconversion of phenol-bearing liquid streams and the establishment and growth of an active bacterial biofilm during continuous operation of an internal-loop airlift bioreactor. We have confirmed that free Pseudomonas cultures are able to transform phenol through the classical meta pathway for the degradation of aromatic molecules. Data indicate that bacterial growth is substrate-inhibited, with a limiting phenol concentration of about 600 mg/L. Immobilization tests revealed that a stable bacterial biofilm can be formed on various types of solid carriers (silica sand, tuff, and activated carbon), but not on alumina. Entrapment in alginate beads also proved to be effective for P. stutzeri immobilization. Continuous bioconversion of phenol-bearing liquid streams was successfully obtained in a biofilm reactor operated in the internal-circulation airlift mode. Phenol conversion exceeded 95%. Biofilm formation and growth during continuous operation of the airlift bioreactor were quantitatively and qualitatively assessed.
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organization and regulation of meta cleavage pathway genes for toluene and o xylene derivative degradation in Pseudomonas stutzeri ox1
Applied and Environmental Microbiology, 2001Co-Authors: Fabio L G Arenghi, Enrica Galli, Davide Berlanda, Guido Sello, Paola BarbieriAbstract:Pseudomonas stutzeri OX1 meta pathway genes for toluene and o-xylene catabolism were analyzed, and loci encoding phenol hydroxylase, catechol 2,3-dioxygenase, 2-hydroxymuconate semialdehyde dehydrogenase, and 2-hydroxymuconate semialdehyde hydrolase were mapped. Phenol hydroxylase converted a broad range of substrates, as it was also able to transform the nongrowth substrates 2,4-dimethylphenol and 2,5-dimethylphenol into 3,5-dimethylcatechol and 3,6-dimethylcatechol, respectively, which, however, were not cleaved by catechol 2,3-dioxygenase. The identified gene cluster displayed a gene order similar to that of the Pseudomonas sp. strain CF600 dmp operon for phenol catabolism and was found to be coregulated by the tou operon activator TouR. A hypothesis about the evolution of the toluene and o-xylene catabolic pathway in P. stutzeri OX1 is discussed.
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Aerobic degradation of tetrachloroethylene by toluene-o-xylene monooxygenase of Pseudomonas stutzeri OX1
Nature Biotechnology, 2000Co-Authors: Doohyun Ryoo, Hojae Shim, Keith Canada, Paola Barbieri, Thomas K. WoodAbstract:Tetrachloroethylene (PCE) is thought to have no natural source, so it is one of the most difficult contaminants to degrade biologically. This common groundwater pollutant was thought completely nonbiodegradable in the presence of oxygen. Here we report that the wastewater bacterium Pseudomonas stutzeri OX1 degrades aerobically 0.56 μmol of 2.0 μmol PCE in 21 h (V_max ≈ 2.5 nmol min^−1 mg^−1 protein and K_M ≈ 34 μM). These results were corroborated by the generation of 0.48 μmol of the degradation product, chloride ions. This degradation was confirmed to be a result of expression of toluene- o -xylene monooxygenase (ToMO) by P. stutzeri OX1, since cloning and expressing this enzyme in Escherichia coli led to the aerobic degradation of 0.19 μmol of 2.0 μmol PCE and the generation of stoichiometric amounts of chloride. In addition, PCE induces formation of ToMO, which leads to its own degradation in P. stutzeri OX1. Degradation intermediates reduce the growth rate of this strain by 27%.
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analysis of the gene cluster encoding toluene o xylene monooxygenase from Pseudomonas stutzeri ox1
Applied and Environmental Microbiology, 1998Co-Authors: Giovanni Bertoni, Manuela Martino, Enrica Galli, Paola BarbieriAbstract:The toluene/o-xylene monooxygenase cloned from Pseudomonas stutzeri OX1 displays a very broad range of substrates and a very peculiar regioselectivity, because it is able to hydroxylate more than one position on the aromatic ring of several hydrocarbons and phenols. The nucleotide sequence of the gene cluster coding for this enzymatic system has been determined. The sequence analysis revealed the presence of six open reading frames (ORFs) homologous to other genes clustered in operons coding for multicomponent monooxygenases found in benzene- and toluene-degradative pathways cloned from Pseudomonas strains. Significant similarities were also found with multicomponent monooxygenase systems for phenol, methane, alkene, and dimethyl sulfide cloned from different bacterial strains. The knockout of each ORF and complementation with the wild-type allele indicated that all six ORFs are essential for the full activity of the toluene/o-xylene monooxygenase in Escherichia coli. This analysis also shows that despite its activity on both hydrocarbons and phenols, toluene/ o-xylene monooxygenase belongs to a toluene multicomponent monooxygenase subfamily rather than to the monooxygenases active on phenols.
Elena Garciavaldes - One of the best experts on this subject based on the ideXlab platform.
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complete genome sequence of the naphthalene degrading bacterium Pseudomonas stutzeri an10 ccug 29243
Journal of Bacteriology, 2012Co-Authors: Isabel Brunetgalmes, Jorge Lalucat, Margarita Gomila, Elena Garciavaldes, Antonio Busquets, Arantxa Pena, Balbina Nogales, Antonio BennasarAbstract:Pseudomonas stutzeri AN10 (CCUG 29243) can be considered a model strain for aerobic naphthalene degradation. We report the complete genome sequence of this bacterium. Its 4.71-Mb chromosome provides insights into other biodegradative capabilities of strain AN10 (i.e., benzoate catabolism) and suggests a high number of horizontal gene transfer events.
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genome sequence of Pseudomonas stutzeri strain jm300 dsm 10701 a soil isolate and model organism for natural transformation
Journal of Bacteriology, 2012Co-Authors: Antonio Busquets, Rafael Bosch, Jorge Lalucat, Margarita Gomila, Elena Garciavaldes, Arantxa Pena, Balbina Nogales, Antonio BennasarAbstract:Pseudomonas stutzeri strain JM300 (DSM 10701) is a denitrifying soil isolate and a model organism for natural transformation in bacteria. Here we report the first complete genome sequence of JM300, the reference strain of genomovar 8 for the species.
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draft genome of Pseudomonas stutzeri strain zobell ccug 16156 a marine isolate and model organism for denitrification studies
Journal of Bacteriology, 2012Co-Authors: Arantxa Pena, Rafael Bosch, Jorge Lalucat, Margarita Gomila, Elena Garciavaldes, Antonio Busquets, Balbina Nogales, Antonio BennasarAbstract:Pseudomonas stutzeri strain ZoBell, formerly a strain of Pseudomonas perfectomarina (CCUG 16156 = ATCC 14405), is a model organism for denitrification. It was isolated by ZoBell in 1944 from a marine sample, and here we report the first genome draft of a strain assigned to genomovar 2 of the species P. stutzeri.
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phylogenetic analysis and siderotyping as useful tools in the taxonomy of Pseudomonas stutzeri description of a novel genomovar
International Journal of Systematic and Evolutionary Microbiology, 2008Co-Authors: Magdalena Mulet, Jeanmarie Meyer, Christelle Gruffaz, Norberto J Palleroni, Jorge Lalucat, Margarita Gomila, Elena GarciavaldesAbstract:An examination of the results of phylogenetic analyses based on the sequences of fragments of the 16S rRNA, gyrB and rpoD genes, and the discrimination of genomovars based on siderophore diversity within the genus Pseudomonas, has added important taxonomic tools in the characterization of Pseudomonas stutzeri. Eighteen reference strains, nine newly identified hydrocarbon-degrading strains and three strains showing relevant physiological characteristics of P. stutzeri, together with the type strains of four related species, were included in the study. A novel genomovar within the species is described. A summary of the methodology used in these studies and the results of our attempts to define a solid internal subdivision of this important species within the genus Pseudomonas are presented and discussed.
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biology of Pseudomonas stutzeri
Microbiology and Molecular Biology Reviews, 2006Co-Authors: Jorge Lalucat, Antonio Bennasar, Rafael Bosch, Elena Garciavaldes, Norberto J PalleroniAbstract:Pseudomonas stutzeri is a nonfluorescent denitrifying bacterium widely distributed in the environment, and it has also been isolated as an opportunistic pathogen from humans. Over the past 15 years, much progress has been made in elucidating the taxonomy of this diverse taxonomical group, demonstrating the clonality of its populations. The species has received much attention because of its particular metabolic properties: it has been proposed as a model organism for denitrification studies; many strains have natural transformation properties, making it relevant for study of the transfer of genes in the environment; several strains are able to fix dinitrogen; and others participate in the degradation of pollutants or interact with toxic metals. This review considers the history of the discovery, nomenclatural changes, and early studies, together with the relevant biological and ecological properties, of P. stutzeri.
Antonio Bennasar - One of the best experts on this subject based on the ideXlab platform.
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complete genome sequence of the naphthalene degrading bacterium Pseudomonas stutzeri an10 ccug 29243
Journal of Bacteriology, 2012Co-Authors: Isabel Brunetgalmes, Jorge Lalucat, Margarita Gomila, Elena Garciavaldes, Antonio Busquets, Arantxa Pena, Balbina Nogales, Antonio BennasarAbstract:Pseudomonas stutzeri AN10 (CCUG 29243) can be considered a model strain for aerobic naphthalene degradation. We report the complete genome sequence of this bacterium. Its 4.71-Mb chromosome provides insights into other biodegradative capabilities of strain AN10 (i.e., benzoate catabolism) and suggests a high number of horizontal gene transfer events.
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genome sequence of Pseudomonas stutzeri strain jm300 dsm 10701 a soil isolate and model organism for natural transformation
Journal of Bacteriology, 2012Co-Authors: Antonio Busquets, Rafael Bosch, Jorge Lalucat, Margarita Gomila, Elena Garciavaldes, Arantxa Pena, Balbina Nogales, Antonio BennasarAbstract:Pseudomonas stutzeri strain JM300 (DSM 10701) is a denitrifying soil isolate and a model organism for natural transformation in bacteria. Here we report the first complete genome sequence of JM300, the reference strain of genomovar 8 for the species.
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draft genome of Pseudomonas stutzeri strain zobell ccug 16156 a marine isolate and model organism for denitrification studies
Journal of Bacteriology, 2012Co-Authors: Arantxa Pena, Rafael Bosch, Jorge Lalucat, Margarita Gomila, Elena Garciavaldes, Antonio Busquets, Balbina Nogales, Antonio BennasarAbstract:Pseudomonas stutzeri strain ZoBell, formerly a strain of Pseudomonas perfectomarina (CCUG 16156 = ATCC 14405), is a model organism for denitrification. It was isolated by ZoBell in 1944 from a marine sample, and here we report the first genome draft of a strain assigned to genomovar 2 of the species P. stutzeri.
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biology of Pseudomonas stutzeri
Microbiology and Molecular Biology Reviews, 2006Co-Authors: Jorge Lalucat, Antonio Bennasar, Rafael Bosch, Elena Garciavaldes, Norberto J PalleroniAbstract:Pseudomonas stutzeri is a nonfluorescent denitrifying bacterium widely distributed in the environment, and it has also been isolated as an opportunistic pathogen from humans. Over the past 15 years, much progress has been made in elucidating the taxonomy of this diverse taxonomical group, demonstrating the clonality of its populations. The species has received much attention because of its particular metabolic properties: it has been proposed as a model organism for denitrification studies; many strains have natural transformation properties, making it relevant for study of the transfer of genes in the environment; several strains are able to fix dinitrogen; and others participate in the degradation of pollutants or interact with toxic metals. This review considers the history of the discovery, nomenclatural changes, and early studies, together with the relevant biological and ecological properties, of P. stutzeri.
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Comparative Genetic Diversity of Pseudomonas stutzeri Genomovars, Clonal Structure, and Phylogeny of the Species
Journal of Bacteriology, 2004Co-Authors: Aina Maria Cladera, Antonio Bennasar, Maria Barceló, Jorge Lalucat, Elena García-valdésAbstract:A combined phylogenetic and multilocus DNA sequence analysis of 26 Pseudomonas stutzeri strains distributed within the 9 genomovars of the species has been performed. Type strains of the two most closely related species (P. balearica, former genomovar 6, and P. mendocina), together with P. aeruginosa, as the type species of the genus, have been included in the study. The extremely high genetic diversity and the clonal structure of the species were confirmed by the sequence analysis. Clustering of strains in the consensus phylogeny inferred from the analysis of seven nucleotide sequences (16S ribosomal DNA, internally transcribed spacer region 1, gyrB, rpoD, nosZ, catA, and nahH) confirmed the monophyletic origin of the genomovars within the Pseudomonas branch and is in good agreement with earlier DNA-DNA similarity analysis, indicating that the selected genes are representative of the whole genome in members of the species.
