The Experts below are selected from a list of 2022 Experts worldwide ranked by ideXlab platform
Carolin Huhn - One of the best experts on this subject based on the ideXlab platform.
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Nonaqueous capillary electrophoresis-mass spectrometry: a versatile, straightforward tool for the analysis of alkaloids from Psychoactive Plant extracts.
Electrophoresis, 2012Co-Authors: Tjorben Nils Posch, Nathalie Martin, Michael Pütz, Carolin HuhnAbstract:In this study we show that a nonaqueous capillary electrophoresis mass spectrometry (NACE-MS) method carefully optimized by a design of experiment can be applied to a very large number of alkaloids in different Plant extracts. It is possible to characterize the pattern of the Psychoactive alkaloids in several Plant samples and preparations thereof, each presenting different challenges in their analysis. The method is shown to be able to separate structurally closely related substances, diastereomers and further isobaric compounds, to separate members of different alkaloid classes within one run and to tolerate significant matrix load. A comparison with methods presented in the literature reveals that a near-generic NACE-MS method for the fast profiling of alkaloids in forensically relevant Plant samples has been developed.
Tjorben Nils Posch - One of the best experts on this subject based on the ideXlab platform.
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Nonaqueous capillary electrophoresis-mass spectrometry: a versatile, straightforward tool for the analysis of alkaloids from Psychoactive Plant extracts.
Electrophoresis, 2012Co-Authors: Tjorben Nils Posch, Nathalie Martin, Michael Pütz, Carolin HuhnAbstract:In this study we show that a nonaqueous capillary electrophoresis mass spectrometry (NACE-MS) method carefully optimized by a design of experiment can be applied to a very large number of alkaloids in different Plant extracts. It is possible to characterize the pattern of the Psychoactive alkaloids in several Plant samples and preparations thereof, each presenting different challenges in their analysis. The method is shown to be able to separate structurally closely related substances, diastereomers and further isobaric compounds, to separate members of different alkaloid classes within one run and to tolerate significant matrix load. A comparison with methods presented in the literature reveals that a near-generic NACE-MS method for the fast profiling of alkaloids in forensically relevant Plant samples has been developed.
Kristian Bjornstad - One of the best experts on this subject based on the ideXlab platform.
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bioanalytical and clinical evaluation of 103 suspected cases of intoxications with Psychoactive Plant materials
Clinical Toxicology, 2009Co-Authors: Kristian Bjornstad, Peter Hulten, Olof Beck, Anders HelanderAbstract:Introduction. Problems associated with the increasing abuse of Plant-derived Psychoactive substances have recently attracted attention. This study involved bioanalytical and clinical examinations o...
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mass spectrometric investigation of intoxications with Plant derived Psychoactive substances
2009Co-Authors: Kristian BjornstadAbstract:The flora of the world contains many Plants and fungi with stimulant, hallucinogenic and narcotic effects. For centuries, many of these have been used in initiation rites, physical and spiritual healing and rites of divination. Many of the Plants are not placed under any restrictions regarding their use and sale and, with use of the Internet, they are easily obtained. LC-MS/MS and GC-MS methods were developed and used for the detection of 12 Plant-derived Psychoactive substances, in urine samples, in cases of intoxication. The investigated substances were: asarones, atropine, DMT, ephedrine, harmaline, harmine, ibogaine, LSA, mescaline, psilocin, scopolamine and yohimbine. Urine samples (n=462) from patients admitted to the Maria youth clinic were analyzed for the presence of mescaline. No samples were positive for mescaline, but the method was validated using a clinical sample from a German intoxication case. Urine samples (n=103) from patients admitted to emergency departments all over Sweden were investigated for all 12 substances included in this study. All patients either admitted intake of a Psychoactive Plant substance or were suspected thereof. In 41 of the 103 samples at least one of the investigated substances was present. The most common substance was psilocin, found in 22 urine samples. Mydriasis, tachycardia, visual hallucinations, nausea and vomiting were the symptoms most often reported. These symptoms can be regarded as minor or moderate in terms of severity. The results suggest a low occurrence of Psychoactive Plant use in Sweden. Studies were done in attempt to elucidate the metabolic pattern of αand β-asarone in humans. Cis(β)-2,4,5-trimethoxycinnamic acid was regarded to be the most abundant metabolite, evidence of a hydroxylated metabolite, thought to be hydroxylated βasarone, was also found. Today these substances are often marketed on the Internet as “safe” and “legal highs”, which may lead to an increased use and calls for continuous investigation into Psychoactive Plant intoxications. LIST OF PUBLICATIONS I. Bjornstad K, Helander A, Beck O. Development and Clinical Application of an LC-MS-MS Method for Mescaline in Urine. J Anal Toxicol. 2008;32(3):227-31. II. Bjornstad K, Beck O, Helander A. A multi-component LC-MS/MS method for detection of ten Plant-derived Psychoactive substances in urine. J Chromatogr B Analyt Technol Biomed Life Sci, 2009; 877(11-12):1162-1168 III. Bjornstad K, Hulten P, Beck O, Helander A. Bioanalytical and clinical evaluation of 103 suspected cases of intoxications with Psychoactive Plant materials. (Submitted) IV. Bjornstad K, Helander A, Hulten P, Beck O. Bioanalytical Investigation of Asarone in Connection with Acorus calamus Oil Intoxications. (Manuscript) The original articles (I and II) have been printed with permission from the publishers.
Nathalie Martin - One of the best experts on this subject based on the ideXlab platform.
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Nonaqueous capillary electrophoresis-mass spectrometry: a versatile, straightforward tool for the analysis of alkaloids from Psychoactive Plant extracts.
Electrophoresis, 2012Co-Authors: Tjorben Nils Posch, Nathalie Martin, Michael Pütz, Carolin HuhnAbstract:In this study we show that a nonaqueous capillary electrophoresis mass spectrometry (NACE-MS) method carefully optimized by a design of experiment can be applied to a very large number of alkaloids in different Plant extracts. It is possible to characterize the pattern of the Psychoactive alkaloids in several Plant samples and preparations thereof, each presenting different challenges in their analysis. The method is shown to be able to separate structurally closely related substances, diastereomers and further isobaric compounds, to separate members of different alkaloid classes within one run and to tolerate significant matrix load. A comparison with methods presented in the literature reveals that a near-generic NACE-MS method for the fast profiling of alkaloids in forensically relevant Plant samples has been developed.
Michael Pütz - One of the best experts on this subject based on the ideXlab platform.
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Nonaqueous capillary electrophoresis-mass spectrometry: a versatile, straightforward tool for the analysis of alkaloids from Psychoactive Plant extracts.
Electrophoresis, 2012Co-Authors: Tjorben Nils Posch, Nathalie Martin, Michael Pütz, Carolin HuhnAbstract:In this study we show that a nonaqueous capillary electrophoresis mass spectrometry (NACE-MS) method carefully optimized by a design of experiment can be applied to a very large number of alkaloids in different Plant extracts. It is possible to characterize the pattern of the Psychoactive alkaloids in several Plant samples and preparations thereof, each presenting different challenges in their analysis. The method is shown to be able to separate structurally closely related substances, diastereomers and further isobaric compounds, to separate members of different alkaloid classes within one run and to tolerate significant matrix load. A comparison with methods presented in the literature reveals that a near-generic NACE-MS method for the fast profiling of alkaloids in forensically relevant Plant samples has been developed.
