Pubescence

14,000,000 Leading Edge Experts on the ideXlab platform

Scan Science and Technology

Contact Leading Edge Experts & Companies

Scan Science and Technology

Contact Leading Edge Experts & Companies

The Experts below are selected from a list of 279 Experts worldwide ranked by ideXlab platform

Ryoji Takahashi - One of the best experts on this subject based on the ideXlab platform.

  • loss of function mutation of soybean r2r3 myb transcription factor dilutes tawny Pubescence color
    Frontiers in Plant Science, 2020
    Co-Authors: Stephen M. Githiri, Yu Sang, Qingyu Wang, Ryoji Takahashi
    Abstract:

    Pubescence color of soybean is controlled by two genes, T and Td. In the presence of a dominant T allele, dominant and recessive alleles of the Td locus generate tawny and light tawny (or near-gray) Pubescence, respectively. Flavones, responsible for Pubescence color, are synthesized via two copies of flavone synthase II genes (FNS II-1 and FNS II-2). This study was conducted to map and clone the Td gene. Genetic and linkage analysis using an F2 population and F3 families derived from a cross between a Clark near-isogenic line with light tawny Pubescence (genotype: TT tdtd) and a Harosoy near-isogenic line with tawny Pubescence (TT TdTd) revealed a single gene for Pubescence color around the end of chromosome 3. Genome sequence alignment of plant introductions revealed an association between premature stop codons in Glyma.03G258700 (R2R3 MYB transcription factor) and recessive td allele. Cultivars and lines having near-gray or light tawny Pubescence and a gray Pubescence cultivar with td allele had premature stop codons in the gene. These results suggest that Glyma.03G258700 corresponds to the Td gene. It was predominantly expressed in Pubescence. Compared to a tawny Pubescence line, a near-isogenic line with td allele produced extremely small amounts of transcripts of Glyma.03G258700, FNS II-1, and FNS II-2 in Pubescence. The promoter of FNS II-1 and FNS II-2 shared cis-acting regulatory elements for binding of MYB proteins. These results suggest that the wild-type of Glyma.03G258700 protein may bind to the promoter of FNS II genes and upregulates their expression, resulting in increased flavone content and deeper Pubescence color. In contrast, mutated Glyma.03G258700 protein may fail to upregulate the expression of FNS II genes, resulting in decreased flavone content and dilute Pubescence color. These results strongly suggest that soybean Glyma.03G258700 encoding the R2R3 MYB transcription factor corresponds to the Td gene.

  • Loss-of-function mutation of soybean R2R3 MYB transcription factor reduces flavone content and dilutes tawny Pubescence color
    2019
    Co-Authors: Stephen M. Githiri, Yu Sang, Qingyu Wang, Ryoji Takahashi
    Abstract:

    Abstract Background Pubescence color of soybean is controlled by two genes, T and Td. In the presence of a dominant T allele, dominant and recessive alleles of the Td locus generate tawny and light tawny (or near-gray) Pubescence, respectively. Flavones, responsible for Pubescence color, are catalyzed by two copies of flavone synthase II genes (FNS II-1 and FNS II-2). This study was conducted to map and clone the Td gene. Results Genetic and linkage analysis using an F2 population and F3 families derived from a cross between a Clark near-isogenic line with light tawny Pubescence (genotype: TT tdtd) and a Harosoy near-isogenic line with tawny Pubescence (TT TdTd) revealed a single gene for Pubescence color around the end of chromosome 3. Genome sequence alignment of plant introductions revealed an association between premature stop codons in Glyma.03G258700 (R2R3 MYB transcription factor) and recessive td allele. Cultivars and lines having near-gray or light tawny Pubescence and a gray Pubescence cultivar with td allele had premature stop codons in the gene. These results suggest that Glyma.03G258700 corresponds to the Td gene. It was predominantly expressed in Pubescence. Compared to a tawny Pubescence line, a near-isogenic line with td allele produced extremely small amounts of transcripts of Glyma.03G258700, FNS II-1, and FNS II-2 in Pubescence. The promoter of FNS II-1 and FNS II-2 shared cis-acting regulatory elements for binding of MYB proteins. These results suggest that the wild-type of Glyma.03G258700 protein binds to the promoter of FNS II genes and upregulates their expression, resulting in increased flavone content and deeper Pubescence color. In contrast, mutated Glyma.03G258700 protein fails to upregulate the expression of FNS II genes, resulting in decreased flavone content and dilute Pubescence color. Conclusions This study revealed that soybean Glyma.03G258700 encoding the R2R3 MYB transcription factor corresponds to the Td gene. The wild type of MYB protein binds to the promoter of FNS II genes and upregulates their expression, resulting in higher flavone content and deeper Pubescence color. Loss-of-function mutation of the gene fails to promote expression of FNS II genes, resulting in lower flavone content and dilute Pubescence color.

  • analysis of flavonoids in flower petals of soybean near isogenic lines for flower and Pubescence color genes
    Journal of Heredity, 2007
    Co-Authors: Tsukasa Iwashina, Stephen M. Githiri, Eduardo R Benitez, Tomoko Takemura, Junichi Kitajima, Ryoji Takahashi
    Abstract:

    W1, W3, W4, and Wm genes control flower color, whereas T and Td genes control Pubescence color in soybean. W1, W3, Wm, and T are presumed to encode flavonoid 3#5#-hydroxylase (EC 1.14.13.88), dihydroflavonol 4-reductase (EC 1.1.1.219), flavonol synthase (EC 1.14.11.23), and flavonoid 3#-hydroxylase (EC 1.14.13.21), respectively. The objective of this study was to determine the structure of the primary anthocyanin, flavonol, and dihydroflavonol in flower petals. Primary component of anthocyanin in purple flower cultivars Clark (W1W1 w3w3 W4W4 WmWm TT TdTd) and Harosoy (W1W1 w3w3 W4W4 WmWm tt TdTd) was malvidin 3,5-di-O-glucoside with delphinidin 3,5-di-O-glucoside as a minor compound. Primary flavonol and dihydroflavonol were kaempferol 3-O-gentiobioside and aromadendrin 3-O-glucoside, respectively. Quantitative analysis of near-isogenic lines (NILs) for flower or Pubescence color genes, Clark-w1 (white flower), Clark-w4 (near-white flower), Clark-W3w4 (dilute purple flower), Clark-t (gray Pubescence), Clark-td (near-gray Pubescence), Harosoy-wm (magenta flower), and Harosoy-T (tawny Pubescence) was carried out. No anthocyanins were detected in Clark-w1 and Clark-w4, whereas a trace amount was detected in Clark-W3w4. Amount of flavonols and dihydroflavonol in NILs with w1 or w4 were largely similar to the NILs with purple flower suggesting that W1 and W4 affect only anthocyanin biosynthesis. Amount of flavonol glycosides was substantially reduced and dihydroflavonol was increased in Harosoy-wm suggesting that Wm is responsible for the production of flavonol from dihydroflavonol. The recessive wm allele reduces flavonol amount and inhibits co-pigmentation between anthocyanins and flavonols resulting in less bluer (magenta) flower color. Pubescence color genes, T or Td, had no apparent effect on flavonoid biosynthesis in flower petals.

  • analysis of flavonoids in Pubescence of soybean near isogenic lines for Pubescence color loci
    Journal of Heredity, 2006
    Co-Authors: Tsukasa Iwashina, Eduardo R Benitez, Ryoji Takahashi
    Abstract:

    T and Td loci control Pubescence color of soybean with epistatic effects (TT TdTd, tawny; TT tdtd, light tawny or near-gray; tt TdTd or tt tdtd, gray). The objective of this study was to investigate the nature of flavonoids in the Pubescence of nearisogenic lines (NILs) for these loci. Flavonoids were extracted with methanol from Pubescence of cultivar Clark with tawny Pubescence (TT TdTd) and its NILs; from Clark-t with gray Pubescence (tt TdTd) and Clark-td with near-gray Pubescence (TT tdtd); and from a pair of NILs, To7B with tawny (TT TdTd) and To7G with gray Pubescence (tt TdTd). Primary flavonoids were flavone aglycones. Luteolin and apigenin were predominant in NILs with tawny and gray Pubescence, respectively. Small amount of 7-O-glucosides of the 2 flavones were also detected. Alleles at T locus were associated with 3#-hydroxylation in the B-ring of the flavones. The primary flavonoids in Clark-td were luteolin similar to Clark, but its amount was halved. High performance liquid chromatography peaks probably corresponding to isoflavonoids were found only in Clark-tdin 2003. However, the peaks were not observed in 2005. The above results suggest that Td may encode a structural or a regulatory gene controlling flavone biosynthesis. Pigments remained visible in Pubescence after methanol extraction, suggesting that a major part of the pigments was polymerized. Surface rinsing experiments revealed that flavone aglycones exist outside the surface of cells.

  • structure of flavonoid 3 hydroxylase gene for Pubescence color in soybean
    Crop Science, 2005
    Co-Authors: Kyoko Toda, Maiko Akasaka, Emilyn G Dubouzet, Shinji Kawasaki, Ryoji Takahashi
    Abstract:

    The T locus of soybean [Glycine max (L.) Merr.] controls Pubescence and seed coat color and it is presumed to encode a flavonoid 3'-hydroxylase (F3'H). Dominant T and recessive t allele of the locus produce tawny and gray Pubescence, respectively. Alleles at the T locus are associated with chilling tolerance. We previously cloned the entire cDNA of F3'H gene (sf3'h1) from soybean. In this report, the entire F3'H gene was characterized by isolating two genomic clones covering the entire gene. Sequence analysis revealed that F3'H gene consists of three exons and two introns distributed in a 8500-bp DNA segment. The promoter region of the F3'H gene contains a putative G-box, two MYB-binding domains, and TA-repeats. The structure and number of the TA repeats was cultivar-dependent and highly polymorphic. A pair of simple sequence repeat (SSR) primers designated as SoyF3'H was developed to flank the TA-repeats. The SSR band polymorphism cosegregated with genotypes at T locus in 89 F 2 plants segregating for the T locus. The SSR marker may be a useful internal marker of the F3'H gene and is applicable even among cultivars with similar Pubescence color. The promoter sequence information obtained in this report may be useful for investigations on the transcriptional control of F3'H gene as well as transgenic experiments to clarify the relationship between F3'H gene and chilling tolerance in soybean.

Stephen M. Githiri - One of the best experts on this subject based on the ideXlab platform.

  • loss of function mutation of soybean r2r3 myb transcription factor dilutes tawny Pubescence color
    Frontiers in Plant Science, 2020
    Co-Authors: Stephen M. Githiri, Yu Sang, Qingyu Wang, Ryoji Takahashi
    Abstract:

    Pubescence color of soybean is controlled by two genes, T and Td. In the presence of a dominant T allele, dominant and recessive alleles of the Td locus generate tawny and light tawny (or near-gray) Pubescence, respectively. Flavones, responsible for Pubescence color, are synthesized via two copies of flavone synthase II genes (FNS II-1 and FNS II-2). This study was conducted to map and clone the Td gene. Genetic and linkage analysis using an F2 population and F3 families derived from a cross between a Clark near-isogenic line with light tawny Pubescence (genotype: TT tdtd) and a Harosoy near-isogenic line with tawny Pubescence (TT TdTd) revealed a single gene for Pubescence color around the end of chromosome 3. Genome sequence alignment of plant introductions revealed an association between premature stop codons in Glyma.03G258700 (R2R3 MYB transcription factor) and recessive td allele. Cultivars and lines having near-gray or light tawny Pubescence and a gray Pubescence cultivar with td allele had premature stop codons in the gene. These results suggest that Glyma.03G258700 corresponds to the Td gene. It was predominantly expressed in Pubescence. Compared to a tawny Pubescence line, a near-isogenic line with td allele produced extremely small amounts of transcripts of Glyma.03G258700, FNS II-1, and FNS II-2 in Pubescence. The promoter of FNS II-1 and FNS II-2 shared cis-acting regulatory elements for binding of MYB proteins. These results suggest that the wild-type of Glyma.03G258700 protein may bind to the promoter of FNS II genes and upregulates their expression, resulting in increased flavone content and deeper Pubescence color. In contrast, mutated Glyma.03G258700 protein may fail to upregulate the expression of FNS II genes, resulting in decreased flavone content and dilute Pubescence color. These results strongly suggest that soybean Glyma.03G258700 encoding the R2R3 MYB transcription factor corresponds to the Td gene.

  • Loss-of-function mutation of soybean R2R3 MYB transcription factor reduces flavone content and dilutes tawny Pubescence color
    2019
    Co-Authors: Stephen M. Githiri, Yu Sang, Qingyu Wang, Ryoji Takahashi
    Abstract:

    Abstract Background Pubescence color of soybean is controlled by two genes, T and Td. In the presence of a dominant T allele, dominant and recessive alleles of the Td locus generate tawny and light tawny (or near-gray) Pubescence, respectively. Flavones, responsible for Pubescence color, are catalyzed by two copies of flavone synthase II genes (FNS II-1 and FNS II-2). This study was conducted to map and clone the Td gene. Results Genetic and linkage analysis using an F2 population and F3 families derived from a cross between a Clark near-isogenic line with light tawny Pubescence (genotype: TT tdtd) and a Harosoy near-isogenic line with tawny Pubescence (TT TdTd) revealed a single gene for Pubescence color around the end of chromosome 3. Genome sequence alignment of plant introductions revealed an association between premature stop codons in Glyma.03G258700 (R2R3 MYB transcription factor) and recessive td allele. Cultivars and lines having near-gray or light tawny Pubescence and a gray Pubescence cultivar with td allele had premature stop codons in the gene. These results suggest that Glyma.03G258700 corresponds to the Td gene. It was predominantly expressed in Pubescence. Compared to a tawny Pubescence line, a near-isogenic line with td allele produced extremely small amounts of transcripts of Glyma.03G258700, FNS II-1, and FNS II-2 in Pubescence. The promoter of FNS II-1 and FNS II-2 shared cis-acting regulatory elements for binding of MYB proteins. These results suggest that the wild-type of Glyma.03G258700 protein binds to the promoter of FNS II genes and upregulates their expression, resulting in increased flavone content and deeper Pubescence color. In contrast, mutated Glyma.03G258700 protein fails to upregulate the expression of FNS II genes, resulting in decreased flavone content and dilute Pubescence color. Conclusions This study revealed that soybean Glyma.03G258700 encoding the R2R3 MYB transcription factor corresponds to the Td gene. The wild type of MYB protein binds to the promoter of FNS II genes and upregulates their expression, resulting in higher flavone content and deeper Pubescence color. Loss-of-function mutation of the gene fails to promote expression of FNS II genes, resulting in lower flavone content and dilute Pubescence color.

  • analysis of flavonoids in flower petals of soybean near isogenic lines for flower and Pubescence color genes
    Journal of Heredity, 2007
    Co-Authors: Tsukasa Iwashina, Stephen M. Githiri, Eduardo R Benitez, Tomoko Takemura, Junichi Kitajima, Ryoji Takahashi
    Abstract:

    W1, W3, W4, and Wm genes control flower color, whereas T and Td genes control Pubescence color in soybean. W1, W3, Wm, and T are presumed to encode flavonoid 3#5#-hydroxylase (EC 1.14.13.88), dihydroflavonol 4-reductase (EC 1.1.1.219), flavonol synthase (EC 1.14.11.23), and flavonoid 3#-hydroxylase (EC 1.14.13.21), respectively. The objective of this study was to determine the structure of the primary anthocyanin, flavonol, and dihydroflavonol in flower petals. Primary component of anthocyanin in purple flower cultivars Clark (W1W1 w3w3 W4W4 WmWm TT TdTd) and Harosoy (W1W1 w3w3 W4W4 WmWm tt TdTd) was malvidin 3,5-di-O-glucoside with delphinidin 3,5-di-O-glucoside as a minor compound. Primary flavonol and dihydroflavonol were kaempferol 3-O-gentiobioside and aromadendrin 3-O-glucoside, respectively. Quantitative analysis of near-isogenic lines (NILs) for flower or Pubescence color genes, Clark-w1 (white flower), Clark-w4 (near-white flower), Clark-W3w4 (dilute purple flower), Clark-t (gray Pubescence), Clark-td (near-gray Pubescence), Harosoy-wm (magenta flower), and Harosoy-T (tawny Pubescence) was carried out. No anthocyanins were detected in Clark-w1 and Clark-w4, whereas a trace amount was detected in Clark-W3w4. Amount of flavonols and dihydroflavonol in NILs with w1 or w4 were largely similar to the NILs with purple flower suggesting that W1 and W4 affect only anthocyanin biosynthesis. Amount of flavonol glycosides was substantially reduced and dihydroflavonol was increased in Harosoy-wm suggesting that Wm is responsible for the production of flavonol from dihydroflavonol. The recessive wm allele reduces flavonol amount and inhibits co-pigmentation between anthocyanins and flavonols resulting in less bluer (magenta) flower color. Pubescence color genes, T or Td, had no apparent effect on flavonoid biosynthesis in flower petals.

Qingyu Wang - One of the best experts on this subject based on the ideXlab platform.

  • loss of function mutation of soybean r2r3 myb transcription factor dilutes tawny Pubescence color
    Frontiers in Plant Science, 2020
    Co-Authors: Stephen M. Githiri, Yu Sang, Qingyu Wang, Ryoji Takahashi
    Abstract:

    Pubescence color of soybean is controlled by two genes, T and Td. In the presence of a dominant T allele, dominant and recessive alleles of the Td locus generate tawny and light tawny (or near-gray) Pubescence, respectively. Flavones, responsible for Pubescence color, are synthesized via two copies of flavone synthase II genes (FNS II-1 and FNS II-2). This study was conducted to map and clone the Td gene. Genetic and linkage analysis using an F2 population and F3 families derived from a cross between a Clark near-isogenic line with light tawny Pubescence (genotype: TT tdtd) and a Harosoy near-isogenic line with tawny Pubescence (TT TdTd) revealed a single gene for Pubescence color around the end of chromosome 3. Genome sequence alignment of plant introductions revealed an association between premature stop codons in Glyma.03G258700 (R2R3 MYB transcription factor) and recessive td allele. Cultivars and lines having near-gray or light tawny Pubescence and a gray Pubescence cultivar with td allele had premature stop codons in the gene. These results suggest that Glyma.03G258700 corresponds to the Td gene. It was predominantly expressed in Pubescence. Compared to a tawny Pubescence line, a near-isogenic line with td allele produced extremely small amounts of transcripts of Glyma.03G258700, FNS II-1, and FNS II-2 in Pubescence. The promoter of FNS II-1 and FNS II-2 shared cis-acting regulatory elements for binding of MYB proteins. These results suggest that the wild-type of Glyma.03G258700 protein may bind to the promoter of FNS II genes and upregulates their expression, resulting in increased flavone content and deeper Pubescence color. In contrast, mutated Glyma.03G258700 protein may fail to upregulate the expression of FNS II genes, resulting in decreased flavone content and dilute Pubescence color. These results strongly suggest that soybean Glyma.03G258700 encoding the R2R3 MYB transcription factor corresponds to the Td gene.

  • Loss-of-function mutation of soybean R2R3 MYB transcription factor reduces flavone content and dilutes tawny Pubescence color
    2019
    Co-Authors: Stephen M. Githiri, Yu Sang, Qingyu Wang, Ryoji Takahashi
    Abstract:

    Abstract Background Pubescence color of soybean is controlled by two genes, T and Td. In the presence of a dominant T allele, dominant and recessive alleles of the Td locus generate tawny and light tawny (or near-gray) Pubescence, respectively. Flavones, responsible for Pubescence color, are catalyzed by two copies of flavone synthase II genes (FNS II-1 and FNS II-2). This study was conducted to map and clone the Td gene. Results Genetic and linkage analysis using an F2 population and F3 families derived from a cross between a Clark near-isogenic line with light tawny Pubescence (genotype: TT tdtd) and a Harosoy near-isogenic line with tawny Pubescence (TT TdTd) revealed a single gene for Pubescence color around the end of chromosome 3. Genome sequence alignment of plant introductions revealed an association between premature stop codons in Glyma.03G258700 (R2R3 MYB transcription factor) and recessive td allele. Cultivars and lines having near-gray or light tawny Pubescence and a gray Pubescence cultivar with td allele had premature stop codons in the gene. These results suggest that Glyma.03G258700 corresponds to the Td gene. It was predominantly expressed in Pubescence. Compared to a tawny Pubescence line, a near-isogenic line with td allele produced extremely small amounts of transcripts of Glyma.03G258700, FNS II-1, and FNS II-2 in Pubescence. The promoter of FNS II-1 and FNS II-2 shared cis-acting regulatory elements for binding of MYB proteins. These results suggest that the wild-type of Glyma.03G258700 protein binds to the promoter of FNS II genes and upregulates their expression, resulting in increased flavone content and deeper Pubescence color. In contrast, mutated Glyma.03G258700 protein fails to upregulate the expression of FNS II genes, resulting in decreased flavone content and dilute Pubescence color. Conclusions This study revealed that soybean Glyma.03G258700 encoding the R2R3 MYB transcription factor corresponds to the Td gene. The wild type of MYB protein binds to the promoter of FNS II genes and upregulates their expression, resulting in higher flavone content and deeper Pubescence color. Loss-of-function mutation of the gene fails to promote expression of FNS II genes, resulting in lower flavone content and dilute Pubescence color.

Yu Sang - One of the best experts on this subject based on the ideXlab platform.

  • loss of function mutation of soybean r2r3 myb transcription factor dilutes tawny Pubescence color
    Frontiers in Plant Science, 2020
    Co-Authors: Stephen M. Githiri, Yu Sang, Qingyu Wang, Ryoji Takahashi
    Abstract:

    Pubescence color of soybean is controlled by two genes, T and Td. In the presence of a dominant T allele, dominant and recessive alleles of the Td locus generate tawny and light tawny (or near-gray) Pubescence, respectively. Flavones, responsible for Pubescence color, are synthesized via two copies of flavone synthase II genes (FNS II-1 and FNS II-2). This study was conducted to map and clone the Td gene. Genetic and linkage analysis using an F2 population and F3 families derived from a cross between a Clark near-isogenic line with light tawny Pubescence (genotype: TT tdtd) and a Harosoy near-isogenic line with tawny Pubescence (TT TdTd) revealed a single gene for Pubescence color around the end of chromosome 3. Genome sequence alignment of plant introductions revealed an association between premature stop codons in Glyma.03G258700 (R2R3 MYB transcription factor) and recessive td allele. Cultivars and lines having near-gray or light tawny Pubescence and a gray Pubescence cultivar with td allele had premature stop codons in the gene. These results suggest that Glyma.03G258700 corresponds to the Td gene. It was predominantly expressed in Pubescence. Compared to a tawny Pubescence line, a near-isogenic line with td allele produced extremely small amounts of transcripts of Glyma.03G258700, FNS II-1, and FNS II-2 in Pubescence. The promoter of FNS II-1 and FNS II-2 shared cis-acting regulatory elements for binding of MYB proteins. These results suggest that the wild-type of Glyma.03G258700 protein may bind to the promoter of FNS II genes and upregulates their expression, resulting in increased flavone content and deeper Pubescence color. In contrast, mutated Glyma.03G258700 protein may fail to upregulate the expression of FNS II genes, resulting in decreased flavone content and dilute Pubescence color. These results strongly suggest that soybean Glyma.03G258700 encoding the R2R3 MYB transcription factor corresponds to the Td gene.

  • Loss-of-function mutation of soybean R2R3 MYB transcription factor reduces flavone content and dilutes tawny Pubescence color
    2019
    Co-Authors: Stephen M. Githiri, Yu Sang, Qingyu Wang, Ryoji Takahashi
    Abstract:

    Abstract Background Pubescence color of soybean is controlled by two genes, T and Td. In the presence of a dominant T allele, dominant and recessive alleles of the Td locus generate tawny and light tawny (or near-gray) Pubescence, respectively. Flavones, responsible for Pubescence color, are catalyzed by two copies of flavone synthase II genes (FNS II-1 and FNS II-2). This study was conducted to map and clone the Td gene. Results Genetic and linkage analysis using an F2 population and F3 families derived from a cross between a Clark near-isogenic line with light tawny Pubescence (genotype: TT tdtd) and a Harosoy near-isogenic line with tawny Pubescence (TT TdTd) revealed a single gene for Pubescence color around the end of chromosome 3. Genome sequence alignment of plant introductions revealed an association between premature stop codons in Glyma.03G258700 (R2R3 MYB transcription factor) and recessive td allele. Cultivars and lines having near-gray or light tawny Pubescence and a gray Pubescence cultivar with td allele had premature stop codons in the gene. These results suggest that Glyma.03G258700 corresponds to the Td gene. It was predominantly expressed in Pubescence. Compared to a tawny Pubescence line, a near-isogenic line with td allele produced extremely small amounts of transcripts of Glyma.03G258700, FNS II-1, and FNS II-2 in Pubescence. The promoter of FNS II-1 and FNS II-2 shared cis-acting regulatory elements for binding of MYB proteins. These results suggest that the wild-type of Glyma.03G258700 protein binds to the promoter of FNS II genes and upregulates their expression, resulting in increased flavone content and deeper Pubescence color. In contrast, mutated Glyma.03G258700 protein fails to upregulate the expression of FNS II genes, resulting in decreased flavone content and dilute Pubescence color. Conclusions This study revealed that soybean Glyma.03G258700 encoding the R2R3 MYB transcription factor corresponds to the Td gene. The wild type of MYB protein binds to the promoter of FNS II genes and upregulates their expression, resulting in higher flavone content and deeper Pubescence color. Loss-of-function mutation of the gene fails to promote expression of FNS II genes, resulting in lower flavone content and dilute Pubescence color.

Kunihiko Komatsu - One of the best experts on this subject based on the ideXlab platform.

  • genetic analysis of antixenosis resistance to the common cutworm spodoptera litura fabricius and its relationship with Pubescence characteristics in soybean glycine max l merr
    Breeding Science, 2012
    Co-Authors: Kunihiko Komatsu, Takashi Sayama, Masao Ishimoto, Masakazu Takahashi, Motoki Takahashi
    Abstract:

    The common cutworm (CCW, Spodoptera litura Fabricius) is one of the most serious pests of soybean (Glycine max (L.) Merr.). Previously, two quantitative trait loci (QTLs) for antibiosis resistance to CCW, CCW-1 and CCW-2, were detected in the resistant cultivar Himeshirazu. In this study, we conducted an anti-xenosis bioassay using a recombinant inbred population derived from a cross between a susceptible cultivar Fukuyutaka and Himeshirazu to perform QTL analysis. Two QTLs for antixenosis resistance, qRslx1 and qRslx2, were identified on Chrs 7 and 12, and the resistant alleles of qRslx1 and qRslx2 were derived from Himeshirazu and Fukuyutaka, respectively. The position of qRslx1 is similar to that of CCW-1. We also analyzed Pubescence characteristics because they have been reported to be associated with soybean insect resistance. Two QTLs for Pubescence length (on Chrs 7 and 12) and two QTLs for Pubescence density (on Chrs 1 and 12) were identified. The Pubescence QTLs on Chrs 7 and 12 were located near qRslx1 and qRslx2, respectively. These results suggest that the antixenosis resistance could be controlled genetically by the identified QTLs and that the Pubescence characteristics might contribute to the soybean antixenosis resistance to CCW.

  • quantitative trait loci mapping of Pubescence density and flowering time of insect resistant soybean glycine max l merr
    Genetics and Molecular Biology, 2007
    Co-Authors: Kunihiko Komatsu, Masakazu Takahashi, Shiori Okuda, Ryoichi Matsunaga, Yoshinori Nakazawa
    Abstract:

    Analysis of antibiosis resistance to common cutworm (Spodoptera litura Fabricius) in soybean (Glycine max (L.) Merr.) has progressed significantly, but the immediate cause remains unknown. We performed quantitative trait loci (QTL) analysis of Pubescence density and plant development stage because these factors are assumed to be the immediate cause of resistance to cutworm. The QTLs for Pubescence appeared to be identical to the previously detected the Pd1 and Ps loci controlling Pubescence density. We found no candidate loci for flowering time QTLs, although one could be identical to the gene governing the long-juvenile trait or to the E6 loci controlling maturity. None of the QTLs overlapped with the QTLs for antibiosis resistance.