The Experts below are selected from a list of 264 Experts worldwide ranked by ideXlab platform
Xianming Chen - One of the best experts on this subject based on the ideXlab platform.
-
Differential resistance to stripe rust (Puccinia striiformis) in collections of basin wild rye (Leymus cinereus)
Plant Health Progress, 2020Co-Authors: Frank M. Dugan, Meinan Wang, Michael J. Cashman, Richard C. Johnson, Xianming ChenAbstract:Dugan, F. M., Cashman, M. J., Johnson, R. C., Wang, M. N., and Chen, X. M. 201X. Differential resistance to stripe rust (Puccinia striiformis) in collections of Basin wild rye (Leymus cinereus). Plant Health Progress doi:10.1094/PHP-RS-14-0002 Differential resistance to stripe rust (Puccinia striiformis) in a planting of 111 wild collections of Basin wild rye (Leymus cinereus) was noted 2011-2013. In 2011, rust severity was rated on a scale of 1-9. Much lighter infection in 2012 and 2013 was rated as the number of symptomatic leaves per plant divided by plant circumference (to adjust for plant size). Effect of collection was significant in 2011 (P = 0.0042), 2012 (P = 0.0032), and 2013 (P = 0.0095), with a relatively weak (0.23) but significant (P = 0.0149) correlation between 2011 and 2012 and a stronger (0.38) and highly significant (P < 0.0001) correlation between 2012 and 2013. Correlation between results of 2011 and 2013 was near zero and insignificant unless the ratings from 2011 were adjusted for plant size. Representative resistant and susceptible collections will be conserved as accessions in the National Plant Germplasm System.
-
Wheat-Puccinia striiformis Interactions
Stripe Rust, 2020Co-Authors: Zhensheng Kang, Jie Zhao, Xiaojie Wang, Chunlei Tang, Yulin Cheng, Xianming ChenAbstract:Owing to the scientific and economic importance, the antagonistic wheat-Puccinia striiformis f. sp. tritici (Pst) pathosystem has been a focus of research over the past five decades. With the recent discovery of barberry as alternate hosts, Pst has been confirmed to be heteroecious, and the roles of the alternate hosts in disease epidemiology and pathogen variation have been studied. Meanwhile, considerable effort has been taken and significant progress has been made to unravel the wheat-Pst interactions. Histological and cytological studies have provided basic information on infection strategies used by the pathogen and defense responses from the host during wheat-Pst interactions and identified cellular components involved in the interactions. Physiological studies demonstrated the essential changes in Pst infected wheat leaves, unraveling the damage of the pathogen and the countermeasures of the host plants. Transcriptome and genome sequencing has revealed the molecular features and dynamics of the wheat-Pst pathosystem. Extensive molecular analyses have led to the identification of major components in the wheat resistance responses. Studies of wheat-Pst interactions have now entered a new phase in which cellular and molecular approaches are being used. In this chapter, we present the achievements made in the histological and cytological study of wheat-Pst interactions as well as physiological plant pathology. Furthermore, the new insights into wheat immunity provided by the sequence resources and advanced genomic technologies were discussed. Overall, this chapter focuses on the cellular biology of the stripe rust fungus and the wheat-Pst interactions, and integrates the emerging data from molecular analyses with the histocytological observations.
-
Genome Sequence Resource of a Puccinia striiformis Isolate Infecting Wheatgrass.
Phytopathology, 2019Co-Authors: Yuxiang Li, Meinan Wang, Xianming ChenAbstract:: Stripe rust caused by Puccinia striiformis is a disastrous disease of cereal crops and various grasses. To date, 14 stripe rust genomes are publicly available, including 13 P. striiformis f. sp. tritici and 1 P. striiformis f. sp. hordei. In this study, one isolate (11-281) of P. striiformis collected from wheatgrass (Agropyron cristatum), which is avirulent to most of standard differential genotypes of wheat and barley, was sequenced, assembled, and annotated. The sequences were assembled to a draft genome of 84.75 Mb, which is comparable with previously sequenced P. striiformis f. sp. tritici and P. striiformis f. sp. hordei isolates. The draft genome comprised 381 scaffolds and contained 1,829 predicted secreted proteins. The high-quality draft genome of the isolate is a valuable resource in shedding light on the evolution and pathogenicity of P. striiformis.
-
Differential sensitivity among Puccinia striiformis f. sp. tritici isolates to propiconazole and pyraclostrobin fungicides
Canadian Journal of Plant Pathology-revue Canadienne De Phytopathologie, 2019Co-Authors: Zhanhai Kang, Xing Li, Meinan Wang, Xianming ChenAbstract:AbstractStripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a destructive disease of wheat in the USA and fungicides have been used to manage the disease. However, it is not clear ...
-
Genome Sequence Resources for the Wheat Stripe Rust Pathogen (Puccinia striiformis f. sp. tritici) and the Barley Stripe Rust Pathogen (Puccinia striiformis f. sp. hordei).
Molecular Plant-microbe Interactions, 2018Co-Authors: Meinan Wang, Omar E. Cornejo, Scot H. Hulbert, Xianming ChenAbstract:: Puccinia striiformis f. sp. tritici causes devastating stripe (yellow) rust on wheat and P. striiformis f. sp. hordei causes stripe rust on barley. Several P. striiformis f. sp. tritici genomes are available, but no P. striiformis f. sp. hordei genome is available. More genomes of P. striiformis f. sp. tritici and P. striiformis f. sp. hordei are needed to understand the genome evolution and molecular mechanisms of their pathogenicity. We sequenced P. striiformis f. sp. tritici isolate 93-210 and P. striiformis f. sp. hordei isolate 93TX-2, using PacBio and Illumina technologies and RNA sequencing. Their genomic sequences were assembled to contigs with high continuity and showed significant structural differences. The circular mitochondria genomes of both were complete. These genomes provide high-quality resources for deciphering the genomic basis of rapid evolution and host adaptation, identifying genes for avirulence and other important traits, and studying host-pathogen interactions.
Zhensheng Kang - One of the best experts on this subject based on the ideXlab platform.
-
RAPD markers of Puccinia striiformis f.sp.tritici in China
Journal of Northwest Sci-Tech University of Agriculture and Forestry, 2020Co-Authors: Zhensheng Kang, Jie Zhao, Lili HuangAbstract:Rapid identification of the races of Puccinia striiformis f．sp．tritici by molecular technique was very important to monitor and control wheat stripe rust in China．In this paper, five physiological races of Puccinia striiformis f．sp．tritici were analysed with 210 primers by RAPD technique．The special RAPD fragments of CY31, CY29, CY23 and Shuiyuan pathotype were founded successfully．The results here indicates that a molecular identification system for the races of Puccinia striiformis f．sp．tritici in China could be established through cosmically searching special RAPD fragments of different races．
-
The development of SCAR detection marker of {\sl Puccinia striiformis} f.sp. {\sl tritici} race CY31 in China
2020Co-Authors: Zhensheng Kang, Lili Huang, Wenming Zheng, Zhenqi LiAbstract:Rapid identification of the races of Puccinia striiformis f.sp. tritici by molecular technique was very important to monitor and control of wheat stripe rust in China. In this paper, a molecular identification method for a Chinese race of Puccinia striiformis f.sp. tritici CY31 was developed by means of SCAR-PCR technique. Five Chinese races of P. striiformis f.sp. tritici were analysed with 210 primers by RAPD technique. A special DNA fragment of race CY31 was founded and cloned. Based on the sequencing result, specific PCR primers were designed and a SCAR marker for race CY31 was obtained successfully. The results here indicates that the identification of the races of Puccinia striiformis f.sp. tritici could be improved greatly through cosmically searching special RAPD fragments of different races.
-
Wheat-Puccinia striiformis Interactions
Stripe Rust, 2020Co-Authors: Zhensheng Kang, Jie Zhao, Xiaojie Wang, Chunlei Tang, Yulin Cheng, Xianming ChenAbstract:Owing to the scientific and economic importance, the antagonistic wheat-Puccinia striiformis f. sp. tritici (Pst) pathosystem has been a focus of research over the past five decades. With the recent discovery of barberry as alternate hosts, Pst has been confirmed to be heteroecious, and the roles of the alternate hosts in disease epidemiology and pathogen variation have been studied. Meanwhile, considerable effort has been taken and significant progress has been made to unravel the wheat-Pst interactions. Histological and cytological studies have provided basic information on infection strategies used by the pathogen and defense responses from the host during wheat-Pst interactions and identified cellular components involved in the interactions. Physiological studies demonstrated the essential changes in Pst infected wheat leaves, unraveling the damage of the pathogen and the countermeasures of the host plants. Transcriptome and genome sequencing has revealed the molecular features and dynamics of the wheat-Pst pathosystem. Extensive molecular analyses have led to the identification of major components in the wheat resistance responses. Studies of wheat-Pst interactions have now entered a new phase in which cellular and molecular approaches are being used. In this chapter, we present the achievements made in the histological and cytological study of wheat-Pst interactions as well as physiological plant pathology. Furthermore, the new insights into wheat immunity provided by the sequence resources and advanced genomic technologies were discussed. Overall, this chapter focuses on the cellular biology of the stripe rust fungus and the wheat-Pst interactions, and integrates the emerging data from molecular analyses with the histocytological observations.
-
Alternate Hosts of Puccinia striiformis f. sp. tritici and Their Role.
Pathogens (Basel Switzerland), 2020Co-Authors: Sajid Mehmood, Lili Huang, Jie Zhao, Marina Sajid, Zhensheng KangAbstract:Understanding the interactions between the host and the pathogen is important in developing resistant cultivars and strategies for controlling the disease. Since the discovery of Berberis and Mahonia spp. as alternate hosts of the wheat stripe rust pathogen, Puccinia striiformis Westend. f. sp. tritici Erikss. (Pst), their possible role in generating new races of Pst through sexual reproduction has become a hot topic. To date, all the investigations about the role of alternate hosts in the occurrence of the wheat stripe rust epidemics revealed that it depends on alternate host species and environmental conditions. In this review, we summarized the current status of alternate hosts of Pst, their interactions with the pathogen, their importance in genetic diversity and disease epidemics. Most importantly, the recent research progress in understanding the role of alternate hosts of Pst is provided.
-
A novel narnavirus isolated from the wheat stripe rust fungus Puccinia striiformis f. sp. tritici.
Archives of Virology, 2020Co-Authors: Yanhui Zhang, Xiaofei Liang, Li Zheng, Jing Zhao, Zhensheng KangAbstract:The complete genome of a novel fungal virus, Puccinia striiformis narnavirus 1 (PsNV1), was sequenced and analyzed. The full-length cDNA sequence is 2340 bp in length with a GC content of 50.04%. PsNV1 contains a single open reading frame (ORF), which encodes a putative RNA-dependent RNA polymerase (RdRp) of 741 amino acids with a molecular mass of 81.8 kDa. RdRp phylogeny showed that PsNV1 grouped together with Fusarium poae narnavirus 1 (FpNV1) as a sister branch of narnaviruses, forming a distinct clade. The results of genome sequence comparisons and phylogenetic analysis indicate that PsNV1 is a new member in the genus Narnavirus. To our knowledge, this is the first report of a narnavirus genome sequence in the obligately parasitic fungus Puccinia striiformis f. sp. tritici.
Scot H. Hulbert - One of the best experts on this subject based on the ideXlab platform.
-
Genome Sequence Resources for the Wheat Stripe Rust Pathogen (Puccinia striiformis f. sp. tritici) and the Barley Stripe Rust Pathogen (Puccinia striiformis f. sp. hordei).
Molecular Plant-microbe Interactions, 2018Co-Authors: Meinan Wang, Omar E. Cornejo, Scot H. Hulbert, Xianming ChenAbstract:: Puccinia striiformis f. sp. tritici causes devastating stripe (yellow) rust on wheat and P. striiformis f. sp. hordei causes stripe rust on barley. Several P. striiformis f. sp. tritici genomes are available, but no P. striiformis f. sp. hordei genome is available. More genomes of P. striiformis f. sp. tritici and P. striiformis f. sp. hordei are needed to understand the genome evolution and molecular mechanisms of their pathogenicity. We sequenced P. striiformis f. sp. tritici isolate 93-210 and P. striiformis f. sp. hordei isolate 93TX-2, using PacBio and Illumina technologies and RNA sequencing. Their genomic sequences were assembled to contigs with high continuity and showed significant structural differences. The circular mitochondria genomes of both were complete. These genomes provide high-quality resources for deciphering the genomic basis of rapid evolution and host adaptation, identifying genes for avirulence and other important traits, and studying host-pathogen interactions.
-
generation and analysis of expression sequence tags from haustoria of the wheat stripe rust fungus Puccinia striiformis f sp tritici
BMC Genomics, 2009Co-Authors: Zhensheng Kang, Xiaojie Wang, Xianming Chen, Scot H. HulbertAbstract:Background Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat (Triticum aestivum L.) worldwide. In spite of its agricultural importance, the genomics and genetics of the pathogen are poorly characterized. Pst transcripts from urediniospores and germinated urediniospores have been examined previously, but little is known about genes expressed during host infection. Some genes involved in virulence in other rust fungi have been found to be specifically expressed in haustoria. Therefore, the objective of this study was to generate a cDNA library to characterize genes expressed in haustoria of Pst.
Xiaojie Wang - One of the best experts on this subject based on the ideXlab platform.
-
Wheat-Puccinia striiformis Interactions
Stripe Rust, 2020Co-Authors: Zhensheng Kang, Jie Zhao, Xiaojie Wang, Chunlei Tang, Yulin Cheng, Xianming ChenAbstract:Owing to the scientific and economic importance, the antagonistic wheat-Puccinia striiformis f. sp. tritici (Pst) pathosystem has been a focus of research over the past five decades. With the recent discovery of barberry as alternate hosts, Pst has been confirmed to be heteroecious, and the roles of the alternate hosts in disease epidemiology and pathogen variation have been studied. Meanwhile, considerable effort has been taken and significant progress has been made to unravel the wheat-Pst interactions. Histological and cytological studies have provided basic information on infection strategies used by the pathogen and defense responses from the host during wheat-Pst interactions and identified cellular components involved in the interactions. Physiological studies demonstrated the essential changes in Pst infected wheat leaves, unraveling the damage of the pathogen and the countermeasures of the host plants. Transcriptome and genome sequencing has revealed the molecular features and dynamics of the wheat-Pst pathosystem. Extensive molecular analyses have led to the identification of major components in the wheat resistance responses. Studies of wheat-Pst interactions have now entered a new phase in which cellular and molecular approaches are being used. In this chapter, we present the achievements made in the histological and cytological study of wheat-Pst interactions as well as physiological plant pathology. Furthermore, the new insights into wheat immunity provided by the sequence resources and advanced genomic technologies were discussed. Overall, this chapter focuses on the cellular biology of the stripe rust fungus and the wheat-Pst interactions, and integrates the emerging data from molecular analyses with the histocytological observations.
-
TaMCA1, a regulator of cell death, is important for the interaction between wheat and Puccinia striiformis.
Scientific Reports, 2016Co-Authors: Xiaojie Wang, Kang Wang, Huayi Li, Xiaoyuan Duan, Chunlei Tang, Zhensheng KangAbstract:TaMCA1 , a regulator of cell death, is important for the interaction between wheat and Puccinia striiformis
-
wheat defense genes in fungal Puccinia striiformis infection
Functional & Integrative Genomics, 2010Co-Authors: Jie Zhao, Lili Huang, Xiaojie Wang, Xianming Chen, Zhipeng Qu, Xiumei Yu, Chenfang Wang, Xiudao Yu, Zhensheng KangAbstract:Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most important diseases of wheat worldwide. To isolate defense-related genes against the pathogen, a suppression subtractive hybridization library was constructed for an incompatible interaction. From the library, 652 sequences were determined to be unigenes, of which 31 were determined as genes involved in signal transduction and 77 were predicted to encode defense-related proteins. Expression patterns of 12 selected signal transduction and defense-related genes were determined using quantitative real-time polymerase chain reaction. Signal transduction genes started increasing their expression at 12 h post inoculation (hpi), and expressions of the most of the transport and resistance-related genes were induced at 18 hpi. The gene expression results indicate specific molecular and cellular activities during the incompatible interaction between wheat and the stripe rust pathogen. In general, the expression increase of wheat signal transduction genes soon after inoculation with the pathogen inducing various defense-related genes, including reactive oxygen species, ATP-binding cassette (ABC) transporters, pathogenesis-related proteins, and genes involved in the phenylpropanoid pathway. The activities of these defense genes work in a sequential and concerted manner to result in a hypersensitive response.
-
generation and analysis of expression sequence tags from haustoria of the wheat stripe rust fungus Puccinia striiformis f sp tritici
BMC Genomics, 2009Co-Authors: Zhensheng Kang, Xiaojie Wang, Xianming Chen, Scot H. HulbertAbstract:Background Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat (Triticum aestivum L.) worldwide. In spite of its agricultural importance, the genomics and genetics of the pathogen are poorly characterized. Pst transcripts from urediniospores and germinated urediniospores have been examined previously, but little is known about genes expressed during host infection. Some genes involved in virulence in other rust fungi have been found to be specifically expressed in haustoria. Therefore, the objective of this study was to generate a cDNA library to characterize genes expressed in haustoria of Pst.
-
cdna aflp analysis reveals differential gene expression in compatible interaction of wheat challenged with Puccinia striiformis f sp tritici
BMC Genomics, 2009Co-Authors: Xiaojie Wang, Jie Zhao, Lili Huang, Chunlei Tang, Xianming Chen, Zhipeng Qu, Chenfang Wang, Gang Zhang, Yingchun Li, Zhensheng KangAbstract:Puccinia striiformis f. sp. tritici is a fungal pathogen causing stripe rust, one of the most important wheat diseases worldwide. The fungus is strictly biotrophic and thus, completely dependent on living host cells for its reproduction, which makes it difficult to study genes of the pathogen. In spite of its economic importance, little is known about the molecular basis of compatible interaction between the pathogen and wheat host. In this study, we identified wheat and P. striiformis genes associated with the infection process by conducting a large-scale transcriptomic analysis using cDNA-AFLP.
Lili Huang - One of the best experts on this subject based on the ideXlab platform.
-
RAPD markers of Puccinia striiformis f.sp.tritici in China
Journal of Northwest Sci-Tech University of Agriculture and Forestry, 2020Co-Authors: Zhensheng Kang, Jie Zhao, Lili HuangAbstract:Rapid identification of the races of Puccinia striiformis f．sp．tritici by molecular technique was very important to monitor and control wheat stripe rust in China．In this paper, five physiological races of Puccinia striiformis f．sp．tritici were analysed with 210 primers by RAPD technique．The special RAPD fragments of CY31, CY29, CY23 and Shuiyuan pathotype were founded successfully．The results here indicates that a molecular identification system for the races of Puccinia striiformis f．sp．tritici in China could be established through cosmically searching special RAPD fragments of different races．
-
The development of SCAR detection marker of {\sl Puccinia striiformis} f.sp. {\sl tritici} race CY31 in China
2020Co-Authors: Zhensheng Kang, Lili Huang, Wenming Zheng, Zhenqi LiAbstract:Rapid identification of the races of Puccinia striiformis f.sp. tritici by molecular technique was very important to monitor and control of wheat stripe rust in China. In this paper, a molecular identification method for a Chinese race of Puccinia striiformis f.sp. tritici CY31 was developed by means of SCAR-PCR technique. Five Chinese races of P. striiformis f.sp. tritici were analysed with 210 primers by RAPD technique. A special DNA fragment of race CY31 was founded and cloned. Based on the sequencing result, specific PCR primers were designed and a SCAR marker for race CY31 was obtained successfully. The results here indicates that the identification of the races of Puccinia striiformis f.sp. tritici could be improved greatly through cosmically searching special RAPD fragments of different races.
-
Alternate Hosts of Puccinia striiformis f. sp. tritici and Their Role.
Pathogens (Basel Switzerland), 2020Co-Authors: Sajid Mehmood, Lili Huang, Jie Zhao, Marina Sajid, Zhensheng KangAbstract:Understanding the interactions between the host and the pathogen is important in developing resistant cultivars and strategies for controlling the disease. Since the discovery of Berberis and Mahonia spp. as alternate hosts of the wheat stripe rust pathogen, Puccinia striiformis Westend. f. sp. tritici Erikss. (Pst), their possible role in generating new races of Pst through sexual reproduction has become a hot topic. To date, all the investigations about the role of alternate hosts in the occurrence of the wheat stripe rust epidemics revealed that it depends on alternate host species and environmental conditions. In this review, we summarized the current status of alternate hosts of Pst, their interactions with the pathogen, their importance in genetic diversity and disease epidemics. Most importantly, the recent research progress in understanding the role of alternate hosts of Pst is provided.
-
a novel fungal hyperparasite of Puccinia striiformis f sp tritici the causal agent of wheat stripe rust
PLOS ONE, 2014Co-Authors: Gangming Zhan, Lili Huang, Xianming Chen, Yuan Tian, Fuping Wang, Min Jiao, Zhensheng KangAbstract:Puccinia striiformis f. sp. tritici (Pst), the causal fungus of wheat stripe rust, was previously reported to be infected by Lecanicillium lecanii, Microdochium nivale and Typhula idahoensis. Here, we report a novel hyperparasite on Pst. This hyperparasitic fungus was identified as Cladosporium cladosporioides (Fresen.) GA de Vries based on morphological characteristics observed by light and scanning electron microscopy together with molecular data. The hyperparasite reduced the production and viability of urediniospores and, therefore, could potentially be used for biological control of wheat stripe rust.
-
wheat defense genes in fungal Puccinia striiformis infection
Functional & Integrative Genomics, 2010Co-Authors: Jie Zhao, Lili Huang, Xiaojie Wang, Xianming Chen, Zhipeng Qu, Xiumei Yu, Chenfang Wang, Xiudao Yu, Zhensheng KangAbstract:Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most important diseases of wheat worldwide. To isolate defense-related genes against the pathogen, a suppression subtractive hybridization library was constructed for an incompatible interaction. From the library, 652 sequences were determined to be unigenes, of which 31 were determined as genes involved in signal transduction and 77 were predicted to encode defense-related proteins. Expression patterns of 12 selected signal transduction and defense-related genes were determined using quantitative real-time polymerase chain reaction. Signal transduction genes started increasing their expression at 12 h post inoculation (hpi), and expressions of the most of the transport and resistance-related genes were induced at 18 hpi. The gene expression results indicate specific molecular and cellular activities during the incompatible interaction between wheat and the stripe rust pathogen. In general, the expression increase of wheat signal transduction genes soon after inoculation with the pathogen inducing various defense-related genes, including reactive oxygen species, ATP-binding cassette (ABC) transporters, pathogenesis-related proteins, and genes involved in the phenylpropanoid pathway. The activities of these defense genes work in a sequential and concerted manner to result in a hypersensitive response.
