The Experts below are selected from a list of 84 Experts worldwide ranked by ideXlab platform
Qun Fang - One of the best experts on this subject based on the ideXlab platform.
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sequential operation droplet array an automated microfluidic platform for picoliter scale liquid handling analysis and screening
Analytical Chemistry, 2013Co-Authors: Yunxia Zhang, Qun FangAbstract:This contribution describes a sequential operation droplet array (SODA) system, a fully automated droplet-based microfluidic system capable of performing picoliter-scale liquid manipulation, analysis, and screening. The SODA system was built using a tapered capillary-syringe Pump Module and a two-dimensional (2D) oil-covered droplet array installed on an x–y–z translation stage. With the system, we developed a novel picoliter-scale droplet depositing technique for forming a 2D picoliter-droplet array. On this basis, an automated droplet manipulation method with picoliter precision was established using the programmable combination of the capillary-based liquid aspirating–depositing and the moving of the oil-covered droplet array, the so-called “aspirating–depositing–moving” (ADM) method. Differing from the previously reported droplet systems based on microchips, microcapillaries, or digital microfluidics, this method can achieve complete and flexible droplet manipulations, including droplet assembling, ge...
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sequential operation droplet array an automated microfluidic platform for picoliter scale liquid handling analysis and screening
Analytical Chemistry, 2013Co-Authors: Ying Zhu, Yunxia Zhang, Longfei Cai, Qun FangAbstract:This contribution describes a sequential operation droplet array (SODA) system, a fully automated droplet-based microfluidic system capable of performing picoliter-scale liquid manipulation, analysis, and screening. The SODA system was built using a tapered capillary-syringe Pump Module and a two-dimensional (2D) oil-covered droplet array installed on an x-y-z translation stage. With the system, we developed a novel picoliter-scale droplet depositing technique for forming a 2D picoliter-droplet array. On this basis, an automated droplet manipulation method with picoliter precision was established using the programmable combination of the capillary-based liquid aspirating-depositing and the moving of the oil-covered droplet array, the so-called "aspirating-depositing-moving" (ADM) method. Differing from the previously reported droplet systems based on microchips, microcapillaries, or digital microfluidics, this method can achieve complete and flexible droplet manipulations, including droplet assembling, generation, indexing, transferring, splitting, and fusion in the picoliter range, endowing the present system with ultralow sample/reagent consumptions and substantial versatility in analysis and screening for multiple different samples. To demonstrate its feasibility and versatility, we applied the SODA system in multiple experiments required in drug screening, including the screening of inhibitors for capases-1 from a chemical library, the measurement of IC50 values for the identified inhibitors, and the screening of the synergistic effect of multiple inhibitors. In the experiments, the consumptions of samples and reagents are only 60-180 pL for each droplet microreactor, which are commonly 3-5 orders of magnitude lower than those of conventional multiwell plate systems, and 1-2 orders of magnitude lower than other droplet-based microfluidic systems for multiple sample screening. The ability of the SODA system in performing complicated and multistep droplet manipulations was further demonstrated in the serial dilution of nanoliter-scale inhibitor droplets with concentrations spanning 6 orders of magnitude for IC50 profiling, which includes droplet generation, indexing, splitting, transferring, and fusion with picoliter precision.
Yunxia Zhang - One of the best experts on this subject based on the ideXlab platform.
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sequential operation droplet array an automated microfluidic platform for picoliter scale liquid handling analysis and screening
Analytical Chemistry, 2013Co-Authors: Yunxia Zhang, Qun FangAbstract:This contribution describes a sequential operation droplet array (SODA) system, a fully automated droplet-based microfluidic system capable of performing picoliter-scale liquid manipulation, analysis, and screening. The SODA system was built using a tapered capillary-syringe Pump Module and a two-dimensional (2D) oil-covered droplet array installed on an x–y–z translation stage. With the system, we developed a novel picoliter-scale droplet depositing technique for forming a 2D picoliter-droplet array. On this basis, an automated droplet manipulation method with picoliter precision was established using the programmable combination of the capillary-based liquid aspirating–depositing and the moving of the oil-covered droplet array, the so-called “aspirating–depositing–moving” (ADM) method. Differing from the previously reported droplet systems based on microchips, microcapillaries, or digital microfluidics, this method can achieve complete and flexible droplet manipulations, including droplet assembling, ge...
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sequential operation droplet array an automated microfluidic platform for picoliter scale liquid handling analysis and screening
Analytical Chemistry, 2013Co-Authors: Ying Zhu, Yunxia Zhang, Longfei Cai, Qun FangAbstract:This contribution describes a sequential operation droplet array (SODA) system, a fully automated droplet-based microfluidic system capable of performing picoliter-scale liquid manipulation, analysis, and screening. The SODA system was built using a tapered capillary-syringe Pump Module and a two-dimensional (2D) oil-covered droplet array installed on an x-y-z translation stage. With the system, we developed a novel picoliter-scale droplet depositing technique for forming a 2D picoliter-droplet array. On this basis, an automated droplet manipulation method with picoliter precision was established using the programmable combination of the capillary-based liquid aspirating-depositing and the moving of the oil-covered droplet array, the so-called "aspirating-depositing-moving" (ADM) method. Differing from the previously reported droplet systems based on microchips, microcapillaries, or digital microfluidics, this method can achieve complete and flexible droplet manipulations, including droplet assembling, generation, indexing, transferring, splitting, and fusion in the picoliter range, endowing the present system with ultralow sample/reagent consumptions and substantial versatility in analysis and screening for multiple different samples. To demonstrate its feasibility and versatility, we applied the SODA system in multiple experiments required in drug screening, including the screening of inhibitors for capases-1 from a chemical library, the measurement of IC50 values for the identified inhibitors, and the screening of the synergistic effect of multiple inhibitors. In the experiments, the consumptions of samples and reagents are only 60-180 pL for each droplet microreactor, which are commonly 3-5 orders of magnitude lower than those of conventional multiwell plate systems, and 1-2 orders of magnitude lower than other droplet-based microfluidic systems for multiple sample screening. The ability of the SODA system in performing complicated and multistep droplet manipulations was further demonstrated in the serial dilution of nanoliter-scale inhibitor droplets with concentrations spanning 6 orders of magnitude for IC50 profiling, which includes droplet generation, indexing, splitting, transferring, and fusion with picoliter precision.
Francesco Meli - One of the best experts on this subject based on the ideXlab platform.
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New Raman Pump Module for reducing Pump-signal four-wave-mixing interaction in co-Pumped distributed Raman amplifiers
Journal of Lightwave Technology, 2003Co-Authors: F. De Pasquale, Francesco MeliAbstract:We propose a new Raman Pump Module that effectively reduces four-wave-mixing (FWM) nonlinear interaction among longitudinal Pump modes and wavelength-division-multiplexed (WDM) signals in co-Pumped distributed Raman amplifiers based on nonzero dispersion-shifted fibers (NZ-DSFs). Theory and experiments confirm that the proposed Pump Module structure, based on polarization- and wavelength-multiplexed Fabry-Perot lasers, allows distributed amplification with high co-propagating on-off Raman gain, providing a key technology for all-Raman-based dense-WDM ultralong-haul transmission.
Ying Zhu - One of the best experts on this subject based on the ideXlab platform.
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sequential operation droplet array an automated microfluidic platform for picoliter scale liquid handling analysis and screening
Analytical Chemistry, 2013Co-Authors: Ying Zhu, Yunxia Zhang, Longfei Cai, Qun FangAbstract:This contribution describes a sequential operation droplet array (SODA) system, a fully automated droplet-based microfluidic system capable of performing picoliter-scale liquid manipulation, analysis, and screening. The SODA system was built using a tapered capillary-syringe Pump Module and a two-dimensional (2D) oil-covered droplet array installed on an x-y-z translation stage. With the system, we developed a novel picoliter-scale droplet depositing technique for forming a 2D picoliter-droplet array. On this basis, an automated droplet manipulation method with picoliter precision was established using the programmable combination of the capillary-based liquid aspirating-depositing and the moving of the oil-covered droplet array, the so-called "aspirating-depositing-moving" (ADM) method. Differing from the previously reported droplet systems based on microchips, microcapillaries, or digital microfluidics, this method can achieve complete and flexible droplet manipulations, including droplet assembling, generation, indexing, transferring, splitting, and fusion in the picoliter range, endowing the present system with ultralow sample/reagent consumptions and substantial versatility in analysis and screening for multiple different samples. To demonstrate its feasibility and versatility, we applied the SODA system in multiple experiments required in drug screening, including the screening of inhibitors for capases-1 from a chemical library, the measurement of IC50 values for the identified inhibitors, and the screening of the synergistic effect of multiple inhibitors. In the experiments, the consumptions of samples and reagents are only 60-180 pL for each droplet microreactor, which are commonly 3-5 orders of magnitude lower than those of conventional multiwell plate systems, and 1-2 orders of magnitude lower than other droplet-based microfluidic systems for multiple sample screening. The ability of the SODA system in performing complicated and multistep droplet manipulations was further demonstrated in the serial dilution of nanoliter-scale inhibitor droplets with concentrations spanning 6 orders of magnitude for IC50 profiling, which includes droplet generation, indexing, splitting, transferring, and fusion with picoliter precision.
Aldo Antognini - One of the best experts on this subject based on the ideXlab platform.
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thin disk laser Pump schemes for large number of passes and moderate Pump source quality
Applied Optics, 2015Co-Authors: Karsten Schuhmann, T W Hansch, K Kirch, A Knecht, F Kottmann, Randolf Pohl, D Taqqu, Aldo AntogniniAbstract:Thin-disk laser Pump layouts yielding an increased number of passes for a given Pump Module size and Pump source quality are proposed. These layouts result from a general scheme based on merging two simpler Pump optics arrangements. Some peculiar examples can be realized by adapting standard, commercially available Pump optics with an additional mirror pair. More Pump passes yield better efficiency, opening the way for the usage of active materials with low absorption. In a standard multipass Pump design, scaling of the number of beam passes brings about an increase in the overall size of the optical arrangement or an increase in the Pump source quality requirements. Such increases are minimized in our scheme, making them eligible for industrial applications.
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thin disk laser Pump schemes for large number of passes and moderate Pump source quality
arXiv: Optics, 2015Co-Authors: Karsten Schuhmann, T W Hansch, K Kirch, A Knecht, F Kottmann, Randolf Pohl, D Taqqu, Aldo AntogniniAbstract:Novel thin-disk laser Pump layouts are proposed yielding an increased number of passes for a given Pump Module size and Pump source quality. These novel layouts result from a general scheme which bases on merging two simpler Pump optics arrangements. Some peculiar examples can be realized by adapting standard commercially available Pump optics simply by intro ducing an additional mirror-pair. More Pump passes yield better efficiency, opening the way for usage of active materials with low absorption. In a standard multi-pass Pump design, scaling of the number of beam passes brings ab out an increase of the overall size of the optical arrangement or an increase of the Pump source quality requirements. Such increases are minimized in our scheme, making them eligible for industrial applications
