The Experts below are selected from a list of 207 Experts worldwide ranked by ideXlab platform
Chengliang Gong - One of the best experts on this subject based on the ideXlab platform.
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effects of bmkit 3 r gene transfer on Pupal Development of bombyx mori linnaeus using a gal4 uas binary transgenic system
Journal of Agricultural and Food Chemistry, 2012Co-Authors: Haokun Zhang, Xiaorong Zhang, Xiaojuan Wang, Chengliang GongAbstract:The Pupal stage of the silkworm Bombyx mori Linnaeus lasts for approximately two weeks. However, prolongation of Pupal duration would reduce the labor required to process and dry fresh cocoons. This study investigated the effects of BmKIT3R gene (from the Chinese scorpion Buthus martensii Karsch) transfer on the Pupal Development of B. mori using a Gal4/UAS binary transgenic system. Gal4 driven by a pupa-specific promoter BmWCP4 (from a B. mori wing-cuticle protein gene) or PDP (from a B. mori cocoonase gene), and BmKIT3R driven by a UAS cis-acting element were used to construct novel piggyBac-derived plasmids containing a neomycin-resistance gene (neo) controlled by the Bombyx mori nucleopolyhedrovirus (BmNPV) ie-1 (immediate-early gene) promoter and a green fluorescent protein gene (gfp) under the control of the B. mori actin 3 (A3) promoter. The vector was transferred into silkworm eggs by sperm-mediated gene transfer. Transgenic silkworms were produced after screening for neo and gfp genes, and gene t...
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Effects of BmKIT 3 R gene transfer on Pupal Development of Bombyx mori Linnaeus using a Gal4/UAS binary transgenic system.
Journal of Agricultural and Food Chemistry, 2012Co-Authors: Haokun Zhang, Xiaorong Zhang, Xiaojuan Wang, Chengliang GongAbstract:The Pupal stage of the silkworm Bombyx mori Linnaeus lasts for approximately two weeks. However, prolongation of Pupal duration would reduce the labor required to process and dry fresh cocoons. This study investigated the effects of BmKIT3R gene (from the Chinese scorpion Buthus martensii Karsch) transfer on the Pupal Development of B. mori using a Gal4/UAS binary transgenic system. Gal4 driven by a pupa-specific promoter BmWCP4 (from a B. mori wing-cuticle protein gene) or PDP (from a B. mori cocoonase gene), and BmKIT3R driven by a UAS cis-acting element were used to construct novel piggyBac-derived plasmids containing a neomycin-resistance gene (neo) controlled by the Bombyx mori nucleopolyhedrovirus (BmNPV) ie-1 (immediate-early gene) promoter and a green fluorescent protein gene (gfp) under the control of the B. mori actin 3 (A3) promoter. The vector was transferred into silkworm eggs by sperm-mediated gene transfer. Transgenic silkworms were produced after screening for neo and gfp genes, and gene t...
Jose F De Celis - One of the best experts on this subject based on the ideXlab platform.
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regulation of decapentaplegic expression during drosophila wing veins Pupal Development
Mechanisms of Development, 2006Co-Authors: Sol Sotillos, Jose F De CelisAbstract:Abstract The differentiation of veins in the Drosophila wing relies on localised expression of decapentaplegic (dpp) in pro-vein territories during Pupal Development. The expression of dpp in the Pupal veins requires the integrity of the shortvein region (shv), localised 5′ to the coding region. It is likely that this DNA integrates positive and negative regulatory signals directing dpp transcription during Pupal Development. Here, we identify a minimal 0.9 kb fragment giving localised expression in the vein L5 and a 0.5 kb fragment giving expression in all longitudinal veins. Using a combination of in vivo expression of reporter genes regulated by shv sequences, in vitro binding assays and sequence comparisons between the shv region of different Drosophila species, we found binding sites for the vein-specific transciption factors Araucan, Knirps and Ventral veinless, as well as binding sites for the Dpp pathway effectors Mad and Med. We conclude that conserved vein-specific enhancers regulated by transcription factors expressed in individual veins collaborate with general vein and intervein regulators to establish and maintain the expression of dpp confined to the veins during Pupal Development.
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interactions between the notch egfr and decapentaplegic signaling pathways regulate vein differentiation during drosophila Pupal wing Development
Developmental Dynamics, 2005Co-Authors: Sol Sotillos, Jose F De CelisAbstract:The formation of longitudinal veins in the Drosophila wing involves cell interactions mediated by the conserved signaling pathways Decapentaplegic (Dpp), Notch, and epidermal growth factor receptor (EGFR). Interactions between Notch and EGFR taking place in the wing disc divide each vein into a central domain, where EGFR is active, and two boundary domains where Notch is active. The expression of decapentaplegic (dpp) is activated in the veins during Pupal Development, and we have generated Gal4 drivers using the regulatory region that drives dpp expression at this stage. By using these drivers, we studied the relationships between the Notch, EGFR, and Dpp signaling pathways that occur during Pupal Development. Our results indicate that the interactions between EGFR and Notch initiated in the imaginal disc are maintained throughout Pupal Development and contribute to determine the places where dpp is expressed. Once dpp expression is initiated, Dpp and EGFR activities in the provein maintain each other and, in cooperation, determine vein cell differentiation. Developmental Dynamics 232:738–752, 2005. © 2005 Wiley-Liss, Inc.
G. A. Vale - One of the best experts on this subject based on the ideXlab platform.
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models for the rates of Pupal Development fat consumption and mortality in tsetse glossina spp
Bulletin of Entomological Research, 2020Co-Authors: John W. Hargrove, G. A. ValeAbstract:: Environmental temperature is an important driver of the population dynamics of tsetse (Glossina spp) because the fly's immature stages are particularly vulnerable to temperatures (T) outside the range T = 16-32°C. Laboratory experiments carried out 50 years ago provide extensive measures of temperature-dependent rates of Development, fat consumption and mortality in tsetse pupae. We improve on the models originally fitted to these data, providing better parameter estimates for use in population modelling. A composite function accurately models rates of Pupal Development for T = 8-32°C. Pupal duration can be estimated by summing the temperature-dependent daily percentage of Development completed. Fat consumption is modelled as a logistic function of temperature; the total fat consumed during Pupal Development takes a minimum for T ≈ 25°C. Pupae experiencing constant temperatures <16°C exhaust their fat reserves before they complete Development. At high temperatures, direct effects kill the pupae before fat stores are exhausted. The relationship between Pupal mortality and temperature is well described by the sum of two exponential functions. Summing daily mortality rates over the whole Pupal period does not reliably predict overall mortality. Mortality is more strongly correlated with the mean temperature experienced over Pupal life or, for T ≤ 30°C, the fat consumption during this period. The new results will be particularly useful in the construction of various models for tsetse population dynamics, and will have particular relevance for agent-based models where the lives of individual tsetse are simulated using a daily time step.
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Models for the rates of Pupal Development, fat consumption and mortality in tsetse (Glossina spp).
Bulletin of Entomological Research, 2019Co-Authors: John W. Hargrove, G. A. ValeAbstract:Environmental temperature is an important driver of the population dynamics of tsetse ( Glossina spp) because the fly's immature stages are particularly vulnerable to temperatures ( T ) outside the range T = 16–32°C. Laboratory experiments carried out 50 years ago provide extensive measures of temperature-dependent rates of Development, fat consumption and mortality in tsetse pupae. We improve on the models originally fitted to these data, providing better parameter estimates for use in population modelling. A composite function accurately models rates of Pupal Development for T = 8–32°C. Pupal duration can be estimated by summing the temperature-dependent daily percentage of Development completed. Fat consumption is modelled as a logistic function of temperature; the total fat consumed during Pupal Development takes a minimum for T ≈ 25°C. Pupae experiencing constant temperatures T ≤ 30°C, the fat consumption during this period. The new results will be particularly useful in the construction of various models for tsetse population dynamics, and will have particular relevance for agent-based models where the lives of individual tsetse are simulated using a daily time step.
Haokun Zhang - One of the best experts on this subject based on the ideXlab platform.
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effects of bmkit 3 r gene transfer on Pupal Development of bombyx mori linnaeus using a gal4 uas binary transgenic system
Journal of Agricultural and Food Chemistry, 2012Co-Authors: Haokun Zhang, Xiaorong Zhang, Xiaojuan Wang, Chengliang GongAbstract:The Pupal stage of the silkworm Bombyx mori Linnaeus lasts for approximately two weeks. However, prolongation of Pupal duration would reduce the labor required to process and dry fresh cocoons. This study investigated the effects of BmKIT3R gene (from the Chinese scorpion Buthus martensii Karsch) transfer on the Pupal Development of B. mori using a Gal4/UAS binary transgenic system. Gal4 driven by a pupa-specific promoter BmWCP4 (from a B. mori wing-cuticle protein gene) or PDP (from a B. mori cocoonase gene), and BmKIT3R driven by a UAS cis-acting element were used to construct novel piggyBac-derived plasmids containing a neomycin-resistance gene (neo) controlled by the Bombyx mori nucleopolyhedrovirus (BmNPV) ie-1 (immediate-early gene) promoter and a green fluorescent protein gene (gfp) under the control of the B. mori actin 3 (A3) promoter. The vector was transferred into silkworm eggs by sperm-mediated gene transfer. Transgenic silkworms were produced after screening for neo and gfp genes, and gene t...
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Effects of BmKIT 3 R gene transfer on Pupal Development of Bombyx mori Linnaeus using a Gal4/UAS binary transgenic system.
Journal of Agricultural and Food Chemistry, 2012Co-Authors: Haokun Zhang, Xiaorong Zhang, Xiaojuan Wang, Chengliang GongAbstract:The Pupal stage of the silkworm Bombyx mori Linnaeus lasts for approximately two weeks. However, prolongation of Pupal duration would reduce the labor required to process and dry fresh cocoons. This study investigated the effects of BmKIT3R gene (from the Chinese scorpion Buthus martensii Karsch) transfer on the Pupal Development of B. mori using a Gal4/UAS binary transgenic system. Gal4 driven by a pupa-specific promoter BmWCP4 (from a B. mori wing-cuticle protein gene) or PDP (from a B. mori cocoonase gene), and BmKIT3R driven by a UAS cis-acting element were used to construct novel piggyBac-derived plasmids containing a neomycin-resistance gene (neo) controlled by the Bombyx mori nucleopolyhedrovirus (BmNPV) ie-1 (immediate-early gene) promoter and a green fluorescent protein gene (gfp) under the control of the B. mori actin 3 (A3) promoter. The vector was transferred into silkworm eggs by sperm-mediated gene transfer. Transgenic silkworms were produced after screening for neo and gfp genes, and gene t...
Weihua Xu - One of the best experts on this subject based on the ideXlab platform.
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wnt β catenin signaling regulates helicoverpa armigera Pupal Development by up regulating c myc and ap 4
Insect Biochemistry and Molecular Biology, 2014Co-Authors: Wei Chen, Weihua XuAbstract:Seasonally changing environmental conditions perceived by insect brains can be converted into hormonal signals that prompt insects to make a decision to develop or enter Developmental arrest (diapause). Diapause is a complex physiological response, and many signaling pathways may participate in its regulation. However, little is known about these regulatory pathways. In this study, we cloned four genes related to the Wnt/β-catenin signaling pathway from Helicoverpa armigera, a Pupal diapause species. Western blotting shows that expression of Har-Wnt1, Har-β-catenin, and Har-c-Myc are higher in non-diapause Pupal brains than in diapause-destined brains. Har-Wnt1 can promote the accumulation of Har-β-catenin in the nucleus, and Har-β-catenin in turn increases the expression of Har-c-Myc. The blockage of Wnt/β-catenin signaling by the inhibitor XAV939 significantly down-regulates Har-β-catenin and Har-c-Myc expression and delays Pupal Development, suggesting that the Wnt/β-catenin pathway functions in insect Development. Furthermore, Har-c-Myc binds to the promoter of Har-AP-4 and regulates its expression. It has been reported that Har-AP-4 activates diapause hormone (DH) expression and that DH up-regulates the growth hormone ecdysteroid for Pupal Development. Thus, Pupal Development is regulated by Wnt/β-catenin signaling through the pathway Wnt-β-catenin-c-Myc-AP-4-DH-ecdysteroid. In contrast, the down-regulation of Wnt/β-catenin signaling is likely to induce insects to enter diapause.
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Wnt/β-catenin signaling regulates Helicoverpa armigera Pupal Development by up-regulating c-Myc and AP-4.
Insect Biochemistry and Molecular Biology, 2014Co-Authors: Wei Chen, Weihua XuAbstract:Seasonally changing environmental conditions perceived by insect brains can be converted into hormonal signals that prompt insects to make a decision to develop or enter Developmental arrest (diapause). Diapause is a complex physiological response, and many signaling pathways may participate in its regulation. However, little is known about these regulatory pathways. In this study, we cloned four genes related to the Wnt/β-catenin signaling pathway from Helicoverpa armigera, a Pupal diapause species. Western blotting shows that expression of Har-Wnt1, Har-β-catenin, and Har-c-Myc are higher in non-diapause Pupal brains than in diapause-destined brains. Har-Wnt1 can promote the accumulation of Har-β-catenin in the nucleus, and Har-β-catenin in turn increases the expression of Har-c-Myc. The blockage of Wnt/β-catenin signaling by the inhibitor XAV939 significantly down-regulates Har-β-catenin and Har-c-Myc expression and delays Pupal Development, suggesting that the Wnt/β-catenin pathway functions in insect Development. Furthermore, Har-c-Myc binds to the promoter of Har-AP-4 and regulates its expression. It has been reported that Har-AP-4 activates diapause hormone (DH) expression and that DH up-regulates the growth hormone ecdysteroid for Pupal Development. Thus, Pupal Development is regulated by Wnt/β-catenin signaling through the pathway Wnt-β-catenin-c-Myc-AP-4-DH-ecdysteroid. In contrast, the down-regulation of Wnt/β-catenin signaling is likely to induce insects to enter diapause.
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effect of the brain and suboesophageal ganglion on Pupal Development in helicoverpa armigera through regulation of fxprlamide neuropeptides
Regulatory Peptides, 2003Co-Authors: Tianyi Zhang, Weihua Xu, Qirui ZhangAbstract:Abstract Recent studies in Helicoverpa armigera report a novel role for diapause hormone (DH), pheromone biosynthesis activating neuropeptide (PBAN) and three other FXPRLamide neuropeptides secreted from suboesophageal ganglion (SG) in terminating Pupal diapause. In the present paper, we investigated the role of these five FXPRLamide family neuropeptides on Pupal Development. Although removal of SG could not make nondiapause-destined pupae enter diapause-like status, it did make them eclose approximately 0.6–1.2 days later when compared with the controls. The results of competitive ELISAs showed a high level of FXPRLamide titer in the hemolymph of the SG-removed pupae and this may be due to the expression of the DH-PBAN gene in tissues other than SG. DH-PBAN mRNA and peptides were also detected in the thoracic ganglia (TGs) by RT-PCR and immunocytochemistry. The expression of DH-PBAN gene in the TGs of the SG-removed pupae is significantly higher than that in normal pupae by quantitative PCR and immunocytochemistry. Decerebration experiments proved that the decerebrated pupae could enter diapause-like status through down-regulation of FXPRLamide titer in hemolymph. Our studies confirm that the brain plays an important role in the determination of Pupal Development by regulating the synthesis and release of FXPRLamide neuropeptides in H. armigera. Thus, the function of FXPRLamide peptides in H. armigera is closely correlated with Pupal Development.
