Pyrolobus

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Karl O. Stetter - One of the best experts on this subject based on the ideXlab platform.

  • Discovery of hyperthermophilic microorganisms.
    Methods in enzymology, 2001
    Co-Authors: Robert Huber, Karl O. Stetter
    Abstract:

    Publisher Summary A variety of hyperthermophilic Archaea and Bacteria have been isolated from water-containing terrestrial, subterranean, and submarine high-temperature ecosystems. Hyperthermophiles grow fastest at temperatures between 80° and 106°. In contrast to moderate thermophiles, they are unable to propagate below about 60 °. Pyrolobus fumarii , the most extreme hyperthermophile, is even unable to grow below 90° and exhibits the highest growth temperature observed of all at 113°. Hyperthermophiles are very divergent, both in terms of their phylogeny and physiological and biochemical properties. Because of their metabolic flexibility and their outstanding heat resistance, hyperthermophiles are as interesting for basic research as they are for biotechnological applications. So far, hyperthermophiles have been isolated mainly from water-containing terrestrial and marine high-temperature areas, where they form microbial communities. On land, natural biotopes of hyperthermophiles are water-containing areas, such as hot springs and solfataric fields, with a wide range of pH values (pH 0.5-9.0) and usually low salinity (0.1-0.5%). Marine biotopes of hyperthermophiles are shallow hydrothermal systems, abyssal hot vents—black smokers—and active seamounts, such as Teahicya and Macdonald in the Tahiti area, Polynesia. These biotopes are characterized by high concentrations of salt (about 3%) and pH values that are slightly acidic to slightly alkaline (pH 5.0-8.5).

  • Posttranscriptional modification of transfer RNA in the submarine hyperthermophile Pyrolobus fumarii
    Nucleic Acids Symposium Series, 2000
    Co-Authors: James A. Mccloskey, Xiao-hui Liu, Pamela F. Crain, Eveline Bruenger, Rebecca Guymon, Takeshi Hashizume, Karl O. Stetter
    Abstract:

    In the RNA of hyperthermoph iles, which grow optimally between 80 °C and 106 °C, posttranscriptional modification has been identified as a leading mechanism of structural stabilization. Particularly in the Archaeal evolutionary domain these modifications are expressed as a structurally diverse array of modification motifs, many of which include ribose methylation. Using mass spectrometric techniques we have examined the posttranscriptional modifications in unfractionated tRNA from the remarkable organism Pyrolobus fumarii, which grows optimally at 106°C, but up to 113°C (Blochl et al. (1997), Extremophiles, 1, 14-21). Twenty-six modified nucleosides were detected, 11 of which are methylated in ribose. A new RNA nucleoside, l,2'-O-dimethy!guanosine (m'Gm) was characterized and the structure confirmed by chemical synthesis.

  • Extremophiles and their adaptation to hot environments.
    FEBS Letters, 1999
    Co-Authors: Karl O. Stetter
    Abstract:

    Water-containing terrestrial, subterranean and submarine high temperature areas harbor a variety of hyperthermophilic bacteria and archaea which are able to grow optimally above 80°C. Hyperthermophiles are adapted to hot environments by their physiological and nutritional requirements. As a consequence, cell components like proteins, nucleic acids and membranes have to be stable and even function best at temperatures around 100°C. The chemolithoautotrophic archaeon Pyrolobus fumarii is able to grow at 113°C and, therefore, represents the upper temperature border of life. For the first time, (vegetative) cultures of Pyrolobus and Pyrodictium are able to survive autoclaving.

  • Extremophiles and their adaptation to hot environments.
    FEBS letters, 1999
    Co-Authors: Karl O. Stetter
    Abstract:

    Water-containing terrestrial, subterranean and submarine high temperature areas harbor a variety of hyperthermophilic bacteria and archaea which are able to grow optimally above 80 degrees C. Hyperthermophiles are adapted to hot environments by their physiological and nutritional requirements. As a consequence, cell components like proteins, nucleic acids and membranes have to be stable and even function best at temperatures around 100 degrees C. The chemolithoautotrophic archaeon Pyrolobus fumarii is able to grow at 113 degrees C and, therefore, represents the upper temperature border of life. For the first time, (vegetative) cultures of Pyrolobus and Pyrodictium are able to survive autoclaving.

Lynne Goodwin - One of the best experts on this subject based on the ideXlab platform.

  • Standards in Genomic Sciences (2011) 4:381-392 DOI:10.4056/sigs.2014648 The Genomic Standards Consortium Complete
    2016
    Co-Authors: Iain Anderson, Nancy Hammon, Roxanne Tapia, Markus Göker, Jan-fang Cheng, Susan Lucas, Lynne Goodwin, Matt Nolan, Cliff Han, Marcel Huntemann
    Abstract:

    genome sequence of the hyperthermophilic chemolithoautotroph Pyrolobus fumarii type strain (1AT

  • Complete genome sequence of the hyperthermophilic chemolithoautotroph Pyrolobus fumarii
    Standards in Genomic Sciences, 2011
    Co-Authors: Iain Anderson, Nancy Hammon, Roxanne Tapia, Markus Göker, Jan-fang Cheng, Susan Lucas, Shweta Deshpande, Matt Nolan, Lynne Goodwin
    Abstract:

    Pyrolobus fumarii Blochl et al. 1997 is the type species of the genus Pyrolobus, which belongs to the crenarchaeal family Pyrodictiaceae. The species is a facultatively microaerophilic non-motile crenarchaeon. It is of interest because of its isolated phylogenetic location in the tree of life and because it is a hyperthermophilic chemolithoautotroph known as the primary producer of organic matter at deep-sea hydrothermal vents. P. fumarii exhibits currently the highest optimal growth temperature of all life forms on earth (106°C). This is the first completed genome sequence of a member of the genus Pyrolobus to be published and only the second genome sequence from a member of the family Pyrodictiaceae. Although Diversa Corporation announced the completion of sequencing of the P. fumarii genome on September 25, 2001, this sequence was never released to the public. The 1,843,267 bp long genome with its 1,986 protein-coding and 52 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

  • complete genome sequence of the hyperthermophilic chemolithoautotroph Pyrolobus fumarii type strain 1a t
    Standards in Genomic Sciences, 2011
    Co-Authors: Iain Anderson, Nancy Hammon, Roxanne Tapia, Markus Göker, Jan-fang Cheng, Susan Lucas, Shweta Deshpande, Matt Nolan, Lynne Goodwin
    Abstract:

    Pyrolobus fumarii Blochl et al. 1997 is the type species of the genus Pyrolobus, which belongs to the crenarchaeal family Pyrodictiaceae. The species is a facultatively microaerophilic non-motile crenarchaeon. It is of interest because of its isolated phylogenetic location in the tree of life and because it is a hyperthermophilic chemolithoautotroph known as the primary producer of organic matter at deep-sea hydrothermal vents. P. fumarii exhibits currently the highest optimal growth temperature of all life forms on earth (106°C). This is the first completed genome sequence of a member of the genus Pyrolobus to be published and only the second genome sequence from a member of the family Pyrodictiaceae. Although Diversa Corporation announced the completion of sequencing of the P. fumarii genome on September 25, 2001, this sequence was never released to the public. The 1,843,267 bp long genome with its 1,986 protein-coding and 52 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

  • complete genome sequence of the hyperthermophilic chemolithoautotroph Pyrolobus fumarii type strain 1a
    Standards in Genomic Sciences, 2011
    Co-Authors: Iain Anderson, Nancy Hammon, Roxanne Tapia, Markus Göker, Jan-fang Cheng, Susan Lucas, Lynne Goodwin, Shweta Deshpande, Matt Nolan, Sam Pitluck
    Abstract:

    Pyrolobus fumarii Blochl et al. 1997 is the type species of the genus Pyrolobus, which belongs to the crenarchaeal family Pyrodictiaceae. The species is a facultatively microaerophilic non-motile crenarchaeon. It is of interest because of its isolated phylogenetic location in the tree of life and because it is a hyperthermophilic chemolithoautotroph known as the primary producer of organic matter at deep-sea hydrothermal vents. P. fumarii exhibits currently the highest optimal growth temperature of all life forms on earth (106°C). This is the first completed genome sequence of a member of the genus Pyrolobus to be published and only the second genome sequence from a member of the family Pyrodictiaceae. Although Diversa Corporation announced the completion of sequencing of the P. fumarii genome on September 25, 2001, this sequence was never released to the public. The 1,843,267 bp long genome with its 1,986 protein-coding and 52 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

Iain Anderson - One of the best experts on this subject based on the ideXlab platform.

  • Standards in Genomic Sciences (2011) 4:381-392 DOI:10.4056/sigs.2014648 The Genomic Standards Consortium Complete
    2016
    Co-Authors: Iain Anderson, Nancy Hammon, Roxanne Tapia, Markus Göker, Jan-fang Cheng, Susan Lucas, Lynne Goodwin, Matt Nolan, Cliff Han, Marcel Huntemann
    Abstract:

    genome sequence of the hyperthermophilic chemolithoautotroph Pyrolobus fumarii type strain (1AT

  • Complete genome sequence of the hyperthermophilic chemolithoautotroph Pyrolobus fumarii
    Standards in Genomic Sciences, 2011
    Co-Authors: Iain Anderson, Nancy Hammon, Roxanne Tapia, Markus Göker, Jan-fang Cheng, Susan Lucas, Shweta Deshpande, Matt Nolan, Lynne Goodwin
    Abstract:

    Pyrolobus fumarii Blochl et al. 1997 is the type species of the genus Pyrolobus, which belongs to the crenarchaeal family Pyrodictiaceae. The species is a facultatively microaerophilic non-motile crenarchaeon. It is of interest because of its isolated phylogenetic location in the tree of life and because it is a hyperthermophilic chemolithoautotroph known as the primary producer of organic matter at deep-sea hydrothermal vents. P. fumarii exhibits currently the highest optimal growth temperature of all life forms on earth (106°C). This is the first completed genome sequence of a member of the genus Pyrolobus to be published and only the second genome sequence from a member of the family Pyrodictiaceae. Although Diversa Corporation announced the completion of sequencing of the P. fumarii genome on September 25, 2001, this sequence was never released to the public. The 1,843,267 bp long genome with its 1,986 protein-coding and 52 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

  • complete genome sequence of the hyperthermophilic chemolithoautotroph Pyrolobus fumarii type strain 1a t
    Standards in Genomic Sciences, 2011
    Co-Authors: Iain Anderson, Nancy Hammon, Roxanne Tapia, Markus Göker, Jan-fang Cheng, Susan Lucas, Shweta Deshpande, Matt Nolan, Lynne Goodwin
    Abstract:

    Pyrolobus fumarii Blochl et al. 1997 is the type species of the genus Pyrolobus, which belongs to the crenarchaeal family Pyrodictiaceae. The species is a facultatively microaerophilic non-motile crenarchaeon. It is of interest because of its isolated phylogenetic location in the tree of life and because it is a hyperthermophilic chemolithoautotroph known as the primary producer of organic matter at deep-sea hydrothermal vents. P. fumarii exhibits currently the highest optimal growth temperature of all life forms on earth (106°C). This is the first completed genome sequence of a member of the genus Pyrolobus to be published and only the second genome sequence from a member of the family Pyrodictiaceae. Although Diversa Corporation announced the completion of sequencing of the P. fumarii genome on September 25, 2001, this sequence was never released to the public. The 1,843,267 bp long genome with its 1,986 protein-coding and 52 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

  • complete genome sequence of the hyperthermophilic chemolithoautotroph Pyrolobus fumarii type strain 1a
    Standards in Genomic Sciences, 2011
    Co-Authors: Iain Anderson, Nancy Hammon, Roxanne Tapia, Markus Göker, Jan-fang Cheng, Susan Lucas, Lynne Goodwin, Shweta Deshpande, Matt Nolan, Sam Pitluck
    Abstract:

    Pyrolobus fumarii Blochl et al. 1997 is the type species of the genus Pyrolobus, which belongs to the crenarchaeal family Pyrodictiaceae. The species is a facultatively microaerophilic non-motile crenarchaeon. It is of interest because of its isolated phylogenetic location in the tree of life and because it is a hyperthermophilic chemolithoautotroph known as the primary producer of organic matter at deep-sea hydrothermal vents. P. fumarii exhibits currently the highest optimal growth temperature of all life forms on earth (106°C). This is the first completed genome sequence of a member of the genus Pyrolobus to be published and only the second genome sequence from a member of the family Pyrodictiaceae. Although Diversa Corporation announced the completion of sequencing of the P. fumarii genome on September 25, 2001, this sequence was never released to the public. The 1,843,267 bp long genome with its 1,986 protein-coding and 52 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

Roxanne Tapia - One of the best experts on this subject based on the ideXlab platform.

  • Standards in Genomic Sciences (2011) 4:381-392 DOI:10.4056/sigs.2014648 The Genomic Standards Consortium Complete
    2016
    Co-Authors: Iain Anderson, Nancy Hammon, Roxanne Tapia, Markus Göker, Jan-fang Cheng, Susan Lucas, Lynne Goodwin, Matt Nolan, Cliff Han, Marcel Huntemann
    Abstract:

    genome sequence of the hyperthermophilic chemolithoautotroph Pyrolobus fumarii type strain (1AT

  • Complete genome sequence of the hyperthermophilic chemolithoautotroph Pyrolobus fumarii
    Standards in Genomic Sciences, 2011
    Co-Authors: Iain Anderson, Nancy Hammon, Roxanne Tapia, Markus Göker, Jan-fang Cheng, Susan Lucas, Shweta Deshpande, Matt Nolan, Lynne Goodwin
    Abstract:

    Pyrolobus fumarii Blochl et al. 1997 is the type species of the genus Pyrolobus, which belongs to the crenarchaeal family Pyrodictiaceae. The species is a facultatively microaerophilic non-motile crenarchaeon. It is of interest because of its isolated phylogenetic location in the tree of life and because it is a hyperthermophilic chemolithoautotroph known as the primary producer of organic matter at deep-sea hydrothermal vents. P. fumarii exhibits currently the highest optimal growth temperature of all life forms on earth (106°C). This is the first completed genome sequence of a member of the genus Pyrolobus to be published and only the second genome sequence from a member of the family Pyrodictiaceae. Although Diversa Corporation announced the completion of sequencing of the P. fumarii genome on September 25, 2001, this sequence was never released to the public. The 1,843,267 bp long genome with its 1,986 protein-coding and 52 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

  • complete genome sequence of the hyperthermophilic chemolithoautotroph Pyrolobus fumarii type strain 1a t
    Standards in Genomic Sciences, 2011
    Co-Authors: Iain Anderson, Nancy Hammon, Roxanne Tapia, Markus Göker, Jan-fang Cheng, Susan Lucas, Shweta Deshpande, Matt Nolan, Lynne Goodwin
    Abstract:

    Pyrolobus fumarii Blochl et al. 1997 is the type species of the genus Pyrolobus, which belongs to the crenarchaeal family Pyrodictiaceae. The species is a facultatively microaerophilic non-motile crenarchaeon. It is of interest because of its isolated phylogenetic location in the tree of life and because it is a hyperthermophilic chemolithoautotroph known as the primary producer of organic matter at deep-sea hydrothermal vents. P. fumarii exhibits currently the highest optimal growth temperature of all life forms on earth (106°C). This is the first completed genome sequence of a member of the genus Pyrolobus to be published and only the second genome sequence from a member of the family Pyrodictiaceae. Although Diversa Corporation announced the completion of sequencing of the P. fumarii genome on September 25, 2001, this sequence was never released to the public. The 1,843,267 bp long genome with its 1,986 protein-coding and 52 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

  • complete genome sequence of the hyperthermophilic chemolithoautotroph Pyrolobus fumarii type strain 1a
    Standards in Genomic Sciences, 2011
    Co-Authors: Iain Anderson, Nancy Hammon, Roxanne Tapia, Markus Göker, Jan-fang Cheng, Susan Lucas, Lynne Goodwin, Shweta Deshpande, Matt Nolan, Sam Pitluck
    Abstract:

    Pyrolobus fumarii Blochl et al. 1997 is the type species of the genus Pyrolobus, which belongs to the crenarchaeal family Pyrodictiaceae. The species is a facultatively microaerophilic non-motile crenarchaeon. It is of interest because of its isolated phylogenetic location in the tree of life and because it is a hyperthermophilic chemolithoautotroph known as the primary producer of organic matter at deep-sea hydrothermal vents. P. fumarii exhibits currently the highest optimal growth temperature of all life forms on earth (106°C). This is the first completed genome sequence of a member of the genus Pyrolobus to be published and only the second genome sequence from a member of the family Pyrodictiaceae. Although Diversa Corporation announced the completion of sequencing of the P. fumarii genome on September 25, 2001, this sequence was never released to the public. The 1,843,267 bp long genome with its 1,986 protein-coding and 52 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

Matt Nolan - One of the best experts on this subject based on the ideXlab platform.

  • Standards in Genomic Sciences (2011) 4:381-392 DOI:10.4056/sigs.2014648 The Genomic Standards Consortium Complete
    2016
    Co-Authors: Iain Anderson, Nancy Hammon, Roxanne Tapia, Markus Göker, Jan-fang Cheng, Susan Lucas, Lynne Goodwin, Matt Nolan, Cliff Han, Marcel Huntemann
    Abstract:

    genome sequence of the hyperthermophilic chemolithoautotroph Pyrolobus fumarii type strain (1AT

  • Complete genome sequence of the hyperthermophilic chemolithoautotroph Pyrolobus fumarii
    Standards in Genomic Sciences, 2011
    Co-Authors: Iain Anderson, Nancy Hammon, Roxanne Tapia, Markus Göker, Jan-fang Cheng, Susan Lucas, Shweta Deshpande, Matt Nolan, Lynne Goodwin
    Abstract:

    Pyrolobus fumarii Blochl et al. 1997 is the type species of the genus Pyrolobus, which belongs to the crenarchaeal family Pyrodictiaceae. The species is a facultatively microaerophilic non-motile crenarchaeon. It is of interest because of its isolated phylogenetic location in the tree of life and because it is a hyperthermophilic chemolithoautotroph known as the primary producer of organic matter at deep-sea hydrothermal vents. P. fumarii exhibits currently the highest optimal growth temperature of all life forms on earth (106°C). This is the first completed genome sequence of a member of the genus Pyrolobus to be published and only the second genome sequence from a member of the family Pyrodictiaceae. Although Diversa Corporation announced the completion of sequencing of the P. fumarii genome on September 25, 2001, this sequence was never released to the public. The 1,843,267 bp long genome with its 1,986 protein-coding and 52 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

  • complete genome sequence of the hyperthermophilic chemolithoautotroph Pyrolobus fumarii type strain 1a t
    Standards in Genomic Sciences, 2011
    Co-Authors: Iain Anderson, Nancy Hammon, Roxanne Tapia, Markus Göker, Jan-fang Cheng, Susan Lucas, Shweta Deshpande, Matt Nolan, Lynne Goodwin
    Abstract:

    Pyrolobus fumarii Blochl et al. 1997 is the type species of the genus Pyrolobus, which belongs to the crenarchaeal family Pyrodictiaceae. The species is a facultatively microaerophilic non-motile crenarchaeon. It is of interest because of its isolated phylogenetic location in the tree of life and because it is a hyperthermophilic chemolithoautotroph known as the primary producer of organic matter at deep-sea hydrothermal vents. P. fumarii exhibits currently the highest optimal growth temperature of all life forms on earth (106°C). This is the first completed genome sequence of a member of the genus Pyrolobus to be published and only the second genome sequence from a member of the family Pyrodictiaceae. Although Diversa Corporation announced the completion of sequencing of the P. fumarii genome on September 25, 2001, this sequence was never released to the public. The 1,843,267 bp long genome with its 1,986 protein-coding and 52 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

  • complete genome sequence of the hyperthermophilic chemolithoautotroph Pyrolobus fumarii type strain 1a
    Standards in Genomic Sciences, 2011
    Co-Authors: Iain Anderson, Nancy Hammon, Roxanne Tapia, Markus Göker, Jan-fang Cheng, Susan Lucas, Lynne Goodwin, Shweta Deshpande, Matt Nolan, Sam Pitluck
    Abstract:

    Pyrolobus fumarii Blochl et al. 1997 is the type species of the genus Pyrolobus, which belongs to the crenarchaeal family Pyrodictiaceae. The species is a facultatively microaerophilic non-motile crenarchaeon. It is of interest because of its isolated phylogenetic location in the tree of life and because it is a hyperthermophilic chemolithoautotroph known as the primary producer of organic matter at deep-sea hydrothermal vents. P. fumarii exhibits currently the highest optimal growth temperature of all life forms on earth (106°C). This is the first completed genome sequence of a member of the genus Pyrolobus to be published and only the second genome sequence from a member of the family Pyrodictiaceae. Although Diversa Corporation announced the completion of sequencing of the P. fumarii genome on September 25, 2001, this sequence was never released to the public. The 1,843,267 bp long genome with its 1,986 protein-coding and 52 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.