The Experts below are selected from a list of 7422 Experts worldwide ranked by ideXlab platform
Jiankun Liu - One of the best experts on this subject based on the ideXlab platform.
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Pyrrolidine Dithiocarbamate enhances hepatic glycogen synthesis and reduces foxo1 mediated gene transcription in type 2 diabetic rats
American Journal of Physiology-endocrinology and Metabolism, 2012Co-Authors: Tienian Zhu, Ruijing Zhao, Lizhong Zhang, Michel Bernier, Jiankun LiuAbstract:The aim of the present study was to examine the effects of Pyrrolidine Dithiocarbamate (PDTC) on hepatic glycogen synthesis and FoxO1 transcriptional activity in type 2 diabetic rats and the mechan...
Tienian Zhu - One of the best experts on this subject based on the ideXlab platform.
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Pyrrolidine Dithiocarbamate enhances hepatic glycogen synthesis and reduces foxo1 mediated gene transcription in type 2 diabetic rats
American Journal of Physiology-endocrinology and Metabolism, 2012Co-Authors: Tienian Zhu, Ruijing Zhao, Lizhong Zhang, Michel Bernier, Jiankun LiuAbstract:The aim of the present study was to examine the effects of Pyrrolidine Dithiocarbamate (PDTC) on hepatic glycogen synthesis and FoxO1 transcriptional activity in type 2 diabetic rats and the mechan...
Alexander M. Seifalian - One of the best experts on this subject based on the ideXlab platform.
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The in-vivo effect of Pyrrolidine Dithiocarbamate on hepatic parenchymal microcirculation and oxygenation of the rat liver.
European journal of gastroenterology & hepatology, 2009Co-Authors: Alexander M. Seifalian, Ih Mallick, Esmaeil Hajinasrollah, Amaki L. Sogbodjor, Bengt Gustafsson, Dick Delbro, Marc C. WinsletAbstract:OBJECTIVE: Pyrrolidine Dithiocarbamate has been shown to be a potent inducer of haemeoxygenase-1. This study investigated its in-vivo effects on systemic and hepatic microcirculatory perfusion. METHODS: Male Sprague-Dawley rats (n=12) were administered intravenously with Pyrrolidine Dithiocarbamate (10, 20 and 50 mg/kg body weight) or vehicle (0.2 ml physiological saline) served as control. Systemic and hepatic haemodynamics including arterial oxygen saturation, heart rate, mean arterial blood pressure and portal blood flow were monitored. Microcirculation in skeletal muscle and liver was measured by laser Doppler flowmetry and intravital fluorescence microscopy, whereas hepatic tissue oxyhaemoglobin and cytochrome oxidase CuA redox state, which is an indicative of extracellular and intracellular oxygenation were measured by near infrared spectroscopy. RESULTS: Pyrrolidine Dithiocarbamate induced a dose-dependent increase in mean arterial blood pressure and skeletal muscle microcirculation. The hepatic parenchymal microcirculation was significantly improved and an increase in sinusoidal diameter and reduction in RBC velocity were observed. Pyrrolidine Dithiocarbamate also showed beneficial effect on hepatic tissue oxygenation showed by an increase in oxyhaemoglobin and cytochrome oxidase CuA redox state as well. CONCLUSION: Pyrrolidine Dithiocarbamate improves hepatic parenchymal microcirculation and tissue oxygenation, suggesting that it may be used as a potential agent in pharmacological preconditioning in the liver.
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Pyrrolidine Dithiocarbamate reduces ischemia-reperfusion injury of the small intestine.
World journal of gastroenterology, 2005Co-Authors: Ih Mallick, Marc C. Winslet, Wenxuan Yang, Alexander M. SeifalianAbstract:AIM: To evaluate whether Pyrrolidine Dithiocarbamate (PDTC), an enhancer of HO production, attenuates intestinal IR injury. METHODS: Eighteen male rats were randomly allocated into three groups: (a) sham; (b) IR, consisting of 30 min of intestinal ischemia, followed by 2-h period of reperfusion; and (c) PDTC treatment before IR. Intestinal microvascular perfusion (IMP) was monitored continuously by laser Doppler fl owmetry. At the end of the reperfusion, serum samples for lactate dehydrogenase (LDH) levels and biopsies of ileum were obtained. HO activity in the ileum was assessed at the end of the reperfusion period. RESULTS: At the end of the reperfusion in the IR group, IMP recovered partially to 42.5% of baseline (P
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Pyrrolidine Dithiocarbamate reduces ischemia reperfusion injury of the small intestine
World Journal of Gastroenterology, 2005Co-Authors: Ih Mallick, Marc C. Winslet, Wenxuan Yang, Alexander M. SeifalianAbstract:AIM: To evaluate whether Pyrrolidine Dithiocarbamate (PDTC), an enhancer of HO production, attenuates intestinal IR injury. METHODS: Eighteen male rats were randomly allocated into three groups: (a) sham; (b) IR, consisting of 30 min of intestinal ischemia, followed by 2-h period of reperfusion; and (c) PDTC treatment before IR. Intestinal microvascular perfusion (IMP) was monitored continuously by laser Doppler fl owmetry. At the end of the reperfusion, serum samples for lactate dehydrogenase (LDH) levels and biopsies of ileum were obtained. HO activity in the ileum was assessed at the end of the reperfusion period. RESULTS: At the end of the reperfusion in the IR group, IMP recovered partially to 42.5% of baseline (P <0.05 vs sham), whereas PDTC improved IMP to 67.3% of baseline (P <0.01 vs IR). There was a twofold increase in HO activity in PDTC group (2 062.66±106.11) as compared to IR (842.3±85.12) (P <0.001). LDH was signifi cantly reduced (P<0.001) in PDTC group (585.6±102.4) as compared to IR group (1 973.8±306.5). Histological examination showed that the ileal mucosa was signifi cantly less injured in PDTC group as compared with IR group. CONCLUSION: Our study demonstrates that PDTC improves the IMP and attenuates IR injury of the intestine possibly via HO production. Additional studies are warranted to evaluate the clinical effi cacy of PDTC in the prevention of IR injury of the small intestine.
Constant M.g. Van Den Berg - One of the best experts on this subject based on the ideXlab platform.
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Determination of arsenic in sea water by cathodic stripping voltammetry in the presence of Pyrrolidine Dithiocarbamate
Analytica Chimica Acta, 1994Co-Authors: Jiri Zima, Constant M.g. Van Den BergAbstract:Abstract A procedure is presented for the determination of arsenite and total arsenic in sea water using differential pulse cathodic stripping voltammetry at a hanging mercury drop electrode in the presence of Pyrrolidine Dithiocarbamate (PDC). The deposition potential was −0.3 V, whereas the scan was initiated from 0 V. Optimised conditions include a PDC concentration of 0.5 μM and a pH ≈ 0.8. The detection limit of the method is 3 nM As. The method was tested by analysis of CASS-2 certified sea water and applied to determination of arsenic in Atlantic ocean water.
Ruijing Zhao - One of the best experts on this subject based on the ideXlab platform.
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Pyrrolidine Dithiocarbamate enhances hepatic glycogen synthesis and reduces foxo1 mediated gene transcription in type 2 diabetic rats
American Journal of Physiology-endocrinology and Metabolism, 2012Co-Authors: Tienian Zhu, Ruijing Zhao, Lizhong Zhang, Michel Bernier, Jiankun LiuAbstract:The aim of the present study was to examine the effects of Pyrrolidine Dithiocarbamate (PDTC) on hepatic glycogen synthesis and FoxO1 transcriptional activity in type 2 diabetic rats and the mechan...
