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Theerapong Krajaejun - One of the best experts on this subject based on the ideXlab platform.
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Draft genome sequence of the oomycete Pythium destruens strain ATCC 64221 from a horse with Pythiosis in Australia
BMC Research Notes, 2020Co-Authors: Theerapong Krajaejun, Tassanee Lohnoo, Preecha Patumcharoenpol, Thidarat Rujirawat, Weerayuth Kittichotirat, Wanta YingyongAbstract:Objectives Genome sequences are a vital resource for accelerating the biological exploration of an organism of interest. Pythium destruens (a synonym of Pythium insidiosum ) causes a difficult-to-treat infectious disease called Pythiosis worldwide. Detection and management of Pythiosis are challenging. Basic knowledge of the disease is lacking. Genomes of this organism isolated from different continents (i.e., Asia and the Americas) have been sequenced and publicly available. Here, we sequenced the genome of an Australian isolate of P. destruens . Genome data will facilitate the comparative analysis of this and related species at the molecular level. Data description Genomic DNA of the P. destruens strain ATCC 64221, isolated from a horse with Pythiosis in Australia, was used to prepare one paired-end library (with 180-bp insert) for next-generation sequencing, using the Illumina HiSeq 2500 short-read platform. Raw reads were cleaned and assembled by several bioinformatics tools. A total of 20,860,454 processed reads, accounted for 2,614,890,553 total bases, can be assembled into a 37.8-Mb genome, consisting 13,060 contigs (average length: 2896 bases; range: 300–142,967), N _50 of 11,370 bases, and 2.9% ‘N’ composition. The genome was determined 85.9% completeness, contained 14,424 predicted genes, and can be retrieved online at the NCBI/DDBJ databases under the accession number BCFQ01000000.1.
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Protein A/G-based enzyme-linked immunosorbent assay for detection of anti-Pythium insidiosum antibodies in human and animal subjects
BMC Research Notes, 2020Co-Authors: Chalisa Jaturapaktrarak, Penpan Payattikul, Tassanee Lohnoo, Yothin Kumsang, Aree Laikul, Watcharapol Pathomsakulwong, Chompoonek Yurayart, Walaiporn Tonpitak, Theerapong KrajaejunAbstract:Objectives Pythiosis is a deadly infectious disease caused by Pythium insidiosum. Reports of both human and animal Pythiosis are on the rise worldwide. Prognosis of the Pythiosis patients relies on early diagnosis and prompt treatment. There are needs for an immunodiagnostic test that can detect the disease in both humans and animals. This study aims at reporting an optimized protocol for the development of a protein A/G-based enzyme-linked immunosorbent assay (ELISA) for the detection of anti- P. insidiosum antibody in multiple host species. Results A total of 25 Pythiosis and 50 control sera, obtained from humans, horses, dogs, cats, and cows, were recruited for the assay development. With a proper ELISA cutoff point, all Pythiosis sera can ultimately be distinguished from the control sera. The successfully-developed protein A/G-based ELISA can detect the anti- P. insidiosum antibodies in serum samples of both humans and animals. It is a versatile, feasible-to-develop, and functional immunodiagnostic assay for Pythiosis.
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Recent update in diagnosis and treatment of human Pythiosis.
PeerJ, 2020Co-Authors: Maria N. Chitasombat, Passara Jongkhajornpong, Kaevalin Lekhanont, Theerapong KrajaejunAbstract:Human Pythiosis is an infectious condition with high morbidity and mortality. The causative agent is the oomycete microorganism Pythium insidiosum. The pathogen inhabits ubiquitously in a wet environment, and direct exposure to the pathogen initiates the infection. Most patients with Pythiosis require surgical removal of the affected organ, and many patients die from the disease. Awareness of Pythiosis among healthcare personnel is increasing. In this review, we summarized and updated information on the diagnosis and treatment of human Pythiosis. Vascular and ocular Pythiosis are common clinical manifestations. Recognition of the typical clinical features of Pythiosis is essential for early diagnosis. The definitive diagnosis of the disease requires laboratory testing, such as microbiological, serological, molecular, and proteomic assays. In vascular Pythiosis, surgical intervention to achieve the organism-free margin of the affected tissue, in combination with the use of antifungal drugs and P. insidiosum immunotherapy, remains the recommended treatment. Ocular Pythiosis is a serious condition and earliest therapeutic penetrating keratoplasty with wide surgical margin is the mainstay treatment. Thorough clinical assessment is essential in all patients to evaluate the treatment response and detect an early sign of the disease recurrence. In conclusion, early diagnosis and proper management are the keys to an optimal outcome of the patients with Pythiosis.
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Data on whole genome sequencing of the oomycete Pythium insidiosum strain CBS 101555 from a horse with Pythiosis in Brazil
BMC Research Notes, 2018Co-Authors: Theerapong Krajaejun, Tassanee Lohnoo, Preecha Patumcharoenpol, Thidarat Rujirawat, Weerayuth Kittichotirat, Wanta YingyongAbstract:Objectives The oomycete Pythium insidiosum infects humans and animals worldwide, and causes the life-threatening condition, called pythosis. Most patients lose infected organs or die from the disease. Comparative genomic analyses of different P. insidiosum strains could provide new insights into its pathobiology, and can lead to discovery of an effective treatment method. Several draft genomes of P. insidiosum are publicly available: three from Asia (Thailand), and one each from North (the United States) and Central (Costa Rica) Americas. We report another draft genome of P. insidiosum isolated from South America (Brazil), to serve as a resource for comprehensive genomic studies. Data description In this study, we report genome sequence of the P. insidiosum strain CBS 101555, isolated from a horse with Pythiosis in Brazil. One paired-end (180-bp insert) library of processed genomic DNA was prepared for Illumina HiSeq 2500-based sequencing. Assembly of raw reads provided genome size of 48.9 Mb, comprising 60,602 contigs. A total of 23,254 genes were predicted and classified into 18,305 homologous gene clusters. Compared with the reference genome (the P. insidiosum strain Pi-S), 1,475,337 sequence variants (SNPs and INDELs) were identified in the organism. The genome sequence data has been deposited in DDBJ under the accession numbers BCFP01000001–BCFP01060602.
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First confirmed case of nasal Pythiosis in a horse in Thailand.
JMM Case Reports, 2018Co-Authors: Walaiporn Tonpitak, Theerapong Krajaejun, Watcharapol Pathomsakulwong, Chulabha Sornklien, Suppathat WutthiwithayaphongAbstract:Introduction. Pythiosis is caused by Pythium insidiosum, a fungus-like organism in the class Oomycetes. It can infect humans and a variety of animal species in tropical, subtropical and some temperate regions. Cases of animal Pythiosis have occurred predominantly in horses in the skin and subcutaneous tissue at the limbs and in the ventral portion of thoracoabdominal wall - lesions in the nasal region are rarely reported. Moreover, although many human Pythiosis cases have been reported in Thailand, no cases of animal Pythiosis in Thailand have been reported. Case presentation. We report a case of Pythiosis in a horse infected at the nasal cavity. Diagnosis was performed by zoospore formation by bait technique, immunohistochemical stain, immunochromatography and sequence analysis. Conclusion. The sequences of rDNA were 99 % and 96 to 99 % identical to GenBank isolates of Pythium insidiosum from two Thai human patients and horses from various countries, respectively. This represents the first confirmed report of nasal equine Pythiosis in Thailand.
Wanta Yingyong - One of the best experts on this subject based on the ideXlab platform.
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Draft genome sequence of the oomycete Pythium destruens strain ATCC 64221 from a horse with Pythiosis in Australia
BMC Research Notes, 2020Co-Authors: Theerapong Krajaejun, Tassanee Lohnoo, Preecha Patumcharoenpol, Thidarat Rujirawat, Weerayuth Kittichotirat, Wanta YingyongAbstract:Objectives Genome sequences are a vital resource for accelerating the biological exploration of an organism of interest. Pythium destruens (a synonym of Pythium insidiosum ) causes a difficult-to-treat infectious disease called Pythiosis worldwide. Detection and management of Pythiosis are challenging. Basic knowledge of the disease is lacking. Genomes of this organism isolated from different continents (i.e., Asia and the Americas) have been sequenced and publicly available. Here, we sequenced the genome of an Australian isolate of P. destruens . Genome data will facilitate the comparative analysis of this and related species at the molecular level. Data description Genomic DNA of the P. destruens strain ATCC 64221, isolated from a horse with Pythiosis in Australia, was used to prepare one paired-end library (with 180-bp insert) for next-generation sequencing, using the Illumina HiSeq 2500 short-read platform. Raw reads were cleaned and assembled by several bioinformatics tools. A total of 20,860,454 processed reads, accounted for 2,614,890,553 total bases, can be assembled into a 37.8-Mb genome, consisting 13,060 contigs (average length: 2896 bases; range: 300–142,967), N _50 of 11,370 bases, and 2.9% ‘N’ composition. The genome was determined 85.9% completeness, contained 14,424 predicted genes, and can be retrieved online at the NCBI/DDBJ databases under the accession number BCFQ01000000.1.
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Data on whole genome sequencing of the oomycete Pythium insidiosum strain CBS 101555 from a horse with Pythiosis in Brazil
BMC Research Notes, 2018Co-Authors: Theerapong Krajaejun, Tassanee Lohnoo, Preecha Patumcharoenpol, Thidarat Rujirawat, Weerayuth Kittichotirat, Wanta YingyongAbstract:Objectives The oomycete Pythium insidiosum infects humans and animals worldwide, and causes the life-threatening condition, called pythosis. Most patients lose infected organs or die from the disease. Comparative genomic analyses of different P. insidiosum strains could provide new insights into its pathobiology, and can lead to discovery of an effective treatment method. Several draft genomes of P. insidiosum are publicly available: three from Asia (Thailand), and one each from North (the United States) and Central (Costa Rica) Americas. We report another draft genome of P. insidiosum isolated from South America (Brazil), to serve as a resource for comprehensive genomic studies. Data description In this study, we report genome sequence of the P. insidiosum strain CBS 101555, isolated from a horse with Pythiosis in Brazil. One paired-end (180-bp insert) library of processed genomic DNA was prepared for Illumina HiSeq 2500-based sequencing. Assembly of raw reads provided genome size of 48.9 Mb, comprising 60,602 contigs. A total of 23,254 genes were predicted and classified into 18,305 homologous gene clusters. Compared with the reference genome (the P. insidiosum strain Pi-S), 1,475,337 sequence variants (SNPs and INDELs) were identified in the organism. The genome sequence data has been deposited in DDBJ under the accession numbers BCFP01000001–BCFP01060602.
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Draft genome sequences of the oomycete Pythium insidiosum strain CBS 573.85 from a horse with Pythiosis and strain CR02 from the environment
Elsevier, 2018Co-Authors: Preecha Patumcharoenpol, Nongnuch Vanittanakom, Tassanee Lohnoo, Wanta Yingyong, Thidarat Rujirawat, Weerayuth Kittichotirat, Theerapong KrajaejunAbstract:Pythium insidiosum is an aquatic oomycete microorganism that causes the fatal infectious disease, Pythiosis, in humans and animals. The organism has been successfully isolated from the environment worldwide. Diagnosis and treatment of Pythiosis is difficult and challenging. Genome sequences of P. insidiosum, isolated from humans, are available and accessible in public databases. To further facilitate biology-, pathogenicity-, and evolution-related genomic and genetic studies of P. insidiosum, we report two additional draft genome sequences of the P. insidiosum strain CBS 573.85 (35.6 Mb in size; accession number, BCFO00000000.1) isolated from a horse with Pythiosis, and strain CR02 (37.7 Mb in size; accession number, BCFR00000000.1) isolated from the environment. Keywords: Pythium insidiosum, Pythiosis, Draft genome sequenc
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Draft genome sequences of the oomycete Pythium insidiosum strain CBS 573.85 from a horse with Pythiosis and strain CR02 from the environment
Data in Brief, 2018Co-Authors: Preecha Patumcharoenpol, Nongnuch Vanittanakom, Tassanee Lohnoo, Wanta Yingyong, Thidarat Rujirawat, Weerayuth Kittichotirat, Theerapong KrajaejunAbstract:Pythium insidiosum is an aquatic oomycete microorganism that causes the fatal infectious disease, Pythiosis, in humans and animals. The organism has been successfully isolated from the environment worldwide. Diagnosis and treatment of Pythiosis is difficult and challenging. Genome sequences of P. insidiosum, isolated from humans, are available and accessible in public databases. To further facilitate biology-, pathogenicity-, and evolution-related genomic and genetic studies of P. insidiosum, we report two additional draft genome sequences of the P. insidiosum strain CBS 573.85 (35.6 Mb in size; accession number, BCFO00000000.1) isolated from a horse with Pythiosis, and strain CR02 (37.7 Mb in size; accession number, BCFR00000000.1) isolated from the environment.
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Assessment of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification and biotyping of the pathogenic oomycete Pythium insidiosum
Elsevier, 2018Co-Authors: Theerapong Krajaejun, Tassanee Lohnoo, Yothin Kumsang, Wanta Yingyong, Thidarat Rujirawat, Paisan Jittorntam, Atthapol Srimongkol, Onrapak Reamtong, Suthee MangmeeAbstract:Objective: Pythiosis is a life-threatening infectious disease caused by the oomycete Pythium insidiosum. The disease has been increasingly reported worldwide. Most patients with Pythiosis undergo surgical removal of an infected organ. Early diagnosis contributes to better prognosis of Pythiosis patients. Here, we assessed the matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for identification and biotyping of P. insidiosum. Methods: A comprehensive set of mass spectra of P. insidiosum was generated to evaluate MALDI-TOF MS for identification and biotyping of P. insidiosum. Results: MALDI-TOF MS accurately identified all 13 P. insidiosum strains tested, at the species level. Mass spectra of P. insidiosum did not match any other microorganisms, including fungi (i.e., Aspergillus species, Fusarium species, and fungal species of the class Zygomycetes), which have similar microscopic morphologies with this oomycete. MALDI-TOF MS- and rDNA sequence-based biotyping methods consistently classified P. insidiosum into three groups: Clade-I (American strains), II (Asian and Australian strains), and III (mostly Thai strains). Conclusions: MALDI-TOF MS has been successfully used for identification and biotyping of P. insidiosum. The obtained mass spectral database allows clinical microbiology laboratories, well-equipped with a MALDI-TOF mass spectrometer, to conveniently identify P. insidiosum, without requiring any pathogen-specific reagents (i.e., antigen, antibody or primers). Keywords: Pythium insidiosum, Pythiosis, MALDI-TOF MS, Mass spectrum, Identification, Biotypin
Tassanee Lohnoo - One of the best experts on this subject based on the ideXlab platform.
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Draft genome sequence of the oomycete Pythium destruens strain ATCC 64221 from a horse with Pythiosis in Australia
BMC Research Notes, 2020Co-Authors: Theerapong Krajaejun, Tassanee Lohnoo, Preecha Patumcharoenpol, Thidarat Rujirawat, Weerayuth Kittichotirat, Wanta YingyongAbstract:Objectives Genome sequences are a vital resource for accelerating the biological exploration of an organism of interest. Pythium destruens (a synonym of Pythium insidiosum ) causes a difficult-to-treat infectious disease called Pythiosis worldwide. Detection and management of Pythiosis are challenging. Basic knowledge of the disease is lacking. Genomes of this organism isolated from different continents (i.e., Asia and the Americas) have been sequenced and publicly available. Here, we sequenced the genome of an Australian isolate of P. destruens . Genome data will facilitate the comparative analysis of this and related species at the molecular level. Data description Genomic DNA of the P. destruens strain ATCC 64221, isolated from a horse with Pythiosis in Australia, was used to prepare one paired-end library (with 180-bp insert) for next-generation sequencing, using the Illumina HiSeq 2500 short-read platform. Raw reads were cleaned and assembled by several bioinformatics tools. A total of 20,860,454 processed reads, accounted for 2,614,890,553 total bases, can be assembled into a 37.8-Mb genome, consisting 13,060 contigs (average length: 2896 bases; range: 300–142,967), N _50 of 11,370 bases, and 2.9% ‘N’ composition. The genome was determined 85.9% completeness, contained 14,424 predicted genes, and can be retrieved online at the NCBI/DDBJ databases under the accession number BCFQ01000000.1.
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Protein A/G-based enzyme-linked immunosorbent assay for detection of anti-Pythium insidiosum antibodies in human and animal subjects
BMC Research Notes, 2020Co-Authors: Chalisa Jaturapaktrarak, Penpan Payattikul, Tassanee Lohnoo, Yothin Kumsang, Aree Laikul, Watcharapol Pathomsakulwong, Chompoonek Yurayart, Walaiporn Tonpitak, Theerapong KrajaejunAbstract:Objectives Pythiosis is a deadly infectious disease caused by Pythium insidiosum. Reports of both human and animal Pythiosis are on the rise worldwide. Prognosis of the Pythiosis patients relies on early diagnosis and prompt treatment. There are needs for an immunodiagnostic test that can detect the disease in both humans and animals. This study aims at reporting an optimized protocol for the development of a protein A/G-based enzyme-linked immunosorbent assay (ELISA) for the detection of anti- P. insidiosum antibody in multiple host species. Results A total of 25 Pythiosis and 50 control sera, obtained from humans, horses, dogs, cats, and cows, were recruited for the assay development. With a proper ELISA cutoff point, all Pythiosis sera can ultimately be distinguished from the control sera. The successfully-developed protein A/G-based ELISA can detect the anti- P. insidiosum antibodies in serum samples of both humans and animals. It is a versatile, feasible-to-develop, and functional immunodiagnostic assay for Pythiosis.
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Data on whole genome sequencing of the oomycete Pythium insidiosum strain CBS 101555 from a horse with Pythiosis in Brazil
BMC Research Notes, 2018Co-Authors: Theerapong Krajaejun, Tassanee Lohnoo, Preecha Patumcharoenpol, Thidarat Rujirawat, Weerayuth Kittichotirat, Wanta YingyongAbstract:Objectives The oomycete Pythium insidiosum infects humans and animals worldwide, and causes the life-threatening condition, called pythosis. Most patients lose infected organs or die from the disease. Comparative genomic analyses of different P. insidiosum strains could provide new insights into its pathobiology, and can lead to discovery of an effective treatment method. Several draft genomes of P. insidiosum are publicly available: three from Asia (Thailand), and one each from North (the United States) and Central (Costa Rica) Americas. We report another draft genome of P. insidiosum isolated from South America (Brazil), to serve as a resource for comprehensive genomic studies. Data description In this study, we report genome sequence of the P. insidiosum strain CBS 101555, isolated from a horse with Pythiosis in Brazil. One paired-end (180-bp insert) library of processed genomic DNA was prepared for Illumina HiSeq 2500-based sequencing. Assembly of raw reads provided genome size of 48.9 Mb, comprising 60,602 contigs. A total of 23,254 genes were predicted and classified into 18,305 homologous gene clusters. Compared with the reference genome (the P. insidiosum strain Pi-S), 1,475,337 sequence variants (SNPs and INDELs) were identified in the organism. The genome sequence data has been deposited in DDBJ under the accession numbers BCFP01000001–BCFP01060602.
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Draft genome sequences of the oomycete Pythium insidiosum strain CBS 573.85 from a horse with Pythiosis and strain CR02 from the environment
Elsevier, 2018Co-Authors: Preecha Patumcharoenpol, Nongnuch Vanittanakom, Tassanee Lohnoo, Wanta Yingyong, Thidarat Rujirawat, Weerayuth Kittichotirat, Theerapong KrajaejunAbstract:Pythium insidiosum is an aquatic oomycete microorganism that causes the fatal infectious disease, Pythiosis, in humans and animals. The organism has been successfully isolated from the environment worldwide. Diagnosis and treatment of Pythiosis is difficult and challenging. Genome sequences of P. insidiosum, isolated from humans, are available and accessible in public databases. To further facilitate biology-, pathogenicity-, and evolution-related genomic and genetic studies of P. insidiosum, we report two additional draft genome sequences of the P. insidiosum strain CBS 573.85 (35.6 Mb in size; accession number, BCFO00000000.1) isolated from a horse with Pythiosis, and strain CR02 (37.7 Mb in size; accession number, BCFR00000000.1) isolated from the environment. Keywords: Pythium insidiosum, Pythiosis, Draft genome sequenc
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Draft genome sequences of the oomycete Pythium insidiosum strain CBS 573.85 from a horse with Pythiosis and strain CR02 from the environment
Data in Brief, 2018Co-Authors: Preecha Patumcharoenpol, Nongnuch Vanittanakom, Tassanee Lohnoo, Wanta Yingyong, Thidarat Rujirawat, Weerayuth Kittichotirat, Theerapong KrajaejunAbstract:Pythium insidiosum is an aquatic oomycete microorganism that causes the fatal infectious disease, Pythiosis, in humans and animals. The organism has been successfully isolated from the environment worldwide. Diagnosis and treatment of Pythiosis is difficult and challenging. Genome sequences of P. insidiosum, isolated from humans, are available and accessible in public databases. To further facilitate biology-, pathogenicity-, and evolution-related genomic and genetic studies of P. insidiosum, we report two additional draft genome sequences of the P. insidiosum strain CBS 573.85 (35.6 Mb in size; accession number, BCFO00000000.1) isolated from a horse with Pythiosis, and strain CR02 (37.7 Mb in size; accession number, BCFR00000000.1) isolated from the environment.
Ariya Chindamporn - One of the best experts on this subject based on the ideXlab platform.
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A case of canine cutaneous Pythiosis in Thailand.
Access Microbiology, 2020Co-Authors: Ariya Chindamporn, Patcharee Kammarnjessadakul, Sawang Kesdangsakonwut, Wijit BanlunaraAbstract:Introduction Pythium insidiosum causes Pythiosis in humans and animals in tropical and subtropical climates. The clinical manifestations in humans are mostly systemic, vascular or ocular forms, in contrast to animals, which are cutaneous, subcutaneous and gastrointestinal forms. The highest incidence of human cases is reported in Thailand, however, no canine Pythiosis has been documented yet. Case presentation A female, mixed-breed, stray dog showed severe extensive ulcerative haemorrhagic dermatitis at the perineum involving the anus and tail. On cytology, there were sparse branching septate fungal hyphae. The tissue samples were subjected to polymerase chain reaction and gene sequencing for fungal identification. Conclusion The results of the internal transcribed spacer 1 and 2 (ITS1 and ITS2) gene had 99 % homology to Pythium insidiosum (accession no. FJ17396) and the COX2 gene (accession no. GQ451572). The phylogenetic tree of both genes was classified in clade ATH. This is the first fully documented diagnosis of canine cutaneous Pythiosis in Thailand.
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Human Pythiosis: Emergence of Fungal-Like Organism
Mycopathologia, 2019Co-Authors: Nitipong Permpalung, Navaporn Worasilchai, Ariya ChindampornAbstract:Pythiosis is an emerging infectious disease caused by the aquatic oomycete Pythium insidiosum , a fungal-like organism. It is believed that P. insidiosum ’s zoospores, its infected form, play major role in pathogenesis. Vascular and ocular infections are the most common clinical manifestation in humans. It is difficult to establish the diagnosis given its relatively rarity and difficulty to distinguish P. insidiosum from other molds. Delay in diagnosis and treatment has been associated with poor outcomes. High index of suspicion is the key, particularly in thalassemia patients with arterial insufficiency and patients with fungal keratitis/endophthalmitis without improvement on antifungal therapy. Tissue culture and zoospore induction remain gold standard for diagnosis; however, DNA-based method should be performed simultaneously. The combination of radical surgery, antifungal agents, and immunotherapy has been recommended. It was previously believed that surgery with negative surgical margins was the essential to survive in vascular Pythiosis; however, it was recently found that patients could have residual disease despite documented negative surgical margins as infected clot may be dislodged to proximal arterial sites prior to surgery. Serum β- d -glucan (BG) has been used to monitor disease response after treatment initiation in vascular Pythiosis. A significant decrease in BG levels within 2 weeks after surgery is indicative of the absence of residual infection. Unfortunately, monitoring tools for ocular Pythiosis are not yet available. Itraconazole plus terbinafine have generally been used in P. insidiosum -infected patients; however, antibacterial agents, including azithromycin and linezolid, have also been used with favorable outcomes in ocular disease. Recently, azithromycin or clarithromycin plus doxycyclin were used in two relapsed vascular Pythiosis patients with good outcomes.
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Clinical outcomes in ocular Pythiosis patients treated with a combination therapy protocol in Thailand: A prospective study.
Medical Mycology, 2019Co-Authors: Nitipong Permpalung, Navaporn Worasilchai, Kasama Manothummetha, Pattama Torvorapanit, Kitiya Ratanawongphaibul, Nipat Chuleerarux, Rongpong Plongla, Ariya ChindampornAbstract:Ocular Pythiosis is the second most common form of human Pythiosis, and the rates of evisceration/enucleation in Thailand are 55-79%. This prospective study was conducted to evaluate treatment outcomes of the combination therapy protocol and the potential use of serum (1→3)-β-glucan (BG) and Pythium insidiosum-specific antibody (Pi-Ab) as an aid to diagnosis and monitoring of ocular Pythiosis. Thirty patients were enrolled in the study and 14 (non-globe salvage) required evisceration/enucleation. The globe salvage group was significantly younger, and first ocular surgeries were performed significantly sooner than in the non-globe salvage group. Serum BG and Pi-Ab levels were similar among the 2 groups over time. In vitro susceptibility testing of antifungal agents revealed relatively high minimum inhibitory concentrations and lack of synergistic effect. Serum BG and Pi-Ab would not be useful in diagnosis and monitoring of ocular Pythiosis. Until effective antimicrobial agents are discovered, ocular surgeries are still the mainstay therapy in Thailand.
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Adjunctive antibacterial agents as a salvage therapy in relapsed vascular Pythiosis patients.
International Journal of Infectious Diseases, 2019Co-Authors: Nuttapon Susaengrat, Ariya Chindamporn, Navaporn Worasilchai, Kasama Manothummetha, Pattama Torvorapanit, Nipat Chuleerarux, Rongpong Plongla, Jarruprot Tuangsirisup, Nitipong PermpalungAbstract:Abstract Human vascular Pythiosis is a life-threatening condition caused by Pythium insidiosum. Patients with unresectable intra-abdominal artery involvement have not previously survived, despite being treated with antifungal agents and immunotherapy. We report two novel cases of intra-abdominal Pythiosis in patients for whom surgery could not be performed, who were successfully treated with adjunctive antibacterial agents.
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monitoring anti pythium insidiosum igg antibodies and 1 3 β d glucan in vascular Pythiosis
bioRxiv, 2018Co-Authors: Navaporn Worasilchai, Leonel Mendoza, Nitipong Permpalung, Pakawat Chongsathidkiet, Asada Leelahavanichkul, Tanapat Palaga, Rangsima Reantragoon, Malcolm Finkelman, Pranee Sutcharitchan, Ariya ChindampornAbstract:Background: Despite aggressive treatment, vascular Pythiosis has a mortality rate of 40%. This is due to the delay in diagnosis and lack of effective monitoring tools. To overcome this drawback, serum beta-D-glucan (BG) and P. insidiosum specific antibody ( Pi -Ab) were examined as potential monitoring markers in vascular Pythiosis. Methods: A prospective cohort study of vascular Pythiosis patients was carried on during January 2010-July 2016. Clinical information and blood samples were collected and evaluated by the BG and Pi -Ab assays. Linear mixed effect models were used to compare BG and Pi -Ab levels. The in vitro susceptibility test was performed in all P. insidiosum isolates from culture positive cases. Results: A total of 50 patients were enrolled; 45 survived and 5 died during follow-up. The survivors had a significantly shorter time to medical care (p Pi -Ab above 8, whereas EV among deceased patients was less than 4. In vitro susceptibility results revealed no synergistic effects between itraconazole and terbinafine. Conclusions: This study showed that BG and Pi -Ab were potentially valuable markers to monitor the disease after treatment initiation. An unchanged BG level at 2 weeks after surgery should prompt an evaluation for residual disease.
Preecha Patumcharoenpol - One of the best experts on this subject based on the ideXlab platform.
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Draft genome sequence of the oomycete Pythium destruens strain ATCC 64221 from a horse with Pythiosis in Australia
BMC Research Notes, 2020Co-Authors: Theerapong Krajaejun, Tassanee Lohnoo, Preecha Patumcharoenpol, Thidarat Rujirawat, Weerayuth Kittichotirat, Wanta YingyongAbstract:Objectives Genome sequences are a vital resource for accelerating the biological exploration of an organism of interest. Pythium destruens (a synonym of Pythium insidiosum ) causes a difficult-to-treat infectious disease called Pythiosis worldwide. Detection and management of Pythiosis are challenging. Basic knowledge of the disease is lacking. Genomes of this organism isolated from different continents (i.e., Asia and the Americas) have been sequenced and publicly available. Here, we sequenced the genome of an Australian isolate of P. destruens . Genome data will facilitate the comparative analysis of this and related species at the molecular level. Data description Genomic DNA of the P. destruens strain ATCC 64221, isolated from a horse with Pythiosis in Australia, was used to prepare one paired-end library (with 180-bp insert) for next-generation sequencing, using the Illumina HiSeq 2500 short-read platform. Raw reads were cleaned and assembled by several bioinformatics tools. A total of 20,860,454 processed reads, accounted for 2,614,890,553 total bases, can be assembled into a 37.8-Mb genome, consisting 13,060 contigs (average length: 2896 bases; range: 300–142,967), N _50 of 11,370 bases, and 2.9% ‘N’ composition. The genome was determined 85.9% completeness, contained 14,424 predicted genes, and can be retrieved online at the NCBI/DDBJ databases under the accession number BCFQ01000000.1.
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Data on whole genome sequencing of the oomycete Pythium insidiosum strain CBS 101555 from a horse with Pythiosis in Brazil
BMC Research Notes, 2018Co-Authors: Theerapong Krajaejun, Tassanee Lohnoo, Preecha Patumcharoenpol, Thidarat Rujirawat, Weerayuth Kittichotirat, Wanta YingyongAbstract:Objectives The oomycete Pythium insidiosum infects humans and animals worldwide, and causes the life-threatening condition, called pythosis. Most patients lose infected organs or die from the disease. Comparative genomic analyses of different P. insidiosum strains could provide new insights into its pathobiology, and can lead to discovery of an effective treatment method. Several draft genomes of P. insidiosum are publicly available: three from Asia (Thailand), and one each from North (the United States) and Central (Costa Rica) Americas. We report another draft genome of P. insidiosum isolated from South America (Brazil), to serve as a resource for comprehensive genomic studies. Data description In this study, we report genome sequence of the P. insidiosum strain CBS 101555, isolated from a horse with Pythiosis in Brazil. One paired-end (180-bp insert) library of processed genomic DNA was prepared for Illumina HiSeq 2500-based sequencing. Assembly of raw reads provided genome size of 48.9 Mb, comprising 60,602 contigs. A total of 23,254 genes were predicted and classified into 18,305 homologous gene clusters. Compared with the reference genome (the P. insidiosum strain Pi-S), 1,475,337 sequence variants (SNPs and INDELs) were identified in the organism. The genome sequence data has been deposited in DDBJ under the accession numbers BCFP01000001–BCFP01060602.
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Draft genome sequences of the oomycete Pythium insidiosum strain CBS 573.85 from a horse with Pythiosis and strain CR02 from the environment
Elsevier, 2018Co-Authors: Preecha Patumcharoenpol, Nongnuch Vanittanakom, Tassanee Lohnoo, Wanta Yingyong, Thidarat Rujirawat, Weerayuth Kittichotirat, Theerapong KrajaejunAbstract:Pythium insidiosum is an aquatic oomycete microorganism that causes the fatal infectious disease, Pythiosis, in humans and animals. The organism has been successfully isolated from the environment worldwide. Diagnosis and treatment of Pythiosis is difficult and challenging. Genome sequences of P. insidiosum, isolated from humans, are available and accessible in public databases. To further facilitate biology-, pathogenicity-, and evolution-related genomic and genetic studies of P. insidiosum, we report two additional draft genome sequences of the P. insidiosum strain CBS 573.85 (35.6 Mb in size; accession number, BCFO00000000.1) isolated from a horse with Pythiosis, and strain CR02 (37.7 Mb in size; accession number, BCFR00000000.1) isolated from the environment. Keywords: Pythium insidiosum, Pythiosis, Draft genome sequenc
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Draft genome sequences of the oomycete Pythium insidiosum strain CBS 573.85 from a horse with Pythiosis and strain CR02 from the environment
Data in Brief, 2018Co-Authors: Preecha Patumcharoenpol, Nongnuch Vanittanakom, Tassanee Lohnoo, Wanta Yingyong, Thidarat Rujirawat, Weerayuth Kittichotirat, Theerapong KrajaejunAbstract:Pythium insidiosum is an aquatic oomycete microorganism that causes the fatal infectious disease, Pythiosis, in humans and animals. The organism has been successfully isolated from the environment worldwide. Diagnosis and treatment of Pythiosis is difficult and challenging. Genome sequences of P. insidiosum, isolated from humans, are available and accessible in public databases. To further facilitate biology-, pathogenicity-, and evolution-related genomic and genetic studies of P. insidiosum, we report two additional draft genome sequences of the P. insidiosum strain CBS 573.85 (35.6 Mb in size; accession number, BCFO00000000.1) isolated from a horse with Pythiosis, and strain CR02 (37.7 Mb in size; accession number, BCFR00000000.1) isolated from the environment.
