The Experts below are selected from a list of 1116 Experts worldwide ranked by ideXlab platform
Sun Chul Kang - One of the best experts on this subject based on the ideXlab platform.
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novel Quercetin Derivative tef induces er stress and mitochondria mediated apoptosis in human colon cancer hct 116 cells
Biomedicine & Pharmacotherapy, 2016Co-Authors: Imran Khan, Rekha Jakhar, Souren Paul, Jaehong Han, Monika Bhardwaj, Sun Chul KangAbstract:Abstract Although Quercetin is very well known for its anticancer activity, however it shows some drawbacks. Herein, we have evaluated the apoptotic effect TEF (5, 3′-dihydroxy-3, 7, 4′-triethoxyflavone), a newly synthesized Quercetin Derivative on HCT-116 colon cancer cells. After 24 h of treatment, the proliferation of colon cancer cells was inhibited by TEF. TEF induced apoptosis, as confirmed by the presence of fragmented nuclei, reduced mitochondrial membrane potential, and elevated cytoplasmic and mitochondrial reactive oxygen species (ROS) levels. TEF treatment causes elevation of IRE1-α and activates calcium ions (Ca 2+ ) with concomitant increase in JNK levels. Elevated Ca 2+ ion translocates from ER to mitochondria which leads to ROS release and oxidative stress. TEF treatment further elevated levels of pro-apoptotic factors and down-regulated the level of Bcl2. TEF led to activation of mito-JNK (mitochondrial JNK), which plays a crucial role in activation of oxidative stress and caspase mediated apoptotic cell death. Moreover, JNK inhibition shown to suppress TEF induced apoptosis in HCT-116 colon cancer cells. Therefore, this study reveals the apoptotic role of TEF against HCT-116 cell line via IRE1-α and mito-JNK pathway.
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3 5 7 3 4 pentamethoxyflavone a Quercetin Derivative protects dna from oxidative challenges potential mechanism of action
Journal of Photochemistry and Photobiology B-biology, 2014Co-Authors: Rekha Jakhar, Souren Paul, Young Rong Park, Jaehong Han, Sun Chul KangAbstract:DNA protection is one of the most important strategies in cancer therapy. Since Quercetin and its Derivatives are found to be potent antioxidant agents, they are able to scavenge radicals significantly. Therefore, we focused on the DNA protection activity of 3,5,7,3′,4′-pentamethoxyflavone (PMF), a Quercetin Derivative isolated from Kaemperia parviflora. Although, PMF was found to be a very poor antioxidant compound, still it could remarkably protect DNA from oxidative damage. DNA binding assay showed that PMF bound to the minor groove of DNA, which suggests a possible mechanism for its DNA protective effects. Cellular toxicity assay on RAW 264.7 macrophages showed this compound is very safe for therapeutic applications.
Dolors Verdaguer - One of the best experts on this subject based on the ideXlab platform.
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interactive effects of uv radiation and reduced precipitation on the seasonal leaf phenolic content composition and the antioxidant activity of naturally growing arbutus unedo plants
Journal of Photochemistry and Photobiology B-biology, 2015Co-Authors: Nikolaos Nenadis, Laura Llorens, Agathi Koufogianni, Laura Diaz, Joan Nogue I Font, Josep Abel Gonzalez, Dolors VerdaguerAbstract:The effects of UV radiation and rainfall reduction on the seasonal leaf phenolic content/composition and antioxidant activity of the Mediterranean shrub Arbutus unedo were studied. Naturally growing plants of A. unedo were submitted to 97% UV-B reduction (UVA), 95% UV-A+UV-B reduction (UV0) or near-ambient UV levels (UVBA) under two precipitation regimes (natural rainfall or 10-30% rainfall reduction). Total phenol, flavonol and flavanol contents, levels of eight phenols and antioxidant activity [DPPH(●) radical scavenging and Cu (II) reducing capacity] were measured in sun-exposed leaves at the end of four consecutive seasons. Results showed a significant seasonal variation in the leaf content of phenols of A. unedo, with the lowest values found in spring and the highest in autumn and/or winter. Leaf ontogenetic development and/or a possible effect of low temperatures in autumn/winter may account for such findings. Regardless of the watering regime and the sampling date, plant exposure to UV-B radiation decreased the total flavanol content of leaves, while it increased the leaf content in quercitrin (the most abundant Quercetin Derivative identified). By contrast, UV-A radiation increased the leaf content of theogallin, a gallic acid Derivative. Other phenolic compounds (two Quercetin Derivatives, one of them being avicularin, and one kaempferol Derivative, juglanin), as well as the antioxidant activity of the leaves, showed different responses to UV radiation depending on the precipitation regime. Surprisingly, reduced rainfall significantly decreased the total amount of quantified Quercetin Derivatives as well as the DPPH scavenging activity in A. unedo leaves. To conclude, present findings indicate that leaves of A. unedo can be a good source of antioxidants throughout the year, but especially in autumn and winter.
Rekha Jakhar - One of the best experts on this subject based on the ideXlab platform.
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novel Quercetin Derivative tef induces er stress and mitochondria mediated apoptosis in human colon cancer hct 116 cells
Biomedicine & Pharmacotherapy, 2016Co-Authors: Imran Khan, Rekha Jakhar, Souren Paul, Jaehong Han, Monika Bhardwaj, Sun Chul KangAbstract:Abstract Although Quercetin is very well known for its anticancer activity, however it shows some drawbacks. Herein, we have evaluated the apoptotic effect TEF (5, 3′-dihydroxy-3, 7, 4′-triethoxyflavone), a newly synthesized Quercetin Derivative on HCT-116 colon cancer cells. After 24 h of treatment, the proliferation of colon cancer cells was inhibited by TEF. TEF induced apoptosis, as confirmed by the presence of fragmented nuclei, reduced mitochondrial membrane potential, and elevated cytoplasmic and mitochondrial reactive oxygen species (ROS) levels. TEF treatment causes elevation of IRE1-α and activates calcium ions (Ca 2+ ) with concomitant increase in JNK levels. Elevated Ca 2+ ion translocates from ER to mitochondria which leads to ROS release and oxidative stress. TEF treatment further elevated levels of pro-apoptotic factors and down-regulated the level of Bcl2. TEF led to activation of mito-JNK (mitochondrial JNK), which plays a crucial role in activation of oxidative stress and caspase mediated apoptotic cell death. Moreover, JNK inhibition shown to suppress TEF induced apoptosis in HCT-116 colon cancer cells. Therefore, this study reveals the apoptotic role of TEF against HCT-116 cell line via IRE1-α and mito-JNK pathway.
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3 5 7 3 4 pentamethoxyflavone a Quercetin Derivative protects dna from oxidative challenges potential mechanism of action
Journal of Photochemistry and Photobiology B-biology, 2014Co-Authors: Rekha Jakhar, Souren Paul, Young Rong Park, Jaehong Han, Sun Chul KangAbstract:DNA protection is one of the most important strategies in cancer therapy. Since Quercetin and its Derivatives are found to be potent antioxidant agents, they are able to scavenge radicals significantly. Therefore, we focused on the DNA protection activity of 3,5,7,3′,4′-pentamethoxyflavone (PMF), a Quercetin Derivative isolated from Kaemperia parviflora. Although, PMF was found to be a very poor antioxidant compound, still it could remarkably protect DNA from oxidative damage. DNA binding assay showed that PMF bound to the minor groove of DNA, which suggests a possible mechanism for its DNA protective effects. Cellular toxicity assay on RAW 264.7 macrophages showed this compound is very safe for therapeutic applications.
Souren Paul - One of the best experts on this subject based on the ideXlab platform.
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novel Quercetin Derivative tef induces er stress and mitochondria mediated apoptosis in human colon cancer hct 116 cells
Biomedicine & Pharmacotherapy, 2016Co-Authors: Imran Khan, Rekha Jakhar, Souren Paul, Jaehong Han, Monika Bhardwaj, Sun Chul KangAbstract:Abstract Although Quercetin is very well known for its anticancer activity, however it shows some drawbacks. Herein, we have evaluated the apoptotic effect TEF (5, 3′-dihydroxy-3, 7, 4′-triethoxyflavone), a newly synthesized Quercetin Derivative on HCT-116 colon cancer cells. After 24 h of treatment, the proliferation of colon cancer cells was inhibited by TEF. TEF induced apoptosis, as confirmed by the presence of fragmented nuclei, reduced mitochondrial membrane potential, and elevated cytoplasmic and mitochondrial reactive oxygen species (ROS) levels. TEF treatment causes elevation of IRE1-α and activates calcium ions (Ca 2+ ) with concomitant increase in JNK levels. Elevated Ca 2+ ion translocates from ER to mitochondria which leads to ROS release and oxidative stress. TEF treatment further elevated levels of pro-apoptotic factors and down-regulated the level of Bcl2. TEF led to activation of mito-JNK (mitochondrial JNK), which plays a crucial role in activation of oxidative stress and caspase mediated apoptotic cell death. Moreover, JNK inhibition shown to suppress TEF induced apoptosis in HCT-116 colon cancer cells. Therefore, this study reveals the apoptotic role of TEF against HCT-116 cell line via IRE1-α and mito-JNK pathway.
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3 5 7 3 4 pentamethoxyflavone a Quercetin Derivative protects dna from oxidative challenges potential mechanism of action
Journal of Photochemistry and Photobiology B-biology, 2014Co-Authors: Rekha Jakhar, Souren Paul, Young Rong Park, Jaehong Han, Sun Chul KangAbstract:DNA protection is one of the most important strategies in cancer therapy. Since Quercetin and its Derivatives are found to be potent antioxidant agents, they are able to scavenge radicals significantly. Therefore, we focused on the DNA protection activity of 3,5,7,3′,4′-pentamethoxyflavone (PMF), a Quercetin Derivative isolated from Kaemperia parviflora. Although, PMF was found to be a very poor antioxidant compound, still it could remarkably protect DNA from oxidative damage. DNA binding assay showed that PMF bound to the minor groove of DNA, which suggests a possible mechanism for its DNA protective effects. Cellular toxicity assay on RAW 264.7 macrophages showed this compound is very safe for therapeutic applications.
Jaehong Han - One of the best experts on this subject based on the ideXlab platform.
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novel Quercetin Derivative tef induces er stress and mitochondria mediated apoptosis in human colon cancer hct 116 cells
Biomedicine & Pharmacotherapy, 2016Co-Authors: Imran Khan, Rekha Jakhar, Souren Paul, Jaehong Han, Monika Bhardwaj, Sun Chul KangAbstract:Abstract Although Quercetin is very well known for its anticancer activity, however it shows some drawbacks. Herein, we have evaluated the apoptotic effect TEF (5, 3′-dihydroxy-3, 7, 4′-triethoxyflavone), a newly synthesized Quercetin Derivative on HCT-116 colon cancer cells. After 24 h of treatment, the proliferation of colon cancer cells was inhibited by TEF. TEF induced apoptosis, as confirmed by the presence of fragmented nuclei, reduced mitochondrial membrane potential, and elevated cytoplasmic and mitochondrial reactive oxygen species (ROS) levels. TEF treatment causes elevation of IRE1-α and activates calcium ions (Ca 2+ ) with concomitant increase in JNK levels. Elevated Ca 2+ ion translocates from ER to mitochondria which leads to ROS release and oxidative stress. TEF treatment further elevated levels of pro-apoptotic factors and down-regulated the level of Bcl2. TEF led to activation of mito-JNK (mitochondrial JNK), which plays a crucial role in activation of oxidative stress and caspase mediated apoptotic cell death. Moreover, JNK inhibition shown to suppress TEF induced apoptosis in HCT-116 colon cancer cells. Therefore, this study reveals the apoptotic role of TEF against HCT-116 cell line via IRE1-α and mito-JNK pathway.
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3 5 7 3 4 pentamethoxyflavone a Quercetin Derivative protects dna from oxidative challenges potential mechanism of action
Journal of Photochemistry and Photobiology B-biology, 2014Co-Authors: Rekha Jakhar, Souren Paul, Young Rong Park, Jaehong Han, Sun Chul KangAbstract:DNA protection is one of the most important strategies in cancer therapy. Since Quercetin and its Derivatives are found to be potent antioxidant agents, they are able to scavenge radicals significantly. Therefore, we focused on the DNA protection activity of 3,5,7,3′,4′-pentamethoxyflavone (PMF), a Quercetin Derivative isolated from Kaemperia parviflora. Although, PMF was found to be a very poor antioxidant compound, still it could remarkably protect DNA from oxidative damage. DNA binding assay showed that PMF bound to the minor groove of DNA, which suggests a possible mechanism for its DNA protective effects. Cellular toxicity assay on RAW 264.7 macrophages showed this compound is very safe for therapeutic applications.
