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Shunyi Wang - One of the best experts on this subject based on the ideXlab platform.

Muralee Murugesu - One of the best experts on this subject based on the ideXlab platform.

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  • reactivity of hypotaurine and cysteine sulfinic acid toward carbonate Radical Anion and nitrogen dioxide as explored by the peroxidase activity of cu zn superoxide dismutase and by pulse radiolysis
    Free Radical Research, 2014
    Co-Authors: Baseggio A Conrado, Laura Pecci, M Dangelantonio, Armida Torreggiani, Mario Fontana
    Abstract:

    AbstractHypotaurine and cysteine sulfinic acid are known to be readily oxidized to the respective sulfonates, taurine and cysteic acid, by several oxidative agents that may be present in biological systems. In this work, the relevance of both the carbonate Anion and nitrogen dioxide Radicals in the oxidation of hypotaurine and cysteine sulfinic acid has been explored by the peroxidase activity of Cu,Zn superoxide dismutase (SOD) and by pulse radiolysis. The extent of sulfinate oxidation induced by the system SOD/H2O2 in the presence of bicarbonate (CO3•– generation), or nitrite (•NO2 generation) has been evaluated. Hypotaurine is efficiently oxidized by the carbonate Radical Anion generated by the peroxidase activity of Cu,Zn SOD. Pulse radiolysis studies have shown that the carbonate Radical Anion reacts with hypotaurine more rapidly (k = 1.1 × 109 M−1s−1) than nitrogen dioxide (k = 1.6 × 107 M−1s−1). Regarding cysteine sulfinic acid, it is less reactive with the carbonate Radical Anion (k = 5.5 × 107 M−...

  • reactivity of hypotaurine and cysteine sulfinic acid toward carbonate Radical Anion and nitrogen dioxide as explored by the peroxidase activity of cu zn superoxide dismutase and by pulse radiolysis
    Free Radical Research, 2014
    Co-Authors: Baseggio A Conrado, Laura Pecci, M Dangelantonio, Armida Torreggiani, Mario Fontana
    Abstract:

    Hypotaurine and cysteine sulfinic acid are known to be readily oxidized to the respective sulfonates, taurine and cysteic acid, by several oxidative agents that may be present in biological systems. In this work, the relevance of both the carbonate Anion and nitrogen dioxide Radicals in the oxidation of hypotaurine and cysteine sulfinic acid has been explored by the peroxidase activity of Cu,Zn superoxide dismutase (SOD) and by pulse radiolysis. The extent of sulfinate oxidation induced by the system SOD/H2O2 in the presence of bicarbonate (CO3(•-) generation), or nitrite ((•)NO2 generation) has been evaluated. Hypotaurine is efficiently oxidized by the carbonate Radical Anion generated by the peroxidase activity of Cu,Zn SOD. Pulse radiolysis studies have shown that the carbonate Radical Anion reacts with hypotaurine more rapidly (k = 1.1 × 10(9) M(-1)s(-1)) than nitrogen dioxide (k = 1.6 × 10(7) M(-1)s(-1)). Regarding cysteine sulfinic acid, it is less reactive with the carbonate Radical Anion (k = 5.5 × 10(7) M(-1)s(-1)) than hypotaurine. It has also been observed that the one-electron transfer oxidation of both sulfinates by the Radicals is accompanied by the generation of transient sulfonyl Radicals (RSO2(•)). Considering that the carbonate Radical Anion could be formed in vivo at high level from bicarbonate, this Radical can be included in the oxidants capable of performing the last metabolic step of taurine biosynthesis. Moreover, the protective effect exerted by hypotaurine and cysteine sulfinate on the carbonate Radical Anion-mediated tyrosine dimerization indicates that both sulfinates have scavenging activity towards the carbonate Radical Anion. However, the formation of transient reactive intermediates during sulfinate oxidation by carbonate Anion and nitrogen dioxide Radical may at the same time promote oxidative reactions.

Jay L Zweier - One of the best experts on this subject based on the ideXlab platform.