The Experts below are selected from a list of 63 Experts worldwide ranked by ideXlab platform

K. M. Verburg - One of the best experts on this subject based on the ideXlab platform.

  • Session 1: Radioenzymatic Assay for Histamine: Development and Validation
    Scandinavian journal of gastroenterology. Supplement, 1991
    Co-Authors: David P. Henry, Ronald R. Bowsher, K. M. Verburg
    Abstract:

    Henry DP, Bowsher RR, Verburg KM. Radioenzymatic Assay for histamine: development and validation. Scand J Gastroenterol 1991, 26(suppl 180), 26–31Radioenzymatic Assays are sensitive analytic tools that use an enzyme to quantify a substrate for that enzyme. Purified histamine N-methyltransferase has been used as the basis for an Assay for histamine. The sensitivity of the procedure is

  • session 1 Radioenzymatic Assay for histamine development and validation
    Scandinavian Journal of Gastroenterology, 1991
    Co-Authors: David P. Henry, Ronald R. Bowsher, K. M. Verburg
    Abstract:

    Henry DP, Bowsher RR, Verburg KM. Radioenzymatic Assay for histamine: development and validation. Scand J Gastroenterol 1991, 26(suppl 180), 26–31Radioenzymatic Assays are sensitive analytic tools that use an enzyme to quantify a substrate for that enzyme. Purified histamine N-methyltransferase has been used as the basis for an Assay for histamine. The sensitivity of the procedure is <10fmol. The specificity of the Assay is increased when the transferase reaction is carried out at 0–3°C. Data documenting the precision of the Assay, the stability of histamine in human plasma, and the gastric secretory rate of histamine are presented along with a chronologic description of the development of the technique.

David P. Henry - One of the best experts on this subject based on the ideXlab platform.

  • Session 1: Radioenzymatic Assay for Histamine: Development and Validation
    Scandinavian journal of gastroenterology. Supplement, 1991
    Co-Authors: David P. Henry, Ronald R. Bowsher, K. M. Verburg
    Abstract:

    Henry DP, Bowsher RR, Verburg KM. Radioenzymatic Assay for histamine: development and validation. Scand J Gastroenterol 1991, 26(suppl 180), 26–31Radioenzymatic Assays are sensitive analytic tools that use an enzyme to quantify a substrate for that enzyme. Purified histamine N-methyltransferase has been used as the basis for an Assay for histamine. The sensitivity of the procedure is

  • session 1 Radioenzymatic Assay for histamine development and validation
    Scandinavian Journal of Gastroenterology, 1991
    Co-Authors: David P. Henry, Ronald R. Bowsher, K. M. Verburg
    Abstract:

    Henry DP, Bowsher RR, Verburg KM. Radioenzymatic Assay for histamine: development and validation. Scand J Gastroenterol 1991, 26(suppl 180), 26–31Radioenzymatic Assays are sensitive analytic tools that use an enzyme to quantify a substrate for that enzyme. Purified histamine N-methyltransferase has been used as the basis for an Assay for histamine. The sensitivity of the procedure is <10fmol. The specificity of the Assay is increased when the transferase reaction is carried out at 0–3°C. Data documenting the precision of the Assay, the stability of histamine in human plasma, and the gastric secretory rate of histamine are presented along with a chronologic description of the development of the technique.

Anne E. Green - One of the best experts on this subject based on the ideXlab platform.

  • Determination of plasma free carnitine by electrospray tandem mass spectrometry.
    Annals of Clinical Biochemistry, 2001
    Co-Authors: David T Hardy, Mary Anne Preece, Anne E. Green
    Abstract:

    We have evaluated a tandem mass spectrometry method for determining free carnitine concentrations in plasma and have compared its performance with that of an existing Radioenzymatic Assay. In this method, plasma was mixed with an internal standard, carnitine-d3 (600 nmol/L), and butylated before analysis on a Quattro II tandem mass spectrometer. The detection limit of the tandem mass spectrometric (MS) method was 4 micromol/L and carryover between samples was 2.2%. Precision of the method was 1.6-5.4% between injections and 4.0-5.6% between samples. Between-batch precision was 10-13%. The method was linear up to a free carnitine concentration of 300 micromol/L and good agreement was found with the existing Radioenzymatic Assay. We conclude that the tandem MS method is a precise and robust method for the determination of free carnitine concentrations in plasma that overcomes the disadvantages of the radiochemical method.

Ronald R. Bowsher - One of the best experts on this subject based on the ideXlab platform.

  • Session 1: Radioenzymatic Assay for Histamine: Development and Validation
    Scandinavian journal of gastroenterology. Supplement, 1991
    Co-Authors: David P. Henry, Ronald R. Bowsher, K. M. Verburg
    Abstract:

    Henry DP, Bowsher RR, Verburg KM. Radioenzymatic Assay for histamine: development and validation. Scand J Gastroenterol 1991, 26(suppl 180), 26–31Radioenzymatic Assays are sensitive analytic tools that use an enzyme to quantify a substrate for that enzyme. Purified histamine N-methyltransferase has been used as the basis for an Assay for histamine. The sensitivity of the procedure is

  • session 1 Radioenzymatic Assay for histamine development and validation
    Scandinavian Journal of Gastroenterology, 1991
    Co-Authors: David P. Henry, Ronald R. Bowsher, K. M. Verburg
    Abstract:

    Henry DP, Bowsher RR, Verburg KM. Radioenzymatic Assay for histamine: development and validation. Scand J Gastroenterol 1991, 26(suppl 180), 26–31Radioenzymatic Assays are sensitive analytic tools that use an enzyme to quantify a substrate for that enzyme. Purified histamine N-methyltransferase has been used as the basis for an Assay for histamine. The sensitivity of the procedure is <10fmol. The specificity of the Assay is increased when the transferase reaction is carried out at 0–3°C. Data documenting the precision of the Assay, the stability of histamine in human plasma, and the gastric secretory rate of histamine are presented along with a chronologic description of the development of the technique.

Roger F. Butterworth - One of the best experts on this subject based on the ideXlab platform.

  • Region-selective reductions in activities of glutamine synthetase in rat brain following portacaval anastomosis
    Metabolic Brain Disease, 1993
    Co-Authors: Guylaine Girard, Jean-françois Giguère, Roger F. Butterworth
    Abstract:

    Portacaval anastomosis in the rat results in liver atrophy, sustained hyperammonemia and mild encephalopathy. Previous studies have demonstrated region-selective alterations of glutamine and other ammonia-related amino acids in brain following portacaval anastomosis. Ammonia removal by brain relies on glutamine synthesis and the enzyme responsible, glutamine synthetase, has an almost exclusively astrocytic localization. Glutamine synthetase activities were measured using a Radioenzymatic Assay in homogenates of seven brain regions of rats four weeks after end-to-side portacaval anastomosis. Enzyme activities were significantly reduced in hippocampus (by 25%, p