Raman Microscopy

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Katsumasa Fujita - One of the best experts on this subject based on the ideXlab platform.

  • Super-Resolution Imaging in Raman Microscopy
    Biological and Medical Physics Biomedical Engineering, 2019
    Co-Authors: Katsumasa Fujita
    Abstract:

    Raman Microscopy provides microscopic images of the sample with chemical information as the contrast. Since Raman Microscopy uses light–material interactions associated with vibrational excitation of molecules, the spatial resolution of the microscope is limited to the half of the wavelength used to induce the Raman effect. In this chapter, we introduce various attempts for breaking the limit of the spatial resolution in Raman Microscopy. Many of those techniques take similar approaches as super-resolution fluorescence Microscopy, where the control of excitation and emission of fluorescence is the key to break the limit. Since there are many different types of Raman Microscopy, such as spontaneous Raman scattering, coherent anti-Stokes Raman scattering, stimulated Raman scattering, and so on, various approaches are proposed for spatio-temporal manipulation of the Raman effect in micro- and nanometer scale. In this chapter, we categorize the approaches to realize super-resolution Raman imaging based on their strategies for breaking the limit and introduce the principles and the theoretical and experimental demonstrations of the techniques.

  • Improvement of spatial and spectral resolution in Raman Microscopy
    Biomedical Imaging and Sensing Conference, 2017
    Co-Authors: Katsumasa Fujita
    Abstract:

    The classical limit of the spatial resolution, known as the diffraction limit, has been overcome by the recent development of super-resolution techniques. However, the main application of the technique is limited to fluorescence imaging. In our research, we have developed two techniques to improve the spatial resolution in Raman scattering imaging. We introduced the structured illumination to improve the spatial resolution in line-illumination Raman Microscopy. We also utilized the saturation effect of coherent anti-Stokes Raman scattering to improve both spatial and spectral resolution simultaneously.

  • structured line illumination Raman Microscopy
    Nature Communications, 2015
    Co-Authors: Kozue Watanabe, Almar F Palonpon, Nicholas I Smith, Liangda Chiu, Atsushi Kasai, Hitoshi Hashimoto, Satoshi Kawata, Katsumasa Fujita
    Abstract:

    In the last couple of decades, the spatial resolution in optical Microscopy has increased to unprecedented levels by exploiting the fluorescence properties of the probe. At about the same time, Raman imaging techniques have emerged as a way to image inherent chemical information in a sample without using fluorescent probes. However, in many applications, the achievable resolution is limited to about half the wavelength of excitation light. Here we report the use of structured illumination to increase the spatial resolution of label-free spontaneous Raman Microscopy, generating highly detailed spatial contrast from the ensemble of molecular information in the sample. Using structured line illumination in slit-scanning Raman Microscopy, we demonstrate a marked improvement in spatial resolution and show the applicability to a range of samples, including both biological and inorganic chemical component mapping. This technique is expected to contribute towards greater understanding of chemical component distributions in organic and inorganic materials.

  • Enhancement of the lateral resolution of Raman Microscopy by use of structured illumination
    The Japan Society of Applied Physics, 2015
    Co-Authors: Almar F Palonpon, Kozue Watanabe, Nicholas I Smith, Liangda Chiu, Atsushi Kasai, Hitoshi Hashimoto, Satoshi Kawata, Katsumasa Fujita
    Abstract:

    Recent developments in optical Microscopy especially fluorescence Microscopy have pushed the limits of spatial resolution below the diffraction limit, to as low as several nanometers. Most of these super-resolution techniques, however, cannot be adapted to Raman Microscopy because their working principle is based on manipulating the fluorescence emission properties of the sample. On the other hand, structured illumination Microscopy (SIM) utilizes the Moire effect (interference of the structured light pattern with the sample structure) to double the spatial resolution [1] and therefore does not impose particular optical properties on the sample, making it a feasible technique for Raman Microscopy.

  • Visualization of lipid rafts in an artificial monolayer membrane by using slit-scanning Raman Microscopy
    The Japan Society of Applied Physics, 2015
    Co-Authors: Jun Ando, Katsumasa Fujita, Hiroyuki Yamakoshi, Kosuke Dodo, Masanao Kinoshita, Michio Murata, Mikiko Sodeoka
    Abstract:

    Raman scattering Microscopy identifies molecular species in a sample by optically detecting molecular vibration, and provides spatial distribution of molecules in a specimen. Our developed slit-scanning Raman Microscopy improved imaging speed more than 100 times faster than that of conventional point-scanning Raman Microscopy, by providing both diffraction-limited high spatial resolution and chemical information. It allows us to observe dynamic behavior of biomolecules in a living cell during various biological processes, such as cytokinesis, mitosis, and apoptosis [1,2].

Frank W. Wise - One of the best experts on this subject based on the ideXlab platform.

Robin J H Clark - One of the best experts on this subject based on the ideXlab platform.

  • Raman Microscopy in art history and conservation science
    Studies in Conservation, 2020
    Co-Authors: Gregory D Smith, Robin J H Clark
    Abstract:

    Numerous applications of Raman Microscopy in art history and conservation science have appeared in the literature, but unfortunately this work has gone largely unrecognized by practitioners in those fields. This article assesses the causes of this situation and seeks to inform conservators, art historians and archaeologists of the role that Raman Microscopy is playing in the analysis of historical materials. A brief description of the Raman scattering phenomenon and the instrumentation used to collect Raman spectra is presented, and the capabilities and limitations of the technique are discussed. Importantly, a comprehensive and critical review is provided of Raman Microscopy applications in the technical analysis of art and artefacts.

  • The scientific investigation of artwork and archaeological artefacts: Raman Microscopy as a structural, analytical and forensic tool
    Applied Physics A, 2007
    Co-Authors: Robin J H Clark
    Abstract:

    The impact of Raman Microscopy on the study of artwork and archaeological artefacts is outlined. Important recent case studies are presented to illustrate the power of the technique to answer – either alone or in conjunction with other techniques – key questions of great art historical, conservational, cultural, archaeological and scientific interest. Raman Microscopy also offers, via pigment identification, the possibility of testing the attribution of artwork, a matter which should be of great concern to galleries, auction houses and museums.

  • Raman Microscopy in archaeological science
    Journal of Archaeological Science, 2004
    Co-Authors: Gregory D Smith, Robin J H Clark
    Abstract:

    Improvements in the instrumentation for dispersive and interferometric Fourier transform (FT) Raman Microscopy have overcome many of the earlier limitations of these techniques, thus opening the way for their widespread and routine utilisation in archaeometry laboratories. The use of Raman spectroscopy for identifying and studying archaeological materials has flourished in recent years, but the resulting articles have seldom been published in the archaeology literature, thus limiting their impact on the field. Therefore, this article covers concisely the theory and instrumentation of Raman Microscopy and then comprehensively reviews the many applications of this technique in archaeometric research. The significant advances made in archaeological science through the use of Raman Microscopy are highlighted, but many areas requiring further research, such as the generation of more extensive and reliable spectral libraries and the surmounting of obstacles in the analysis of certain classes of historical materials, are also revealed.

  • Pigment identification in paintings employing laser induced breakdown spectroscopy and Raman Microscopy
    Spectrochimica Acta Part B: Atomic Spectroscopy, 2001
    Co-Authors: Lucia Burgio, Robin J H Clark, Kristalia Melessanaki, Michael Doulgeridis, D. Anglos
    Abstract:

    Abstract Laser-induced breakdown spectroscopy (LIBS) was used in combination with Raman Microscopy, for the identification of pigments in different types of painted works of art. More specifically, a 19th century post-Byzantine icon from Greece and two miniature paintings from France were examined and detailed spectral data are presented which lead to the identification of the pigments used. LIBS measurements yielded information on the presence of pigments or mixtures of pigments based on the characteristic emission from specific elements. Identification of most pigments was performed by Raman Microscopy. As demonstrated in this work, the combined use of LIBS and Raman Microscopy, two complementary techniques, leads to a detailed characterization of the paintings examined with respect to the pigments used.

  • Laser induced breakdown spectroscopy and Raman Microscopy for analysis of pigments in polychromes
    Journal of Cultural Heritage, 2000
    Co-Authors: Marta Castillejo, Lucia Burgio, D. Anglos, Margarita Martín, Diego Furtado Silva, T. Stratoudaki, Robin J H Clark
    Abstract:

    Abstract A polychrome from the Rococo period was analysed by use of two laser-based analytical techniques, laser-induced breakdown spectroscopy (LIBS) and Raman Microscopy. The analysis, performed on a fragment of a gilded altarpiece from the church of Escatron, Zaragoza, Spain, provided detailed spectral data that have been used for the identification of pigments. LIBS measurements yielded elemental analytical data that suggest the presence of certain pigments and, in addition, provide information on the stratigraphy of the paint layers. Identification of most pigments and of the materials used in the preparation layer was performed by Raman Microscopy.

Sergei G Kazarian - One of the best experts on this subject based on the ideXlab platform.

Oliver S. Fleming - One of the best experts on this subject based on the ideXlab platform.