The Experts below are selected from a list of 237 Experts worldwide ranked by ideXlab platform
Renato Zenobi - One of the best experts on this subject based on the ideXlab platform.
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minimizing ion competition boosts volatile metabolome coverage by secondary electrospray ionization orbitrap mass spectrometry
Analytica Chimica Acta, 2021Co-Authors: Jiayi Lan, Jerome Kaeslin, Giorgia Greter, Renato ZenobiAbstract:Abstract Secondary electrospray ionization high-resolution mass spectrometry (SESI-HRMS) is an emerging technique for the detection of volatile metabolites. However, sensitivity and reproducibility of SESI-HRMS have limited its applications in untargeted metabolomics profiling. Ion suppression in the SESI source has been considered to be the main cause. Here, we show that besides ion suppression, ion competition in the C-trap of Orbitrap instruments is another important factor that influences sensitivity and reproducibility of SESI-MS. Instead of acquiring the full mass-to-charge ratio (m/z) Range, acquisition of consecutive m/z windows to minimize the ion competition effect allows the detection of more features. m/z window Ranges are optimized to fill the C-trap either with an equal number of features or an equal cumulative intensity per window. Considering a balance between maximizing scanning speed and minimizing ion competition, splitting the m/z = 50-500 Range into 4 windows is selected for measuring human breath and bacterial culture samples on SESI-Orbitrap MS, corresponding to a duty cycle of 2.3 s at a resolution of 140’000. In a small cohort of human subjects, the proposed splitting into 4 windows allows three times more features to be detected compared to the classical full m/z Range Method.
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minimizing ion competition boosts volatile metabolome analysis by secondary electrospray ionization orbitrap mass spectrometry
bioRxiv, 2020Co-Authors: Jerome Kaeslin, Jiayi Lan, Renato ZenobiAbstract:Secondary electrospray ionization high-resolution mass spectrometry (SESI-HRMS) is an emerging technique for the detection of volatile metabolites. However, sensitivity and reproducibility of SESI-HRMS have limited its applications in untargeted metabolomics profiling. Ion suppression in the SESI source has been considered to be the main cause. Here, we show that besides ion suppression, ion competition in the C-trap of Orbitrap instruments is another important factor that influences sensitivity and reproducibility of SESI-MS. Instead of acquiring the full mass-to-charge ratio (m/z) Range, acquisition of consecutive m/z windows to minimize the ion competition effect allows the detection of more features. m=z window Ranges are optimized to fill the C-trap either with an equal number of features or an equal cumulative intensity per window. Considering a balance between maximizing scanning speed and minimizing ion competition, splitting the m/z = 50-500 Range into 4 windows is selected for measuring human breath and bacterial culture samples on SESI-Orbitrap MS, corresponding to a duty cycle of 2:3 s at a resolution of 140000. In a small cohort of human subjects, the proposed splitting into 4 windows allows three times more features to be detected compared to the classical full m/z Range Method.
Jiayi Lan - One of the best experts on this subject based on the ideXlab platform.
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minimizing ion competition boosts volatile metabolome coverage by secondary electrospray ionization orbitrap mass spectrometry
Analytica Chimica Acta, 2021Co-Authors: Jiayi Lan, Jerome Kaeslin, Giorgia Greter, Renato ZenobiAbstract:Abstract Secondary electrospray ionization high-resolution mass spectrometry (SESI-HRMS) is an emerging technique for the detection of volatile metabolites. However, sensitivity and reproducibility of SESI-HRMS have limited its applications in untargeted metabolomics profiling. Ion suppression in the SESI source has been considered to be the main cause. Here, we show that besides ion suppression, ion competition in the C-trap of Orbitrap instruments is another important factor that influences sensitivity and reproducibility of SESI-MS. Instead of acquiring the full mass-to-charge ratio (m/z) Range, acquisition of consecutive m/z windows to minimize the ion competition effect allows the detection of more features. m/z window Ranges are optimized to fill the C-trap either with an equal number of features or an equal cumulative intensity per window. Considering a balance between maximizing scanning speed and minimizing ion competition, splitting the m/z = 50-500 Range into 4 windows is selected for measuring human breath and bacterial culture samples on SESI-Orbitrap MS, corresponding to a duty cycle of 2.3 s at a resolution of 140’000. In a small cohort of human subjects, the proposed splitting into 4 windows allows three times more features to be detected compared to the classical full m/z Range Method.
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minimizing ion competition boosts volatile metabolome analysis by secondary electrospray ionization orbitrap mass spectrometry
bioRxiv, 2020Co-Authors: Jerome Kaeslin, Jiayi Lan, Renato ZenobiAbstract:Secondary electrospray ionization high-resolution mass spectrometry (SESI-HRMS) is an emerging technique for the detection of volatile metabolites. However, sensitivity and reproducibility of SESI-HRMS have limited its applications in untargeted metabolomics profiling. Ion suppression in the SESI source has been considered to be the main cause. Here, we show that besides ion suppression, ion competition in the C-trap of Orbitrap instruments is another important factor that influences sensitivity and reproducibility of SESI-MS. Instead of acquiring the full mass-to-charge ratio (m/z) Range, acquisition of consecutive m/z windows to minimize the ion competition effect allows the detection of more features. m=z window Ranges are optimized to fill the C-trap either with an equal number of features or an equal cumulative intensity per window. Considering a balance between maximizing scanning speed and minimizing ion competition, splitting the m/z = 50-500 Range into 4 windows is selected for measuring human breath and bacterial culture samples on SESI-Orbitrap MS, corresponding to a duty cycle of 2:3 s at a resolution of 140000. In a small cohort of human subjects, the proposed splitting into 4 windows allows three times more features to be detected compared to the classical full m/z Range Method.
Jerome Kaeslin - One of the best experts on this subject based on the ideXlab platform.
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minimizing ion competition boosts volatile metabolome coverage by secondary electrospray ionization orbitrap mass spectrometry
Analytica Chimica Acta, 2021Co-Authors: Jiayi Lan, Jerome Kaeslin, Giorgia Greter, Renato ZenobiAbstract:Abstract Secondary electrospray ionization high-resolution mass spectrometry (SESI-HRMS) is an emerging technique for the detection of volatile metabolites. However, sensitivity and reproducibility of SESI-HRMS have limited its applications in untargeted metabolomics profiling. Ion suppression in the SESI source has been considered to be the main cause. Here, we show that besides ion suppression, ion competition in the C-trap of Orbitrap instruments is another important factor that influences sensitivity and reproducibility of SESI-MS. Instead of acquiring the full mass-to-charge ratio (m/z) Range, acquisition of consecutive m/z windows to minimize the ion competition effect allows the detection of more features. m/z window Ranges are optimized to fill the C-trap either with an equal number of features or an equal cumulative intensity per window. Considering a balance between maximizing scanning speed and minimizing ion competition, splitting the m/z = 50-500 Range into 4 windows is selected for measuring human breath and bacterial culture samples on SESI-Orbitrap MS, corresponding to a duty cycle of 2.3 s at a resolution of 140’000. In a small cohort of human subjects, the proposed splitting into 4 windows allows three times more features to be detected compared to the classical full m/z Range Method.
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minimizing ion competition boosts volatile metabolome analysis by secondary electrospray ionization orbitrap mass spectrometry
bioRxiv, 2020Co-Authors: Jerome Kaeslin, Jiayi Lan, Renato ZenobiAbstract:Secondary electrospray ionization high-resolution mass spectrometry (SESI-HRMS) is an emerging technique for the detection of volatile metabolites. However, sensitivity and reproducibility of SESI-HRMS have limited its applications in untargeted metabolomics profiling. Ion suppression in the SESI source has been considered to be the main cause. Here, we show that besides ion suppression, ion competition in the C-trap of Orbitrap instruments is another important factor that influences sensitivity and reproducibility of SESI-MS. Instead of acquiring the full mass-to-charge ratio (m/z) Range, acquisition of consecutive m/z windows to minimize the ion competition effect allows the detection of more features. m=z window Ranges are optimized to fill the C-trap either with an equal number of features or an equal cumulative intensity per window. Considering a balance between maximizing scanning speed and minimizing ion competition, splitting the m/z = 50-500 Range into 4 windows is selected for measuring human breath and bacterial culture samples on SESI-Orbitrap MS, corresponding to a duty cycle of 2:3 s at a resolution of 140000. In a small cohort of human subjects, the proposed splitting into 4 windows allows three times more features to be detected compared to the classical full m/z Range Method.
M. Stoschka - One of the best experts on this subject based on the ideXlab platform.
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3rd International Conference on Material and Component Performance under Variable Amplitude Loading, VAL2015 Fatigue strength of HFMI-treated high-strength steel joints under constant and variable amplitude block loading
2015Co-Authors: M. Leitner, S. Gerstbrein, M. StoschkaAbstract:Lightweight-design of welded high-strength steel structures in cyclic service necessitates the use of post-treatment Methods like the high frequency mechanical impact treatment (HFMI). Service loads during operation mostly consist of variable amplitudes, whereat recommendations are only available for the as-welded condition. Therefore, this paper deals with the effect of variable amplitude block loading on the fatigue strength of HFMI-treated T-joints. An evaluation of the real damage sum exhibits characteristic distinctions to constant amplitude test results in regard to the base material strength. The application of an equivalent stress Range Method by nominal and effective notch stress approach is finally presented.
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Fatigue Strength of HFMI-treated High-strength Steel Joints under Constant and Variable Amplitude Block Loading
Procedia Engineering, 2015Co-Authors: M. Leitner, S. Gerstbrein, Markus Ottersböck, M. StoschkaAbstract:Abstract Lightweight-design of welded high-strength steel structures in cyclic service necessitates the use of post-treatment Methods like the high frequency mechanical impact treatment (HFMI). Service loads during operation mostly consist of variable amplitudes, whereat recommendations are only available for the as-welded condition. Therefore, this paper deals with the effect of variable amplitude block loading on the fatigue strength of HFMI-treated T-joints. An evaluation of the real damage sum exhibits characteristic distinctions to constant amplitude test results in regard to the base material strength. The application of an equivalent stress Range Method by nominal and effective notch stress approach is finally presented.
Giorgia Greter - One of the best experts on this subject based on the ideXlab platform.
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minimizing ion competition boosts volatile metabolome coverage by secondary electrospray ionization orbitrap mass spectrometry
Analytica Chimica Acta, 2021Co-Authors: Jiayi Lan, Jerome Kaeslin, Giorgia Greter, Renato ZenobiAbstract:Abstract Secondary electrospray ionization high-resolution mass spectrometry (SESI-HRMS) is an emerging technique for the detection of volatile metabolites. However, sensitivity and reproducibility of SESI-HRMS have limited its applications in untargeted metabolomics profiling. Ion suppression in the SESI source has been considered to be the main cause. Here, we show that besides ion suppression, ion competition in the C-trap of Orbitrap instruments is another important factor that influences sensitivity and reproducibility of SESI-MS. Instead of acquiring the full mass-to-charge ratio (m/z) Range, acquisition of consecutive m/z windows to minimize the ion competition effect allows the detection of more features. m/z window Ranges are optimized to fill the C-trap either with an equal number of features or an equal cumulative intensity per window. Considering a balance between maximizing scanning speed and minimizing ion competition, splitting the m/z = 50-500 Range into 4 windows is selected for measuring human breath and bacterial culture samples on SESI-Orbitrap MS, corresponding to a duty cycle of 2.3 s at a resolution of 140’000. In a small cohort of human subjects, the proposed splitting into 4 windows allows three times more features to be detected compared to the classical full m/z Range Method.
