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Aleksandra Grudniewska - One of the best experts on this subject based on the ideXlab platform.

  • enhanced protein extraction from oilseed Cakes using glycerol choline chloride deep eutectic solvents a biorefinery approach
    ACS Sustainable Chemistry & Engineering, 2018
    Co-Authors: Aleksandra Grudniewska, Filip Boratynski, Radoslaw Gnilka, Eduardo M De Melo, Angel Chan, Avtar S Matharu
    Abstract:

    The enhanced extraction of proteins from Rapeseed Cake (RC) and evening primrose Cake (EC) using a glycerol–choline chloride deep eutectic solvent (DES, glyceline) is reported. Protein-rich precipitates were obtained by adding water (antisolvent) to the DES extract derived at different processing temperatures. The presence of proteins in precipitates has been confirmed by several techniques, such as NMR, ATR-IR, TGA, CHN, and SDS-PAGE. Yield of precipitates improved with increasing temperature of treatment, reaching a maximum of 20% and 35% at 140 °C from RC and EC, respectively. In general, the protein content of the extracts was ca. 40–50%, which is up to 20% more than the starting materials. SDS-PAGE confirmed that glyceline selectively extracted cruciferin proteins (ca. 16–33 kDa) from RC, while proteins with variable molecular weight (10–40 kDa) were identified in EC extracts. As a potential application, cruciferin-rich RCP60 and RCP100 could be incorporated into final food formulations as a source o...

  • improving of hydrolases biosythesis by solid state fermentation of penicillium camemberti on Rapeseed Cake
    Scientific Reports, 2018
    Co-Authors: Filip Boratynski, Ewa Szczepanska, Aleksandra Grudniewska, Radoslaw Gnilka, Teresa Olejniczak
    Abstract:

    The study show usefulness of Rapeseed Cake, rich in fats and proteins byproduct generated after oil production, which may be used as a microbial medium for lipase and protease biosynthesis. Of 26 different filamentous fungi screened by solid-state fermentation, Penicillium camemberti AM83 was found to abundantly produce lipase and protease. Various process parameters were then optimized to maximize lipase and protease secretion, including carbon and nitrogen source, C/N ratio, metal ions, temperature, moisture content, initial pH, and inoculum size. Lipase production increased approximately 11.2-fold in solid-state cultures on Rapeseed Cake supplemented with lactose and calcium chloride, alkalinized to pH 8, hydrated to 80%, and inoculated with 1.2 × 106 spores/mL. Similarly, protease production increased approximately 8.4-fold in optimized cultures inoculated with 3.2 × 108 spores/mL, and grown on Rapeseed Cake with lactose and ammonium sulfate at pH 9 and moisture content 60%. The results highlight the potential economic value of solid-state fermentation on Rapeseed Cake to produce industrial hydrolases.

Avtar S Matharu - One of the best experts on this subject based on the ideXlab platform.

  • enhanced protein extraction from oilseed Cakes using glycerol choline chloride deep eutectic solvents a biorefinery approach
    ACS Sustainable Chemistry & Engineering, 2018
    Co-Authors: Aleksandra Grudniewska, Filip Boratynski, Radoslaw Gnilka, Eduardo M De Melo, Angel Chan, Avtar S Matharu
    Abstract:

    The enhanced extraction of proteins from Rapeseed Cake (RC) and evening primrose Cake (EC) using a glycerol–choline chloride deep eutectic solvent (DES, glyceline) is reported. Protein-rich precipitates were obtained by adding water (antisolvent) to the DES extract derived at different processing temperatures. The presence of proteins in precipitates has been confirmed by several techniques, such as NMR, ATR-IR, TGA, CHN, and SDS-PAGE. Yield of precipitates improved with increasing temperature of treatment, reaching a maximum of 20% and 35% at 140 °C from RC and EC, respectively. In general, the protein content of the extracts was ca. 40–50%, which is up to 20% more than the starting materials. SDS-PAGE confirmed that glyceline selectively extracted cruciferin proteins (ca. 16–33 kDa) from RC, while proteins with variable molecular weight (10–40 kDa) were identified in EC extracts. As a potential application, cruciferin-rich RCP60 and RCP100 could be incorporated into final food formulations as a source o...

Farid Chemat - One of the best experts on this subject based on the ideXlab platform.

  • green sonoextraction of protein from oleaginous press Rapeseed Cake
    Molecules, 2017
    Co-Authors: Meryem Boukroufa, Anne Gaëlle Sicaire, Christian Larre, Njara Rakotomanomana, Frédéric Fine, Aude Le Goff, Veronique Sole Jamault, Farid Chemat
    Abstract:

    In this study, extraction of soluble proteins from Rapeseed Cake using different conventional and innovative extraction processes in order to maximize the extraction yield has been investigated. Firstly, various extraction techniques including ultrasound, microwave, and percolation were tested to increase the protein recovery efficiency. Secondly, response surface methodology (RSM) using a central composite design (CCD) approach was applied to investigate the influence of process variables on ultrasound-assisted extraction (UAE). Statistical analysis revealed that the optimized conditions providing a protein yield of 4.24 g/100 g DM were an ultrasound power of 5.6 W·cm−2 and temperature of 45 °C. Quantitatively UAE followed by two stages of conventional extraction gave the best total protein yield of 9.81 g/100 g DM. Qualitatively, the protein efficiency ratio (PER) used as measure of the nutritive value (12S/2S ratio) which indicates protein quality in terms of S-containing essential amino acids, was similar to that of the conventional extraction method. Small amounts of protein aggregate were observed in the HPLC profile of the extract.

  • Green sonoextraction of protein from oleaginous press Rapeseed Cake
    Molecules, 2017
    Co-Authors: Meryem Boukroufa, Anne Gaëlle Sicaire, Véronique Sole, Christian Larre, Njara Rakotomanomana, Frédéric Fine, Alan Le Goff, Farid Chemat
    Abstract:

    In this study, extraction of soluble proteins from Rapeseed Cake using different conventional and innovative extraction processes in order to maximize the extraction yield has been investigated. Firstly, various extraction techniques including ultrasound, microwave, and percolation were tested to increase the protein recovery efficiency. Secondly, response surface methodology (RSM) using a central composite design (CCD) approach was applied to investigate the influence of process variables on ultrasound-assisted extraction (UAE). Statistical analysis revealed that the optimized conditions providing a protein yield of 4.24 g/100 g DM were an ultrasound power of 5.6 W cm.

Filip Boratynski - One of the best experts on this subject based on the ideXlab platform.

  • enhanced protein extraction from oilseed Cakes using glycerol choline chloride deep eutectic solvents a biorefinery approach
    ACS Sustainable Chemistry & Engineering, 2018
    Co-Authors: Aleksandra Grudniewska, Filip Boratynski, Radoslaw Gnilka, Eduardo M De Melo, Angel Chan, Avtar S Matharu
    Abstract:

    The enhanced extraction of proteins from Rapeseed Cake (RC) and evening primrose Cake (EC) using a glycerol–choline chloride deep eutectic solvent (DES, glyceline) is reported. Protein-rich precipitates were obtained by adding water (antisolvent) to the DES extract derived at different processing temperatures. The presence of proteins in precipitates has been confirmed by several techniques, such as NMR, ATR-IR, TGA, CHN, and SDS-PAGE. Yield of precipitates improved with increasing temperature of treatment, reaching a maximum of 20% and 35% at 140 °C from RC and EC, respectively. In general, the protein content of the extracts was ca. 40–50%, which is up to 20% more than the starting materials. SDS-PAGE confirmed that glyceline selectively extracted cruciferin proteins (ca. 16–33 kDa) from RC, while proteins with variable molecular weight (10–40 kDa) were identified in EC extracts. As a potential application, cruciferin-rich RCP60 and RCP100 could be incorporated into final food formulations as a source o...

  • improving of hydrolases biosythesis by solid state fermentation of penicillium camemberti on Rapeseed Cake
    Scientific Reports, 2018
    Co-Authors: Filip Boratynski, Ewa Szczepanska, Aleksandra Grudniewska, Radoslaw Gnilka, Teresa Olejniczak
    Abstract:

    The study show usefulness of Rapeseed Cake, rich in fats and proteins byproduct generated after oil production, which may be used as a microbial medium for lipase and protease biosynthesis. Of 26 different filamentous fungi screened by solid-state fermentation, Penicillium camemberti AM83 was found to abundantly produce lipase and protease. Various process parameters were then optimized to maximize lipase and protease secretion, including carbon and nitrogen source, C/N ratio, metal ions, temperature, moisture content, initial pH, and inoculum size. Lipase production increased approximately 11.2-fold in solid-state cultures on Rapeseed Cake supplemented with lactose and calcium chloride, alkalinized to pH 8, hydrated to 80%, and inoculated with 1.2 × 106 spores/mL. Similarly, protease production increased approximately 8.4-fold in optimized cultures inoculated with 3.2 × 108 spores/mL, and grown on Rapeseed Cake with lactose and ammonium sulfate at pH 9 and moisture content 60%. The results highlight the potential economic value of solid-state fermentation on Rapeseed Cake to produce industrial hydrolases.

Radoslaw Gnilka - One of the best experts on this subject based on the ideXlab platform.

  • enhanced protein extraction from oilseed Cakes using glycerol choline chloride deep eutectic solvents a biorefinery approach
    ACS Sustainable Chemistry & Engineering, 2018
    Co-Authors: Aleksandra Grudniewska, Filip Boratynski, Radoslaw Gnilka, Eduardo M De Melo, Angel Chan, Avtar S Matharu
    Abstract:

    The enhanced extraction of proteins from Rapeseed Cake (RC) and evening primrose Cake (EC) using a glycerol–choline chloride deep eutectic solvent (DES, glyceline) is reported. Protein-rich precipitates were obtained by adding water (antisolvent) to the DES extract derived at different processing temperatures. The presence of proteins in precipitates has been confirmed by several techniques, such as NMR, ATR-IR, TGA, CHN, and SDS-PAGE. Yield of precipitates improved with increasing temperature of treatment, reaching a maximum of 20% and 35% at 140 °C from RC and EC, respectively. In general, the protein content of the extracts was ca. 40–50%, which is up to 20% more than the starting materials. SDS-PAGE confirmed that glyceline selectively extracted cruciferin proteins (ca. 16–33 kDa) from RC, while proteins with variable molecular weight (10–40 kDa) were identified in EC extracts. As a potential application, cruciferin-rich RCP60 and RCP100 could be incorporated into final food formulations as a source o...

  • improving of hydrolases biosythesis by solid state fermentation of penicillium camemberti on Rapeseed Cake
    Scientific Reports, 2018
    Co-Authors: Filip Boratynski, Ewa Szczepanska, Aleksandra Grudniewska, Radoslaw Gnilka, Teresa Olejniczak
    Abstract:

    The study show usefulness of Rapeseed Cake, rich in fats and proteins byproduct generated after oil production, which may be used as a microbial medium for lipase and protease biosynthesis. Of 26 different filamentous fungi screened by solid-state fermentation, Penicillium camemberti AM83 was found to abundantly produce lipase and protease. Various process parameters were then optimized to maximize lipase and protease secretion, including carbon and nitrogen source, C/N ratio, metal ions, temperature, moisture content, initial pH, and inoculum size. Lipase production increased approximately 11.2-fold in solid-state cultures on Rapeseed Cake supplemented with lactose and calcium chloride, alkalinized to pH 8, hydrated to 80%, and inoculated with 1.2 × 106 spores/mL. Similarly, protease production increased approximately 8.4-fold in optimized cultures inoculated with 3.2 × 108 spores/mL, and grown on Rapeseed Cake with lactose and ammonium sulfate at pH 9 and moisture content 60%. The results highlight the potential economic value of solid-state fermentation on Rapeseed Cake to produce industrial hydrolases.