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Richard L Lieber - One of the best experts on this subject based on the ideXlab platform.

  • Sarcomere length measurement permits high resolution normalization of muscle Fiber length in architectural studies
    Journal of Experimental Biology, 2005
    Co-Authors: Amanda Felder, Samuel R Ward, Richard L Lieber
    Abstract:

    SUMMARY The use of sarcomere length to normalize Fiber length in architectural studies is commonly practiced but has not been explicitly validated. Using mouse hindlimb muscles as a model system, ankle joints were intentionally set to angles ranging from 30° to 150° and their muscles fixed. Tibialis anterior (TA), extensor digitorum longus (EDL) and soleus muscles were removed and their Raw Fiber length measured. Sarcomere length was then measured for each Fiber length sample and Fiber length was normalized to a standard sarcomere length. As expected, Raw Fiber length was dependent on tibiotarsal angle ( P r 2 range 0.22–0.61), while sarcomere length normalization eliminated the joint-angle dependent variation in Fiber length ( P >0.24, r 2 range 0.001–0.028). Similarly, one-way ANOVA revealed no significant differences in normalized Fiber length among ankle angles for any of the three muscles ( P >0.1), regardless of animal size. To determine the resolution of the method, power calculations were performed. For all muscles studied, there was >90% chance of detecting a 15% Fiber length difference among muscles and >60% chance of detecting Fiber length differences as small as 10%. We thus conclude that the use of sarcomere length normalization in architectural studies permits resolution of Fiber length variations of 15% and may even be effective at resolving 10% Fiber length variations.

  • Sarcomere length measurement permits high resolution normalization of muscle Fiber length in architectural studies.
    The Journal of experimental biology, 2005
    Co-Authors: Amanda Felder, Samuel R Ward, Richard L Lieber
    Abstract:

    The use of sarcomere length to normalize Fiber length in architectural studies is commonly practiced but has not been explicitly validated. Using mouse hindlimb muscles as a model system, ankle joints were intentionally set to angles ranging from 30 degrees to 150 degrees and their muscles fixed. Tibialis anterior (TA), extensor digitorum longus (EDL) and soleus muscles were removed and their Raw Fiber length measured. Sarcomere length was then measured for each Fiber length sample and Fiber length was normalized to a standard sarcomere length. As expected, Raw Fiber length was dependent on tibiotarsal angle (P < 0.0005 for all muscles, r2 range 0.22-0.61), while sarcomere length normalization eliminated the joint-angle dependent variation in Fiber length (P > 0.24, r2 range 0.001-0.028). Similarly, one-way ANOVA revealed no significant differences in normalized Fiber length among ankle angles for any of the three muscles (P > 0.1), regardless of animal size. To determine the resolution of the method, power calculations were performed. For all muscles studied, there was >90% chance of detecting a 15% Fiber length difference among muscles and >60% chance of detecting Fiber length differences as small as 10%. We thus conclude that the use of sarcomere length normalization in architectural studies permits resolution of Fiber length variations of 15% and may even be effective at resolving 10% Fiber length variations.

Amanda Felder - One of the best experts on this subject based on the ideXlab platform.

  • Sarcomere length measurement permits high resolution normalization of muscle Fiber length in architectural studies
    Journal of Experimental Biology, 2005
    Co-Authors: Amanda Felder, Samuel R Ward, Richard L Lieber
    Abstract:

    SUMMARY The use of sarcomere length to normalize Fiber length in architectural studies is commonly practiced but has not been explicitly validated. Using mouse hindlimb muscles as a model system, ankle joints were intentionally set to angles ranging from 30° to 150° and their muscles fixed. Tibialis anterior (TA), extensor digitorum longus (EDL) and soleus muscles were removed and their Raw Fiber length measured. Sarcomere length was then measured for each Fiber length sample and Fiber length was normalized to a standard sarcomere length. As expected, Raw Fiber length was dependent on tibiotarsal angle ( P r 2 range 0.22–0.61), while sarcomere length normalization eliminated the joint-angle dependent variation in Fiber length ( P >0.24, r 2 range 0.001–0.028). Similarly, one-way ANOVA revealed no significant differences in normalized Fiber length among ankle angles for any of the three muscles ( P >0.1), regardless of animal size. To determine the resolution of the method, power calculations were performed. For all muscles studied, there was >90% chance of detecting a 15% Fiber length difference among muscles and >60% chance of detecting Fiber length differences as small as 10%. We thus conclude that the use of sarcomere length normalization in architectural studies permits resolution of Fiber length variations of 15% and may even be effective at resolving 10% Fiber length variations.

  • Sarcomere length measurement permits high resolution normalization of muscle Fiber length in architectural studies.
    The Journal of experimental biology, 2005
    Co-Authors: Amanda Felder, Samuel R Ward, Richard L Lieber
    Abstract:

    The use of sarcomere length to normalize Fiber length in architectural studies is commonly practiced but has not been explicitly validated. Using mouse hindlimb muscles as a model system, ankle joints were intentionally set to angles ranging from 30 degrees to 150 degrees and their muscles fixed. Tibialis anterior (TA), extensor digitorum longus (EDL) and soleus muscles were removed and their Raw Fiber length measured. Sarcomere length was then measured for each Fiber length sample and Fiber length was normalized to a standard sarcomere length. As expected, Raw Fiber length was dependent on tibiotarsal angle (P < 0.0005 for all muscles, r2 range 0.22-0.61), while sarcomere length normalization eliminated the joint-angle dependent variation in Fiber length (P > 0.24, r2 range 0.001-0.028). Similarly, one-way ANOVA revealed no significant differences in normalized Fiber length among ankle angles for any of the three muscles (P > 0.1), regardless of animal size. To determine the resolution of the method, power calculations were performed. For all muscles studied, there was >90% chance of detecting a 15% Fiber length difference among muscles and >60% chance of detecting Fiber length differences as small as 10%. We thus conclude that the use of sarcomere length normalization in architectural studies permits resolution of Fiber length variations of 15% and may even be effective at resolving 10% Fiber length variations.

José Manoel Marconcini - One of the best experts on this subject based on the ideXlab platform.

  • Effect of Raw and chemically treated oil palm mesocarp Fibers on thermoplastic cassava starch properties
    Industrial Crops and Products, 2018
    Co-Authors: Adriana De Campos, A. R. Sena Neto, V.b. Rodrigues, B. R. Luchesi, Luiz H. C. Mattoso, José Manoel Marconcini
    Abstract:

    Abstract Composites of thermoplastic cassava starch (TPS) and oil palm mesocarp Fibers (OPMF) were prepared using a screw extrusion rheometer. Two types of OPMF were used: Raw and alkaline treated oil palm Fibers. TPS composites using Raw Fiber showed an improvement of 193% in the elastic modulus and 153% for maximum stress, while the elongation at break was kept constant when compared to the neat TPS matrix. The high improvement in the mechanical and thermal properties of the TPS matrix by the Raw Fiber is due to the presence of silica, which influences the interaction of the matrix and OPMF Fibers. The present work shows that TPS composites with 10 wt% Raw Fiber have greater mechanical properties than higher OPMF Raw Fiber content or alkaline treated Fibers and provides the use of OPMF residue to produce an eco-friendly composite for various applications.

Samuel R Ward - One of the best experts on this subject based on the ideXlab platform.

  • Sarcomere length measurement permits high resolution normalization of muscle Fiber length in architectural studies
    Journal of Experimental Biology, 2005
    Co-Authors: Amanda Felder, Samuel R Ward, Richard L Lieber
    Abstract:

    SUMMARY The use of sarcomere length to normalize Fiber length in architectural studies is commonly practiced but has not been explicitly validated. Using mouse hindlimb muscles as a model system, ankle joints were intentionally set to angles ranging from 30° to 150° and their muscles fixed. Tibialis anterior (TA), extensor digitorum longus (EDL) and soleus muscles were removed and their Raw Fiber length measured. Sarcomere length was then measured for each Fiber length sample and Fiber length was normalized to a standard sarcomere length. As expected, Raw Fiber length was dependent on tibiotarsal angle ( P r 2 range 0.22–0.61), while sarcomere length normalization eliminated the joint-angle dependent variation in Fiber length ( P >0.24, r 2 range 0.001–0.028). Similarly, one-way ANOVA revealed no significant differences in normalized Fiber length among ankle angles for any of the three muscles ( P >0.1), regardless of animal size. To determine the resolution of the method, power calculations were performed. For all muscles studied, there was >90% chance of detecting a 15% Fiber length difference among muscles and >60% chance of detecting Fiber length differences as small as 10%. We thus conclude that the use of sarcomere length normalization in architectural studies permits resolution of Fiber length variations of 15% and may even be effective at resolving 10% Fiber length variations.

  • Sarcomere length measurement permits high resolution normalization of muscle Fiber length in architectural studies.
    The Journal of experimental biology, 2005
    Co-Authors: Amanda Felder, Samuel R Ward, Richard L Lieber
    Abstract:

    The use of sarcomere length to normalize Fiber length in architectural studies is commonly practiced but has not been explicitly validated. Using mouse hindlimb muscles as a model system, ankle joints were intentionally set to angles ranging from 30 degrees to 150 degrees and their muscles fixed. Tibialis anterior (TA), extensor digitorum longus (EDL) and soleus muscles were removed and their Raw Fiber length measured. Sarcomere length was then measured for each Fiber length sample and Fiber length was normalized to a standard sarcomere length. As expected, Raw Fiber length was dependent on tibiotarsal angle (P < 0.0005 for all muscles, r2 range 0.22-0.61), while sarcomere length normalization eliminated the joint-angle dependent variation in Fiber length (P > 0.24, r2 range 0.001-0.028). Similarly, one-way ANOVA revealed no significant differences in normalized Fiber length among ankle angles for any of the three muscles (P > 0.1), regardless of animal size. To determine the resolution of the method, power calculations were performed. For all muscles studied, there was >90% chance of detecting a 15% Fiber length difference among muscles and >60% chance of detecting Fiber length differences as small as 10%. We thus conclude that the use of sarcomere length normalization in architectural studies permits resolution of Fiber length variations of 15% and may even be effective at resolving 10% Fiber length variations.

Hazizan Md Akil - One of the best experts on this subject based on the ideXlab platform.

  • Particulate composites based on ionic liquid-treated oil palm Fiber and thermoplastic starch adhesive
    Clean Technologies and Environmental Policy, 2016
    Co-Authors: Hamayoun Mahmood, Muhammad Moniruzzaman, Suzana Yusup, Hazizan Md Akil
    Abstract:

    Pretreatment of lignocellulosic materials is a highly essential and critical task for the manufacturing of engineered composite panels. Recently, ionic liquids (ILs) have emerged as a promising green solvent for lignocellulosic biomass disintegration. In this work, the impact of IL pretreatment on the flexural and thermal properties of the thermo-molded biocomposite panels made from oil palm biomass residue and thermoplastic starch biopolymer as binder was studied. Oil palm Fiber was pretreated with IL [emim][dep] (1-ethyl-3-methylimidazolium diethyl phosphate) and IL [bmim][Cl] (1-butyl-3-methylimidazolium chloride) prior to mixing with plasticized starch. The compounded mixture was then hot-pressed into composite panels. To understand the effect of IL pretreatment, lignocellulosic characterization, morphology, and thermogravimetric analysis of the untreated and treated Fibers were performed. It was found that thermal stability of the oil palm biomass and the biocomposites was improved after IL pretreatment due to partial removal of hemicellulose and lignin from Raw Fiber. Moreover, pretreated biocomposites exhibited superior strength and modulus as compared to that of untreated sample as evidenced from flexural testing. The study plainly demonstrates that IL-assisted pretreatment could be an extremely attractive and clean technology for the efficient use of agro-based industrial waste in biocomposite field.