The Experts below are selected from a list of 297 Experts worldwide ranked by ideXlab platform
Michael Famulok - One of the best experts on this subject based on the ideXlab platform.
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Supramolecular aptamer nano-constructs for Receptor-Mediated Targeting and light-triggered release of chemotherapeutics into cancer cells
Nature Communications, 2018Co-Authors: Deepak K. Prusty, Volker Adam, Stephan Irsen, Reza M Zadegan, Michael FamulokAbstract:Effective therapeutic platforms should combine serum stability, selective Targeting, and controlled drug release. Here, the authors self-assemble an aptamer-based nanoscaffold that contains separate cell-Targeting and photo-regulated drug-carrying domains, realizing multiple therapeutic functionalities in a single construct.AbstractPlatforms for targeted drug-delivery must simultaneously exhibit serum stability, efficient directed cell internalization, and triggered drug release. Here, using lipid-mediated self-assembly of aptamers, we combine multiple structural motifs into a single nanoconstruct that targets hepatocyte growth factor receptor (cMet). The nanocarrier consists of lipidated versions of a cMet-binding aptamer and a separate lipidated GC-rich DNA hairpin motif loaded with intercalated doxorubicin. Multiple 2′,6′-dimethylazobenzene moieties are incorporated into the doxorubicin-binding motif to trigger the release of the chemotherapeutics by photoisomerization. The lipidated DNA scaffolds self-assemble into spherical hybrid-nanoconstructs that specifically bind cMet. The combined features of the nanocarriers increase serum nuclease resistance, favor their import into cells presumably mediated by endocytosis, and allow selective photo-release of the chemotherapeutic into the targeted cells. cMet-expressing H1838 tumor cells specifically internalize drug-loaded nanoconstructs, and subsequent UV exposure enhances cell mortality. This modular approach thus paves the way for novel classes of powerful aptamer-based therapeutics.
Deepak K. Prusty - One of the best experts on this subject based on the ideXlab platform.
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Supramolecular aptamer nano-constructs for Receptor-Mediated Targeting and light-triggered release of chemotherapeutics into cancer cells
Nature Communications, 2018Co-Authors: Deepak K. Prusty, Volker Adam, Stephan Irsen, Reza M Zadegan, Michael FamulokAbstract:Effective therapeutic platforms should combine serum stability, selective Targeting, and controlled drug release. Here, the authors self-assemble an aptamer-based nanoscaffold that contains separate cell-Targeting and photo-regulated drug-carrying domains, realizing multiple therapeutic functionalities in a single construct.AbstractPlatforms for targeted drug-delivery must simultaneously exhibit serum stability, efficient directed cell internalization, and triggered drug release. Here, using lipid-mediated self-assembly of aptamers, we combine multiple structural motifs into a single nanoconstruct that targets hepatocyte growth factor receptor (cMet). The nanocarrier consists of lipidated versions of a cMet-binding aptamer and a separate lipidated GC-rich DNA hairpin motif loaded with intercalated doxorubicin. Multiple 2′,6′-dimethylazobenzene moieties are incorporated into the doxorubicin-binding motif to trigger the release of the chemotherapeutics by photoisomerization. The lipidated DNA scaffolds self-assemble into spherical hybrid-nanoconstructs that specifically bind cMet. The combined features of the nanocarriers increase serum nuclease resistance, favor their import into cells presumably mediated by endocytosis, and allow selective photo-release of the chemotherapeutic into the targeted cells. cMet-expressing H1838 tumor cells specifically internalize drug-loaded nanoconstructs, and subsequent UV exposure enhances cell mortality. This modular approach thus paves the way for novel classes of powerful aptamer-based therapeutics.
Abraham J. Domb - One of the best experts on this subject based on the ideXlab platform.
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Polysaccharide-Based Conjugates for Biomedical Applications
Bioconjugate Chemistry, 2015Co-Authors: Arijit Basu, Ester Abtew, Konda Reddy Kunduru, Abraham J. DombAbstract:Polysaccharides contain different functional groups (such as hydroxyl, amino, carboxylic acid, aldehydes) that make them ideal for conjugation. They are biodegradable, biocompatible, and hydrophilic. Polysaccharide conjugates have been used in drug, gene, and macromolecule delivery, tissue engineering, and other biomedical applications. Polysaccharide conjugates have also been used primarily for solubilization and controlled release of hydrophobic moieties. The advent of nanotechnology, gene therapy, and tissue engineering influenced the way these conjugates are now used. Modern day conjugates are modulated to be thermoresponsive, pH-responsive, photoresponsive, or target-specific (receptor mediated Targeting). This Review briefly introduces different polysaccharides followed by different synthetic strategies used for conjugation; finally, recent applications were compiled.
Laixi Wang - One of the best experts on this subject based on the ideXlab platform.
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chemoenzymatic synthesis and receptor binding of mannose 6 phosphate m6p containing glycoprotein ligands reveal unusual structural requirements for m6p receptor recognition
Journal of the American Chemical Society, 2016Co-Authors: Takahiro Yamaguchi, Mohammed N Amin, Christian Toonstra, Laixi WangAbstract:Mannose-6-phosphate (M6P)-terminated oligosaccharides are important signals for M6P-Receptor-Mediated Targeting of newly synthesized hydrolases from Golgi to lysosomes, but the precise structural requirement for the M6P ligand–receptor recognition has not been fully understood due to the difficulties in obtaining homogeneous M6P-containing glycoproteins. We describe here a chemoenzymatic synthesis of homogeneous phosphoglycoproteins carrying natural M6P-containing N-glycans. The method includes the chemical synthesis of glycan oxazolines with varied number and location of the M6P moieties and their transfer to the GlcNAc-protein by an endoglycosynthase to provide homogeneous M6P-containing glycoproteins. Simultaneous attachment of two M6P-oligosaccahrides to a cyclic polypeptide was also accomplished to yield bivalent M6P-glycopeptides. Surface plasmon resonance binding studies reveal that a single M6P moiety located at the low α-1,3-branch of the oligomannose context is sufficient for a high-affinity bin...
Volker Adam - One of the best experts on this subject based on the ideXlab platform.
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Supramolecular aptamer nano-constructs for Receptor-Mediated Targeting and light-triggered release of chemotherapeutics into cancer cells
Nature Communications, 2018Co-Authors: Deepak K. Prusty, Volker Adam, Stephan Irsen, Reza M Zadegan, Michael FamulokAbstract:Effective therapeutic platforms should combine serum stability, selective Targeting, and controlled drug release. Here, the authors self-assemble an aptamer-based nanoscaffold that contains separate cell-Targeting and photo-regulated drug-carrying domains, realizing multiple therapeutic functionalities in a single construct.AbstractPlatforms for targeted drug-delivery must simultaneously exhibit serum stability, efficient directed cell internalization, and triggered drug release. Here, using lipid-mediated self-assembly of aptamers, we combine multiple structural motifs into a single nanoconstruct that targets hepatocyte growth factor receptor (cMet). The nanocarrier consists of lipidated versions of a cMet-binding aptamer and a separate lipidated GC-rich DNA hairpin motif loaded with intercalated doxorubicin. Multiple 2′,6′-dimethylazobenzene moieties are incorporated into the doxorubicin-binding motif to trigger the release of the chemotherapeutics by photoisomerization. The lipidated DNA scaffolds self-assemble into spherical hybrid-nanoconstructs that specifically bind cMet. The combined features of the nanocarriers increase serum nuclease resistance, favor their import into cells presumably mediated by endocytosis, and allow selective photo-release of the chemotherapeutic into the targeted cells. cMet-expressing H1838 tumor cells specifically internalize drug-loaded nanoconstructs, and subsequent UV exposure enhances cell mortality. This modular approach thus paves the way for novel classes of powerful aptamer-based therapeutics.