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R E Stall - One of the best experts on this subject based on the ideXlab platform.
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Characterization of two Recessive Genes controlling resistance to all races of bacterial spot in peppers
Theoretical and Applied Genetics, 2010Co-Authors: C. Eduardo Vallejos, Jeffrey B Jones, Gerald V Minsavage, R E Stall, Valerie Jones, Diana C. Schultz, Rosana Rodrigues, Lisa E. Olsen, Michael MazourekAbstract:Bacterial spot, one of the most damaging diseases of pepper, is caused by Xanthomonas euvesicatoria . This pathogen has worldwide distribution and it is particularly devastating in tropical and sub-tropical regions where high temperatures and frequent precipitation provide ideal conditions for disease development. Three dominant resistance Genes have been deployed singly and in combination in commercial cultivars, but have been rendered ineffectual by the high mutation rate or deletion of the corresponding cognate effector Genes. These Genes are missing in race P6, and their absence makes this race virulent on all commercial pepper cultivars. The breeding line ECW12346 is the only source of resistance to race P6 in Capsicum annuum , and displays a non-hypersensitive type of resistance. Characterization of this resistance has identified two Recessive Genes: bs5 and bs6 . Individual analysis of these Genes revealed that bs5 confers a greater level of resistance than bs6 at 25°C, but in combination they confer full resistance to P6 indicating at least additive gene action. Tests carried out at 30°C showed that both resistances are compromised to a significant extent, but in combination they provide almost full resistance to race P6 indicating a positive epistatic interaction at high temperatures. A scan of the pepper genome with restriction fragment length polymorphism and AFLP markers led to the identification of a set of AFLP markers for bs5 . Allele-specific primers for a PCR-based bs5 -marker have been developed to facilitate the genetic manipulation of this gene.
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a non hypersensitive resistance in pepper to the bacterial spot pathogen is associated with two Recessive Genes
Phytopathology, 2002Co-Authors: Jeffrey B Jones, Gerald V Minsavage, Pamela D Roberts, R R Johnson, C S Kousik, S Subramanian, R E StallAbstract:Jones, J. B., Minsavage, G. V., Roberts, P. D., Johnson, R. R., Kousik, C. S., Subramanian, S., and Stall, R. E. 2002. A non-hypersensitive resistance in pepper to the bacterial spot pathogen is associated with two Recessive Genes. Phytopathology 92:273-277. The pepper genotype, ECW-12346, was developed with bacterial spot resistance derived from Pep13, PI 271322, and ECW123 (Early Calwonder containing Bs1, Bs2, and Bs3 Genes). For genetic analysis of this resistance, ECW12346, ECW123, F1, F2, and backcrosses were inoculated with a pepper race 6 (P6) strain. Two Recessive Genes were identified that determined resistance. The Genes are designated bs5 and bs6 for the resistance derived from PI 271322 and Pep13, respectively. In greenhouse and field studies, ECW12346 was highly resistant, whereas ECW123 had significant defoliation. In growth-room studies, electrolyte leakage and population dynamics were determined. Following infiltration of both genotypes with 108 CFU/ml of a P6 strain, there was no rapid increase in electrolyte leakage within 72 h, whereas a rapid increase in electrolyte leakage occurred within 24 h when a similar concentration of a P3 strain (containing the avrBs2 gene) was infiltrated into the intercellular spaces of the leaf. When 105 CFU/ml of a P6 strain was infiltrated into leaves, complete tissue collapse was evident in ECW123 10 days later as determined by visual assessment and electrolyte leakage data, but no confluent necrosis was detected in ECW12346. Internal populations were at least two logarithmic units higher in ECW123 than in ECW12346. Therefore, ECW12346 inhibits population build-up without inducing the typical hypersensitive reaction characterized by an increase in electrolyte leakage.
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a non hypersensitive resistance in pepper to the bacterial spot pathogen is associated with two Recessive Genes
Phytopathology, 2002Co-Authors: Jeffrey B Jones, Gerald V Minsavage, Pamela D Roberts, R R Johnson, C S Kousik, S Subramanian, R E StallAbstract:ABSTRACT The pepper genotype, ECW-12346, was developed with bacterial spot resistance derived from Pep13, PI 271322, and ECW123 (Early Calwonder containing Bs1, Bs2, and Bs3 Genes). For genetic analysis of this resistance, ECW12346, ECW123, F(1), F(2), and backcrosses were inoculated with a pepper race 6 (P6) strain. Two Recessive Genes were identified that determined resistance. The Genes are designated bs5 and bs6 for the resistance derived from PI 271322 and Pep13, respectively. In greenhouse and field studies, ECW12346 was highly resistant, whereas ECW123 had significant defoliation. In growth-room studies, electrolyte leakage and population dynamics were determined. Following infiltration of both genotypes with 10(8) CFU/ml of a P6 strain, there was no rapid increase in electrolyte leakage within 72 h, whereas a rapid increase in electrolyte leakage occurred within 24 h when a similar concentration of a P3 strain (containing the avrBs2 gene) was infiltrated into the intercellular spaces of the leaf. When 10(5) CFU/ml of a P6 strain was infiltrated into leaves, complete tissue collapse was evident in ECW123 10 days later as determined by visual assessment and electrolyte leakage data, but no confluent necrosis was detected in ECW12346. Internal populations were at least two logarithmic units higher in ECW123 than in ECW12346. Therefore, ECW12346 inhibits population build-up without inducing the typical hypersensitive reaction characterized by an increase in electrolyte leakage.
Jeffrey B Jones - One of the best experts on this subject based on the ideXlab platform.
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Characterization of two Recessive Genes controlling resistance to all races of bacterial spot in peppers
Theoretical and Applied Genetics, 2010Co-Authors: C. Eduardo Vallejos, Jeffrey B Jones, Gerald V Minsavage, R E Stall, Valerie Jones, Diana C. Schultz, Rosana Rodrigues, Lisa E. Olsen, Michael MazourekAbstract:Bacterial spot, one of the most damaging diseases of pepper, is caused by Xanthomonas euvesicatoria . This pathogen has worldwide distribution and it is particularly devastating in tropical and sub-tropical regions where high temperatures and frequent precipitation provide ideal conditions for disease development. Three dominant resistance Genes have been deployed singly and in combination in commercial cultivars, but have been rendered ineffectual by the high mutation rate or deletion of the corresponding cognate effector Genes. These Genes are missing in race P6, and their absence makes this race virulent on all commercial pepper cultivars. The breeding line ECW12346 is the only source of resistance to race P6 in Capsicum annuum , and displays a non-hypersensitive type of resistance. Characterization of this resistance has identified two Recessive Genes: bs5 and bs6 . Individual analysis of these Genes revealed that bs5 confers a greater level of resistance than bs6 at 25°C, but in combination they confer full resistance to P6 indicating at least additive gene action. Tests carried out at 30°C showed that both resistances are compromised to a significant extent, but in combination they provide almost full resistance to race P6 indicating a positive epistatic interaction at high temperatures. A scan of the pepper genome with restriction fragment length polymorphism and AFLP markers led to the identification of a set of AFLP markers for bs5 . Allele-specific primers for a PCR-based bs5 -marker have been developed to facilitate the genetic manipulation of this gene.
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a non hypersensitive resistance in pepper to the bacterial spot pathogen is associated with two Recessive Genes
Phytopathology, 2002Co-Authors: Jeffrey B Jones, Gerald V Minsavage, Pamela D Roberts, R R Johnson, C S Kousik, S Subramanian, R E StallAbstract:Jones, J. B., Minsavage, G. V., Roberts, P. D., Johnson, R. R., Kousik, C. S., Subramanian, S., and Stall, R. E. 2002. A non-hypersensitive resistance in pepper to the bacterial spot pathogen is associated with two Recessive Genes. Phytopathology 92:273-277. The pepper genotype, ECW-12346, was developed with bacterial spot resistance derived from Pep13, PI 271322, and ECW123 (Early Calwonder containing Bs1, Bs2, and Bs3 Genes). For genetic analysis of this resistance, ECW12346, ECW123, F1, F2, and backcrosses were inoculated with a pepper race 6 (P6) strain. Two Recessive Genes were identified that determined resistance. The Genes are designated bs5 and bs6 for the resistance derived from PI 271322 and Pep13, respectively. In greenhouse and field studies, ECW12346 was highly resistant, whereas ECW123 had significant defoliation. In growth-room studies, electrolyte leakage and population dynamics were determined. Following infiltration of both genotypes with 108 CFU/ml of a P6 strain, there was no rapid increase in electrolyte leakage within 72 h, whereas a rapid increase in electrolyte leakage occurred within 24 h when a similar concentration of a P3 strain (containing the avrBs2 gene) was infiltrated into the intercellular spaces of the leaf. When 105 CFU/ml of a P6 strain was infiltrated into leaves, complete tissue collapse was evident in ECW123 10 days later as determined by visual assessment and electrolyte leakage data, but no confluent necrosis was detected in ECW12346. Internal populations were at least two logarithmic units higher in ECW123 than in ECW12346. Therefore, ECW12346 inhibits population build-up without inducing the typical hypersensitive reaction characterized by an increase in electrolyte leakage.
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a non hypersensitive resistance in pepper to the bacterial spot pathogen is associated with two Recessive Genes
Phytopathology, 2002Co-Authors: Jeffrey B Jones, Gerald V Minsavage, Pamela D Roberts, R R Johnson, C S Kousik, S Subramanian, R E StallAbstract:ABSTRACT The pepper genotype, ECW-12346, was developed with bacterial spot resistance derived from Pep13, PI 271322, and ECW123 (Early Calwonder containing Bs1, Bs2, and Bs3 Genes). For genetic analysis of this resistance, ECW12346, ECW123, F(1), F(2), and backcrosses were inoculated with a pepper race 6 (P6) strain. Two Recessive Genes were identified that determined resistance. The Genes are designated bs5 and bs6 for the resistance derived from PI 271322 and Pep13, respectively. In greenhouse and field studies, ECW12346 was highly resistant, whereas ECW123 had significant defoliation. In growth-room studies, electrolyte leakage and population dynamics were determined. Following infiltration of both genotypes with 10(8) CFU/ml of a P6 strain, there was no rapid increase in electrolyte leakage within 72 h, whereas a rapid increase in electrolyte leakage occurred within 24 h when a similar concentration of a P3 strain (containing the avrBs2 gene) was infiltrated into the intercellular spaces of the leaf. When 10(5) CFU/ml of a P6 strain was infiltrated into leaves, complete tissue collapse was evident in ECW123 10 days later as determined by visual assessment and electrolyte leakage data, but no confluent necrosis was detected in ECW12346. Internal populations were at least two logarithmic units higher in ECW123 than in ECW12346. Therefore, ECW12346 inhibits population build-up without inducing the typical hypersensitive reaction characterized by an increase in electrolyte leakage.
D F Rong - One of the best experts on this subject based on the ideXlab platform.
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two Recessive Genes controlling thermophotoperiod sensitive male sterility in wheat
Theoretical and Applied Genetics, 2006Co-Authors: R X Guo, Dongfa Sun, Z B Tan, D F RongAbstract:Male sterility of wheat-breeding line 337S (Triticum aestivum L.) is sensitive to both short day-length/low temperature and long day-length/high temperature. 337S was crossed with the common wheat variety, Huamai No. 8 and the F1 was highly fertile. The F2 population segregated in a 15:1 ratio for fertility/sterility in 243 individuals under long day-length/high-temperature. The two thermophotoperiod-responsive male sterile Genes were mapped to chromosomes 5B and 2B using Simple Sequence Repeat (SSR) markers and bulked segregant analysis. Partial linkage maps around the sterility loci of chromosomes 2B and 5B were constructed using the 243 individuals in the F2 population. One gene (wptms1) for male sterility was flanked by the SSR markers Xgwm335 and Xgwm371 at a genetic distance in chromosome 5B of 4.1 and 24.4 cM, respectively. The second gene (wptms2) was mapped between markers Xgwm374 and Xgwm120 at a genetic distance of 6.6 and 20.9 cM, respectively. The closest linked markers Xgwm335 (wptms1) and Xgwm374 (wptms2) explained 53 and 38% of phenotypic variation for the fertility. The SSR markers provide a useful tool to transfer the male sterile Genes into elite wheat germplasm.
Gerald V Minsavage - One of the best experts on this subject based on the ideXlab platform.
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Characterization of two Recessive Genes controlling resistance to all races of bacterial spot in peppers
Theoretical and Applied Genetics, 2010Co-Authors: C. Eduardo Vallejos, Jeffrey B Jones, Gerald V Minsavage, R E Stall, Valerie Jones, Diana C. Schultz, Rosana Rodrigues, Lisa E. Olsen, Michael MazourekAbstract:Bacterial spot, one of the most damaging diseases of pepper, is caused by Xanthomonas euvesicatoria . This pathogen has worldwide distribution and it is particularly devastating in tropical and sub-tropical regions where high temperatures and frequent precipitation provide ideal conditions for disease development. Three dominant resistance Genes have been deployed singly and in combination in commercial cultivars, but have been rendered ineffectual by the high mutation rate or deletion of the corresponding cognate effector Genes. These Genes are missing in race P6, and their absence makes this race virulent on all commercial pepper cultivars. The breeding line ECW12346 is the only source of resistance to race P6 in Capsicum annuum , and displays a non-hypersensitive type of resistance. Characterization of this resistance has identified two Recessive Genes: bs5 and bs6 . Individual analysis of these Genes revealed that bs5 confers a greater level of resistance than bs6 at 25°C, but in combination they confer full resistance to P6 indicating at least additive gene action. Tests carried out at 30°C showed that both resistances are compromised to a significant extent, but in combination they provide almost full resistance to race P6 indicating a positive epistatic interaction at high temperatures. A scan of the pepper genome with restriction fragment length polymorphism and AFLP markers led to the identification of a set of AFLP markers for bs5 . Allele-specific primers for a PCR-based bs5 -marker have been developed to facilitate the genetic manipulation of this gene.
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a non hypersensitive resistance in pepper to the bacterial spot pathogen is associated with two Recessive Genes
Phytopathology, 2002Co-Authors: Jeffrey B Jones, Gerald V Minsavage, Pamela D Roberts, R R Johnson, C S Kousik, S Subramanian, R E StallAbstract:Jones, J. B., Minsavage, G. V., Roberts, P. D., Johnson, R. R., Kousik, C. S., Subramanian, S., and Stall, R. E. 2002. A non-hypersensitive resistance in pepper to the bacterial spot pathogen is associated with two Recessive Genes. Phytopathology 92:273-277. The pepper genotype, ECW-12346, was developed with bacterial spot resistance derived from Pep13, PI 271322, and ECW123 (Early Calwonder containing Bs1, Bs2, and Bs3 Genes). For genetic analysis of this resistance, ECW12346, ECW123, F1, F2, and backcrosses were inoculated with a pepper race 6 (P6) strain. Two Recessive Genes were identified that determined resistance. The Genes are designated bs5 and bs6 for the resistance derived from PI 271322 and Pep13, respectively. In greenhouse and field studies, ECW12346 was highly resistant, whereas ECW123 had significant defoliation. In growth-room studies, electrolyte leakage and population dynamics were determined. Following infiltration of both genotypes with 108 CFU/ml of a P6 strain, there was no rapid increase in electrolyte leakage within 72 h, whereas a rapid increase in electrolyte leakage occurred within 24 h when a similar concentration of a P3 strain (containing the avrBs2 gene) was infiltrated into the intercellular spaces of the leaf. When 105 CFU/ml of a P6 strain was infiltrated into leaves, complete tissue collapse was evident in ECW123 10 days later as determined by visual assessment and electrolyte leakage data, but no confluent necrosis was detected in ECW12346. Internal populations were at least two logarithmic units higher in ECW123 than in ECW12346. Therefore, ECW12346 inhibits population build-up without inducing the typical hypersensitive reaction characterized by an increase in electrolyte leakage.
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a non hypersensitive resistance in pepper to the bacterial spot pathogen is associated with two Recessive Genes
Phytopathology, 2002Co-Authors: Jeffrey B Jones, Gerald V Minsavage, Pamela D Roberts, R R Johnson, C S Kousik, S Subramanian, R E StallAbstract:ABSTRACT The pepper genotype, ECW-12346, was developed with bacterial spot resistance derived from Pep13, PI 271322, and ECW123 (Early Calwonder containing Bs1, Bs2, and Bs3 Genes). For genetic analysis of this resistance, ECW12346, ECW123, F(1), F(2), and backcrosses were inoculated with a pepper race 6 (P6) strain. Two Recessive Genes were identified that determined resistance. The Genes are designated bs5 and bs6 for the resistance derived from PI 271322 and Pep13, respectively. In greenhouse and field studies, ECW12346 was highly resistant, whereas ECW123 had significant defoliation. In growth-room studies, electrolyte leakage and population dynamics were determined. Following infiltration of both genotypes with 10(8) CFU/ml of a P6 strain, there was no rapid increase in electrolyte leakage within 72 h, whereas a rapid increase in electrolyte leakage occurred within 24 h when a similar concentration of a P3 strain (containing the avrBs2 gene) was infiltrated into the intercellular spaces of the leaf. When 10(5) CFU/ml of a P6 strain was infiltrated into leaves, complete tissue collapse was evident in ECW123 10 days later as determined by visual assessment and electrolyte leakage data, but no confluent necrosis was detected in ECW12346. Internal populations were at least two logarithmic units higher in ECW123 than in ECW12346. Therefore, ECW12346 inhibits population build-up without inducing the typical hypersensitive reaction characterized by an increase in electrolyte leakage.
Jorg Schipper - One of the best experts on this subject based on the ideXlab platform.
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dominant and Recessive Genes involved in tumor cell invasion
Current Opinion in Cell Biology, 1991Co-Authors: Walter Birchmeier, Jurgen Behrens, Michael K Weidner, Uwe Frixen, Jorg SchipperAbstract:Abstract The past year has seen the discovery and further analysis of several Genes and protein products that are critically involved in the generation of invasive and metastatic tumor cells. Like oncoGenes and tumor suppressor Genes, the Genes responsible for invasive and metastatic phenotypes can function in a dominant or Recessive fashion. In this review, particular emphasis will be given to the dominantly acting Genes encoding the cell adhesion molecule CD44 and the motility factor scatter factor, and the Recessively acting Genes encoding the cell adhesion molecule E-cadherin and nm23.
