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Celso Omoto - One of the best experts on this subject based on the ideXlab platform.
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inheritance and fitness costs of spodoptera frugiperda lepidoptera noctuidae resistance to spinosad in brazil
Pest Management Science, 2018Co-Authors: Daniela M Okuma, Daniel Bernardi, Renato J Horikoshi, Oderlei Bernardi, Aline Priscilla Gomes Da Silva, Celso OmotoAbstract:BACKGROUND Spodoptera frugiperda is a pest of economically important crops in South America. In Brazil, this species is considered the most destructive pest of maize. Use of spinosyn insecticides in insect resistance management (IRM) has been one strategy to control this pest. In this study, we selected a strain of S. frugiperda resistant to spinosad and evaluated the inheritance and fitness costs of the resistance. RESULTS Estimated LC50 (concentration required to kill 50% of larvae) values were 0.011 and 9.80 µg cm-2 for the spinosad-susceptible (Sus) and -resistant (Spin-res) strains, respectively. This represents an 890-fold resistance ratio. LC50 values for Reciprocal Crosses were 0.18 and 0.14 µg cm-2 , indicating that resistance to spinosad is an autosomal incompletely recessive trait. BackCrosses of the F1 progeny from Reciprocal Crosses with the parental Spin-res strain showed a polygenic effect. The estimated minimum number of independent segregations was ∼ 2.45, indicating that resistance to spinosad is associated with multiple genes. In greenhouse assays, third-instar larvae from the Spin-res strain showed >92% survival on spinosad-treated maize. By contrast Sus and Reciprocal Crosses exhibited 0% and <5% survival, respectively, indicating that resistance is recessive. Life history studies to investigate the fitness cost of resistance revealed a 41% reduction in the rate of survival to adulthood and a 49% lower reproductive rate in the Spin-res strain compared with the Sus strain. CONCLUSIONS The autosomal, incompletely recessive and polygenic resistance to spinosad in S. frugiperda and the fitness costs associated with this resistance can be exploited in IRM strategies to preserve the lifetime of spinosad for control of S. frugiperda in Brazil. © 2017 Society of Chemical Industry.
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genetic basis of spodoptera frugiperda lepidoptera noctuidae resistance to the chitin synthesis inhibitor lufenuron
Pest Management Science, 2016Co-Authors: Antonio Rogerio Bezerra Do Nascimento, Juliano Ricardo Farias, Daniel Bernardi, Renato J Horikoshi, Celso OmotoAbstract:BACKGROUND An understanding of the genetic basis of insect resistance to insecticides is important for the establishment of insect resistance management (IRM) strategies. In this study we evaluated the inheritance pattern of resistance to the chitin synthesis inhibitor lufenuron in Spodoptera frugiperda. RESULTS The LC50 values (95% CI) were 0.23 µg lufenuron mL−1 water (ppm) (0.18–0.28) for the susceptible strain (SUS) and 210.6 µg mL−1 (175.90–258.10) for the lufenuron-resistant strain (LUF-R), based on diet-overlay bioassay. The resistance ratio was ≈ 915-fold. The LC50 values for Reciprocal Crosses were 4.89 µg mL−1 (3.79–5.97) for female LUF-R and male SUS and 5.74 µg mL−1 (4.70–6.91) for female SUS and male LUF-R, indicating that the inheritance of S. frugiperda resistance to lufenuron is an autosomal, incompletely recessive trait. BackCrosses of the progeny of Reciprocal Crosses with the parental LUF-R showed a polygenic effect. The estimated minimum number of independent segregations was in the 11.02 range, indicating that resistance to lufenuron is associated with multiple genes in S. frugiperda. CONCLUSIONS Based on genetic Crosses, the inheritance pattern of lufenuron resistance in S. frugiperda was autosomal, incompletely recessive and polygenic. Implications of this finding to IRM are discussed in this paper. © 2015 Society of Chemical Industry
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heranca da resistencia de spodoptera frugiperda j e smith lepidoptera noctuidae a lambda cialotrina
Neotropical Entomology, 2001Co-Authors: Gabriela I Diezrodriguez, Celso OmotoAbstract:Inheritance of lambda-cyhalothrin resistance in Spodoptera frugiperda (J.E. Smith) was investigated in a strain of S. frugiperda showing an intensity of resistance of approximately 13-fold. Results from Reciprocal Crosses between susceptible (S) and resistant (R) strains of S. frugiperda to lambda-cyhalothrin revealed that resistance was autossomal and conferred by an incompletely recessive gene. Then, backCrosses of F1 progenies to the R strain were performed to determine the number of genes involved in the resistance. From interpretation of chi-square analysis of responses of progenies from backCrosses suggested that the resistance was conferred by a single major gene under a influence of some minor genes.
Thiago G Lima - One of the best experts on this subject based on the ideXlab platform.
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genomic scans reveal multiple mito nuclear incompatibilities in population Crosses of the copepod tigriopus californicus
Evolution, 2019Co-Authors: Thiago G Lima, Ronald S Burton, Christopher S WillettAbstract:The evolution of intrinsic postzygotic isolation can be explained by the accumulation of Dobzhansky-Muller incompatibilities (DMI). Asymmetries in the levels of hybrid inviability and hybrid sterility are commonly observed between Reciprocal Crosses, a pattern that can result from the involvement of uniparentally inherited factors. The mitochondrial genome is one such factor that appears to participate in DMI in some Crosses but the frequency of its involvement versus biparentally inherited factors is unclear. Here we assess the relative importance of incompatibilities between nuclear factors (nuclear-nuclear) versus those between mitochondrial and nuclear factors (mito-nuclear) in a species that lacks sex chromosomes. We used a Pool-seq approach to survey three Crosses among genetically divergent populations of the copepod, Tigriopus californicus, for regions of the genome that are affected by hybrid inviability. Results from Reciprocal Crosses suggest that mito-nuclear incompatibilities are more common than nuclear-nuclear incompatibilities overall. These results suggest that in the presence of very high levels of nucleotide divergence between mtDNA haplotypes, mito-nuclear incompatibilities can be important for the evolution of intrinsic postzygotic isolation. This is particularly interesting considering this species lacks sex chromosomes, which have been shown to harbor a particularly high number of nuclear-nuclear DMI in several other species.
Christopher S Willett - One of the best experts on this subject based on the ideXlab platform.
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genomic scans reveal multiple mito nuclear incompatibilities in population Crosses of the copepod tigriopus californicus
Evolution, 2019Co-Authors: Thiago G Lima, Ronald S Burton, Christopher S WillettAbstract:The evolution of intrinsic postzygotic isolation can be explained by the accumulation of Dobzhansky-Muller incompatibilities (DMI). Asymmetries in the levels of hybrid inviability and hybrid sterility are commonly observed between Reciprocal Crosses, a pattern that can result from the involvement of uniparentally inherited factors. The mitochondrial genome is one such factor that appears to participate in DMI in some Crosses but the frequency of its involvement versus biparentally inherited factors is unclear. Here we assess the relative importance of incompatibilities between nuclear factors (nuclear-nuclear) versus those between mitochondrial and nuclear factors (mito-nuclear) in a species that lacks sex chromosomes. We used a Pool-seq approach to survey three Crosses among genetically divergent populations of the copepod, Tigriopus californicus, for regions of the genome that are affected by hybrid inviability. Results from Reciprocal Crosses suggest that mito-nuclear incompatibilities are more common than nuclear-nuclear incompatibilities overall. These results suggest that in the presence of very high levels of nucleotide divergence between mtDNA haplotypes, mito-nuclear incompatibilities can be important for the evolution of intrinsic postzygotic isolation. This is particularly interesting considering this species lacks sex chromosomes, which have been shown to harbor a particularly high number of nuclear-nuclear DMI in several other species.
Daniel Bernardi - One of the best experts on this subject based on the ideXlab platform.
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inheritance and fitness costs of spodoptera frugiperda lepidoptera noctuidae resistance to spinosad in brazil
Pest Management Science, 2018Co-Authors: Daniela M Okuma, Daniel Bernardi, Renato J Horikoshi, Oderlei Bernardi, Aline Priscilla Gomes Da Silva, Celso OmotoAbstract:BACKGROUND Spodoptera frugiperda is a pest of economically important crops in South America. In Brazil, this species is considered the most destructive pest of maize. Use of spinosyn insecticides in insect resistance management (IRM) has been one strategy to control this pest. In this study, we selected a strain of S. frugiperda resistant to spinosad and evaluated the inheritance and fitness costs of the resistance. RESULTS Estimated LC50 (concentration required to kill 50% of larvae) values were 0.011 and 9.80 µg cm-2 for the spinosad-susceptible (Sus) and -resistant (Spin-res) strains, respectively. This represents an 890-fold resistance ratio. LC50 values for Reciprocal Crosses were 0.18 and 0.14 µg cm-2 , indicating that resistance to spinosad is an autosomal incompletely recessive trait. BackCrosses of the F1 progeny from Reciprocal Crosses with the parental Spin-res strain showed a polygenic effect. The estimated minimum number of independent segregations was ∼ 2.45, indicating that resistance to spinosad is associated with multiple genes. In greenhouse assays, third-instar larvae from the Spin-res strain showed >92% survival on spinosad-treated maize. By contrast Sus and Reciprocal Crosses exhibited 0% and <5% survival, respectively, indicating that resistance is recessive. Life history studies to investigate the fitness cost of resistance revealed a 41% reduction in the rate of survival to adulthood and a 49% lower reproductive rate in the Spin-res strain compared with the Sus strain. CONCLUSIONS The autosomal, incompletely recessive and polygenic resistance to spinosad in S. frugiperda and the fitness costs associated with this resistance can be exploited in IRM strategies to preserve the lifetime of spinosad for control of S. frugiperda in Brazil. © 2017 Society of Chemical Industry.
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genetic basis of spodoptera frugiperda lepidoptera noctuidae resistance to the chitin synthesis inhibitor lufenuron
Pest Management Science, 2016Co-Authors: Antonio Rogerio Bezerra Do Nascimento, Juliano Ricardo Farias, Daniel Bernardi, Renato J Horikoshi, Celso OmotoAbstract:BACKGROUND An understanding of the genetic basis of insect resistance to insecticides is important for the establishment of insect resistance management (IRM) strategies. In this study we evaluated the inheritance pattern of resistance to the chitin synthesis inhibitor lufenuron in Spodoptera frugiperda. RESULTS The LC50 values (95% CI) were 0.23 µg lufenuron mL−1 water (ppm) (0.18–0.28) for the susceptible strain (SUS) and 210.6 µg mL−1 (175.90–258.10) for the lufenuron-resistant strain (LUF-R), based on diet-overlay bioassay. The resistance ratio was ≈ 915-fold. The LC50 values for Reciprocal Crosses were 4.89 µg mL−1 (3.79–5.97) for female LUF-R and male SUS and 5.74 µg mL−1 (4.70–6.91) for female SUS and male LUF-R, indicating that the inheritance of S. frugiperda resistance to lufenuron is an autosomal, incompletely recessive trait. BackCrosses of the progeny of Reciprocal Crosses with the parental LUF-R showed a polygenic effect. The estimated minimum number of independent segregations was in the 11.02 range, indicating that resistance to lufenuron is associated with multiple genes in S. frugiperda. CONCLUSIONS Based on genetic Crosses, the inheritance pattern of lufenuron resistance in S. frugiperda was autosomal, incompletely recessive and polygenic. Implications of this finding to IRM are discussed in this paper. © 2015 Society of Chemical Industry
Renato J Horikoshi - One of the best experts on this subject based on the ideXlab platform.
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inheritance and fitness costs of spodoptera frugiperda lepidoptera noctuidae resistance to spinosad in brazil
Pest Management Science, 2018Co-Authors: Daniela M Okuma, Daniel Bernardi, Renato J Horikoshi, Oderlei Bernardi, Aline Priscilla Gomes Da Silva, Celso OmotoAbstract:BACKGROUND Spodoptera frugiperda is a pest of economically important crops in South America. In Brazil, this species is considered the most destructive pest of maize. Use of spinosyn insecticides in insect resistance management (IRM) has been one strategy to control this pest. In this study, we selected a strain of S. frugiperda resistant to spinosad and evaluated the inheritance and fitness costs of the resistance. RESULTS Estimated LC50 (concentration required to kill 50% of larvae) values were 0.011 and 9.80 µg cm-2 for the spinosad-susceptible (Sus) and -resistant (Spin-res) strains, respectively. This represents an 890-fold resistance ratio. LC50 values for Reciprocal Crosses were 0.18 and 0.14 µg cm-2 , indicating that resistance to spinosad is an autosomal incompletely recessive trait. BackCrosses of the F1 progeny from Reciprocal Crosses with the parental Spin-res strain showed a polygenic effect. The estimated minimum number of independent segregations was ∼ 2.45, indicating that resistance to spinosad is associated with multiple genes. In greenhouse assays, third-instar larvae from the Spin-res strain showed >92% survival on spinosad-treated maize. By contrast Sus and Reciprocal Crosses exhibited 0% and <5% survival, respectively, indicating that resistance is recessive. Life history studies to investigate the fitness cost of resistance revealed a 41% reduction in the rate of survival to adulthood and a 49% lower reproductive rate in the Spin-res strain compared with the Sus strain. CONCLUSIONS The autosomal, incompletely recessive and polygenic resistance to spinosad in S. frugiperda and the fitness costs associated with this resistance can be exploited in IRM strategies to preserve the lifetime of spinosad for control of S. frugiperda in Brazil. © 2017 Society of Chemical Industry.
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genetic basis of spodoptera frugiperda lepidoptera noctuidae resistance to the chitin synthesis inhibitor lufenuron
Pest Management Science, 2016Co-Authors: Antonio Rogerio Bezerra Do Nascimento, Juliano Ricardo Farias, Daniel Bernardi, Renato J Horikoshi, Celso OmotoAbstract:BACKGROUND An understanding of the genetic basis of insect resistance to insecticides is important for the establishment of insect resistance management (IRM) strategies. In this study we evaluated the inheritance pattern of resistance to the chitin synthesis inhibitor lufenuron in Spodoptera frugiperda. RESULTS The LC50 values (95% CI) were 0.23 µg lufenuron mL−1 water (ppm) (0.18–0.28) for the susceptible strain (SUS) and 210.6 µg mL−1 (175.90–258.10) for the lufenuron-resistant strain (LUF-R), based on diet-overlay bioassay. The resistance ratio was ≈ 915-fold. The LC50 values for Reciprocal Crosses were 4.89 µg mL−1 (3.79–5.97) for female LUF-R and male SUS and 5.74 µg mL−1 (4.70–6.91) for female SUS and male LUF-R, indicating that the inheritance of S. frugiperda resistance to lufenuron is an autosomal, incompletely recessive trait. BackCrosses of the progeny of Reciprocal Crosses with the parental LUF-R showed a polygenic effect. The estimated minimum number of independent segregations was in the 11.02 range, indicating that resistance to lufenuron is associated with multiple genes in S. frugiperda. CONCLUSIONS Based on genetic Crosses, the inheritance pattern of lufenuron resistance in S. frugiperda was autosomal, incompletely recessive and polygenic. Implications of this finding to IRM are discussed in this paper. © 2015 Society of Chemical Industry
