The Experts below are selected from a list of 2373 Experts worldwide ranked by ideXlab platform
Jae-seong Lee - One of the best experts on this subject based on the ideXlab platform.
-
The genome of the harpacticoid copepod Tigriopus japonicus: Potential for its use in marine molecular ecotoxicology.
Aquatic toxicology (Amsterdam Netherlands), 2020Co-Authors: Chang-bum Jeong, Heum Gi Park, Bo-young Lee, Beom-soon Choi, Min-sub Kim, Jun Chul Park, Duck-hyun Kim, Minghua Wang, Jae-seong LeeAbstract:The copepod Tigriopus japonicus has been widely used as an experimental species in the field of ecotoxicology. We have sequenced and assembled the whole genome of T. japonicus with comparative analysis of gene families that represent detoxification phases in two additional public genomes of Tigriopus spp., namely, T. californicus and T. kingsejongensis. The total length of the T. japonicus assembled genome was 196.6 Mb with an N50 value of 10.65 Mb and consisted of 339 scaffolds and 25,143 annotated genes. The detoxification gene families encoding cytochrome P450s (CYP450s), glutathione S-transferases (GSTs), and ATP-binding cassette (ABC) proteins in Tigriopus spp. have shown species-dependent diversity in several gene sets, suggesting that these genes have undergone a species-specific expansion to increase their fitness to different marine habitats and environmental pressures. Our study will provide a better understanding of the detoxification system in Tigriopus spp. and will contribute to various areas of research, including ecotoxicology.
-
complete mitochondrial genome of the antarctic copepod Tigriopus kingsejongenesis harpacticoida harpacticidae
Mitochondrial DNA Part B, 2019Co-Authors: Daesik Hwang, Beom-soon Choi, Jeonghoon Han, Sanghee Kim, Minchul Lee, Jae-seong LeeAbstract:The complete mitochondrial genome was sequenced from the Antarctic harpacticoid copepod Tigriopus kingsejongensis. The sequenced genome size was 14,940 bp, possessing different gene order and conte...
-
Effects of temperature shifts on life parameters and expression of fatty acid synthesis and heat shock protein genes in temperate and Antarctic copepods Tigriopus japonicus and Tigriopus kingsejongensis
Polar Biology, 2018Co-Authors: Jeonghoon Han, Jun Chul Park, Sanghee Kim, Minchul Lee, Jae-seong LeeAbstract:Global warming, increase of the atmospheric temperature leading to changes in climate, is a growing environmental concern for all organisms including marine organisms, and many efforts have been made to reveal the potential adverse effects on the systematics of aquatic organisms in response to the temperature changes. To examine the effects of temperature shifts on copepods in temperate and polar regions, we compared the life parameters and gene expression profiles of the de novo lipogenesis (DNL) pathway and heat shock protein (hsp) genes in the temperate copepod Tigriopus japonicus (T. japonicus) and the Antarctic copepod Tigriopus kingsejongensis (T. kingsejongensis). The median lethal temperature (LT50) and no observed effect level (NOEL) in the temperate copepod T. japonicus were determined to be 35.3 and 32 °C, respectively, in response to a temperature increase of 2 °C a day. In the Antarctic copepod T. kingsejongensis, the LT50 and NOEL were determined to be 24.8 and 12 °C, respectively. In addition, delayed developmental time and impaired fecundity were observed (P < 0.05) in response to temperature changes in T. japonicus. T. japonicus DNL pathway genes were down-regulated in response to high temperature, whereas T. kingsejongensis DNL pathway genes showed up-regulation in response to high temperature, indicating that these two Tigriopus species have different modes of action in response to temperature shifts. In both copepods, transcription of heat shock proteins (hsps) was mostly up-regulated in response to temperature shifts, but it showed moderate expression at 15 °C for T. japonicus and 4 °C for T. kingsejongensis. These findings indicate temperature shift-mediated species-specific modulations of the DNL pathway and hsps gene expression, leading to alteration of lipid synthesis and chaperoning with deleterious effects on the life parameters of these two copepods.
-
effects of temperature changes on the generation of reactive oxygen species and the expression and activity of glutathione s transferases in two congeneric copepods Tigriopus japonicus and Tigriopus kingsejongensis
Fisheries Science, 2018Co-Authors: Jeonghoon Han, Eunjin Byeon, Chang-bum Jeong, Jae-seong LeeAbstract:We measured the generation of intracellular reactive oxygen species (ROS) and the enzymatic activity and expression of glutathione-S transferase (GST) in response to temperature changes in the temperate copepod Tigriopus japonicus and the Antarctic copepod Tigriopus kingsejongensis. The levels of ROS and GST enzymatic activity were slightly elevated (P < 0.05) in T. japonicus in response to temperature changes but not in T. kingsejongensis. Furthermore, transcriptional regulation of all the GST genes in T. japonicus was significantly downregulated (P < 0.05) in response to temperature elevation to 35 °C, whereas messenger RNA expression of GST genes in T. kingsejongensis showed a significant increase (P < 0.05) at 24 °C, indicating that these two related but geographically distinct Tigriopus species have different molecular responses to temperature elevation. Overall, these findings indicate that temperature changes modulate oxidative damage through different modes of action, leading to different types of antioxidant defense in two congeneric copepods.
-
Effects of water accommodated fractions (WAFs) of crude oil in two congeneric copepods Tigriopus sp.
Ecotoxicology and environmental safety, 2017Co-Authors: Jeonghoon Han, Il-chan Kim, Hui-su Kim, Sanghee Kim, Un-ki Hwang, Jae-seong LeeAbstract:Abstract Oil pollution has deleterious effects on marine ecosystems. However, the toxicity of crude oil towards Antarctic marine organisms has not been well studied. We compared the deleterious effects of water accommodated fractions (WAFs) of crude oil on reproduction, intracellular reactive oxygen species (ROS) levels, and antioxidant enzymatic activity in Antarctic (Tigriopus kingsejongensis) and temperate (Tigriopus japonicus) copepods. Reproductive rates of T. kingsejongensis and T. japonicus were significantly reduced (P
Daesik Hwang - One of the best experts on this subject based on the ideXlab platform.
-
complete mitochondrial genome of the antarctic copepod Tigriopus kingsejongenesis harpacticoida harpacticidae
Mitochondrial DNA Part B, 2019Co-Authors: Daesik Hwang, Beom-soon Choi, Jeonghoon Han, Sanghee Kim, Minchul Lee, Jae-seong LeeAbstract:The complete mitochondrial genome was sequenced from the Antarctic harpacticoid copepod Tigriopus kingsejongensis. The sequenced genome size was 14,940 bp, possessing different gene order and conte...
-
identification of xenobiotic biodegradation and metabolism related genes in the copepod Tigriopus japonicus whole transcriptome analysis
Marine Genomics, 2015Co-Authors: Hui-su Kim, Heum Gi Park, Daesik Hwang, Bo-young Lee, Jeonghoon Han, Eunji Won, Jae-seong LeeAbstract:In this study, the whole transcriptome of Tigriopus japonicus was sequenced using next generation sequencing technology. De novo assembly was performed using Trinity, which assembled 140,130 contigs. Transdecoder found 54,761 candidate coding contigs, 39,507 of which showed homology to other species covering 15,310 genes by BLAST analysis. Functional gene annotation was performed by Gene Ontology, InterProScan, and KEGG pathway analyses. In addition to various metabolism-related pathways, xenobiotic biodegradation and metabolism were other interesting pathways in T. japonicus. Transcripts encoding various enzymes (e.g. superoxide dismutase, heat shock protein, and peroxidases) in response to a variety of stimuli were identified, which might be useful candidate biomarkers for ecotoxicology studies.
-
Genome-wide identification of nuclear receptor (NR) superfamily genes in the copepod Tigriopus japonicus
BMC Genomics, 2014Co-Authors: Daesik Hwang, Jaesung Rhee, Bo-young Lee, Hui-su Kim, Minchul Lee, Do-hyun Kyung, Ae-son Om, Jae-seong LeeAbstract:Background: Nuclear receptors (NRs) are a large superfamily of proteins defined by a DNA-binding domain (DBD) and a ligand-binding domain (LBD). They function as transcriptional regulators to control expression of genes involved in development, homeostasis, and metabolism. The number of NRs differs from species to species, because of gene duplications and/or lineage-specific gene losses during metazoan evolution. Many NRs in arthropods interact with the ecdysteroid hormone and are involved in ecdysone-mediated signaling in arthropods. The nuclear receptor superfamily complement has been reported in several arthropods, including crustaceans, but not in copepods. We identified the entire NR repertoire of the copepod Tigriopus japonicus, which is an important marine model species for ecotoxicology and environmental genomics. Results: Using whole genome and transcriptome sequences, we identified a total of 31 nuclear receptors in the genome of T. japonicus. Nomenclature of the nuclear receptors was determined based on the sequence similarities of the DNA-binding domain (DBD) and ligand-binding domain (LBD). The 7 subfamilies of NRs separate into five major clades (subfamilies NR1, NR2, NR3, NR4, and NR5/6). Although the repertoire of NR members in, T. japonicus was similar to that reported for other arthropods, there was an expansion of the NR1 subfamily in Tigriopus japonicus. The twelve unique nuclear receptors identified in T. japonicus are members of NR1L. This expansion may be a unique lineage-specific feature of crustaceans. Interestingly, E78 and HR83, which are present in other arthropods, were absent from the genomes of T. japonicus and two congeneric copepod species (T. japonicus and Tigriopus californicus), suggesting copepod lineage-specific gene loss. Conclusions: We identified all NR receptors present in the copepod, T. japonicus. Knowledge of the copepod nuclear receptor repertoire will contribute to a better understanding of copepod- and crustacean-specific NR evolution.
-
acute toxicities of trace metals and common xenobiotics to the marine copepod Tigriopus japonicus evaluation of its use as a benchmark species for routine ecotoxicity tests in western pacific coastal regions
Environmental Toxicology, 2007Co-Authors: K W Lee, Sheikh Raisuddin, Daesik Hwang, H G Park, Jae-seong LeeAbstract:Marine copepods have recently been recognized as important organisms in ecotoxicity testing for regulatory purposes. The harpacticoid copepod Tigriopus japonicus has a wide geographical distribution along the coast in the Western Pacific including Japan, South Korea, Taiwan, and Hong Kong. This study evaluated the acute toxicity sensitivity profile of Tigriopus japonicus against 12 common toxic substances including six endocrine disrupting chemicals (EDCs), three biocides and three trace metals. Through standard acute toxicity test procedures, toxicity endpoints LC(50), LC(10), and no observed effect concentration (NOEC) of each chemical were obtained. Although T. japonicus depicted different sensitivities towards different chemicals, a dose-response relationship was consistent in all cases. T. japonicus was particularly sensitive to most of the EDCs, but relatively less sensitive to molinate (a thiocarbate herbicide). Across all tested chemicals, tributyltin (TBT) was the most toxic to the copepod with the LC(50), LC(10), and NOEC of 0.05, 0.03, and 0.02 mg/L, respectively. A comparison made with available data on acute toxicities of these chemicals to other marine copepod species revealed that T. japonicus is generally more sensitive to EDCs and in particular to TBT. We, therefore, strongly advocate that T. japonicus shall be adopted as a benchmark marine species for routine ecotoxicity testing and ecotoxicological studies in Western Pacific coasts.
-
acute toxicities of trace metals and common xenobiotics to the marine copepod Tigriopus japonicus evaluation of its use as a benchmark species for routine ecotoxicity tests in western pacific coastal regions
Environmental Toxicology, 2007Co-Authors: Sheikh Raisuddin, Daesik Hwang, H G ParkAbstract:Marine copepods have recently been recognized as important organisms in ecotoxicity testing for regulatory purposes. The harpacticoid copepod Tigriopus japonicus has a wide geographical distribution along the coast in the Western Pacific including Japan, South Korea, Taiwan, and Hong Kong. This study evaluated the acute toxicity sensitivity profile of Tigriopus japonicus against 12 common toxic substances including six endocrine disrupting chemicals (EDCs), three biocides and three trace metals. Through standard acute toxicity test procedures, toxicity endpoints LC50, LC10, and no observed effect concentration (NOEC) of each chemical were obtained. Although T. japonicus depicted different sensitivities towards different chemicals, a dose-response relationship was consistent in all cases. T. japonicus was particularly sensitive to most of the EDCs, but relatively less sensitive to molinate (a thiocarbate herbicide). Across all tested chemicals, tributyltin (TBT) was the most toxic to the copepod with the LC50, LC10, and NOEC of 0.05, 0.03, and 0.02 mg/L, respectively. A comparison made with available data on acute toxicities of these chemicals to other marine copepod species revealed that T. japonicus is generally more sensitive to EDCs and in particular to TBT. We, therefore, strongly advocate that T. japonicus shall be adopted as a benchmark marine species for routine ecotoxicity testing and ecotoxicological studies in Western Pacific coasts. © 2007 Wiley Periodicals, Inc. Environ Toxicol 22: 532–538, 2007.
Sheikh Raisuddin - One of the best experts on this subject based on the ideXlab platform.
-
acute toxicities of trace metals and common xenobiotics to the marine copepod Tigriopus japonicus evaluation of its use as a benchmark species for routine ecotoxicity tests in western pacific coastal regions
Environmental Toxicology, 2007Co-Authors: Sheikh Raisuddin, Daesik Hwang, H G ParkAbstract:Marine copepods have recently been recognized as important organisms in ecotoxicity testing for regulatory purposes. The harpacticoid copepod Tigriopus japonicus has a wide geographical distribution along the coast in the Western Pacific including Japan, South Korea, Taiwan, and Hong Kong. This study evaluated the acute toxicity sensitivity profile of Tigriopus japonicus against 12 common toxic substances including six endocrine disrupting chemicals (EDCs), three biocides and three trace metals. Through standard acute toxicity test procedures, toxicity endpoints LC50, LC10, and no observed effect concentration (NOEC) of each chemical were obtained. Although T. japonicus depicted different sensitivities towards different chemicals, a dose-response relationship was consistent in all cases. T. japonicus was particularly sensitive to most of the EDCs, but relatively less sensitive to molinate (a thiocarbate herbicide). Across all tested chemicals, tributyltin (TBT) was the most toxic to the copepod with the LC50, LC10, and NOEC of 0.05, 0.03, and 0.02 mg/L, respectively. A comparison made with available data on acute toxicities of these chemicals to other marine copepod species revealed that T. japonicus is generally more sensitive to EDCs and in particular to TBT. We, therefore, strongly advocate that T. japonicus shall be adopted as a benchmark marine species for routine ecotoxicity testing and ecotoxicological studies in Western Pacific coasts. © 2007 Wiley Periodicals, Inc. Environ Toxicol 22: 532–538, 2007.
-
acute toxicities of trace metals and common xenobiotics to the marine copepod Tigriopus japonicus evaluation of its use as a benchmark species for routine ecotoxicity tests in western pacific coastal regions
Environmental Toxicology, 2007Co-Authors: K W Lee, Sheikh Raisuddin, Daesik Hwang, H G Park, Jae-seong LeeAbstract:Marine copepods have recently been recognized as important organisms in ecotoxicity testing for regulatory purposes. The harpacticoid copepod Tigriopus japonicus has a wide geographical distribution along the coast in the Western Pacific including Japan, South Korea, Taiwan, and Hong Kong. This study evaluated the acute toxicity sensitivity profile of Tigriopus japonicus against 12 common toxic substances including six endocrine disrupting chemicals (EDCs), three biocides and three trace metals. Through standard acute toxicity test procedures, toxicity endpoints LC(50), LC(10), and no observed effect concentration (NOEC) of each chemical were obtained. Although T. japonicus depicted different sensitivities towards different chemicals, a dose-response relationship was consistent in all cases. T. japonicus was particularly sensitive to most of the EDCs, but relatively less sensitive to molinate (a thiocarbate herbicide). Across all tested chemicals, tributyltin (TBT) was the most toxic to the copepod with the LC(50), LC(10), and NOEC of 0.05, 0.03, and 0.02 mg/L, respectively. A comparison made with available data on acute toxicities of these chemicals to other marine copepod species revealed that T. japonicus is generally more sensitive to EDCs and in particular to TBT. We, therefore, strongly advocate that T. japonicus shall be adopted as a benchmark marine species for routine ecotoxicity testing and ecotoxicological studies in Western Pacific coasts.
-
the copepod Tigriopus a promising marine model organism for ecotoxicology and environmental genomics
Aquatic Toxicology, 2007Co-Authors: Sheikh Raisuddin, Kenneth M.y. Leung, Kevin W H Kwok, Daniel Schlenk, Jae-seong LeeAbstract:There is an increasing body of evidence to support the significant role of invertebrates in assessing impacts of environmental contaminants on marine ecosystems. Therefore, in recent years massive efforts have been directed to identify viable and ecologically relevant invertebrate toxicity testing models. Tigriopus, a harpacticoid copepod has a number of promising characteristics which make it a candidate worth consideration in such efforts. Tigriopus and other copepods are widely distributed and ecologically important organisms. Their position in marine food chains is very prominent, especially with regard to the transfer of energy. Copepods also play an important role in the transportation of aquatic pollutants across the food chains. In recent years there has been a phenomenal increase in the knowledge base of Tigriopus spp., particularly in the areas of their ecology, geophylogeny, genomics and their behavioural, biochemical and molecular responses following exposure to environmental stressors and chemicals. Sequences of a number of important marker genes have been studied in various Tigriopus spp., notably T. californicus and T. japonicus. These genes belong to normal biophysiological functions (e.g. electron transport system enzymes) as well as stress and toxic chemical exposure responses (heat shock protein 20, glutathione reductase, glutathione S-transferase). Recently, 40,740 expressed sequenced tags (ESTs) from T. japonicus, have been sequenced and of them, 5,673 ESTs showed significant hits (E-value, >1.0E-05) to the red flour beetle Tribolium genome database. Metals and organic pollutants such as antifouling agents, pesticides, polycyclic aromatic hydrocarbons (PAH) and polychrlorinated biphenyls (PCB) have shown reproducible biological responses when tested in Tigriopus spp. Promising results have been obtained when Tigriopus was used for assessment of risk associated with exposure to endocrine-disrupting chemicals (EDCs). Application of environmental gene expression techniques has allowed evaluation of transcriptional changes in T. japonicus with the ultimate aim of understanding the mechanisms of action of environmental stressors. Through a better understanding of toxicological mechanisms, ecotoxicologists may use this ecologically relevant species in risk assessment studies in marine systems. The combination of uses as a whole-animal bioassay and gene expression studies indicate that Tigriopus may serve as an excellent tool to evaluate the impacts of marine pollution throughout the coastal region. The purpose of this review is to illustrate the potential of using Tigriopus to fulfill the niche as an important invertebrate marine model organism for ecotoxicology and environmental genomics. In addition, the knowledge gaps and areas for further studies have also been discussed.
H G Park - One of the best experts on this subject based on the ideXlab platform.
-
acute toxicities of trace metals and common xenobiotics to the marine copepod Tigriopus japonicus evaluation of its use as a benchmark species for routine ecotoxicity tests in western pacific coastal regions
Environmental Toxicology, 2007Co-Authors: K W Lee, Sheikh Raisuddin, Daesik Hwang, H G Park, Jae-seong LeeAbstract:Marine copepods have recently been recognized as important organisms in ecotoxicity testing for regulatory purposes. The harpacticoid copepod Tigriopus japonicus has a wide geographical distribution along the coast in the Western Pacific including Japan, South Korea, Taiwan, and Hong Kong. This study evaluated the acute toxicity sensitivity profile of Tigriopus japonicus against 12 common toxic substances including six endocrine disrupting chemicals (EDCs), three biocides and three trace metals. Through standard acute toxicity test procedures, toxicity endpoints LC(50), LC(10), and no observed effect concentration (NOEC) of each chemical were obtained. Although T. japonicus depicted different sensitivities towards different chemicals, a dose-response relationship was consistent in all cases. T. japonicus was particularly sensitive to most of the EDCs, but relatively less sensitive to molinate (a thiocarbate herbicide). Across all tested chemicals, tributyltin (TBT) was the most toxic to the copepod with the LC(50), LC(10), and NOEC of 0.05, 0.03, and 0.02 mg/L, respectively. A comparison made with available data on acute toxicities of these chemicals to other marine copepod species revealed that T. japonicus is generally more sensitive to EDCs and in particular to TBT. We, therefore, strongly advocate that T. japonicus shall be adopted as a benchmark marine species for routine ecotoxicity testing and ecotoxicological studies in Western Pacific coasts.
-
acute toxicities of trace metals and common xenobiotics to the marine copepod Tigriopus japonicus evaluation of its use as a benchmark species for routine ecotoxicity tests in western pacific coastal regions
Environmental Toxicology, 2007Co-Authors: Sheikh Raisuddin, Daesik Hwang, H G ParkAbstract:Marine copepods have recently been recognized as important organisms in ecotoxicity testing for regulatory purposes. The harpacticoid copepod Tigriopus japonicus has a wide geographical distribution along the coast in the Western Pacific including Japan, South Korea, Taiwan, and Hong Kong. This study evaluated the acute toxicity sensitivity profile of Tigriopus japonicus against 12 common toxic substances including six endocrine disrupting chemicals (EDCs), three biocides and three trace metals. Through standard acute toxicity test procedures, toxicity endpoints LC50, LC10, and no observed effect concentration (NOEC) of each chemical were obtained. Although T. japonicus depicted different sensitivities towards different chemicals, a dose-response relationship was consistent in all cases. T. japonicus was particularly sensitive to most of the EDCs, but relatively less sensitive to molinate (a thiocarbate herbicide). Across all tested chemicals, tributyltin (TBT) was the most toxic to the copepod with the LC50, LC10, and NOEC of 0.05, 0.03, and 0.02 mg/L, respectively. A comparison made with available data on acute toxicities of these chemicals to other marine copepod species revealed that T. japonicus is generally more sensitive to EDCs and in particular to TBT. We, therefore, strongly advocate that T. japonicus shall be adopted as a benchmark marine species for routine ecotoxicity testing and ecotoxicological studies in Western Pacific coasts. © 2007 Wiley Periodicals, Inc. Environ Toxicol 22: 532–538, 2007.
Suzanne Edmands - One of the best experts on this subject based on the ideXlab platform.
-
Variation in tolerance to common marine pollutants among different populations in two species of the marine copepod Tigriopus.
Environmental science and pollution research international, 2015Co-Authors: Patrick Y. Sun, Kenneth M.y. Leung, Helen B. Foley, Vivien W.w. Bao, Suzanne EdmandsAbstract:Geographical variation in chemical tolerance within a species can significantly influence results of whole animal bioassays, yet a literature survey showed that the majority of articles using bioassays did not provide detail on the original field collection site of their test specimens confounding the ability for accurate replication and comparison of results. Biological variation as a result of population-specific tolerance, if not addressed, can be misinterpreted as experimental error. Our studies of two marine copepod species, Tigriopus japonicus and Tigriopus californicus, found significant intra- and inter-specific variation in tolerance to copper and tributyltin. Because both species tolerate copper concentrations orders of magnitude higher than those found in coastal waters, difference in copper tolerance may be a by-product of adaptation to other stressors such as high temperature. Controlling for inter-population tolerance variation will greatly strengthen the application of bioassays in chemical toxicity tests.
-
phylogeography of the rock pool copepod Tigriopus brevicornis harpacticoida in the northern north atlantic and its relationship to other species of the genus
Marine Biology, 2010Co-Authors: Lisa Handschumacher, Suzanne Edmands, Maria Bjoerk Steinarsdottir, Agnar IngolfssonAbstract:We investigated relationships among North Atlantic Tigriopus brevicornis populations and their relationships to Mediterranean T. fulvus and North American T. californicus, using crossing experiments and mitochondrial DNA sequencing. All T.brevicornis populations tested were interfertile, while interspecific crosses produced either no offspring or offspring that did not survive past the larval stage, with the exception of a few T. brevicornis × T. californicus crosses that produced mature adults. DNA sequencing of a fragment of mitochondrial cytochrome oxidase I (COI) showed that samples of T. brevicornis from Iceland, the Faroes, Ireland, Scotland and Nova Scotia formed a single shallow clade. In contrast, T. brevicornis from more southern populations in France and Portugal formed a clade with substantially greater branch lengths. Tigriopus brevicornis was monophyletic, and T. brevicornis plus Mediterranean T. fulvus were together also monophyletic. The phylogeography of T. brevicornis closely mirrored that found in T. californicus, with substantially reduced interpopulation divergence at northern latitudes. The known distribution of T. brevicornis in Iceland and the Faroes is shown and dispersal mechanisms and habitat selection briefly discussed.
-
Recombination in Interpopulation Hybrids of the Copepod Tigriopus californicus: Release of Beneficial Variation Despite Hybrid Breakdown
The Journal of heredity, 2008Co-Authors: Suzanne EdmandsAbstract:Crosses between divergent populations of the copepod Tigriopus californicus typically result in fitness reductions for both F 2 and backcross hybrids. Because females in this species lack chiasmatic meiosis, both recombinant and nonrecombinant backcross hybrids can be created. Recombinant hybrids were found to have significantly faster development time for both males and females in 2 pairs of crosses, indicating the creation of favorable gene combinations by disrupting parental linkage groups.
-
Microsatellite DNA markers for the intertidal copepod Tigriopus californicus
Molecular Ecology Notes, 2004Co-Authors: J. S. Harrison, D. L. Peterson, J. R. Swain, Suzanne EdmandsAbstract:Eleven microsatellite loci were isolated and characterized for the intertidal copepod Tigriopus californicus . Primers reliably amplify alleles in inbred lines derived from two divergent populations previously shown to have genetic distances of 18% for the mitochondrial cytochrome oxidase I gene. The 11 loci provided markers for eight of the species’ 12 chromosomes.