The Experts below are selected from a list of 45 Experts worldwide ranked by ideXlab platform

Weilin Wang - One of the best experts on this subject based on the ideXlab platform.

  • msx2 and bcl2 expressions in the development of anorectal malformations in ethylenethiourea induced rat embryos
    Experimental and Molecular Pathology, 2018
    Co-Authors: Xingchi Liang, Dan Liu, Huimin Jia, Weilin Wang
    Abstract:

    Abstract Background This study aimed to determine Msh homeobox 2 (MSX2) and B cell lymphoma-2 (BCL2) expression patterns during anorectal development in anorectal malformations (ARM) and normal rat embryos, with the goals of determining the role of MSX2 and BCL2 in ARM pathogenesis. Methods ARM was induced in rat embryos with ethylenethiourea administered to dams on gestational day 10 (GD10). Embryos were harvested by cesarean deliveries from GD14 to GD16. MSX2 and BCL2 expression was evaluated via immunohistochemical staining, immunofluorescence, western blotting and quantitative real-time polymerase chain reaction (qRT-PCR). Results Immunohistochemical staining of ARM embryos revealed that MSX2 was mainly expressed in the epithelium of the hindgut and urorectal septum (URS) on GD14. On GD15 and GD16, MSX2-immunolabeled cells were noted in the epithelium of the Rectum, Fistula and URS. However, in normal embryos, faint immunopositivity for MSX2 was demonstrated in the epithelium of the Rectum and URS from GD14 to GD16. As for BCL2, in normal embryos, BCL2-immunopositive cells were extensively expressed in the epithelium of the hindgut and URS on GD14 and GD15. In ARM embryos, weak immunopositivity for BCL2 was detected in the epithelium of hindgut and URS on GD14 and GD15. Immunofluorescence revealed that MSX2 and BCL2 colocalized in the hindgut. In ARM embryos, we observed more MSX2-positive than BCL2-positive cells on GD14; the normal embryos had the opposite pattern. Analyses by western blot and qRT-PCR showed that MSX2 protein and mRNA expression was significantly increased in ARM embryos compared with the normal embryos on GD15 and GD16 (p  Conclusion These results indicate that upregulation of MSX2 and downregulation of BCL2 during cloacal development into the Rectum and urethra might be related to the ARM development, and MSX2 promoted apoptosis through reduction of BCL2 expression during the development of anorectal development in ARM.

Wang Gan - One of the best experts on this subject based on the ideXlab platform.

  • On repairing vagina Fistula by removing petal of Rectum
    Chinese Journal of Laboratory Diagnosis, 2008
    Co-Authors: Wang Gan
    Abstract:

    Objective Objective This paper explores the clinical treatment results by means of removing petal of Rectum to repair the low and mid-level vagina Fistula.Methods First,design the removable petal of Rectum,free its mucosa completely and repair the mouth of Rectum Fistula without intense force.Results Twelve cases were successfully treated and cured by operation.They were paid a follow-up visit to for 1 to 3 years.There was no recurrences of that disease and no symptoms of incontinence of anus among them.Conclusion The treatment for the low and mid-level Rectum vagina Fistula by removing petal of Rectum is proved to be successful.The way of treatment can be popularized.It,s crucial to the success of the operation to have sufficuient preparations for intestinal tract before opertion and standardize the manipulations during operation.

Xingchi Liang - One of the best experts on this subject based on the ideXlab platform.

  • msx2 and bcl2 expressions in the development of anorectal malformations in ethylenethiourea induced rat embryos
    Experimental and Molecular Pathology, 2018
    Co-Authors: Xingchi Liang, Dan Liu, Huimin Jia, Weilin Wang
    Abstract:

    Abstract Background This study aimed to determine Msh homeobox 2 (MSX2) and B cell lymphoma-2 (BCL2) expression patterns during anorectal development in anorectal malformations (ARM) and normal rat embryos, with the goals of determining the role of MSX2 and BCL2 in ARM pathogenesis. Methods ARM was induced in rat embryos with ethylenethiourea administered to dams on gestational day 10 (GD10). Embryos were harvested by cesarean deliveries from GD14 to GD16. MSX2 and BCL2 expression was evaluated via immunohistochemical staining, immunofluorescence, western blotting and quantitative real-time polymerase chain reaction (qRT-PCR). Results Immunohistochemical staining of ARM embryos revealed that MSX2 was mainly expressed in the epithelium of the hindgut and urorectal septum (URS) on GD14. On GD15 and GD16, MSX2-immunolabeled cells were noted in the epithelium of the Rectum, Fistula and URS. However, in normal embryos, faint immunopositivity for MSX2 was demonstrated in the epithelium of the Rectum and URS from GD14 to GD16. As for BCL2, in normal embryos, BCL2-immunopositive cells were extensively expressed in the epithelium of the hindgut and URS on GD14 and GD15. In ARM embryos, weak immunopositivity for BCL2 was detected in the epithelium of hindgut and URS on GD14 and GD15. Immunofluorescence revealed that MSX2 and BCL2 colocalized in the hindgut. In ARM embryos, we observed more MSX2-positive than BCL2-positive cells on GD14; the normal embryos had the opposite pattern. Analyses by western blot and qRT-PCR showed that MSX2 protein and mRNA expression was significantly increased in ARM embryos compared with the normal embryos on GD15 and GD16 (p  Conclusion These results indicate that upregulation of MSX2 and downregulation of BCL2 during cloacal development into the Rectum and urethra might be related to the ARM development, and MSX2 promoted apoptosis through reduction of BCL2 expression during the development of anorectal development in ARM.

Wang Jingu - One of the best experts on this subject based on the ideXlab platform.

  • On Repairing Congenital Vagina Fistula by Removing Petal of Rectum
    Journal of Tissue Engineering and Reconstructive Surgery, 2006
    Co-Authors: Wang Jingu
    Abstract:

    Objective This paper explores the clinical treatment results by means of removing petal of Rectum to repair congenital vagina Fistula. Methods First, design the removable petal of Rectum, free its mucosa completely and repair the mouth of Rectum Fistula without intense force. Close the deficiency of Rectum wall as well as vagina wall respectively and sew them up at different levels. Results Twelve cases were successfully treated and cured by operation. They were paid a follow-up visit to for 1 to 9 years. There was no recurrences of that disease and no symptoms of incontinence of anus among them. Conclusion The treatment for the low-level congenital Rectum vagina Fistula by removing petal of Rectum is proved to be successful. This way of treatment can be popularized. It' s crucial to the success of operation to have sufficient preparations for intestinal tract before operation and standardize the manipulations during operation.

Huimin Jia - One of the best experts on this subject based on the ideXlab platform.

  • msx2 and bcl2 expressions in the development of anorectal malformations in ethylenethiourea induced rat embryos
    Experimental and Molecular Pathology, 2018
    Co-Authors: Xingchi Liang, Dan Liu, Huimin Jia, Weilin Wang
    Abstract:

    Abstract Background This study aimed to determine Msh homeobox 2 (MSX2) and B cell lymphoma-2 (BCL2) expression patterns during anorectal development in anorectal malformations (ARM) and normal rat embryos, with the goals of determining the role of MSX2 and BCL2 in ARM pathogenesis. Methods ARM was induced in rat embryos with ethylenethiourea administered to dams on gestational day 10 (GD10). Embryos were harvested by cesarean deliveries from GD14 to GD16. MSX2 and BCL2 expression was evaluated via immunohistochemical staining, immunofluorescence, western blotting and quantitative real-time polymerase chain reaction (qRT-PCR). Results Immunohistochemical staining of ARM embryos revealed that MSX2 was mainly expressed in the epithelium of the hindgut and urorectal septum (URS) on GD14. On GD15 and GD16, MSX2-immunolabeled cells were noted in the epithelium of the Rectum, Fistula and URS. However, in normal embryos, faint immunopositivity for MSX2 was demonstrated in the epithelium of the Rectum and URS from GD14 to GD16. As for BCL2, in normal embryos, BCL2-immunopositive cells were extensively expressed in the epithelium of the hindgut and URS on GD14 and GD15. In ARM embryos, weak immunopositivity for BCL2 was detected in the epithelium of hindgut and URS on GD14 and GD15. Immunofluorescence revealed that MSX2 and BCL2 colocalized in the hindgut. In ARM embryos, we observed more MSX2-positive than BCL2-positive cells on GD14; the normal embryos had the opposite pattern. Analyses by western blot and qRT-PCR showed that MSX2 protein and mRNA expression was significantly increased in ARM embryos compared with the normal embryos on GD15 and GD16 (p  Conclusion These results indicate that upregulation of MSX2 and downregulation of BCL2 during cloacal development into the Rectum and urethra might be related to the ARM development, and MSX2 promoted apoptosis through reduction of BCL2 expression during the development of anorectal development in ARM.