The Experts below are selected from a list of 171 Experts worldwide ranked by ideXlab platform
Maria Luisa Ruiz Del Castillo - One of the best experts on this subject based on the ideXlab platform.
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Phenylalanine ammonia-lyase, flavanone 3β-hydroxylase and flavonol synthase enzyme activity by a new in vitro assay method in berry fruits.
2013Co-Authors: Gema Flores, Fernando Moreno, Gracia Patricia Blanch, Maria Luisa Ruiz Del CastilloAbstract:An HPLC method for the determination of phenylalanine ammonia-lyase, flavanone 3β-hydroxylase and flavonol synthase enzyme activity is proposed. This method is based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis. Optimisation of the method considered kinetic studies to establish the incubation time to perform the assay. The method here described proved to be an interesting approach to measure the activities of the three enzymes simultaneously increasing the rapidity, selectivity and sensitivity over other exiting methods. The enzyme activity method developed was applied to strawberry, raspberry, blackberry, Redcurrant and blackcurrant fruits.
Gema Flores - One of the best experts on this subject based on the ideXlab platform.
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Phenylalanine ammonia-lyase, flavanone 3β-hydroxylase and flavonol synthase enzyme activity by a new in vitro assay method in berry fruits.
2013Co-Authors: Gema Flores, Fernando Moreno, Gracia Patricia Blanch, Maria Luisa Ruiz Del CastilloAbstract:An HPLC method for the determination of phenylalanine ammonia-lyase, flavanone 3β-hydroxylase and flavonol synthase enzyme activity is proposed. This method is based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis. Optimisation of the method considered kinetic studies to establish the incubation time to perform the assay. The method here described proved to be an interesting approach to measure the activities of the three enzymes simultaneously increasing the rapidity, selectivity and sensitivity over other exiting methods. The enzyme activity method developed was applied to strawberry, raspberry, blackberry, Redcurrant and blackcurrant fruits.
Roland W S Weber - One of the best experts on this subject based on the ideXlab platform.
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resistance of botrytis cinerea to multiple fungicides in northern german small fruit production
2011Co-Authors: Roland W S WeberAbstract:Abstract During the vegetation period 2010, 353 isolates of Botrytis cinerea from 23 Northern German strawberry, raspberry, highbush blueberry, and Redcurrant fields were examined for sensitivity to the benzimidazole derivative thiophanate-methyl and the dicarboximide iprodione, as well as five fungicides currently used against gray mold in Germany. Of all isolates, 40.5% were highly resistant to thiophanate-methyl, 64.0% to iprodione, 45.0% to fenhexamid, 76.8% to trifloxystrobin, 21.5% to boscalid, and 14.7% to cyprodinil. No high resistance to fludioxonil was observed but medium resistance was recorded to fludioxonil as well as cyprodinil (41.1 and 27.2% of all isolates, respectively). In all, 63 isolates were sensitive to all five of the currently registered botryticides whereas 43, 81, 94, 49, and 23 isolates were medium or highly resistant to one, two, three, four, and five fungicides, respectively. Isolates resistant to five fungicides in vitro were capable of causing fruit rot on wounded apple pre...
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resistance of botrytis cinerea to multiple fungicides in northern german small fruit production
2011Co-Authors: Roland W S WeberAbstract:During the vegetation period 2010, 353 isolates of Botrytis cinerea from 23 Northern German strawberry, raspberry, highbush blueberry, and Redcurrant fields were examined for sensitivity to the benzimidazole derivative thiophanate-methyl and the dicarboximide iprodione, as well as five fungicides currently used against gray mold in Germany. Of all isolates, 40.5% were highly resistant to thiophanate-methyl, 64.0% to iprodione, 45.0% to fenhexamid, 76.8% to trifloxystrobin, 21.5% to boscalid, and 14.7% to cyprodinil. No high resistance to fludioxonil was observed but medium resistance was recorded to fludioxonil as well as cyprodinil (41.1 and 27.2% of all isolates, respectively). In all, 63 isolates were sensitive to all five of the currently registered botryticides whereas 43, 81, 94, 49, and 23 isolates were medium or highly resistant to one, two, three, four, and five fungicides, respectively. Isolates resistant to five fungicides in vitro were capable of causing fruit rot on wounded apple pretreated with any one of the three commercially available products containing fenhexamid, pyraclostrobin plus boscalid, or cyprodinil plus fludioxonil. These results question the sustainability of the current gray mold control strategy relying exclusively on fungicides with specific, single-site modes of action.
Fernando Moreno - One of the best experts on this subject based on the ideXlab platform.
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Phenylalanine ammonia-lyase, flavanone 3β-hydroxylase and flavonol synthase enzyme activity by a new in vitro assay method in berry fruits.
2013Co-Authors: Gema Flores, Fernando Moreno, Gracia Patricia Blanch, Maria Luisa Ruiz Del CastilloAbstract:An HPLC method for the determination of phenylalanine ammonia-lyase, flavanone 3β-hydroxylase and flavonol synthase enzyme activity is proposed. This method is based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis. Optimisation of the method considered kinetic studies to establish the incubation time to perform the assay. The method here described proved to be an interesting approach to measure the activities of the three enzymes simultaneously increasing the rapidity, selectivity and sensitivity over other exiting methods. The enzyme activity method developed was applied to strawberry, raspberry, blackberry, Redcurrant and blackcurrant fruits.
Gracia Patricia Blanch - One of the best experts on this subject based on the ideXlab platform.
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Phenylalanine ammonia-lyase, flavanone 3β-hydroxylase and flavonol synthase enzyme activity by a new in vitro assay method in berry fruits.
2013Co-Authors: Gema Flores, Fernando Moreno, Gracia Patricia Blanch, Maria Luisa Ruiz Del CastilloAbstract:An HPLC method for the determination of phenylalanine ammonia-lyase, flavanone 3β-hydroxylase and flavonol synthase enzyme activity is proposed. This method is based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis. Optimisation of the method considered kinetic studies to establish the incubation time to perform the assay. The method here described proved to be an interesting approach to measure the activities of the three enzymes simultaneously increasing the rapidity, selectivity and sensitivity over other exiting methods. The enzyme activity method developed was applied to strawberry, raspberry, blackberry, Redcurrant and blackcurrant fruits.