The Experts below are selected from a list of 41580 Experts worldwide ranked by ideXlab platform
Anthony R. Borneman - One of the best experts on this subject based on the ideXlab platform.
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heterologous production of Raspberry ketone in the wine yeast saccharomyces cerevisiae via pathway engineering and synthetic enzyme fusion
Microbial Cell Factories, 2016Co-Authors: Natoiya D.r. Lloyd, Anthony R. Borneman, Isak S. PretoriusAbstract:Raspberry ketone is the primary aroma compound found in raspberries and naturally derived Raspberry ketone is a valuable flavoring agent. The economic incentives for the production of Raspberry ketone, combined with the very poor yields from plant tissue, therefore make this compound an excellent target for heterologous production in synthetically engineered microbial strains. A de novo pathway for the production of Raspberry ketone was assembled using four heterologous genes, encoding phenylalanine/tyrosine ammonia lyase, cinnamate-4-hydroxlase, coumarate-CoA ligase and benzalacetone synthase, in an industrial strain of Saccharomyces cerevisiae. Synthetic protein fusions were also explored as a means of increasing yields of the final product. The highest Raspberry ketone concentration achieved in minimal media exceeded 7.5 mg/L when strains were fed with 3 mM p-coumaric acid; or 2.8 mg/L for complete de novo synthesis, both of which utilized a coumarate-CoA ligase, benzalacetone synthase synthetic fusion protein that increased yields over fivefold compared to the native enzymes. In addition, this strain was shown to be able to produce significant amounts of Raspberry ketone in wine, with a Raspberry ketone titer of 3.5 mg/L achieved after aerobic fermentation of Chardonnay juice or 0.68 mg/L under anaerobic winemaking conditions. We have shown that it is possible to produce sensorially-relevant quantities of Raspberry ketone in an industrial heterologous host. This paves the way for further pathway optimization to provide an economical alternative to Raspberry ketone derived from plant sources.
Kazufumi Yazaki - One of the best experts on this subject based on the ideXlab platform.
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characterization of Raspberry ketone zingerone synthase catalyzing the alpha beta hydrogenation of phenylbutenones in Raspberry fruits
Biochemical and Biophysical Research Communications, 2011Co-Authors: Takao Koeduka, Junichi Mano, Bunta Watanabe, Jun Hiratake, Shiro Suzuki, Kazufumi YazakiAbstract:Abstract Phenylbutanone Raspberry ketone, accumulating in the mature fruits of Raspberry (Rubus idaeus), imparts the characteristic aroma to the fruits. Here we describe the isolation and characterization of Raspberry ketone/zingerone synthase 1 (RZS1), which catalyzed the NADPH-dependent reduction of 4-hydroxybenzalacetone and 3-methoxy-4-hydroxybenzalacetone to Raspberry ketone and zingerone (the latter not found in Raspberry), respectively. Its apparent Km values for 4-hydroxybenzalacetone and NADPH were 88 μM and 202 μM, respectively. RZS1 preferred 4-hydroxybenzalacetone to 3-methoxy-4-hydroxybenzalacetone as a substrate by a factor of 1.7, and showed a 6-fold preference for 4-hydroxybenzalacetone over p-coumaraldehyde, and no activity for coniferaldehyde. Expression analysis of the RZS1 gene throughout the plant revealed that its transcript level was highest in mature fruits. We conclude that RZS1 is responsible for hydrogenation of the α,β-unsaturated double bond of phenylbutenones, the final step of the Raspberry ketone biosynthesis, in the Raspberry fruits.
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Characterization of Raspberry ketone/zingerone synthase, catalyzing the alpha, beta-hydrogenation of phenylbutenones in Raspberry fruits
Biochemical and Biophysical Research Communications, 2011Co-Authors: Takao Koeduka, Junichi Mano, Bunta Watanabe, Jun Hiratake, Shiro Suzuki, Kazufumi YazakiAbstract:Abstract Phenylbutanone Raspberry ketone, accumulating in the mature fruits of Raspberry (Rubus idaeus), imparts the characteristic aroma to the fruits. Here we describe the isolation and characterization of Raspberry ketone/zingerone synthase 1 (RZS1), which catalyzed the NADPH-dependent reduction of 4-hydroxybenzalacetone and 3-methoxy-4-hydroxybenzalacetone to Raspberry ketone and zingerone (the latter not found in Raspberry), respectively. Its apparent Km values for 4-hydroxybenzalacetone and NADPH were 88 μM and 202 μM, respectively. RZS1 preferred 4-hydroxybenzalacetone to 3-methoxy-4-hydroxybenzalacetone as a substrate by a factor of 1.7, and showed a 6-fold preference for 4-hydroxybenzalacetone over p-coumaraldehyde, and no activity for coniferaldehyde. Expression analysis of the RZS1 gene throughout the plant revealed that its transcript level was highest in mature fruits. We conclude that RZS1 is responsible for hydrogenation of the α,β-unsaturated double bond of phenylbutenones, the final step of the Raspberry ketone biosynthesis, in the Raspberry fruits.
Natoiya D.r. Lloyd - One of the best experts on this subject based on the ideXlab platform.
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heterologous production of Raspberry ketone in the wine yeast saccharomyces cerevisiae via pathway engineering and synthetic enzyme fusion
Microbial Cell Factories, 2016Co-Authors: Natoiya D.r. Lloyd, Anthony R. Borneman, Isak S. PretoriusAbstract:Raspberry ketone is the primary aroma compound found in raspberries and naturally derived Raspberry ketone is a valuable flavoring agent. The economic incentives for the production of Raspberry ketone, combined with the very poor yields from plant tissue, therefore make this compound an excellent target for heterologous production in synthetically engineered microbial strains. A de novo pathway for the production of Raspberry ketone was assembled using four heterologous genes, encoding phenylalanine/tyrosine ammonia lyase, cinnamate-4-hydroxlase, coumarate-CoA ligase and benzalacetone synthase, in an industrial strain of Saccharomyces cerevisiae. Synthetic protein fusions were also explored as a means of increasing yields of the final product. The highest Raspberry ketone concentration achieved in minimal media exceeded 7.5 mg/L when strains were fed with 3 mM p-coumaric acid; or 2.8 mg/L for complete de novo synthesis, both of which utilized a coumarate-CoA ligase, benzalacetone synthase synthetic fusion protein that increased yields over fivefold compared to the native enzymes. In addition, this strain was shown to be able to produce significant amounts of Raspberry ketone in wine, with a Raspberry ketone titer of 3.5 mg/L achieved after aerobic fermentation of Chardonnay juice or 0.68 mg/L under anaerobic winemaking conditions. We have shown that it is possible to produce sensorially-relevant quantities of Raspberry ketone in an industrial heterologous host. This paves the way for further pathway optimization to provide an economical alternative to Raspberry ketone derived from plant sources.
Robert R. Martin - One of the best experts on this subject based on the ideXlab platform.
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A new member of the family Reoviridae may contribute to severe crumbly fruit in red Raspberry, Rubus idaeus 'Meeker'.
Julius-Kühn-Archiv, 2010Co-Authors: D. Quito, Wilhelm Jelkmann, S. Alt, S. Leible, Robert R. MartinAbstract:Abstract A virus induced crumbly fruit disease of considerable importance in ‘Meeker’ and other cultivars of red Raspberry has been observed in northern Washington, USA, and British Columbia, Canada and to a lesser extent in the Willamette Valley of Oregon. Raspberry bushy dwarf virus (RBDV), a pollen-borne virus, has been considered the causal agent of the disease. However, dsRNA extractions from Raspberry plants exhibiting severe crumbly fruit in northern Washington revealed multiple bands in addition to those corresponding to RBDV (5.5kb and 2.2kb). Sequence analyses of these dsRNAs showed the presence of two additional viruses. One has significant amino acid identity to proteins encoded by Rice ragged stunt virus (RRSV), a ten-segmented dsRNA Oryzavirus that belongs to the family Reoviridae . Thus far, all dsRNA segments, except the one that corresponds to S6 of RRSV, have been fully sequenced and found to have characteristic features of other plant reoviruses genomes. In addition, Raspberry leaf mottle virus (RLMV), a recently characterized member of the Closteroviridae , has also been identified from raspberries with severe crumbly fruit. These findings along with the lack of severe crumbly fruit symptoms in ‘Meeker’ red Raspberry singly infected with RBDV in Oregon, suggest the existence of a novel virus complex associated with severe crumbly fruit in red raspberries. The complex may involve RBDV, RLMV and/or this new reovirus, provisionally named Raspberry latent virus (RpLV). Keywords: Raspberry crumbly fruit, Raspberry bushy dwarf virus, Raspberry leaf mottle virus, Raspberry leaf spot virus, plant reoviruses.
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Identification and characterization of Raspberry mottle virus, a novel member of the Closteroviridae
Virus Research, 2007Co-Authors: Ioannis E. Tzanetakis, Anne Halgren, Nola J. Mosier, Robert R. MartinAbstract:Raspberry mosaic is one of the most important viral diseases of Raspberry. Four virus and virus-like agents, two of which are poorly characterized, have been implicated in the disease complex based on symptom development in Rubus indicators. Three novel viruses were identified in a red Raspberry plant that caused typical Raspberry mosaic symptoms when grafted onto indicators. This communication focuses on one of these viruses, Raspberry mottle virus (RMoV), a new member of the family Closteroviridae. The complete nucleotide sequence of RMoV has been determined and exceeds 17 kilobases encoding 10 genes. The genome organization of RMoV is similar to that of Beet yellows virus, the type member of the Closterovirus genus, and phylogenetic analysis using the polymerase conserved motifs and the heat shock protein 70 homolog revealed a close relationship of RMoV with Strawberry chlorotic fleck associated virus and Citrus tristeza virus, which suggests the possibility of an aphid vector. The virus was detected in symptomatic Raspberry plants in production areas in mixed infections with several other viruses, indicating that RMoV may impact Raspberry production.
Takao Koeduka - One of the best experts on this subject based on the ideXlab platform.
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characterization of Raspberry ketone zingerone synthase catalyzing the alpha beta hydrogenation of phenylbutenones in Raspberry fruits
Biochemical and Biophysical Research Communications, 2011Co-Authors: Takao Koeduka, Junichi Mano, Bunta Watanabe, Jun Hiratake, Shiro Suzuki, Kazufumi YazakiAbstract:Abstract Phenylbutanone Raspberry ketone, accumulating in the mature fruits of Raspberry (Rubus idaeus), imparts the characteristic aroma to the fruits. Here we describe the isolation and characterization of Raspberry ketone/zingerone synthase 1 (RZS1), which catalyzed the NADPH-dependent reduction of 4-hydroxybenzalacetone and 3-methoxy-4-hydroxybenzalacetone to Raspberry ketone and zingerone (the latter not found in Raspberry), respectively. Its apparent Km values for 4-hydroxybenzalacetone and NADPH were 88 μM and 202 μM, respectively. RZS1 preferred 4-hydroxybenzalacetone to 3-methoxy-4-hydroxybenzalacetone as a substrate by a factor of 1.7, and showed a 6-fold preference for 4-hydroxybenzalacetone over p-coumaraldehyde, and no activity for coniferaldehyde. Expression analysis of the RZS1 gene throughout the plant revealed that its transcript level was highest in mature fruits. We conclude that RZS1 is responsible for hydrogenation of the α,β-unsaturated double bond of phenylbutenones, the final step of the Raspberry ketone biosynthesis, in the Raspberry fruits.
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Characterization of Raspberry ketone/zingerone synthase, catalyzing the alpha, beta-hydrogenation of phenylbutenones in Raspberry fruits
Biochemical and Biophysical Research Communications, 2011Co-Authors: Takao Koeduka, Junichi Mano, Bunta Watanabe, Jun Hiratake, Shiro Suzuki, Kazufumi YazakiAbstract:Abstract Phenylbutanone Raspberry ketone, accumulating in the mature fruits of Raspberry (Rubus idaeus), imparts the characteristic aroma to the fruits. Here we describe the isolation and characterization of Raspberry ketone/zingerone synthase 1 (RZS1), which catalyzed the NADPH-dependent reduction of 4-hydroxybenzalacetone and 3-methoxy-4-hydroxybenzalacetone to Raspberry ketone and zingerone (the latter not found in Raspberry), respectively. Its apparent Km values for 4-hydroxybenzalacetone and NADPH were 88 μM and 202 μM, respectively. RZS1 preferred 4-hydroxybenzalacetone to 3-methoxy-4-hydroxybenzalacetone as a substrate by a factor of 1.7, and showed a 6-fold preference for 4-hydroxybenzalacetone over p-coumaraldehyde, and no activity for coniferaldehyde. Expression analysis of the RZS1 gene throughout the plant revealed that its transcript level was highest in mature fruits. We conclude that RZS1 is responsible for hydrogenation of the α,β-unsaturated double bond of phenylbutenones, the final step of the Raspberry ketone biosynthesis, in the Raspberry fruits.
