The Experts below are selected from a list of 279 Experts worldwide ranked by ideXlab platform

Anders Kristensen - One of the best experts on this subject based on the ideXlab platform.

  • high frame rate multi resonance imaging Refractometry with distributed feedback dye laser sensor
    Light-Science & Applications, 2015
    Co-Authors: Christoph Vannahme, Martin Dufva, Anders Kristensen
    Abstract:

    By using a highly sensitive distributed feedback dye laser sensors, scientists in Denmark achieve high-frame-rate (12 Hz) imaging Refractometry. Since labeling can affect the dynamics of small molecules, a label-free method of monitoring the motion of small molecules on dissolution in liquids is highly desired for exploring chemical, microfluidic and biological processes. Researchers at the Technical University of Denmark demonstrate a potential way to achieve this using imaging Refractometry that employs multi-wavelength distributed feedback dye lasers. These lasers consist of several areas with different grating periods; this results in several spectrally separated laser emission peaks. In addition to having a high imaging frame rate, the technique has the advantages of a low detection limit and no moving parts. Its effectiveness is demonstrated by monitoring the complex motion of sucrose molecules driven by dissolution, diffusion and convection.

  • High frame rate multi-resonance imaging Refractometry with distributed feedback dye laser sensor
    Light: Science & Applications, 2015
    Co-Authors: Christoph Vannahme, Martin Dufva, Anders Kristensen
    Abstract:

    High frame rate and highly sensitive imaging of refractive index changes on a surface is very promising for studying the dynamics of dissolution, mixing and biological processes without the need for labeling. Here, a highly sensitive distributed feedback (DFB) dye laser sensor for high frame rate imaging Refractometry without moving parts is presented. DFB dye lasers are low-cost and highly sensitive refractive index sensors. The unique multi-wavelength DFB laser structure presented here comprises several areas with different grating periods. Imaging in two dimensions of space is enabled by analyzing laser light from all areas in parallel with an imaging spectrometer. With this multi-resonance imaging Refractometry method, the spatial position in one direction is identified from the horizontal, i.e., spectral position of the multiple laser lines which is obtained from the spectrometer charged coupled device (CCD) array. The orthogonal spatial position is obtained from the vertical spatial position on the spectrometer CCD array as in established spatially resolved spectroscopy. Here, the imaging technique is demonstrated by monitoring the motion of small sucrose molecules upon dissolution of solid sucrose in water. The omission of moving parts improves the robustness of the imaging system and allows a very high frame rate of up to 12 Hz. By using a highly sensitive distributed feedback dye laser sensors, scientists in Denmark achieve high-frame-rate (12 Hz) imaging Refractometry. Since labeling can affect the dynamics of small molecules, a label-free method of monitoring the motion of small molecules on dissolution in liquids is highly desired for exploring chemical, microfluidic and biological processes. Researchers at the Technical University of Denmark demonstrate a potential way to achieve this using imaging Refractometry that employs multi-wavelength distributed feedback dye lasers. These lasers consist of several areas with different grating periods; this results in several spectrally separated laser emission peaks. In addition to having a high imaging frame rate, the technique has the advantages of a low detection limit and no moving parts. Its effectiveness is demonstrated by monitoring the complex motion of sucrose molecules driven by dissolution, diffusion and convection.

Christoph Vannahme - One of the best experts on this subject based on the ideXlab platform.

  • high frame rate multi resonance imaging Refractometry with distributed feedback dye laser sensor
    Light-Science & Applications, 2015
    Co-Authors: Christoph Vannahme, Martin Dufva, Anders Kristensen
    Abstract:

    By using a highly sensitive distributed feedback dye laser sensors, scientists in Denmark achieve high-frame-rate (12 Hz) imaging Refractometry. Since labeling can affect the dynamics of small molecules, a label-free method of monitoring the motion of small molecules on dissolution in liquids is highly desired for exploring chemical, microfluidic and biological processes. Researchers at the Technical University of Denmark demonstrate a potential way to achieve this using imaging Refractometry that employs multi-wavelength distributed feedback dye lasers. These lasers consist of several areas with different grating periods; this results in several spectrally separated laser emission peaks. In addition to having a high imaging frame rate, the technique has the advantages of a low detection limit and no moving parts. Its effectiveness is demonstrated by monitoring the complex motion of sucrose molecules driven by dissolution, diffusion and convection.

  • High frame rate multi-resonance imaging Refractometry with distributed feedback dye laser sensor
    Light: Science & Applications, 2015
    Co-Authors: Christoph Vannahme, Martin Dufva, Anders Kristensen
    Abstract:

    High frame rate and highly sensitive imaging of refractive index changes on a surface is very promising for studying the dynamics of dissolution, mixing and biological processes without the need for labeling. Here, a highly sensitive distributed feedback (DFB) dye laser sensor for high frame rate imaging Refractometry without moving parts is presented. DFB dye lasers are low-cost and highly sensitive refractive index sensors. The unique multi-wavelength DFB laser structure presented here comprises several areas with different grating periods. Imaging in two dimensions of space is enabled by analyzing laser light from all areas in parallel with an imaging spectrometer. With this multi-resonance imaging Refractometry method, the spatial position in one direction is identified from the horizontal, i.e., spectral position of the multiple laser lines which is obtained from the spectrometer charged coupled device (CCD) array. The orthogonal spatial position is obtained from the vertical spatial position on the spectrometer CCD array as in established spatially resolved spectroscopy. Here, the imaging technique is demonstrated by monitoring the motion of small sucrose molecules upon dissolution of solid sucrose in water. The omission of moving parts improves the robustness of the imaging system and allows a very high frame rate of up to 12 Hz. By using a highly sensitive distributed feedback dye laser sensors, scientists in Denmark achieve high-frame-rate (12 Hz) imaging Refractometry. Since labeling can affect the dynamics of small molecules, a label-free method of monitoring the motion of small molecules on dissolution in liquids is highly desired for exploring chemical, microfluidic and biological processes. Researchers at the Technical University of Denmark demonstrate a potential way to achieve this using imaging Refractometry that employs multi-wavelength distributed feedback dye lasers. These lasers consist of several areas with different grating periods; this results in several spectrally separated laser emission peaks. In addition to having a high imaging frame rate, the technique has the advantages of a low detection limit and no moving parts. Its effectiveness is demonstrated by monitoring the complex motion of sucrose molecules driven by dissolution, diffusion and convection.

Martin Dufva - One of the best experts on this subject based on the ideXlab platform.

  • high frame rate multi resonance imaging Refractometry with distributed feedback dye laser sensor
    Light-Science & Applications, 2015
    Co-Authors: Christoph Vannahme, Martin Dufva, Anders Kristensen
    Abstract:

    By using a highly sensitive distributed feedback dye laser sensors, scientists in Denmark achieve high-frame-rate (12 Hz) imaging Refractometry. Since labeling can affect the dynamics of small molecules, a label-free method of monitoring the motion of small molecules on dissolution in liquids is highly desired for exploring chemical, microfluidic and biological processes. Researchers at the Technical University of Denmark demonstrate a potential way to achieve this using imaging Refractometry that employs multi-wavelength distributed feedback dye lasers. These lasers consist of several areas with different grating periods; this results in several spectrally separated laser emission peaks. In addition to having a high imaging frame rate, the technique has the advantages of a low detection limit and no moving parts. Its effectiveness is demonstrated by monitoring the complex motion of sucrose molecules driven by dissolution, diffusion and convection.

  • High frame rate multi-resonance imaging Refractometry with distributed feedback dye laser sensor
    Light: Science & Applications, 2015
    Co-Authors: Christoph Vannahme, Martin Dufva, Anders Kristensen
    Abstract:

    High frame rate and highly sensitive imaging of refractive index changes on a surface is very promising for studying the dynamics of dissolution, mixing and biological processes without the need for labeling. Here, a highly sensitive distributed feedback (DFB) dye laser sensor for high frame rate imaging Refractometry without moving parts is presented. DFB dye lasers are low-cost and highly sensitive refractive index sensors. The unique multi-wavelength DFB laser structure presented here comprises several areas with different grating periods. Imaging in two dimensions of space is enabled by analyzing laser light from all areas in parallel with an imaging spectrometer. With this multi-resonance imaging Refractometry method, the spatial position in one direction is identified from the horizontal, i.e., spectral position of the multiple laser lines which is obtained from the spectrometer charged coupled device (CCD) array. The orthogonal spatial position is obtained from the vertical spatial position on the spectrometer CCD array as in established spatially resolved spectroscopy. Here, the imaging technique is demonstrated by monitoring the motion of small sucrose molecules upon dissolution of solid sucrose in water. The omission of moving parts improves the robustness of the imaging system and allows a very high frame rate of up to 12 Hz. By using a highly sensitive distributed feedback dye laser sensors, scientists in Denmark achieve high-frame-rate (12 Hz) imaging Refractometry. Since labeling can affect the dynamics of small molecules, a label-free method of monitoring the motion of small molecules on dissolution in liquids is highly desired for exploring chemical, microfluidic and biological processes. Researchers at the Technical University of Denmark demonstrate a potential way to achieve this using imaging Refractometry that employs multi-wavelength distributed feedback dye lasers. These lasers consist of several areas with different grating periods; this results in several spectrally separated laser emission peaks. In addition to having a high imaging frame rate, the technique has the advantages of a low detection limit and no moving parts. Its effectiveness is demonstrated by monitoring the complex motion of sucrose molecules driven by dissolution, diffusion and convection.

H D Tyler - One of the best experts on this subject based on the ideXlab platform.

  • estimate of serum immunoglobulin g concentration using Refractometry with or without caprylic acid fractionation
    Journal of Dairy Science, 2013
    Co-Authors: K M Morrill, Javier Polo, A Lago, Joy M Campbell, J D Quigley, H D Tyler
    Abstract:

    Abstract Objectives of this study were to develop a rapid calf-side test to determine serum IgG concentrations using caprylic acid (CA) fractionation, followed by Refractometry of the IgG-rich supernatant and compare the accuracy of this method with results obtained using Refractometry using raw serum. Serum samples (n=200) were obtained from 1-d-old calves, frozen (−20°C), and shipped to the laboratory. Samples were allowed to thaw for 1h at room temperature. Fractionation with CA was conducted by adding 1mL of serum to a tube containing 45, 60, or 75µL of CA and 0.5, 1.0, or 1.5mL of 0.06 M acetic acid. The tube contents were mixed well, allowed to react for 1 min, and then centrifuged at 3,300 × g for 0, 10, or 20 min at 25°C. The %Brix and refractive index of the fractionated supernatant were determined using a digital refractometer. Nonfractionated serum was analyzed for %Brix (BRn), refractive index (nDn), and IgG concentration by radial immunodiffusion. The mean serum IgG concentration was 19.0 mg/mL [standard deviation (SD)=9.7], with a range of 3.5 to 47.0 mg/mL. The mean serum BRn was 8.6 (SD=0.91), with a range of 6.8 to 11.0. The mean serum nDn was 1.34566 (SD=0.00140), with a range of 1.34300 to 1.34930. Serum nDn was positively correlated with IgG concentration (correlation coefficient=0.86; n=185). Fractionated samples treated with 1mL 0.6 M acetic acid and 60µL of CA and not centrifuged before analysis resulted in a strong relationship between the refractive index of the fractionated supernatant and IgG (correlation coefficient=0.80; n=45). Regression was used to determine cut points indicative of 10, 12, and 14 mg of IgG/mL to determine the sensitivity and specificity of Refractometry to identify failure of passive transfer (serum IgG

  • estimate of colostral immunoglobulin g concentration using Refractometry without or with caprylic acid fractionation
    Journal of Dairy Science, 2012
    Co-Authors: K M Morrill, Erin Conrad, Javier Polo, A Lago, Joy M Campbell, J D Quigley, H D Tyler
    Abstract:

    Our objectives were to evaluate the use of Refractometry as a means of estimating immunoglobulin G (IgG) concentration of bovine maternal colostrum (MC) and determine if fractionation of MC using caprylic acid (CA) improved estimates of IgG. Samples (n = 85) of MC were collected from a single dairy in California and used to determine the method of CA fraction that produced the best prediction of IgG based on CA fractionation followed by Refractometry. Subsequently, samples of MC (n = 827) were collected from 67 farms in 12 states to compare Refractometry with or without CA fractionation as methods to estimate IgG concentration. Samples were collected from the feeding pool and consisted of fresh (n = 196), previously frozen (n = 479), or refrigerated (n = 152) MC. Samples were further classified by the number freeze-thaw cycles before analysis. Fractionation with CA was conducted by adding 1 mL of MC to a tube containing 75 μL of CA and 1 mL of 0.06 M acetic acid. The tube was shaken and allowed to react for 1 min. Refractive index of the IgG-rich supernatant (nDf) was determined using a digital refractometer. Whole, nonfractionated MC was analyzed for IgG by radial immunodiffusion (RID) and refractive index (nDw). The relationship between nDf and IgG (r = 0.53; n = 805) was weak, whereas that between nDw and IgG was stronger (r = 0.73; n = 823). Fresh samples analyzed by Refractometry that subsequently went through 1 freeze-thaw cycle before RID analysis resulted in the strongest relationship between IgG and nDf or nDw (r = 0.93 and 0.90, respectively). The MC samples collected fresh on the farm but frozen 2 or more times before analysis by Refractometry or RID had low correlations between IgG and nDf and nDw (r = 0.09 and 0.01). Samples refrigerated or frozen on the farm before analysis had weaker relationships between RID and nDf or nDw (r = 0.38 to 0.80), regardless of the number of freeze-thaw cycles. Breed and lactation number did not affect the accuracy of either test. These results indicated that Refractometry, without or with CA fractionation, was an accurate and rapid method to determine IgG concentration when samples of MC were not previously stored before Refractometry and frozen only once before RID analysis.

P Ferdinand - One of the best experts on this subject based on the ideXlab platform.