The Experts below are selected from a list of 30 Experts worldwide ranked by ideXlab platform
Wang Shuanke - One of the best experts on this subject based on the ideXlab platform.
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guidance of Regenerating Axon by extracellular atp at peripheral nerve conduit an experimental study
Chinses Journal of Hand Surgery, 2002Co-Authors: Wang ShuankeAbstract:Objective To confirm the effect of extracellular ATP on the attractive guidance of the Regenerating Axon after injuries of the sciatic nerve in rats. Methods The model of the sciatic nerve defect (5 mm in length) was set up in SD female rats. A "Y shaped" silicone tube was used to bridge the defect. The single arm of the silicone tube was sutured to the proximal side of the sciatic nerve. At the left side, the distal two arms of the silicone tube were ligated, with injection of ATP into the left chamber (served as experimental group) and of physiological saline into another tube(control group).The model of the right side was similar to the left side except that the distal tube with injection of ATP was sutured with the distal side the sciatic nerve. After 4 and 8 weeks general observation of the Regenerating nerve was made. Light and electron microscopy examination were checked, and image analysis was done accordingly. The number of the Regenerating nerve fiber and the myelin thickness was examined. Results The Regenerating Axons were found only at the silicone tube containing the ATP. The sciatic nerve sutured with the distal arm of the Y tube can enhance the guidance of ATP and maturation of the Regenerating Axon.Conclusions Extracellular ATP has a powerful attraction to the Regenerating Axon of peripheral nerve. It can also promote maturation of the Regenerating Axon.
Gregory R. D. Evans - One of the best experts on this subject based on the ideXlab platform.
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Single-lumen and multi-lumen poly(ethylene glycol) nerve conduits fabricated by stereolithography for peripheral nerve regeneration in vivo
Journal of Reconstructive Microsurgery, 2015Co-Authors: Maristella S. Evangelista, Mireya Perez, Karina Arcaute, Ara A. Salibian, Jeffrey M. Hassan, Sean Darcy, Keyianoosh Z. Paydar, Brenda K. Mann, Ryan B. Wicker, Gregory R. D. EvansAbstract:Background The use of nerve conduits to facilitate nerve regrowth after peripheral nerve injury is limited to defects less than 3 cm. The purpose of this study is to determine the capability of novel single and multi-lumen poly(ethylene glycol) (PEG) conduits manufactured by stereolithography to promote peripheral nerve regeneration. Materials and Methods Eight Sprague Dawley rats with sharp transection injuries of the sciatic nerve were randomly assigned to receive single-lumen or multi-lumen PEG conduits to bridge a 10-mm gap. Sciatic nerve and conduit samples were harvested after 5 weeks, and Axon number, myelin thickness, fiber diameter, and g-ratio were analyzed. The contralateral intact nerve was also harvested for comparison. Results Partial nerve regeneration was observed in three out of four single-lumen conduits and one out of four multi-lumen conduits. Axon number in the single-lumen regenerated nerve approached that of the contralateral intact nerve at 4,492 ± 2,810.0 and 6,080 ± 627.9 fibers/mm(2), respectively. The percentage of small fibers was greater in the single-lumen conduit compared with the intact nerve, whereas myelin thickness and g-ratio were consistently greater in the autologous nerve. Axon regrowth through the multi-lumen conduits was severely limited. Conclusion Single-lumen stereolithography-manufactured PEG nerve conduits promote nerve regeneration, with Regenerating Axon numbers approaching that of normal nerve. Multi-lumen conduits demonstrated significantly less nerve regeneration, possibly due to physical properties of the conduit inhibiting growth. Further studies are necessary to compare the efficacy of the two conduits for functional recovery and to elucidate the reasons underlying their differences in nerve regeneration potential.
Maristella S. Evangelista - One of the best experts on this subject based on the ideXlab platform.
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Single-lumen and multi-lumen poly(ethylene glycol) nerve conduits fabricated by stereolithography for peripheral nerve regeneration in vivo
Journal of Reconstructive Microsurgery, 2015Co-Authors: Maristella S. Evangelista, Mireya Perez, Karina Arcaute, Ara A. Salibian, Jeffrey M. Hassan, Sean Darcy, Keyianoosh Z. Paydar, Brenda K. Mann, Ryan B. Wicker, Gregory R. D. EvansAbstract:Background The use of nerve conduits to facilitate nerve regrowth after peripheral nerve injury is limited to defects less than 3 cm. The purpose of this study is to determine the capability of novel single and multi-lumen poly(ethylene glycol) (PEG) conduits manufactured by stereolithography to promote peripheral nerve regeneration. Materials and Methods Eight Sprague Dawley rats with sharp transection injuries of the sciatic nerve were randomly assigned to receive single-lumen or multi-lumen PEG conduits to bridge a 10-mm gap. Sciatic nerve and conduit samples were harvested after 5 weeks, and Axon number, myelin thickness, fiber diameter, and g-ratio were analyzed. The contralateral intact nerve was also harvested for comparison. Results Partial nerve regeneration was observed in three out of four single-lumen conduits and one out of four multi-lumen conduits. Axon number in the single-lumen regenerated nerve approached that of the contralateral intact nerve at 4,492 ± 2,810.0 and 6,080 ± 627.9 fibers/mm(2), respectively. The percentage of small fibers was greater in the single-lumen conduit compared with the intact nerve, whereas myelin thickness and g-ratio were consistently greater in the autologous nerve. Axon regrowth through the multi-lumen conduits was severely limited. Conclusion Single-lumen stereolithography-manufactured PEG nerve conduits promote nerve regeneration, with Regenerating Axon numbers approaching that of normal nerve. Multi-lumen conduits demonstrated significantly less nerve regeneration, possibly due to physical properties of the conduit inhibiting growth. Further studies are necessary to compare the efficacy of the two conduits for functional recovery and to elucidate the reasons underlying their differences in nerve regeneration potential.
Wim Rutten - One of the best experts on this subject based on the ideXlab platform.
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in vivo testing of a 3d bifurcating microchannel scaffold inducing separation of Regenerating Axon bundles in peripheral nerves
Journal of Neural Engineering, 2013Co-Authors: Irina I Stoyanova, Richard J A Van Wezel, Wim RuttenAbstract:Artificial nerve guidance channels enhance the regenerative effectiveness in an injured peripheral nerve but the existing design so far has been limited to basic straight tubes simply guiding the growth to bridge the gap. Hence, one of the goals in development of more effective neuroprostheses is to create bidirectional highly selective neuro–electronic interface between a prosthetic device and the severed nerve. A step towards improving selectivity for both recording and stimulation have been made with some recent in vitro studies which showed that three-dimensional (3D) bifurcating microchannels can separate neurites growing on a planar surface and bring them into contact with individual electrodes. Since the growing Axons in vivo have the innate tendency to group in bundles surrounded by connective tissue, one of the big challenges in neuro–prosthetic interface design is how to overcome it. Therefore, we performed experiments with 3D bifurcating guidance scaffolds implanted in the sciatic nerve of rats to test if this new channel architecture could trigger separation pattern of ingrowth also in vivo. Our results showed that this new method enabled the re-growth of neurites into channels with gradually diminished width (80, 40 and 20 µm) and facilitated the separation of the Axonal bundles with 91% success. It seems that the 3D bifurcating scaffold might contribute towards conveying detailed neural control and sensory feedback to users of prosthetic devices, and thus could improve the quality of their daily life.
Richard J A Van Wezel - One of the best experts on this subject based on the ideXlab platform.
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in vivo testing of a 3d bifurcating microchannel scaffold inducing separation of Regenerating Axon bundles in peripheral nerves
Journal of Neural Engineering, 2013Co-Authors: Irina I Stoyanova, Richard J A Van Wezel, Wim RuttenAbstract:Artificial nerve guidance channels enhance the regenerative effectiveness in an injured peripheral nerve but the existing design so far has been limited to basic straight tubes simply guiding the growth to bridge the gap. Hence, one of the goals in development of more effective neuroprostheses is to create bidirectional highly selective neuro–electronic interface between a prosthetic device and the severed nerve. A step towards improving selectivity for both recording and stimulation have been made with some recent in vitro studies which showed that three-dimensional (3D) bifurcating microchannels can separate neurites growing on a planar surface and bring them into contact with individual electrodes. Since the growing Axons in vivo have the innate tendency to group in bundles surrounded by connective tissue, one of the big challenges in neuro–prosthetic interface design is how to overcome it. Therefore, we performed experiments with 3D bifurcating guidance scaffolds implanted in the sciatic nerve of rats to test if this new channel architecture could trigger separation pattern of ingrowth also in vivo. Our results showed that this new method enabled the re-growth of neurites into channels with gradually diminished width (80, 40 and 20 µm) and facilitated the separation of the Axonal bundles with 91% success. It seems that the 3D bifurcating scaffold might contribute towards conveying detailed neural control and sensory feedback to users of prosthetic devices, and thus could improve the quality of their daily life.